Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 87,887 C→T 15.6% intergenic (+39/‑141) ilvH → / → cra acetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091387,8870CT15.6% 386.9 / 52.1 159intergenic (+39/‑141)ilvH/craacetolactate synthase 3, small subunit, valine‑sensitive/transcriptional repressor‑activator for carbon metabolism
Reads supporting (aligned to +/- strand):  ref base C (64/70);  new base T (10/15);  total (74/85)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 161 total aligned reads displayed.

GTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAG  >  NC_000913/87789‑87986
                                                                                                  |                                                                                                   
  ggAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGtt                                                                                                  >  2:278055/1‑100 (MQ=255)
   gAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATTATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg                                                                                                 <  2:2427479/100‑1 (MQ=255)
     ggTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTggg                                                                                               >  1:274998/1‑100 (MQ=255)
      gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGc                                                                                              <  1:371899/100‑1 (MQ=255)
      gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGc                                                                                              <  2:1704090/100‑1 (MQ=255)
      gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                              >  1:477318/1‑100 (MQ=255)
      gTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGc                                                                                              <  2:162003/100‑1 (MQ=255)
       ttGCTCGCTCTGGTGTGTTCGGACTTTCTCTCGGAGATAAAAGAATGCGTTGAGAATTATCTCAATCCGCAAGTTACAGCCCAACATGTCACGTTGGGc                                                                                              <  2:544908/99‑1 (MQ=255)
        tGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                            <  2:2747595/100‑2 (MQ=255)
         gctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgt                                                                                           <  2:649342/100‑3 (MQ=255)
          ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgta                                                                                          <  2:172280/100‑4 (MQ=38)
          ctcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt                                                                                          <  1:152069/100‑1 (MQ=255)
          ctcgctcTGGGGTGGTCGCACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTTAATGCGCAATTTACAGCCCAACGTGACCTTTTGGGCtgta                                                                                          <  2:93651/100‑4 (MQ=255)
           tcgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttt                                                                                         >  2:3930490/1‑100 (MQ=255)
            cgctcTTGTGTGGTCGGACTTTCACGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtg                                                                                            <  2:3651271/96‑2 (MQ=255)
            cgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGCATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttt                                                                                        >  2:486244/1‑100 (MQ=255)
            cgctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTTACGTTGGGCtttttt                                                                                        >  1:3330820/1‑100 (MQ=255)
             gctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTGGGCtgtaaat                                                                                       <  1:2698398/100‑7 (MQ=38)
             gctcTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttttt                                                                                       <  1:2888891/100‑1 (MQ=255)
              ctctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg                                                                                      <  1:1762193/100‑1 (MQ=255)
                ctGGTGTGGTCGTTCTTTCGCGTGGCGTTAAAATAATGCGTTGAGTTTGAGCTCAATGCGCAGTTTACAGTCTAACGTGACATGTTGGGCtgtaaattgc                                                                                    <  2:2641844/100‑10 (MQ=16)
                ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  1:821884/100‑1 (MQ=255)
                ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  2:3332972/100‑1 (MQ=255)
                ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  2:1149671/100‑1 (MQ=255)
                ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  1:1822446/100‑1 (MQ=255)
                ctGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGACCTCAATGCGCAATTTTCAGCCCAACATGTCACGTTGGGCTTTTTTTGCg                                                                                    <  2:2595563/100‑1 (MQ=255)
                   gtgtGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaaa                                                                                 >  2:2316037/1‑100 (MQ=255)
                       ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                             >  2:141000/1‑100 (MQ=255)
                       ggTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg                                                                             >  1:3808423/1‑100 (MQ=255)
                        gTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGt                                                                            >  1:2877044/1‑100 (MQ=255)
                         tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTgagatcggaaga                                                                           >  1:3049200/1‑92 (MQ=255)
                         tCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg                                                                           <  2:2128386/100‑1 (MQ=255)
                          cGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg                                                                          <  2:415497/100‑1 (MQ=255)
                            gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGa                                                                        >  2:1532915/1‑100 (MQ=255)
                                ttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCt                                                                    >  1:663098/1‑100 (MQ=255)
                                 ctgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTg                                                                   <  2:872917/98‑1 (MQ=255)
                                  cgcgctGCGATAAAATAAGGCTTTGAGAAGGATCTCAATGCGCGACTTACGGCCCAAACTATCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTg                                                                   <  2:238283/99‑1 (MQ=255)
                                  cgcgcgGCTATAAAATAATGCGTTGAGAATGCTCTCAATGCGCAATTTACATCCCAACATGTCACGTTGGGCTTTTTTTTCGCAATCAGTAGGAACCt                                                                    >  2:782898/1‑98 (MQ=255)
                                   gcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgag                                                                 >  1:872921/1‑98 (MQ=255)
                                    cgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGaa                                                                >  1:2225169/1‑100 (MQ=255)
                                     gcggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAAt                                                               >  2:261623/1‑100 (MQ=255)
                                         cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaaa                                                           >  1:1946290/1‑100 (MQ=255)
                                         cgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATATACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaaaa                                                           >  1:1182923/1‑100 (MQ=255)
                                           aTAAAATAATGCGTTTAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                         <  2:2281383/100‑1 (MQ=255)
                                           aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                         >  2:2184383/1‑100 (MQ=255)
                                           aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc                                                         <  2:3738706/100‑1 (MQ=255)
                                            tAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCa                                                        >  1:958018/1‑100 (MQ=255)
                                                  aatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc                                                  <  1:3764015/100‑1 (MQ=255)
                                                    tGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGTGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc                                                <  2:2112191/100‑1 (MQ=255)
                                                    tGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc                                                <  1:1879240/100‑1 (MQ=255)
                                                      cggtGAGAATTATCTCAATGCGCTATTTACAGCCCAACATGTCACGGTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAATAGCAGTTGc                                                   <  2:3134693/92‑1 (MQ=255)
                                                       gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                             <  1:3180289/100‑1 (MQ=255)
                                                       gTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGt                                             >  1:580756/1‑100 (MQ=255)
                                                        ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGtt                                            <  1:2324865/100‑1 (MQ=255)
                                                          gagatcattctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTaa                                          >  2:2574567/9‑100 (MQ=255)
                                                           agaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAt                                         >  2:3723168/1‑100 (MQ=255)
                                                            gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGGTTTTTTTGCGAAATCAGTGGGAACCTGGAATTAAAGCAGTTGCAGCAGTTAAtt                                        >  2:3445108/1‑100 (MQ=255)
                                                               tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTc                                     <  1:3839835/100‑1 (MQ=255)
                                                               tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTc                                     <  1:261623/100‑1 (MQ=255)
                                                                gATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTca                                    >  1:3725827/1‑99 (MQ=255)
                                                                 aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                   <  2:3090800/100‑1 (MQ=255)
                                                                 aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTg                                   >  1:3643098/1‑100 (MQ=255)
                                                                 aTCTCAATGCGCAATTTACAGCACAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATACAAGAAGTTGCCGCAGTTAATTTTCt                                    >  2:856767/1‑99 (MQ=255)
                                                                  tctcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgc                                  >  1:524099/1‑100 (MQ=255)
                                                                    tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGTCTGTTTTTGGTAAATCAGTGGGAAACTGGAATGAAAGCAGTTGCCGCAGTTAATTTTCt                                    >  2:2915143/1‑96 (MQ=255)
                                                                    tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgcgc                                >  1:1282547/1‑100 (MQ=255)
                                                                    tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGAGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTgcgc                                >  1:450621/1‑100 (MQ=255)
                                                                        tGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAg                            <  2:3828297/100‑1 (MQ=255)
                                                                         gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                           <  1:3584534/100‑1 (MQ=255)
                                                                         gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                           >  2:3462259/1‑100 (MQ=255)
                                                                         gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGa                           >  1:2322974/1‑100 (MQ=255)
                                                                           gcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATg                         >  2:1204658/1‑100 (MQ=255)
                                                                            gcaaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                        >  2:1767668/9‑100 (MQ=37)
                                                                            cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                        <  2:1909217/100‑1 (MQ=255)
                                                                            cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGt                        <  2:682652/100‑1 (MQ=255)
                                                                             caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  1:3655682/8‑100 (MQ=38)
                                                                             caaaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  2:3321262/8‑100 (MQ=37)
                                                                             aaTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       >  1:1562136/1‑100 (MQ=255)
                                                                              aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGtt                       <  2:1187071/99‑1 (MQ=255)
                                                                              aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                      <  1:2215286/100‑1 (MQ=255)
                                                                              aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTa                      <  2:2366349/100‑1 (MQ=255)
                                                                               tttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTaa                     <  1:497233/100‑1 (MQ=255)
                                                                               aaaaaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTaa                     >  2:1694487/6‑100 (MQ=38)
                                                                                ttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAAt                    <  1:1329430/100‑1 (MQ=255)
                                                                                 tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTATTg                   >  1:2721531/1‑100 (MQ=255)
                                                                                 atctgCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGGGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATg                   <  2:2764229/96‑1 (MQ=255)
                                                                                  aaaGCCCACCGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAAGAAAAGCAGCTTCCGCAGTTAATTTTCTGCGCCTAGATGTTACTg                   >  2:3474525/3‑99 (MQ=255)
                                                                                  aaaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGa                  >  1:3058464/3‑100 (MQ=255)
                                                                                   cAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa                 >  1:1264157/1‑100 (MQ=255)
                                                                                   aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa                 >  1:2417087/2‑100 (MQ=255)
                                                                                   aaGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGaa                 >  1:2423923/2‑100 (MQ=255)
                                                                                    aGCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                >  1:3545416/1‑100 (MQ=255)
                                                                                    aGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAt                <  1:2308991/100‑1 (MQ=255)
                                                                                     gCCCAACGTGACATGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGTAtt               >  1:3547093/1‑100 (MQ=255)
                                                                                     gCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAAtt               >  1:2140100/1‑100 (MQ=255)
                                                                                         aaCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAc           <  2:53101/100‑1 (MQ=255)
                                                                                           cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAccc         <  1:919647/100‑1 (MQ=255)
                                                                                           cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAAcc          <  2:3771674/99‑1 (MQ=255)
                                                                                            aTGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCa        >  1:1096265/1‑100 (MQ=255)
                                                                                                  cGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAg  <  1:3427646/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTGGAGGTTGCTCGCTCTGGTGTGGTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAG  >  NC_000913/87789‑87986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: