Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,211,610 A→G 15.0% intergenic (+33/+70) icdC → / ← iraM pseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,211,6100AG15.0% 429.7 / 61.5 173intergenic (+33/+70)icdC/iraMpseudogene, isocitrate dehydrogenase C‑terminal gene fragment; idcC' is a 54 codon 3' gene fragment created during e14 prophage insertion/RpoS stabilzer during Mg starvation, anti‑RssB factor
Reads supporting (aligned to +/- strand):  ref base A (85/62);  new base G (12/14);  total (97/76)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 188 total aligned reads displayed.

TCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTA  >  NC_000913/1211511‑1211708
                                                                                                   |                                                                                                  
tCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGta                                                                                                    >  1:1226799/1‑100 (MQ=255)
 tccgatctaGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtat                                                                                                   <  1:1681952/92‑1 (MQ=255)
 ntGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtat                                                                                                   >  1:2629003/2‑100 (MQ=255)
  tGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATTTCTCCATGTGTTAAATATTGAAACGGGCGtata                                                                                                  <  2:3618339/100‑1 (MQ=255)
  tGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGtata                                                                                                  >  1:1380747/1‑100 (MQ=255)
    aTGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAac                                                                                                <  2:1594584/100‑1 (MQ=255)
      ggAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAacac                                                                                              >  1:472327/1‑100 (MQ=255)
        aaGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGc                                                                                            >  2:2177546/1‑100 (MQ=255)
        aaGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGc                                                                                            >  1:1666030/1‑100 (MQ=255)
         aGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGcc                                                                                           <  2:186450/100‑1 (MQ=255)
            cgcTAAGCTGCTGAGATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGGAATCTCTCCATGTGTTAAATATTGAAACGGTCGTGTTATACTCCCgt                                                                                        <  2:1149000/100‑1 (MQ=21)
             gcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtt                                                                                       <  2:3116861/100‑1 (MQ=255)
              cTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGAGCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgttt                                                                                      <  1:3761956/100‑2 (MQ=25)
                aaGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttca                                                                                    <  2:3187324/100‑4 (MQ=25)
                aaGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttc                                                                                     <  2:1001916/99‑3 (MQ=25)
                aaGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCgttgtt                                                                                    >  2:3687799/1‑100 (MQ=255)
                 aGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaa                                                                                   <  2:1703730/100‑5 (MQ=25)
                 aGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaa                                                                                   <  1:1281256/100‑5 (MQ=25)
                   ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaata                                                                                 <  1:1582624/100‑7 (MQ=21)
                   ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTGTTATACGCCCgtttcaata                                                                                 <  1:2708032/100‑7 (MQ=25)
                   ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTttta                                                                                 >  1:1553693/1‑100 (MQ=255)
                   ctgctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTttta                                                                                 <  1:2486741/100‑1 (MQ=255)
                     gctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttatt                                                                               <  2:1256993/100‑1 (MQ=255)
                      ctgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttattt                                                                              <  1:3854944/100‑1 (MQ=255)
                       tgAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTtttattta                                                                             >  2:3583891/1‑100 (MQ=255)
                           aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTAtgtg                                                                         <  1:595977/100‑1 (MQ=255)
                           aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGGGGAGGGTTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTAtgtg                                                                         <  1:1111819/100‑1 (MQ=37)
                           aTGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGAGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTAtgtg                                                                         <  2:84746/100‑1 (MQ=255)
                            tGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTgg                                                                        <  2:1201602/100‑1 (MQ=255)
                             gTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGa                                                                       >  2:1697772/1‑100 (MQ=255)
                             gTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGa                                                                       >  2:3791915/1‑100 (MQ=255)
                             gTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGa                                                                       >  1:1400618/1‑100 (MQ=255)
                              ttCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                      >  1:3029729/1‑100 (MQ=255)
                               tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGata                                                                     <  2:891354/100‑1 (MQ=255)
                               tCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGat                                                                      >  1:1944538/1‑99 (MQ=255)
                                cAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGatat                                                                    <  1:2642551/100‑1 (MQ=255)
                                cAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGatat                                                                    >  1:649854/1‑100 (MQ=255)
                                   agTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                 <  2:3034882/100‑1 (MQ=255)
                                   agTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                 <  1:3731285/100‑1 (MQ=255)
                                   agTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                 <  1:3671149/100‑1 (MQ=255)
                                   agTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGAtattat                                                                 >  2:2831574/1‑100 (MQ=255)
                                    gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATattatt                                                                <  1:3491400/100‑1 (MQ=255)
                                    gTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCAATGTGTTACATATTGAAACGGGCGAATAACACGCCCGTTATTTTATTTATGTGGATcttatt                                                                >  2:3380998/1‑100 (MQ=38)
                                     tttGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATAttatta                                                               <  1:3854211/100‑1 (MQ=255)
                                      ttGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTaa                                                              <  1:1167011/100‑1 (MQ=255)
                                        ggTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATa                                                            >  2:2030324/1‑100 (MQ=255)
                                        ggTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATa                                                            >  1:720313/1‑100 (MQ=255)
                                         gTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAg                                                           <  1:82921/100‑1 (MQ=255)
                                          tGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAACCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGc                                                          <  1:196428/100‑1 (MQ=255)
                                           gAAGCGATCATCTAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGc                                                          <  2:1248192/99‑1 (MQ=255)
                                                gATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCg                                                    >  1:1576408/1‑100 (MQ=255)
                                                 atcatcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGa                                                   >  2:2019232/1‑100 (MQ=255)
                                                   catcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                 <  2:3096963/100‑1 (MQ=255)
                                                    atcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCa                                                <  2:3471649/100‑1 (MQ=255)
                                                     tcGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCat                                               >  1:2965649/1‑100 (MQ=255)
                                                      cGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGc                                                 <  2:513988/97‑1 (MQ=255)
                                                       gAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCatat                                             >  2:2667010/1‑100 (MQ=255)
                                                          aaCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATCGCATATCGAGCATATTta                                          <  2:3294460/100‑1 (MQ=255)
                                                           aCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtat                                         <  2:1345297/100‑1 (MQ=255)
                                                           aCATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtat                                         <  1:3630129/100‑1 (MQ=255)
                                                            cATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtata                                        <  1:2015972/100‑1 (MQ=255)
                                                            cATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTtata                                        <  1:333568/100‑1 (MQ=255)
                                                               gTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGa                                     <  2:37279/100‑1 (MQ=255)
                                                               gTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGa                                     >  1:1917089/1‑100 (MQ=255)
                                                                   tctctcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGcc                                 <  2:939775/100‑1 (MQ=255)
                                                                   tctctcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGcc                                 <  1:3187296/100‑1 (MQ=255)
                                                                    ctctcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGcc                                 >  2:2754675/1‑99 (MQ=255)
                                                                       tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAtt                             >  1:2847906/1‑100 (MQ=255)
                                                                       tcCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCAtt                             >  1:3943253/1‑100 (MQ=255)
                                                                        ccATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGGTATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTa                            >  2:384764/1‑100 (MQ=255)
                                                                        ccATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAATCCCAtt                             >  1:2810251/1‑99 (MQ=255)
                                                                        ccATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTa                            >  2:737620/1‑100 (MQ=255)
                                                                        ccATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTa                            >  1:703962/1‑100 (MQ=255)
                                                                         cATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCTAGCATATTTATATGAAGCACATTAc                           >  2:436866/1‑100 (MQ=255)
                                                                         cATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTAc                           >  1:2305764/1‑100 (MQ=255)
                                                                         cATGGGTTAAATATTGAAACGGGCGTATCACACGCCCGTTGGTTTATCTATGTGGATATTATTAATACCATATCGAGCATATTTATATGAAGACCact                             <  2:2420829/98‑3 (MQ=255)
                                                                            gtgtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTg                        <  1:3276308/100‑1 (MQ=255)
                                                                              gtTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAg                      <  1:56670/100‑1 (MQ=255)
                                                                                tAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGcc                    <  2:2612347/100‑1 (MQ=255)
                                                                                tAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGcc                    <  1:1793369/100‑1 (MQ=255)
                                                                                 aaaTATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGccc                   >  1:1438434/1‑100 (MQ=255)
                                                                                  aataaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCa                  >  2:3895734/9‑100 (MQ=37)
                                                                                    tatTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCACATTACTTGAGCCCata                >  2:485587/1‑100 (MQ=255)
                                                                                     aaaacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCatat               >  1:3870063/6‑100 (MQ=38)
                                                                                      ttGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATg              >  1:2855587/1‑100 (MQ=255)
                                                                                       aacaaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg             >  1:1097226/4‑100 (MQ=38)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg            <  2:948612/100‑1 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg            <  1:1846719/100‑1 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg            <  2:534164/100‑1 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATggg            <  1:3260112/100‑1 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg             >  2:578061/1‑99 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg             >  2:650040/1‑99 (MQ=255)
                                                                                        gAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATgg             >  1:92633/1‑99 (MQ=255)
                                                                                         aaaCGGGCGTATAACACGCCAGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAATCTCATTACTTAAGCCCATATg              <  2:142736/97‑1 (MQ=255)
                                                                                          aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa          >  1:2312565/1‑100 (MQ=255)
                                                                                          aaCGGGCGTGTTATACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCa          >  1:505870/1‑100 (MQ=255)
                                                                                           aCGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAACATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCat         >  1:1711062/1‑100 (MQ=255)
                                                                                                  tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta  <  1:2241510/100‑1 (MQ=255)
                                                                                                  tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta  >  2:1232794/1‑100 (MQ=255)
                                                                                                  tataACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTta  >  2:2792289/1‑100 (MQ=255)
                                                                                                   |                                                                                                  
TCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAATCTCTCCATGTGTTAAATATTGAAACGGGCGTATAACACGCCCGTTGTTTTATTTATGTGGATATTATTAATAGCATATCGAGCATATTTATATGAAGCCCATTACTTGAGCCCATATGGGCATATTTTTA  >  NC_000913/1211511‑1211708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: