BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,027,058	C→A	63.9%	R178S (CGT→AGT)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,027,058	0	C	A	63.9%	121.1 / 173.4	153	R178S (CGT→AGT)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand): ref base C (32/23); new base A (44/53); total (77/76)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.16e-01

Only 100 of 154 total aligned reads displayed.

TGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCC  >  NC_000913/4026963‑4027157

tGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTAc                                                                                                 >  2:1774546/1‑100 (MQ=255)

tGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTAc                                                                                                 >  2:2035989/1‑100 (MQ=255)

 gTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                                >  2:605449/1‑100 (MQ=255)

 gTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                                <  1:2139345/100‑1 (MQ=255)

    tGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGc                                                                                             >  1:3732042/1‑100 (MQ=255)

        ctctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCg                                                                                         >  1:1553071/1‑100 (MQ=255)

        ctctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCg                                                                                         >  1:2946148/1‑100 (MQ=255)

         tctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCgt                                                                                        >  2:1307467/1‑100 (MQ=255)

          ctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCgtg                                                                                       >  1:260397/1‑100 (MQ=255)

          ctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCgtg                                                                                       <  1:2568774/100‑1 (MQ=255)

           tGCGAGCTTGAGGCGCTTTCGTTGAAGCATCGTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCgtgt                                                                                      <  2:2661887/100‑1 (MQ=255)

             cGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTa                                                                                    >  2:3550734/1‑100 (MQ=255)

                gCTTGAGTCGCTTGCTTTGAAGCATCCTGGGCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAAcg                                                                                 <  2:733450/100‑1 (MQ=255)

                gCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAAcg                                                                                 <  1:2236439/100‑1 (MQ=255)

                  ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAAcggc                                                                               >  2:1483243/1‑100 (MQ=255)

                  ttGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAACCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAAcggc                                                                               <  2:2103216/100‑1 (MQ=255)

                    gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAAcggcgg                                                                             <  1:159818/100‑1 (MQ=255)

                    gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAAcggcgg                                                                             <  1:2835600/100‑1 (MQ=255)

                    gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAAcggcgg                                                                             <  1:428311/100‑1 (MQ=255)

                    gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAAcggcgg                                                                             >  2:3053865/1‑100 (MQ=255)

                      ggCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAAcggcgg                                                                             >  2:3485188/1‑98 (MQ=255)

                      ggCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTa                                                                           >  1:607630/1‑100 (MQ=255)

                        cgcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTa                                                                           >  2:2848237/1‑98 (MQ=255)

                        cgcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTAtt                                                                         >  2:1507641/1‑100 (MQ=255)

                         gggtgaggtGAAGCATCCTGGTCTGCGAGTGGTGCCGGTGGGTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGGACCGTGTTAAcggcgg                                                                             <  2:3733519/87‑1 (MQ=255)

                         gcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTg                                                                        >  1:3237651/1‑100 (MQ=255)

                         gcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTg                                                                        >  2:2061571/1‑100 (MQ=255)

                         gcTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTg                                                                        <  1:1248098/100‑1 (MQ=255)

                          cTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGc                                                                       >  1:2804053/1‑100 (MQ=255)

                           tttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCa                                                                      >  1:1737042/1‑100 (MQ=255)

                            ttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCa                                                                      <  2:222510/99‑1 (MQ=255)

                            ttCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAg                                                                     <  1:1800653/100‑1 (MQ=255)

                             tCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAgg                                                                    >  1:806056/1‑100 (MQ=255)

                             tCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAgg                                                                    <  2:3056593/100‑1 (MQ=255)

                             tCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAgg                                                                    <  2:1889308/100‑1 (MQ=255)

                               gTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGAt                                                                  >  1:1587487/1‑100 (MQ=255)

                                   aaGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACg                                                              <  1:3550760/100‑1 (MQ=255)

                                     gaaTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGt                                                            >  1:3661958/3‑100 (MQ=255)

                                     gCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGt                                                            <  1:3257696/100‑1 (MQ=255)

                                      cATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTa                                                           <  2:1190471/100‑1 (MQ=255)

                                      cATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTa                                                           <  1:2215228/100‑1 (MQ=255)

                                       aTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTAc                                                          <  1:3770851/100‑1 (MQ=255)

                                       aTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTAc                                                          >  1:750216/1‑100 (MQ=255)

                                        ttttGGTCTGTAAGTGGTGCTGGTGGTTGAACAATTGGAAGCGGGTTGGCGTGGGAGTACTGGTATCGTGTTAATGGCGGTATTGCAGGATCACgg                                                             <  2:1091547/93‑1 (MQ=255)

                                         ccTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGc                                                        >  1:3354497/1‑100 (MQ=255)

                                         ccTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGc                                                        >  1:3354573/1‑100 (MQ=255)

                                          cTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCt                                                       >  2:3722282/1‑100 (MQ=255)

                                             gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                                    <  1:1810805/100‑1 (MQ=255)

                                             gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                                    <  2:1605222/100‑1 (MQ=255)

                                             gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                                    <  2:1277686/100‑1 (MQ=255)

                                             gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGGTAACGGCGGGATTGCAGGATCACGGTACGCTGGc                                                    >  2:2598238/1‑100 (MQ=255)

                                              tCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                                    >  1:2491095/1‑99 (MQ=255)

                                               cTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCGGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCt                                                       >  2:1202434/1‑95 (MQ=255)

                                                 gCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCagag                                                >  2:449460/1‑100 (MQ=255)

                                                  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGc                                               >  1:3215864/1‑100 (MQ=255)

                                                  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGc                                               <  1:2165962/100‑1 (MQ=255)

                                                  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGc                                               <  2:3354550/100‑1 (MQ=255)

                                                  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGc                                               <  2:3354234/100‑1 (MQ=255)

                                                  cAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGc                                               <  1:2024501/100‑1 (MQ=255)

                                                   aaGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCagag                                                <  1:3542052/98‑1 (MQ=255)

                                                    aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCAt                                             >  1:3810358/1‑100 (MQ=255)

                                                     gtggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATg                                            >  2:3859629/1‑100 (MQ=255)

                                                     gtggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATg                                            <  2:1688528/100‑1 (MQ=255)

                                                       ggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCTCGGTACGCTGGCAGAGCATg                                            >  1:177830/1‑98 (MQ=255)

                                                       ggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGat                                          >  2:1630209/1‑100 (MQ=255)

                                                       ggtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGat                                          <  1:467431/100‑1 (MQ=255)

                                                        gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGata                                         <  2:2580353/100‑1 (MQ=255)

                                                          gCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATc                                       >  1:619053/1‑100 (MQ=255)

                                                              gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCtata                                   <  2:81069/100‑1 (MQ=255)

                                                                  ttGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTGAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGc                               <  2:1752958/100‑1 (MQ=255)

                                                                  ttGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGc                               >  1:1086888/1‑100 (MQ=255)

                                                                       caacCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGAc                          >  2:278872/1‑100 (MQ=255)

                                                                        aacCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACg                         <  2:3192571/100‑1 (MQ=255)

                                                                           cGGAAGCGGGCTGGCGTGGGGGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGttt                      >  2:2402585/1‑100 (MQ=255)

                                                                           cGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGttt                      <  2:3591003/100‑1 (MQ=255)

                                                                            ggAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGtttt                     >  1:1015037/1‑100 (MQ=255)

                                                                            ggAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGtttt                     >  2:271917/1‑100 (MQ=255)

                                                                            ggAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGtttt                     <  1:1307476/100‑1 (MQ=255)

                                                                             gAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTg                    <  1:1562708/100‑1 (MQ=255)

                                                                              aaGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTga                   >  2:860723/1‑100 (MQ=255)

                                                                              aaGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTga                   <  2:2619974/100‑1 (MQ=255)

                                                                               aGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTga                   <  2:1734141/99‑1 (MQ=255)

                                                                                gCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTgaga                 <  2:3745876/100‑1 (MQ=255)

                                                                                 cGGGCTGGCGTGGGAGTACTGGCACCGTGTTATCGGCGTTATTGCAGGATTACGGTACGCTGGCAGAGCAGGATATCTATATTGCCGGACGTTTTgaga                 <  2:3316404/99‑1 (MQ=255)

                                                                                 cGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGAt                <  2:2167573/100‑1 (MQ=255)

                                                                                 cGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGAt                <  2:2786441/100‑1 (MQ=255)

                                                                                 cGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGAt                <  2:556072/100‑1 (MQ=255)

                                                                                  gggCTGGCGTGTGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATg               <  2:1960317/100‑1 (MQ=255)

                                                                                  gggCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATg               >  2:619931/1‑100 (MQ=255)

                                                                                  gggCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGAt                >  2:274294/1‑99 (MQ=255)

                                                                                  gggCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATg               >  1:62827/1‑100 (MQ=255)

                                                                                    gCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGc             <  1:2360825/100‑1 (MQ=255)

                                                                                    gCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGGAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGc             <  2:2491078/100‑1 (MQ=255)

                                                                                       ggCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGaa          >  2:3264165/1‑100 (MQ=255)

                                                                                         cGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGaaaa        >  2:517140/1‑100 (MQ=255)

                                                                                         cGTAGGAGTACTGGCACAGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGGTTTGAGGTGGCGgaaa        >  2:3176622/1‑100 (MQ=255)

                                                                                          gTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAt       <  1:2503373/100‑1 (MQ=255)

                                                                                          gTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAt       <  1:2322551/100‑1 (MQ=255)

                                                                                          gTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAt       <  1:278981/100‑1 (MQ=255)

                                                                                              gCGTACTGGCACCGTGTTAACGTCGGTATTGCAGGATCACGGTACGCTGGCAGCGCGTGATATCTATATTGCCGGACGTTTGCAGATCGCGAACATTGc    >  2:3333109/1‑99 (MQ=255)

TGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAATTGCCC  >  NC_000913/4026963‑4027157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`