Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,874 Δ1 bp 16.1% intergenic (‑137/+31) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8730C.16.1% 469.9 / 90.9 161intergenic (‑136/+32)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (68/65);  new base . (12/14);  total (80/81)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.81e-01

Only 100 of 164 total aligned reads displayed.

TAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTC  >  NC_000913/2596775‑2596971
                                                                                                  |                                                                                                   
  ggtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAg                                                                                                  >  2:453765/1‑100 (MQ=255)
  ggtggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAg                                                                                                  <  1:3350458/100‑1 (MQ=255)
    tggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                 <  2:1299663/99‑1 (MQ=255)
      gtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGTCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                             >  1:96709/1‑100 (MQ=255)
       ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCa                                                                                            <  1:1060659/100‑1 (MQ=255)
       ttCAGAGGAAGATTACCACAGGAAAGCGGGATAAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGc                                                                                             >  1:3741583/1‑99 (MQ=255)
        tCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCAc                                                                                           >  1:800857/1‑100 (MQ=255)
           gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                        >  1:386676/1‑100 (MQ=255)
            agGAAGATTACCACAGGAGAGCGTGATGAAATGGCGGGGGAATGAGCGGTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGGGCAGCTGGCACGGc                                                                                        <  2:2491587/99‑1 (MQ=255)
            agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCa                                                                                       <  1:630239/100‑1 (MQ=255)
            agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCa                                                                                       <  2:2147656/100‑1 (MQ=255)
            agGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATCAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCa                                                                                       <  2:2516486/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTTAATGCATAGATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                      <  2:3232557/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCaa                                                                                      >  1:2956505/1‑100 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                      <  1:3298281/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                      <  1:3727024/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                      <  1:1981542/100‑1 (MQ=255)
             ggAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCaa                                                                                      <  2:3621087/100‑1 (MQ=255)
              gAAGATTACCACAGGAAAGTGGGATGAAATGGCGGAGGAATGAGCGGTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAg                                                                                     <  2:3017045/100‑1 (MQ=255)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAg                                                                                     <  1:2743075/100‑1 (MQ=255)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAg                                                                                     <  1:3627151/100‑1 (MQ=255)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                     <  2:1140621/100‑1 (MQ=39)
              gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAg                                                                                     <  2:31282/100‑1 (MQ=39)
               aaGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAga                                                                                    <  2:2029865/100‑1 (MQ=255)
                aGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgat                                                                                   <  1:2042129/100‑1 (MQ=255)
                   ttACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatgat                                                                                <  1:1875026/100‑1 (MQ=255)
                    tACCACAGGAAAGCGGGATGAAATGGCGGAGTAATGTGCGTTCAAGGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGatgatg                                                                               <  2:2873820/100‑1 (MQ=255)
                    tACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGatgatg                                                                               >  1:2094570/1‑100 (MQ=255)
                     aCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAtgatga                                                                              <  2:259812/100‑1 (MQ=255)
                     aCCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAtgatga                                                                              >  2:875541/1‑100 (MQ=255)
                      ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGaa                                                                             >  1:3849706/1‑100 (MQ=255)
                       cacaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAAc                                                                            <  1:2048302/100‑1 (MQ=255)
                       cacaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAAc                                                                            >  2:563849/1‑100 (MQ=255)
                       cacaGGAAAGCGGGAAGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAAc                                                                            <  2:3551136/100‑1 (MQ=255)
                        acaGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACa                                                                           >  1:1765095/1‑100 (MQ=255)
                              aaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                     <  1:703229/100‑1 (MQ=255)
                                gCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATc                                                                   >  1:2885925/1‑100 (MQ=255)
                                  gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAg                                                                 <  2:2952476/100‑1 (MQ=255)
                                  gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAg                                                                 >  2:3627650/1‑100 (MQ=255)
                                  gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAg                                                                 <  2:3741555/100‑1 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                >  2:2214694/1‑100 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                >  2:3336826/1‑100 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                >  2:3843670/1‑100 (MQ=255)
                                   ggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGt                                                                >  1:944833/1‑100 (MQ=255)
                                       gAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGGTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCa                                                             >  2:3651220/1‑99 (MQ=255)
                                        aaaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGc                                                           >  1:1922855/1‑100 (MQ=255)
                                         aaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCt                                                          <  1:3030722/100‑1 (MQ=255)
                                           tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAACTCAGTCCAGCTGt                                                        >  2:594859/1‑100 (MQ=255)
                                           tGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCCGTCCAGCTGt                                                        >  2:595017/1‑100 (MQ=255)
                                            ggCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                       <  1:2214706/100‑1 (MQ=255)
                                             gCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                      <  1:3493945/100‑1 (MQ=255)
                                             gCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                      >  2:3904723/1‑100 (MQ=255)
                                              cGGAGGAATGAGCGTTCAATGAATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCGGCTGTaca                                                     >  2:2746080/1‑100 (MQ=255)
                                               ggaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTacac                                                    <  2:2681209/100‑1 (MQ=255)
                                                gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                   <  1:121218/100‑1 (MQ=255)
                                                gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                   <  1:3807371/100‑1 (MQ=255)
                                                gaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                   >  1:3569204/1‑100 (MQ=255)
                                                 aggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAccc                                                  <  2:474644/100‑1 (MQ=255)
                                                  ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                 >  1:757888/1‑100 (MQ=255)
                                                   gaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAg                                                >  1:1948457/1‑100 (MQ=255)
                                                    aaTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGGTGATGAACAGAAAAATCAGTCCAGCTGTACACCCAgg                                               >  2:3865700/1‑100 (MQ=255)
                                                     aTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAggc                                              <  2:3619402/100‑1 (MQ=255)
                                                         gCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGgcggcg                                          <  1:2594683/100‑1 (MQ=255)
                                                           gTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                        <  1:3637574/100‑1 (MQ=255)
                                                             tCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggc                                       <  1:102655/99‑1 (MQ=255)
                                                               aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGAc                                    <  1:3524698/100‑1 (MQ=255)
                                                                aTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACg                                   <  1:518989/100‑1 (MQ=255)
                                                                aTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATAAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACg                                   <  1:459580/100‑1 (MQ=255)
                                                                 tGCATCTATTCCTAGAGCGGTTTTCTTGCCGTTTCAGCTCCACGACAAGATGATGAACAGAAAAATCAATCCTGCTGTACATCCAGGCGGCGGGAGACgg                                  <  2:3522052/100‑1 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                  >  2:3498656/1‑100 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                  <  1:2320370/100‑1 (MQ=255)
                                                                 tGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                  <  1:2533824/100‑1 (MQ=255)
                                                                   cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCt                                >  2:1875633/1‑100 (MQ=255)
                                                                     tatatTCCGAGAGCGGTTGTTTTGCCGTGCGAGCTGCACGGGCAGATGGTGAGCAGGAAAATCAGTCCAGCTGGACACCCAGGCGGCGGGGGa                                     >  2:2706536/1‑93 (MQ=255)
                                                                       tatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCAt                            >  1:1531821/1‑100 (MQ=255)
                                                                        atTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                           >  1:3237342/1‑100 (MQ=255)
                                                                         ttCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAACATCAGTCCAGCTGTACACCCAGTCGGCGGGCGACGGCTTCATAg                          <  2:1370387/100‑1 (MQ=255)
                                                                          tCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAgg                         <  1:1382253/100‑1 (MQ=255)
                                                                          tCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGACGAACAGAATAATCAGTCCAGCGGTACACCCAGGCGGCGGGCGACGCCTTCATa                           <  2:2963702/98‑1 (MQ=255)
                                                                           ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCGGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAgg                         <  1:2646581/99‑1 (MQ=255)
                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                     <  1:1646796/100‑1 (MQ=255)
                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                     >  2:1386268/1‑100 (MQ=255)
                                                                              agagCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTc                     <  2:2257296/100‑1 (MQ=255)
                                                                                 gCGGTTGTCTTGCCGTGCCAGCTGCACGGCACGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAt                  <  2:1886328/100‑1 (MQ=255)
                                                                                 gCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGAAGAAAAAAAAAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCg                    <  2:1224459/98‑1 (MQ=255)
                                                                                  cGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATc                 <  1:1715934/100‑1 (MQ=255)
                                                                                  cGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATAAGTGCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGa                   >  1:2497795/1‑98 (MQ=255)
                                                                                     ttGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAgg              <  1:1135492/100‑1 (MQ=255)
                                                                                     ttGTCTTGCCGTGCCAGCTGCACGGCAACATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGATTCGATCAgg              <  2:3294573/100‑1 (MQ=255)
                                                                                       gTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGcc            >  2:1022039/1‑100 (MQ=255)
                                                                                       atCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGcc            >  1:2100365/2‑100 (MQ=39)
                                                                                       atCTTGCCGTGCAGCTGGCACCGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCAAGCGACGGCTTCATAGGCTTCGATCAgg              >  2:1565932/2‑98 (MQ=38)
                                                                                        tCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCa           >  1:306725/1‑100 (MQ=255)
                                                                                        tCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCa           >  1:430586/1‑100 (MQ=39)
                                                                                         cTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAc          >  1:99691/1‑100 (MQ=255)
                                                                                          ttGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCa           >  2:2243788/1‑98 (MQ=255)
                                                                                          ttGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAcc         >  2:1068744/1‑100 (MQ=255)
                                                                                          ttGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAcc         >  1:2838711/1‑100 (MQ=255)
                                                                                               gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGc    >  1:153128/1‑100 (MQ=255)
                                                                                               gTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGc    <  2:2002743/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
TAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTC  >  NC_000913/2596775‑2596971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: