Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 478,750 C→T 19.1% intergenic (‑133/+31) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913478,7500CT19.1% 419.5 / 84.8 190intergenic (‑133/+31)ylaB/ylaCputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (86/66);  new base T (21/15);  total (108/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 197 total aligned reads displayed.

ATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGAT  >  NC_000913/478652‑478849
                                                                                                  |                                                                                                   
ntatCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCa                                                                                                    >  2:1911702/2‑100 (MQ=255)
   tCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCcgag                                                                                                 >  1:91131/1‑100 (MQ=255)
   tCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCagag                                                                                                 >  1:3676749/1‑100 (MQ=255)
      gATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCtt                                                                                              >  2:1818828/1‑100 (MQ=255)
       aTTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCttt                                                                                             >  1:3070014/1‑100 (MQ=255)
        ttGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTa                                                                                            <  1:287965/100‑1 (MQ=255)
         tgtgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGTCGCAGAGCTTTa                                                                                            >  1:2294313/1‑99 (MQ=255)
          gtgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACg                                                                                          <  2:3191814/100‑1 (MQ=255)
           tgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                                         >  1:192145/1‑100 (MQ=255)
           tgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                                         >  2:1265674/1‑100 (MQ=255)
           tgGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGc                                                                                         >  2:3719254/1‑100 (MQ=255)
            ggAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                                        >  2:1308354/1‑100 (MQ=255)
            ggAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                                        <  2:1004598/100‑1 (MQ=255)
            ggAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGcc                                                                                        >  1:2750786/1‑100 (MQ=255)
             gAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCt                                                                                       >  2:652680/1‑100 (MQ=255)
              aaaaaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTg                                                                                      <  2:3010376/100‑1 (MQ=255)
               aaaaGGCTGGCTGGAGATAAGGAGGTAGACCGGGTATTTCATGAAGTGATGGGTGGAAGCGATCATTGTCATTTGACCATGCGTAAAGCTCTGCGCCtag                                                                                     <  2:2364517/100‑3 (MQ=255)
                 aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                   <  1:1438585/100‑2 (MQ=38)
                 aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                   <  2:2990367/100‑2 (MQ=38)
                 aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                   <  1:2509389/100‑2 (MQ=38)
                 aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                   <  2:1393093/100‑2 (MQ=38)
                 aaGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGTAAAGCTCTGCGCCTGGtc                                                                                   <  2:3222065/100‑2 (MQ=38)
                     ctggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                                               >  1:3229703/1‑100 (MQ=255)
                     ctggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                                               <  1:593/100‑1 (MQ=255)
                     ctggctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTa                                                                               <  1:2243428/100‑1 (MQ=255)
                        gctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAcc                                                                            <  1:2960318/100‑1 (MQ=255)
                        gctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAcc                                                                            >  1:237419/1‑100 (MQ=255)
                         ctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                           >  1:3487344/1‑100 (MQ=255)
                         ctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                           >  2:1090720/1‑100 (MQ=255)
                         ctggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAAccc                                                                           >  1:1462355/1‑100 (MQ=255)
                          tggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCt                                                                          <  1:3719282/100‑1 (MQ=255)
                           ggAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTa                                                                         >  2:2645067/1‑100 (MQ=255)
                              gaTAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACt                                                                      <  2:2243147/100‑1 (MQ=255)
                                 aaGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTaa                                                                   >  2:236042/1‑100 (MQ=255)
                                  aggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACTAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAg                                                                  >  2:1432985/1‑100 (MQ=255)
                                  aggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAg                                                                  >  1:2893120/1‑100 (MQ=255)
                                  aggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCGTTACGCCTGGTATGTAACGCTAACTTAAg                                                                  >  2:2587651/1‑100 (MQ=255)
                                   ggagtTAAAGCTGGTATTTCATGACTTGAGCGGTGGAATCGATCATTGTCATTTGACCAGGCGAAGAGCTTTACGCCTAGTATGTAACCCTAACTTAAGa                                                                 <  2:3853120/100‑1 (MQ=255)
                                   ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                                                 >  2:2623634/1‑100 (MQ=255)
                                   ggaggTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGa                                                                 <  2:3065278/100‑1 (MQ=255)
                                      ggTAGAGCTGGTATTTCATGAAGTGATTGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGt                                                              >  2:790185/1‑100 (MQ=255)
                                       gTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                             <  2:921783/100‑1 (MQ=255)
                                       gTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGtt                                                             <  1:1084653/100‑1 (MQ=255)
                                        tAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTg                                                            <  2:2689520/100‑1 (MQ=255)
                                         agagCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGa                                                           <  1:2543036/100‑1 (MQ=255)
                                          gagCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                                          >  1:3116835/1‑100 (MQ=255)
                                          gagCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAt                                                          >  1:3361907/1‑100 (MQ=255)
                                           agCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGAtt                                                         >  2:2209534/1‑100 (MQ=255)
                                            gCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTc                                                        >  1:1141610/1‑100 (MQ=255)
                                              tGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                                                      >  2:863378/1‑100 (MQ=255)
                                              tGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCgg                                                      >  1:2045388/1‑100 (MQ=255)
                                               ggTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                                     <  2:225302/100‑1 (MQ=255)
                                               ggTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgc                                                     >  2:1560428/1‑100 (MQ=255)
                                                gTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                                                    <  2:3723955/100‑1 (MQ=255)
                                                gTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGgcg                                                    <  1:1595317/100‑1 (MQ=255)
                                                  aTTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGcgcg                                                  <  1:50287/100‑1 (MQ=255)
                                                  aTTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGcgcg                                                  >  2:1067441/1‑100 (MQ=255)
                                                    ttCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                                                <  1:1802362/100‑1 (MQ=255)
                                                    ttCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgag                                                <  2:621232/100‑1 (MQ=255)
                                                     tCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagc                                               <  2:854456/100‑1 (MQ=255)
                                                      cATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                                              >  2:2973899/1‑100 (MQ=255)
                                                      cATGAAGTGATGGCTGGAAACGATCATTGTCATTTGACCAGGAGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcg                                              >  1:2663727/1‑100 (MQ=255)
                                                       aTGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCgcgagcga                                             >  2:3796010/1‑100 (MQ=255)
                                                          aaGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAggg                                          <  2:3283251/100‑1 (MQ=255)
                                                            gTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTa                                        <  1:3796038/100‑1 (MQ=255)
                                                              gATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGTGCGAGCGAGGGTaaa                                      >  1:3895114/1‑100 (MQ=255)
                                                                tGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTaaaaa                                    >  1:1216805/1‑100 (MQ=255)
                                                                 gggTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAat                                   >  1:1248209/1‑100 (MQ=255)
                                                                  ggTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAata                                  <  2:2798529/100‑1 (MQ=255)
                                                                   gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat                                 >  2:145006/1‑100 (MQ=255)
                                                                   gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat                                 >  2:1949323/1‑100 (MQ=255)
                                                                   gTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAatat                                 >  2:3893143/1‑100 (MQ=255)
                                                                    tGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATc                                <  2:3361884/100‑1 (MQ=255)
                                                                     ggAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCg                               <  1:3325283/100‑1 (MQ=255)
                                                                      gAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGt                              <  2:2506542/100‑1 (MQ=255)
                                                                      gAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGt                              >  2:393961/1‑100 (MQ=255)
                                                                               aTTGTCATTTGACGAGGCGCAGAGCTTTACGCGTGGTATGTAAACTTAACTTAAGATGTTTATTCGGCGCGAGCGAGGGTAAAAATATCGTa                             <  2:1616211/92‑1 (MQ=255)
                                                                               aTTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACa                     >  1:2501817/1‑100 (MQ=255)
                                                                                ttGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTaagatcggaa                    >  1:3192884/1‑92 (MQ=255)
                                                                                ttGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAg                    <  1:410188/100‑1 (MQ=255)
                                                                                 tGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGt                   <  2:1468140/100‑1 (MQ=255)
                                                                                   gttacataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTc                 >  2:1485348/8‑100 (MQ=25)
                                                                                    cATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCa                <  2:55179/100‑1 (MQ=255)
                                                                                    cATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCa                >  1:558703/1‑100 (MQ=255)
                                                                                    cATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCa                <  1:2833901/100‑1 (MQ=255)
                                                                                        ataCCAGGCGTAAAGCTCTGCGCCTGGTATGTATCCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCtt            >  2:2607960/3‑100 (MQ=37)
                                                                                        ataCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCtt            >  2:2154340/3‑100 (MQ=38)
                                                                                         taCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt           >  1:2924699/2‑100 (MQ=38)
                                                                                         gACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCttt           >  2:433392/1‑100 (MQ=255)
                                                                                          aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGTCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa          >  1:1009739/1‑100 (MQ=38)
                                                                                          aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTa          >  1:3036392/1‑100 (MQ=38)
                                                                                          aCCAGGCGTAAAGCTCTGCGCCTGGTATGTAACACTACCTTAAGATGTTGCTTCGGCGCAAGCGAGGGTACAAATATCGTACAAAGACAGATCAcccaca          >  2:2710506/1‑96 (MQ=19)
                                                                                           ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  2:3475484/1‑100 (MQ=255)
                                                                                           ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  2:240043/1‑100 (MQ=255)
                                                                                           ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  1:2935208/1‑100 (MQ=255)
                                                                                           ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  1:3626592/1‑100 (MQ=255)
                                                                                           ccAGGCGTAAAGCTCTGCGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  2:1133163/1‑100 (MQ=255)
                                                                                           ccAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTAc         >  1:98971/1‑100 (MQ=255)
                                                                                            cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACg        >  1:2611791/1‑100 (MQ=255)
                                                                                            cAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACg        >  1:3776340/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
ATATCTGATTGTGGAAAAAGGCTGGCTGGAGATAAGGAGGTAGAGCTGGTATTTCATGAAGTGATGGGTGGAAACGATCATTGTCATTTGACCAGGCGCAGAGCTTTACGCCTGGTATGTAACCCTAACTTAAGATGTTGATTCGGCGCGAGCGAGGGTAAAAATATCGTAAAAAGACAGTTCACCTTTACGGACGAT  >  NC_000913/478652‑478849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: