Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,653,372 T→C 18.1% intergenic (+33/+785) sseA → / ← sseB 3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,653,3720TC18.1% 371.2 / 78.7 171intergenic (+33/+785)sseA/sseB3‑mercaptopyruvate sulfurtransferase/rhodanase‑like enzyme, sulfur transfer from thiosulfate
Reads supporting (aligned to +/- strand):  ref base T (61/79);  new base C (21/10);  total (82/89)
Fisher's exact test for biased strand distribution p-value = 1.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-01

Only 100 of 174 total aligned reads displayed.

CTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACC  >  NC_000913/2653274‑2653470
                                                                                                  |                                                                                                  
cTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTg                                                                                                   <  1:3795063/100‑1 (MQ=255)
  gTACGACGGCGCATGGAGTGAATGGGGCGCTCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGc                                                                                                 <  2:3221156/100‑1 (MQ=255)
  gTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGc                                                                                                 >  1:1684853/1‑100 (MQ=255)
    acgacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCgg                                                                                               <  1:215814/100‑1 (MQ=255)
    acgacgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAACAAAAAACCGCCGAATTTGGCgg                                                                                               <  2:2288591/100‑1 (MQ=255)
        cgGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttt                                                                                           <  1:544777/100‑1 (MQ=255)
         ggCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAGCCGCCAAATTCGGCGGttttt                                                                                          <  1:1667534/100‑1 (MQ=255)
         ggCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGtttt                                                                                           <  2:1614806/99‑1 (MQ=255)
          gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttttt                                                                                         <  1:1049902/100‑1 (MQ=255)
          gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttttt                                                                                         <  2:804223/100‑1 (MQ=255)
          gcgcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGtttt                                                                                           <  2:3178669/98‑1 (MQ=255)
            gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAt                                                                                       >  2:1285179/1‑100 (MQ=255)
            gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTAt                                                                                       <  1:3364336/100‑1 (MQ=255)
            gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTAt                                                                                       <  2:2699841/100‑1 (MQ=255)
            gcATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTAt                                                                                       <  1:2450555/100‑1 (MQ=255)
             cATGGAGTGAATGGGGCGCGCTGGTAGATTTACCTGTTGAGCCAGTGAAATAAGTATTTTATATTCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAt                                                                                       <  2:3766956/99‑1 (MQ=255)
             cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAtt                                                                                      >  1:1877822/1‑100 (MQ=255)
             cATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTAtt                                                                                      <  1:706437/100‑1 (MQ=255)
              aTGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTg                                                                                     <  1:1345193/100‑1 (MQ=255)
              aTGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTg                                                                                     <  1:2203988/100‑1 (MQ=255)
               tGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGc                                                                                    >  2:1243063/1‑100 (MQ=255)
               tGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGc                                                                                    <  2:3916905/100‑1 (MQ=255)
               tGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCAAATTCGGCGGTTTTTTATTGc                                                                                    <  1:2434624/100‑1 (MQ=255)
                ggAGGGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCt                                                                                   <  2:1824567/100‑1 (MQ=255)
                  aGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAg                                                                                 >  2:3320153/1‑100 (MQ=255)
                     gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCt                                                                              >  1:3639416/1‑100 (MQ=255)
                     gAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCt                                                                              >  2:46553/1‑100 (MQ=255)
                       aTGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTgg                                                                            <  1:3168642/100‑1 (MQ=255)
                        tGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGt                                                                           <  1:240769/100‑1 (MQ=255)
                        tGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGt                                                                           <  2:58936/100‑1 (MQ=255)
                            gcgcgcGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgc                                                                       >  2:3633191/1‑100 (MQ=255)
                             cgcgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTcgcg                                                                      <  1:2752444/100‑1 (MQ=255)
                               cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGc                                                                    <  1:153639/100‑1 (MQ=255)
                               cgcgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGc                                                                    >  1:1718366/1‑100 (MQ=255)
                                 cgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGTTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                  <  2:1586077/100‑1 (MQ=255)
                                 cgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                  <  1:2850677/100‑1 (MQ=255)
                                 cgGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCt                                                                  >  1:3637406/1‑100 (MQ=255)
                                  gggCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCtt                                                                 <  1:1939892/100‑1 (MQ=255)
                                   ggCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCttt                                                                >  2:3498667/1‑100 (MQ=255)
                                       gATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcag                                                            <  1:1483174/100‑1 (MQ=255)
                                          ttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagcag                                                         <  2:2617645/100‑1 (MQ=255)
                                          ttACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCcagcag                                                         >  1:3603341/1‑100 (MQ=255)
                                           tACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAgg                                                        <  1:3309770/100‑1 (MQ=255)
                                           tACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAgg                                                        <  1:1372898/100‑1 (MQ=255)
                                            aCCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGt                                                       <  2:2499627/100‑1 (MQ=255)
                                            aCCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGt                                                       <  2:2993516/100‑1 (MQ=255)
                                              cGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTg                                                     <  2:170967/100‑1 (MQ=255)
                                               ggTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGa                                                    >  1:495022/1‑100 (MQ=255)
                                                gTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGAc                                                   <  1:1757323/100‑1 (MQ=255)
                                                 ttGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGAc                                                   <  2:1464088/99‑1 (MQ=255)
                                                   gAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtg                                                >  2:306618/1‑100 (MQ=255)
                                                    aGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTtgt                                               >  1:3444741/1‑100 (MQ=255)
                                                      ccAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTgtgt                                             <  2:1700802/100‑1 (MQ=255)
                                                       cAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGtt                                            <  1:2794370/100‑1 (MQ=255)
                                                        aGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTa                                           <  1:2058043/100‑1 (MQ=255)
                                                           gAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                         <  1:3736299/99‑1 (MQ=255)
                                                           gAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACAt                                        <  1:1550001/100‑1 (MQ=255)
                                                           gAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACAt                                        <  2:3727916/100‑1 (MQ=255)
                                                           gAAATAAGTAGTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACa                                         <  1:6771/99‑1 (MQ=255)
                                                            aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATg                                       <  2:1579766/100‑1 (MQ=255)
                                                            aaaTAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATg                                       <  1:2216316/100‑1 (MQ=255)
                                                                aaGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCa                                   <  2:193831/100‑1 (MQ=255)
                                                                 aGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCaa                                  >  1:784713/1‑100 (MQ=255)
                                                                  gTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAAc                                 <  1:3467159/100‑1 (MQ=255)
                                                                  gTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAAc                                 >  1:1341168/1‑100 (MQ=255)
                                                                     ttttACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGTCTTGTGTTACATGAGCAACGCa                              <  2:2284453/100‑1 (MQ=255)
                                                                     ccgatctggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCa                              <  2:1690113/93‑1 (MQ=255)
                                                                            ggCAATAAAAAACCGCCGAATTTGGCGGTTTTTTGTTGCTAGTCTGGTTCGCGGCCTGTCGTGAATGTTGATTCGTGTTACATGATCGATTCAGGTGCtt                       >  2:2023370/1‑100 (MQ=21)
                                                                             gCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTc                      <  2:872120/100‑1 (MQ=255)
                                                                             ccgatctaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTc                      <  2:2817955/93‑1 (MQ=255)
                                                                               aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac                    <  1:591871/100‑1 (MQ=255)
                                                                               aaTAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac                    >  1:2919294/1‑100 (MQ=255)
                                                                               aaTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcac                    >  1:1958512/1‑100 (MQ=255)
                                                                                ntAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                   >  2:3580695/2‑100 (MQ=255)
                                                                                aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                   >  1:3622731/1‑100 (MQ=255)
                                                                                aTAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcaca                   >  1:966221/1‑100 (MQ=255)
                                                                                 tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTTTGTTACATGAGCAACGCAGGTGCTTCACAg                  >  2:153996/1‑100 (MQ=255)
                                                                                 tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg                  <  1:413502/100‑1 (MQ=255)
                                                                                 tAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGGCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg                  <  2:2058163/100‑1 (MQ=255)
                                                                                 tAAAAAACCGCCAAATTCGGCGGTTTTTTATTGCCAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAg                  >  1:2720820/1‑100 (MQ=37)
                                                                                  aaaaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGc                 <  2:3296005/100‑1 (MQ=255)
                                                                                   aaaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCa                >  1:3704465/1‑100 (MQ=255)
                                                                                   aaaaaCCGCCAAATTCGGCGGTTTTTTATTGCCTGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCa                >  1:3814733/1‑100 (MQ=37)
                                                                                    aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTcagatcgg               >  1:2817973/1‑94 (MQ=255)
                                                                                    aaaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaa               >  1:648179/1‑100 (MQ=255)
                                                                                    aaaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCCCAg                  >  2:3776594/1‑97 (MQ=37)
                                                                                     aaaCCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACACAGGTGCTTCACAGCaaa              >  1:3197341/1‑100 (MQ=255)
                                                                                     aaaCCGCCGAATTTGACGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa              <  1:3891357/100‑1 (MQ=255)
                                                                                     aaaCCGCCAAATTCGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCaaa              >  1:2913869/1‑100 (MQ=255)
                                                                                        ccgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACa           <  1:2582719/100‑1 (MQ=255)
                                                                                        ccgccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACa           <  1:2996975/100‑1 (MQ=255)
                                                                                          gccgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGAAGCCTTTCCACCAGGTTGACTTGTGTTACATGACCAACTCAGGTTCTTCACAGCCAAACAAt         >  2:2109809/1‑100 (MQ=255)
                                                                                            cgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAc       >  1:2281413/1‑100 (MQ=255)
                                                                                            cgAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATAc       <  2:1153738/100‑1 (MQ=255)
                                                                                             gAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACt      >  2:1041274/1‑100 (MQ=255)
                                                                                              aaTTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTc     <  1:210990/100‑1 (MQ=255)
                                                                                               aTTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCa    >  2:2675401/1‑100 (MQ=255)
                                                                                                 ttGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAcc  <  1:1046239/100‑1 (MQ=255)
                                                                                                 ttGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAcc  <  1:1265720/100‑1 (MQ=255)
                                                                                                  tGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCAcc  <  1:526852/99‑1 (MQ=255)
                                                                                                  |                                                                                                  
CTGTACGACGGCGCATGGAGTGAATGGGGCGCGCGGGCAGATTTACCGGTTGAGCCAGTGAAATAAGTATTTTACAGGCAATAAAAAACCGCCGAATTTGGCGGTTTTTTATTGCTAGTCTGGTTCGCGGCCTTTCCAGCAGGTTGACTTGTGTTACATGAGCAACGCAGGTGCTTCACAGCAAAACAATACTCACC  >  NC_000913/2653274‑2653470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: