Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,607:1 +G 15.0% coding (443/822 nt) yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6071.G15.0% 573.9 / 103.8 160coding (443/822 nt)yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (62/74);  new base G (12/12);  total (74/86)
Fisher's exact test for biased strand distribution p-value = 8.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 162 total aligned reads displayed.

GTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAG  >  NC_000913/2449508‑2449705
                                                                                                    |                                                                                                  
 tCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACt                                                                                                   <  2:1277207/100‑1 (MQ=255)
    aCTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATc                                                                                                <  1:139625/100‑1 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               <  2:916961/100‑1 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               >  1:2233748/1‑100 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               <  2:71031/100‑1 (MQ=255)
     cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCa                                                                                               <  1:1313781/100‑1 (MQ=255)
      ttgaGGGTGAGCTGGTGTGACTTACTGATATAATTCGCAGTCTATGAAACGTCTTAAGGTTGAACAGAAACGGTTGGTCAAATCGTGTCGTGACTATCAg                                                                                              <  2:3396443/97‑1 (MQ=255)
       gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAgg                                                                                             >  1:100897/1‑100 (MQ=255)
       gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAgg                                                                                             >  2:416762/1‑100 (MQ=255)
         aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcc                                                                                           >  2:2678154/1‑100 (MQ=255)
           ggTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcccc                                                                                         <  1:901430/100‑1 (MQ=255)
             tGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTg                                                                                       <  2:123847/100‑1 (MQ=255)
              gAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGa                                                                                      <  1:3348116/100‑1 (MQ=255)
              gAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGa                                                                                      <  2:232881/100‑1 (MQ=255)
               aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGAt                                                                                     <  1:1771475/100‑1 (MQ=255)
                gCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATa                                                                                    >  1:2203173/1‑100 (MQ=255)
                 cAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATAc                                                                                   <  1:3723939/100‑1 (MQ=255)
                  aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATa                                                                                    <  1:1497804/98‑1 (MQ=255)
                     tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCa                                                                               <  1:1528732/100‑1 (MQ=255)
                      ttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCAc                                                                              >  2:236923/1‑100 (MQ=255)
                       tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACg                                                                             <  2:2500783/100‑1 (MQ=38)
                       tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACg                                                                             <  1:2547498/100‑1 (MQ=38)
                       tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACg                                                                             <  2:395467/100‑1 (MQ=38)
                       tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                             <  1:3517187/100‑1 (MQ=255)
                        gACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGc                                                                            <  1:2678171/100‑1 (MQ=255)
                        gACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGc                                                                            >  2:2943417/1‑100 (MQ=255)
                         aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGTATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgac                                                                           <  2:1756521/100‑3 (MQ=37)
                         aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgac                                                                           <  2:2616484/100‑3 (MQ=37)
                          cTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                          <  1:2853232/100‑4 (MQ=37)
                           ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                         <  1:6712/100‑5 (MQ=25)
                              cTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg                                                                      >  1:780538/1‑100 (MQ=255)
                               tGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTgg                                                                     <  1:1204780/100‑1 (MQ=255)
                                gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                    >  1:993415/1‑100 (MQ=255)
                                gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                    >  1:1239652/1‑100 (MQ=255)
                                 aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgag                                                                   <  1:1969159/100‑1 (MQ=255)
                                  cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                  >  2:837083/1‑100 (MQ=255)
                                  cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                  >  2:591113/1‑100 (MQ=255)
                                  cATAATTCTCATGCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                  >  1:2602716/1‑100 (MQ=255)
                                   aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                 <  2:1667373/100‑1 (MQ=255)
                                   aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                 <  1:1654804/100‑1 (MQ=255)
                                    tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAgg                                                                >  1:2881204/1‑100 (MQ=255)
                                     aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGc                                                               >  2:344106/1‑100 (MQ=255)
                                     aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGc                                                               <  1:3461728/100‑1 (MQ=255)
                                     aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGc                                                               <  1:281132/100‑1 (MQ=255)
                                       ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                             >  1:767145/1‑100 (MQ=255)
                                         ctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                             >  2:113553/1‑98 (MQ=255)
                                            aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGCGAGGCTTCagag                                                        >  1:1139014/1‑100 (MQ=255)
                                             ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                       >  1:2048234/1‑100 (MQ=255)
                                               ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                     >  1:2513922/1‑100 (MQ=255)
                                                 aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGCGGCTTCAGAGtcatt                                                   >  1:1459038/1‑100 (MQ=255)
                                                  tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATAAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattc                                                  >  2:378735/1‑100 (MQ=255)
                                                      aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                              >  1:1159302/1‑100 (MQ=255)
                                                       cGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                              <  2:416019/99‑1 (MQ=255)
                                                        gTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                            <  1:3544301/100‑1 (MQ=255)
                                                         tCATAAGGTTGAACAGAAATGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCAtaataa                                           >  2:2399574/1‑100 (MQ=255)
                                                         tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTTACGCCCCCTGGAGAGGCTTCACAGTCATTCataata                                            >  1:3880160/1‑99 (MQ=255)
                                                          cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaac                                          <  1:790392/100‑1 (MQ=255)
                                                          cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGGCATTCATAATaac                                          <  2:2443236/100‑1 (MQ=255)
                                                           aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                         <  2:1045733/100‑1 (MQ=255)
                                                                gTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAaccaacca                                    >  1:3903357/1‑100 (MQ=255)
                                                                  tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                  >  2:3061869/1‑100 (MQ=255)
                                                                  tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCACCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                  <  2:2161353/100‑1 (MQ=255)
                                                                   gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATgg                                 >  1:928839/1‑100 (MQ=255)
                                                                   gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATgg                                 <  1:508995/100‑1 (MQ=255)
                                                                   gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATgg                                 <  2:3880130/100‑1 (MQ=255)
                                                                    aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGa                                <  1:186034/100‑1 (MQ=255)
                                                                    aaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTGCAGAGTCATTCATAATAACCCACCATGGa                                >  2:1963663/1‑100 (MQ=255)
                                                                    aaCAGAAACGGATGGACAAATCGTGACGTGACTAGCAGGCCCCTGATACGTGACGCCCCCTGGAGAGGCTTCAGCGTCATTAATAATAACTAGCCCTGGa                                >  1:662334/1‑100 (MQ=255)
                                                                      cAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACa                              <  1:2154373/100‑1 (MQ=255)
                                                                        gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                            >  1:2938214/1‑100 (MQ=255)
                                                                          aaCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGa                           <  2:3809782/99‑1 (MQ=255)
                                                                            cGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaaa                        >  1:1733855/1‑100 (MQ=255)
                                                                            cGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaaaa                        >  1:3269535/1‑100 (MQ=255)
                                                                             ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                       <  2:1094206/100‑1 (MQ=255)
                                                                             ggATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                       <  1:922304/100‑1 (MQ=255)
                                                                                 ggACAAATCGTGTCGTTACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAg                   <  2:1534791/100‑1 (MQ=255)
                                                                                  gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCGTTCATAATCACCAACCATGGACACAAAAAGTagaa                  >  2:3528040/1‑97 (MQ=255)
                                                                                    cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                <  1:1337307/100‑1 (MQ=255)
                                                                                      aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa              >  1:102483/1‑100 (MQ=255)
                                                                                      aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa              <  2:2582891/100‑1 (MQ=255)
                                                                                         cgtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc           <  2:1257433/100‑1 (MQ=255)
                                                                                         cgtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc           <  1:236923/100‑1 (MQ=255)
                                                                                          gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtct          >  1:2872612/1‑100 (MQ=255)
                                                                                          ggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtct          >  1:311525/5‑100 (MQ=25)
                                                                                            gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        <  2:100897/100‑1 (MQ=255)
                                                                                            gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  2:3517687/1‑100 (MQ=255)
                                                                                            ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:3115337/3‑100 (MQ=37)
                                                                                            ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:767622/3‑100 (MQ=37)
                                                                                            ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:3613679/3‑100 (MQ=37)
                                                                                            ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct        >  1:3029200/3‑100 (MQ=37)
                                                                                             tcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg       <  2:2313486/100‑1 (MQ=255)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  1:3622871/1‑100 (MQ=38)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  1:3479491/1‑100 (MQ=38)
                                                                                              cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  2:458449/1‑100 (MQ=38)
                                                                                              cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      >  2:322933/1‑100 (MQ=255)
                                                                                              cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc      <  1:1233138/100‑1 (MQ=255)
                                                                                               gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa     <  1:2390232/100‑1 (MQ=255)
                                                                                               gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa     >  1:1746097/1‑100 (MQ=255)
                                                                                                tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag    <  2:3818553/100‑1 (MQ=255)
                                                                                                 gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga   >  1:252097/1‑100 (MQ=255)
                                                                                                    |                                                                                                  
GTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAG  >  NC_000913/2449508‑2449705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: