Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 175,145 Δ1 bp 16.7% coding (39/1422 nt) clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913175,1440C.16.7% 618.9 / 129.4 210coding (38/1422 nt)clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (96/77);  new base . (17/18);  total (115/95)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 217 total aligned reads displayed.

TTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGC  >  NC_000913/175045‑175239
                                                                                                   |                                                                                                
ttCATGGAACGTGCGAGTAAAATGCCGGCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGc                                                                                                  >  2:2187540/1‑100 (MQ=255)
  cATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcg                                                                                                >  1:1058689/1‑100 (MQ=255)
    tGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcg                                                                                              <  1:1264753/100‑1 (MQ=255)
     ggAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcgc                                                                                             >  2:706801/1‑100 (MQ=255)
      gAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGcgcgcgg                                                                                          <  1:1530446/100‑2 (MQ=255)
        cgatctgAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGcgcgcggcc                                                                                        <  2:1559050/94‑4 (MQ=255)
        aCGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGcc                                                                                         <  2:1211201/100‑1 (MQ=255)
        aCGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGcgcgcggcc                                                                                        <  2:816384/100‑4 (MQ=255)
          gTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTg                                                                                       <  2:329234/100‑1 (MQ=255)
             cGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATCCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGa                                                                                    <  2:3230288/100‑1 (MQ=255)
             cGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGa                                                                                    <  2:3053273/100‑1 (MQ=255)
             cGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCgg                                                                                    <  1:2707166/100‑2 (MQ=255)
             cGAGTAAAATGCCGTCATCTTATATGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAACCAACGCACGCCGCTCGcc                                                                                         >  2:3194584/1‑95 (MQ=255)
              gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGAc                                                                                   >  2:1084723/1‑100 (MQ=255)
              gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                   <  2:3950197/100‑3 (MQ=255)
              gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                   <  1:2028522/100‑3 (MQ=255)
              gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                   <  1:1598177/100‑3 (MQ=255)
              gAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                   <  1:2867593/100‑3 (MQ=255)
               atctaaaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACg                                                                                  <  1:1160937/96‑1 (MQ=255)
               aGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACg                                                                                  <  2:573671/100‑1 (MQ=255)
               aGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggtg                                                                                  <  2:3600112/100‑4 (MQ=255)
               aGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggtg                                                                                  <  1:3116217/100‑4 (MQ=255)
                gTAAAAAGCCGTCATCTTATTTGTATGACCCATAAGTGATCACTGGACGACAACTGATCCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGcgccgc                                                                                 <  1:2150702/100‑1 (MQ=255)
                   aaaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACgagat                                                                              >  2:1160928/1‑96 (MQ=255)
                    aaTGCCGTCAGCTTAGTTGTATGACCAATAAGTGAACAGTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGACGCGCGCCTGCGACGCAGAc                                                                             >  2:428539/1‑100 (MQ=255)
                     attccttcatcaTCTTTGTATGACCAACAAGTGATCATTGGATGCAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACa                                                                            <  2:518791/94‑1 (MQ=255)
                        ccGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACt                                                                         <  2:1299508/100‑1 (MQ=255)
                           tCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGAt                                                                      >  1:343374/1‑100 (MQ=255)
                              tCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCg                                                                   >  2:107831/1‑100 (MQ=255)
                                ttATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                 >  1:460472/1‑100 (MQ=255)
                                ttATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGcc                                                                 <  1:2522517/100‑1 (MQ=255)
                                 tagttGTATGACCAATAGGTGTTAATTGGATGAAAACTGATACTCCCTCTTTCGAGACACCGCAGGCCGCGGGACTGCGACGCAGACAACTGATTCGCCa                                                                >  2:2719807/4‑100 (MQ=255)
                                 tATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCa                                                                <  2:3121701/100‑1 (MQ=255)
                                 tATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCa                                                                <  1:480935/100‑1 (MQ=255)
                                    ttGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAAct                                                             <  2:1726673/100‑1 (MQ=255)
                                     tGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActt                                                            >  1:813419/1‑100 (MQ=255)
                                     tGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActt                                                            >  1:1406441/1‑100 (MQ=255)
                                     tGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActt                                                            >  1:2691440/1‑100 (MQ=255)
                                      gTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtc                                                           >  2:3822810/1‑100 (MQ=255)
                                       tATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtct                                                          >  1:3188609/1‑100 (MQ=255)
                                         tGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTg                                                        <  2:1219846/100‑1 (MQ=255)
                                         tGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTg                                                        >  1:3234979/1‑100 (MQ=255)
                                           aCCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTTGCCAActtctcg                                                        >  2:1757364/1‑96 (MQ=255)
                                           aCCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAg                                                      <  1:3350778/100‑1 (MQ=255)
                                             cAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                    >  1:2752585/1‑100 (MQ=255)
                                              aaTAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcgc                                                   <  1:59983/100‑1 (MQ=255)
                                               aTAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGcgcg                                                  >  1:1662146/1‑100 (MQ=255)
                                                 aaGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGAt                                                <  2:2549383/100‑1 (MQ=255)
                                                  aGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATa                                               >  2:1549807/1‑100 (MQ=255)
                                                   gTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATaa                                              >  1:3855685/1‑100 (MQ=255)
                                                   gTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATaa                                              >  1:2301207/1‑100 (MQ=255)
                                                    tGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATaaa                                             >  1:2825544/1‑100 (MQ=255)
                                                      aTCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAc                                           <  1:975551/100‑1 (MQ=255)
                                                        cATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAccc                                         >  1:2740959/1‑100 (MQ=255)
                                                          ttGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCg                                       <  1:2358962/100‑1 (MQ=255)
                                                           tGGATGAAACCTGATAGTCCCTCTTTAGGAACACCGCAGGCCGCGCGCCTGCGACGCCGAGCACTAATTCGCCGACTTCTTGAGCGCGCTGACAcccact                                      >  2:1941862/1‑97 (MQ=255)
                                                            ggATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGtt                                     >  1:2971118/1‑100 (MQ=255)
                                                            ggATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGtt                                     >  1:412889/1‑100 (MQ=255)
                                                            ggATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGtt                                     >  2:1848936/1‑100 (MQ=255)
                                                             gATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTa                                    >  1:1312776/1‑100 (MQ=255)
                                                             gATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTa                                    <  2:1339242/100‑1 (MQ=255)
                                                               tGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGc                                  <  2:2931015/100‑1 (MQ=255)
                                                                gAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCCCGATAAAACCCCGTTAGcc                                 >  2:3592186/1‑100 (MQ=255)
                                                                      tGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTg                           <  1:3549476/100‑1 (MQ=255)
                                                                         tACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCATTAGCCATTTTGttt                        >  1:3887204/1‑100 (MQ=255)
                                                                          aCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTTGCCATTTTGTTTa                       <  2:2441831/100‑1 (MQ=255)
                                                                          aCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTa                       <  2:118092/100‑1 (MQ=255)
                                                                              ccTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggc                   >  1:3295519/1‑100 (MQ=255)
                                                                               ttccgatctaaCACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcg                  <  1:1458466/91‑1 (MQ=255)
                                                                               ctctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcg                  >  2:3708089/1‑100 (MQ=255)
                                                                               ctctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcg                  >  1:3713785/1‑100 (MQ=255)
                                                                               ctctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcg                  >  2:3068419/1‑100 (MQ=255)
                                                                                tctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcgg                 <  2:630412/100‑1 (MQ=255)
                                                                                 ctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                >  2:787300/1‑100 (MQ=255)
                                                                                 ctTGAGAAACACCCCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                <  2:3429404/100‑1 (MQ=255)
                                                                                    tgtctgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgt             >  2:1259512/10‑100 (MQ=38)
                                                                                    tAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgt             >  1:559307/1‑100 (MQ=255)
                                                                                    cttctgcgtcGCAGGCGCGCGGCCTGCGGCGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgt             <  1:3675497/91‑1 (MQ=37)
                                                                                     aGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtc            <  2:2825526/100‑1 (MQ=255)
                                                                                     aGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtc            >  2:2744564/1‑100 (MQ=255)
                                                                                      gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcg           >  2:2956276/1‑100 (MQ=255)
                                                                                      gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcg           >  1:109311/1‑100 (MQ=255)
                                                                                      gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcg           <  2:3759940/100‑1 (MQ=255)
                                                                                      gAAACACCGCAGGACGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcg           >  1:2655410/1‑100 (MQ=255)
                                                                                        aaCACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcgagatcggaa         >  2:1458457/1‑91 (MQ=255)
                                                                                        aaCACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcgtc         >  1:1738746/1‑100 (MQ=255)
                                                                                         gcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGtcgtcg        >  1:3401840/5‑100 (MQ=255)
                                                                                         gcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGtcgtcg        >  2:2058735/5‑100 (MQ=255)
                                                                                          cacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCgg       <  1:2290904/100‑1 (MQ=255)
                                                                                          cacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCgg       <  1:3571821/100‑1 (MQ=255)
                                                                                           gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      >  2:3114284/3‑100 (MQ=255)
                                                                                           gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      >  1:1908787/3‑100 (MQ=255)
                                                                                           gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      >  2:1982260/3‑100 (MQ=255)
                                                                                           gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      >  1:633268/3‑100 (MQ=255)
                                                                                           acCGCGGGCCGCGCGCCTGCGACGCAGACAACTGAGTCGTGAACTTCTTGAGCGCGATAAGACCACGTTAGCCATTTTGTTTATGGCGGCAGTAGTCGGc      <  2:2650394/100‑1 (MQ=255)
                                                                                           acCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      <  1:3616048/100‑1 (MQ=255)
                                                                                           acCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGc      >  2:235311/1‑100 (MQ=255)
                                                                                            tcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCa     >  1:940615/2‑100 (MQ=255)
                                                                                            tcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCa     >  1:2039972/2‑100 (MQ=255)
                                                                                               cAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGc  >  1:707226/1‑100 (MQ=255)
                                                                                                   |                                                                                                
TTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGC  >  NC_000913/175045‑175239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: