New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4417460138 (0.980)8 (0.060) 6/164 NT 5.7% coding (509/750 nt) yjfP acyl CoA esterase
?NC_000913 = 4417479 137 (1.050)coding (528/750 nt) yjfP acyl CoA esterase
Rejected: Frequency below cutoff threshold.

CCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4417355‑4417460
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgtcgcTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATGT  <  NC_000913/4417479‑4417381
                                                                                                                                                                                                             
CCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACAT                                                                                                           <  2:216494/100‑1
  AGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTG                                                                                                         <  1:810903/100‑1
  AGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTG                                                                                                         >  1:2525522/1‑100
   GCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGT                                                                                                        >  2:609473/1‑100
    CATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAACAGGCAGCACAGCAGAATGAATTGCATAACATAGTC                                                                                                       >  1:1706045/1‑100
      ttccgatcTCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGC                                                                                                     <  2:932333/92‑1
      TGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATCACATTGTCGC                                                                                                     >  2:1565975/1‑100
               CGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCagatcggaa                                                                                            >  1:932338/1‑91
                              CACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGAC                                                                             <  1:1123874/100‑1
                                CTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAA                                                                           <  1:1936510/100‑1
                                  CGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATG                                                                         <  2:2539616/100‑1
                                  CGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATG                                                                         <  2:2180527/100‑1
                                     CCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTA                                                                      <  1:1370857/100‑1
                                      CGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTAT                                                                     <  1:1600694/100‑1
                                       GTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATT                                                                    <  1:1755436/100‑1
                                       GTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATT                                                                    <  2:1555375/100‑1
                                                                                                   TTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAG        <  1:3229447/100‑1
                                                                                                   TTGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAG        <  1:184244/100‑1
                                                                                                    TGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGT       <  2:128936/100‑1
                                                                                                    TGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGT       <  1:1125514/100‑1
                                                                                                    TGTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGT       >  2:243473/1‑100
                                                                                                     GTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTG      >  1:770045/1‑100
                                                                                                     GTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTG      >  1:3634461/1‑100
                                                                                                     GTCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCGGCCGTTTCAGGAATCAGCGGTGGAAACAGTGAACGGGCGAGT       >  2:1374925/1‑99
                                                                                                      TCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGA     <  1:2025465/100‑1
                                                                                                      TCGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGA     <  2:1288926/100‑1
                                                                                                       CGCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGAT    <  2:851072/100‑1
                                                                                                       CGCTTCCCACACTGCCAGTGGCTCGACAATGTTATTGAATTGATTCTGCTGTGCGGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGAT    <  2:704091/100‑1
                                                                                                        GCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATG   <  1:888478/100‑1
                                                                                                        GCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATG   <  1:510540/100‑1
                                                                                                        GCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATG   >  1:3654120/1‑100
                                                                                                        GCTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATG   <  1:2703650/100‑1
                                                                                                         CTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATGT  >  1:1001973/1‑100
                                                                                                         CTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATG   <  2:950816/99‑1
                                                                                                                                                                                                             
CCAGCATGATGGGATCGGGCTATTTTACATCACTCGCCCGTTCACTGTTTCCACCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4417355‑4417460
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgtcgcTTCCCACTCTGCCAGTGGCGCGACAATGTTATTGAATTCATTCTGCTGTGCTGCCGTTTCAGGTATCAGCGGTGGAAACAGTGAACGGGCGAGTGATGT  <  NC_000913/4417479‑4417381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.