Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,296 C→T 16.8% intergenic (‑31/+20) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,2960CT16.8% 374.3 / 58.6 160intergenic (‑31/+20)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (60/73);  new base T (13/14);  total (73/87)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

Only 100 of 163 total aligned reads displayed.

GCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCT  >  NC_000913/3918198‑3918395
                                                                                                  |                                                                                                   
gCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCt                                                                                                    >  2:1841525/1‑100 (MQ=255)
 cTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTa                                                                                                   <  1:383729/100‑1 (MQ=255)
   ttAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAg                                                                                                 <  1:3344378/100‑1 (MQ=255)
         aTCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgc                                                                                           <  2:1787067/100‑1 (MQ=255)
           cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccg                                                                                         >  1:508471/1‑100 (MQ=255)
           cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccg                                                                                         <  2:206482/100‑1 (MQ=255)
            ttttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgc                                                                                        <  1:3635965/100‑1 (MQ=255)
            ttttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgc                                                                                        <  1:3108447/100‑1 (MQ=255)
             tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgca                                                                                       <  2:1169637/100‑2 (MQ=255)
             tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgc                                                                                        <  1:3852793/99‑1 (MQ=255)
             tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTGAGGGCAGgccgca                                                                                       <  1:2650781/100‑2 (MQ=255)
             tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgcc                                                                                       <  2:90372/100‑1 (MQ=255)
              ttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCa                                                                                      <  1:1394444/100‑1 (MQ=255)
               tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                     <  1:1794403/100‑1 (MQ=38)
               tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAg                                                                                     <  2:3780289/100‑1 (MQ=255)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                    <  1:788895/100‑2 (MQ=38)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                    <  2:3224610/100‑2 (MQ=38)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                    <  2:2421725/100‑2 (MQ=38)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGa                                                                                    <  2:3168872/100‑1 (MQ=255)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGa                                                                                    <  2:2570515/100‑1 (MQ=255)
                gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGa                                                                                    <  1:3019690/100‑1 (MQ=255)
                  gtgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                  >  1:1067791/1‑100 (MQ=255)
                  gtgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                  <  2:2050244/100‑1 (MQ=255)
                    gtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGtt                                                                                >  2:1087390/1‑100 (MQ=255)
                      tCTGGACGCTTGCGATCTTACTTCGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCTTTTTCTTGCGGCGTGCCCTCAGGCAAGCAGATAGACGTTAc                                                                              <  2:883188/100‑1 (MQ=255)
                       cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                             >  2:1688700/1‑100 (MQ=255)
                       cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                             >  1:257759/1‑100 (MQ=255)
                         ggACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAg                                                                           >  1:791317/1‑100 (MQ=255)
                         gaaCGCTTGCGATCTTACTACGTACCTCTTTTGCGCCGGCCATGACCTTCTCCTCAATGCCCTGCGCCCTGCCCTAAGGCAAGCCGCCAGACGTTAc                                                                              <  2:2351524/95‑1 (MQ=255)
                                tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTggg                                                                    >  1:798000/1‑100 (MQ=255)
                                  cGATCTTACTACGTATCTCTTTTTCTTTTTTCATTTGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGttt                                                                  >  2:2492755/1‑100 (MQ=255)
                                  cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                  >  1:1953670/1‑100 (MQ=255)
                                  cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                  >  2:698367/1‑100 (MQ=255)
                                  cGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGtt                                                                  <  1:866186/100‑1 (MQ=255)
                                    aTCTTACTACGTATTTCTTTTGCGCCGGCCATGGGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                <  2:1406466/100‑1 (MQ=255)
                                    aTCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGc                                                                >  1:550286/1‑100 (MQ=255)
                                        tactacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTg                                                            <  2:436768/100‑1 (MQ=255)
                                         actacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                           >  1:1530492/1‑100 (MQ=255)
                                          ctacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                          <  1:1087397/100‑1 (MQ=255)
                                          ctacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGaa                                                          >  1:1910616/1‑100 (MQ=255)
                                           tacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                         <  1:226447/100‑1 (MQ=255)
                                           tacGTATCTCTTTTGCGCCGGCCACGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                         <  1:2446989/100‑1 (MQ=255)
                                            acGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                        >  1:196762/1‑100 (MQ=255)
                                             cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                       >  1:54657/1‑100 (MQ=255)
                                             cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                       >  2:1243641/1‑100 (MQ=255)
                                               tATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAt                                                     >  1:2827575/1‑100 (MQ=255)
                                               tATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATGGGGTTGCTTTGAAGGAAt                                                     >  1:2213016/1‑100 (MQ=255)
                                                aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATc                                                    <  1:2304052/100‑1 (MQ=255)
                                                aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATc                                                    >  2:2231808/1‑100 (MQ=255)
                                                  ctctTTTGCGCCGGACATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGa                                                  <  2:1495776/100‑1 (MQ=255)
                                                   tctTTTGCGCCGGCCCTGAGCTTCTCCTCACTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGCTTGCTTTGAAGGAAGCGAg                                                 >  2:3730200/1‑100 (MQ=255)
                                                   tctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTTCGGCCTGCCCTCAGGCAAGCCGCCAGACGTTACCCGGGTTGGGTTGATTTGAAGGCATCGAg                                                 >  2:1434410/1‑100 (MQ=255)
                                                    ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAgg                                                >  2:2961377/1‑100 (MQ=255)
                                                      tttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGCAGGAATCGAggtt                                              <  1:2673371/100‑3 (MQ=255)
                                                      tttGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGAt                                              <  2:161670/100‑1 (MQ=255)
                                                         gcgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                           >  2:2253077/1‑100 (MQ=255)
                                                          cgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGTCGCCAGACGTTACTAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                          >  2:587865/1‑100 (MQ=255)
                                                          cgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                          >  1:3340032/1‑100 (MQ=255)
                                                          cgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGACTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAGGAATCGAGGATGc                                            >  2:307876/1‑98 (MQ=255)
                                                              ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                      <  1:687751/100‑1 (MQ=255)
                                                                ccATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                    <  1:2120339/100‑1 (MQ=255)
                                                                   tGAGCTTCTCCTCAATGCCTTGCGGCCTACCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                 >  1:2726618/1‑100 (MQ=255)
                                                                    gAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGc                                <  1:3251810/100‑1 (MQ=255)
                                                                     aGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGcc                               <  2:504255/100‑1 (MQ=255)
                                                                      gCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCt                              <  1:202619/100‑1 (MQ=255)
                                                                       cTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCtt                             <  2:2445317/100‑1 (MQ=255)
                                                                          ctcctgAATGCCTTTCGACCTTCCCTAAGGAAAGCGGCCAGACGTTACCAGTATTGGGTTGCTTTGAAGGAATCGTGGATGCCTTTCAGCTTGCCTTcn                           <  2:2095154/99‑2 (MQ=255)
                                                                           tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGTCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                         <  1:1243650/100‑1 (MQ=255)
                                                                           tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                         <  1:3012980/100‑1 (MQ=255)
                                                                            cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAtt                        >  1:1639322/1‑100 (MQ=255)
                                                                            cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAtt                        >  1:120788/1‑100 (MQ=255)
                                                                            ccgcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAtt                        <  2:1326236/97‑1 (MQ=255)
                                                                             ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAttt                       >  2:2841112/1‑100 (MQ=255)
                                                                             ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAttt                       >  2:307309/1‑100 (MQ=255)
                                                                              tcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtc                      >  1:3274331/1‑100 (MQ=255)
                                                                               cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                     <  2:3601559/100‑1 (MQ=255)
                                                                               cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                     >  2:2780225/1‑100 (MQ=255)
                                                                                  tGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                  <  1:475615/100‑1 (MQ=255)
                                                                                  tGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcgtcg                  <  1:3426613/100‑1 (MQ=255)
                                                                                    tctggCGGCTTGCCTTAGGGCAGGCCGCAAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                >  1:596316/5‑100 (MQ=37)
                                                                                    ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                >  2:568049/1‑100 (MQ=255)
                                                                                      ttGCGGGCTGCCCTAAGGCAAGCCGACAGACGGTACCAGGCTTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTg               <  2:3346184/99‑1 (MQ=255)
                                                                                       tGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa             >  2:1417601/1‑100 (MQ=255)
                                                                                       tGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa             <  2:508468/100‑1 (MQ=255)
                                                                                       ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa             >  2:840111/2‑100 (MQ=255)
                                                                                       ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa             >  2:127801/2‑100 (MQ=255)
                                                                                       ggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTa             >  1:740661/2‑100 (MQ=255)
                                                                                        gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg            >  1:1897108/1‑100 (MQ=255)
                                                                                        gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCCTGCCTTCGATTTCGTCGTTGTAg            >  1:2552048/1‑100 (MQ=255)
                                                                                         cGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTGGc           >  2:1689873/1‑100 (MQ=255)
                                                                                          ggCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGcc          >  2:2748873/1‑100 (MQ=255)
                                                                                          ggCCTGCCCTAAGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAGTCGAGGCTGCCTTTCAGCTTGCCTTCGATTCCGTCGTTGTCGcc          >  2:1446461/1‑100 (MQ=255)
                                                                                          ggCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGcc          >  1:3664051/1‑100 (MQ=255)
                                                                                          ggCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGcc          <  2:56065/100‑1 (MQ=255)
                                                                                            ccTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAc        <  2:1953661/100‑1 (MQ=255)
                                                                                            ccTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAc        <  2:2322937/100‑1 (MQ=255)
                                                                                              tGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCg      >  2:3829167/1‑100 (MQ=255)
                                                                                               gCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCTGCTTGCCTTCGATTTCGTCGTTGTAGCCACCgg     <  2:2602667/100‑1 (MQ=255)
                                                                                                 ccTAAGGCAAGCCGCCAGACGTTACCAGCATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTc   >  1:3736550/1‑100 (MQ=255)
                                                                                                  cTAAGGTAAGACGCCAGACGTTACCAGTATTGGTTTGCTTTGGATGTATCGAGCATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCATCGGTCt  <  2:2187650/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GCTTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCT  >  NC_000913/3918198‑3918395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: