Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,302 Δ1 bp 18.4% coding (221/1773 nt) aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,3010G.18.4% 442.7 / 101.5 152coding (222/1773 nt)aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (57/67);  new base . (21/7);  total (78/74)
Fisher's exact test for biased strand distribution p-value = 6.40e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01

Only 100 of 154 total aligned reads displayed.

AACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTC  >  NC_000913/1950202‑1950399
                                                                                                    |                                                                                                  
aaCATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACg                                                                                                    <  1:3549053/100‑1 (MQ=255)
     cTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGcc                                                                                               >  1:1824408/1‑100 (MQ=255)
      tGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCACA‑CGTACGGTGccc                                                                                              <  1:514527/100‑1 (MQ=255)
       gcgcGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCg                                                                                             <  2:2904908/100‑1 (MQ=255)
         gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                           <  2:2903707/100‑1 (MQ=255)
         gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                           <  1:1517415/100‑1 (MQ=255)
         gcgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                           >  1:1037867/1‑100 (MQ=255)
          cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCCCGCGCA‑CGTACGGTGCCCGTGa                                                                                          >  1:3098593/1‑100 (MQ=255)
          cgGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGa                                                                                          <  2:721523/100‑1 (MQ=255)
           ggTTGATGATAGTCAGCGAGGCCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGGTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                         <  2:1760657/100‑1 (MQ=255)
           ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                         <  1:1904447/100‑1 (MQ=38)
           ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                         <  1:1933561/100‑1 (MQ=38)
           ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAc                                                                                         <  1:3255183/100‑1 (MQ=38)
           ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTg                                                                                           <  2:2531808/98‑1 (MQ=255)
           ggTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGAc                                                                                         <  2:596708/100‑1 (MQ=255)
           ggTTGATGAGAGTCAGCGAGGACGCCAGCACTTCGTTTTCGCCGGTCGCCATGTCGCGGTTCATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGan                                                                                         <  2:3250189/100‑2 (MQ=25)
              tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTg                                                                                      >  2:2096669/1‑100 (MQ=255)
              tgatgaTAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTg                                                                                      <  1:2491714/100‑1 (MQ=255)
               gatgatAGTCAGCGAGGACGTCCGCACTTCGATTTTGCCGGTCGCCATAGCGCGGTTAATATTTTTTTCGTCACGGGCACCGTAC‑GTGCGCGTGAcgaa                                                                                      <  2:2545776/99‑4 (MQ=25)
               gatgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGa                                                                                     >  1:3239512/1‑100 (MQ=255)
                atgatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGaa                                                                                    >  1:1045292/1‑100 (MQ=255)
                atgatAGTCAACGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGTGGTTAATATTTTTGTCGTCACGCGCA‑CGTACGGTGCTCGTGACCTGaa                                                                                    <  2:860050/100‑1 (MQ=255)
                  gatAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATg                                                                                  >  1:2525829/1‑100 (MQ=255)
                    tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTTTTCTTTCTCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                                >  2:1854297/1‑100 (MQ=255)
                    tAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCa                                                                                <  1:2027081/100‑1 (MQ=255)
                      gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                              <  1:2993037/100‑1 (MQ=255)
                      gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                              <  2:473431/100‑1 (MQ=255)
                      gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                              >  2:3752629/1‑100 (MQ=255)
                      gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                              <  1:2922119/100‑1 (MQ=255)
                      gTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGa                                                                              >  2:2454837/1‑100 (MQ=255)
                        cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            <  2:2691568/100‑1 (MQ=255)
                        cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            <  2:3629495/100‑1 (MQ=255)
                        cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            <  1:2581488/100‑1 (MQ=255)
                        cAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAAc                                                                            >  1:3472413/1‑100 (MQ=255)
                          gCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTc                                                                          <  2:1578915/100‑1 (MQ=255)
                           cGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCa                                                                         <  1:1358589/100‑1 (MQ=255)
                            gAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAt                                                                        <  2:2035290/100‑1 (MQ=255)
                            gAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCAt                                                                        <  2:366225/100‑1 (MQ=255)
                                aCGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGGA‑CGTACGGTGCCTGTGTCCTGAATGGAGAACTCATTACg                                                                    <  2:1012886/100‑1 (MQ=255)
                                   ccAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAg                                                                 >  2:3116657/1‑100 (MQ=255)
                                    cAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGt                                                                <  1:2637659/100‑1 (MQ=255)
                                           tCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCagag                                                          <  2:3093620/99‑1 (MQ=255)
                                             gATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGcc                                                       <  2:2009232/100‑1 (MQ=255)
                                              aTTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGGGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCa                                                      >  1:3762086/1‑100 (MQ=255)
                                               tttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAg                                                     >  1:2401693/1‑100 (MQ=255)
                                                ttCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGc                                                    <  2:1113522/100‑1 (MQ=255)
                                                      ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                              <  2:3368156/100‑1 (MQ=255)
                                                      ggTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                              >  1:3332842/1‑100 (MQ=255)
                                                        tCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGACCTCATTACGCAGTTCAGAGGCCAGCTTTAAcg                                             <  2:644389/99‑1 (MQ=255)
                                                        tCGCCATATCGCGGTTAATATTTTTTACGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGATCTCATTACGCAGTTCAGAGGCCAGCTTTAAc                                              <  2:1535272/98‑1 (MQ=255)
                                                         cGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAAcgcg                                           >  1:1120353/1‑100 (MQ=255)
                                                          gCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGGATGCAGAAATCATTACGAAGTTCCGAGGCCAGCTTTAAcgag                                           >  2:3272916/1‑97 (MQ=255)
                                                          gCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGt                                          >  1:199924/1‑100 (MQ=255)
                                                             atatCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCg                                       <  2:682535/100‑1 (MQ=255)
                                                               atCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCa                                     <  1:2698016/100‑1 (MQ=255)
                                                               atCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCa                                     <  1:2096680/100‑1 (MQ=255)
                                                                tCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCAc                                    >  1:3724392/1‑100 (MQ=255)
                                                                 cgcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACg                                   <  2:1037861/100‑1 (MQ=255)
                                                                  gcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGa                                  >  1:801275/1‑100 (MQ=255)
                                                                  gcgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGa                                  >  1:861467/1‑100 (MQ=255)
                                                                   cgGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGAt                                 <  1:2794422/100‑1 (MQ=255)
                                                                   cgGTTAATATTTTTTCCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATCCAGAACTCATTACGGAGTTCAGAGGCCAGCTTTAACACGTCAGCACGa                                  >  2:3632530/1‑99 (MQ=255)
                                                                    ggTTAATATTTTTTTCGTCCCGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGGGGCCAGCTTTAACGCGTCCCCACGacc                                >  2:955989/1‑98 (MQ=255)
                                                                       tAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATTCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATc                                >  1:2268514/1‑97 (MQ=255)
                                                                       tAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCgg                             <  2:1411997/100‑1 (MQ=255)
                                                                         atatTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGAt                           >  1:1237901/1‑100 (MQ=255)
                                                                           atTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCg                         <  1:187308/100‑1 (MQ=255)
                                                                            tttttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGa                        <  2:2306498/100‑1 (MQ=255)
                                                                            tttttttCGCCACGCGCA‑CTTCCGGTGCCCGTGCCCTGAATGCATAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                          <  2:445254/98‑1 (MQ=255)
                                                                            tttgtttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAGCTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATc                          <  2:847513/98‑1 (MQ=255)
                                                                             ttttttCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaa                       <  1:3014589/100‑1 (MQ=255)
                                                                                 caggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAAt                   >  1:3809854/4‑100 (MQ=37)
                                                                                  aggccACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCCGTTCAGAGGCCAGCTGTAACGCGTCCGCACGATCCGGATCGAAAAATa                  >  1:1776018/5‑100 (MQ=37)
                                                                                  aggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATa                  >  1:208612/3‑100 (MQ=37)
                                                                                   ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  2:3284177/2‑100 (MQ=38)
                                                                                   ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  2:1974390/2‑100 (MQ=38)
                                                                                   ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  2:1341458/2‑100 (MQ=38)
                                                                                   ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCAGATCggaagagcg                 >  2:2697374/2‑92 (MQ=25)
                                                                                   ggTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAACTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 >  2:1651360/2‑100 (MQ=37)
                                                                                   cGTCACGCGGA‑CGTACGGTGCCCGTGTCCTGAGTGCAGAACTCATTTCGCAGTCCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAc                 <  2:1487070/100‑1 (MQ=255)
                                                                                   acccACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGACCTCATTACGCAGTTCCCAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGaaaa                     >  2:229978/4‑96 (MQ=25)
                                                                                    gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                >  2:1539925/1‑100 (MQ=38)
                                                                                    gTCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                >  2:3824346/1‑100 (MQ=38)
                                                                                    gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                <  1:519119/100‑1 (MQ=255)
                                                                                    gTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTGTAACGCGTCCGCACGATCCGGATCGAAAAATAcc                >  2:2219976/1‑100 (MQ=255)
                                                                                     tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  1:2440437/1‑100 (MQ=38)
                                                                                     tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  1:672363/1‑100 (MQ=38)
                                                                                     tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  1:2738891/1‑100 (MQ=38)
                                                                                     tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  2:3843539/1‑100 (MQ=38)
                                                                                     tCACGGGCACCGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCt               >  2:2208452/1‑100 (MQ=38)
                                                                                      cACGGGCACCGTAC‑GTGCGCGTGACCTGAGTGCAGAACCCATTACGCAGTGCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg              >  1:2288822/1‑100 (MQ=255)
                                                                                      cACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTg              >  1:1204922/1‑100 (MQ=255)
                                                                                        cgggcaccGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa            >  2:2050102/8‑100 (MQ=255)
                                                                                        cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGc             >  2:3773883/1‑99 (MQ=255)
                                                                                        cgcgCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCa            <  1:3929119/100‑1 (MQ=255)
                                                                                          ggcaccGTAC‑GTGCGCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACg          >  1:3162551/6‑100 (MQ=255)
                                                                                          cgcA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACg          >  1:2907628/1‑100 (MQ=255)
                                                                                             a‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATa       <  1:3651326/100‑1 (MQ=255)
                                                                                               cGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATAc      >  1:3399502/1‑100 (MQ=255)
                                                                                                  aCGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCtt   <  1:2048369/100‑1 (MQ=255)
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AACATCTGCGCGGTTGATGATAGTCAGCGAGGACGCCAGCACTTCGATTTCGCCGGTCGCCATATCGCGGTTAATATTTTTTTCGTCACGCGCA‑CGTACGGTGCCCGTGACCTGAATGCAGAACTCATTACGCAGTTCAGAGGCCAGCTTTAACGCGTCCGCACGATCCGGATCGAAAAATACCTGCACGATACCTTC  >  NC_000913/1950202‑1950399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑