Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,620,227 C→G 16.0% intergenic (+20/+11) marB → / ← eamA periplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,620,2270CG16.0% 401.4 / 64.9 149intergenic (+20/+11)marB/eamAperiplasmic mar operon regulator/cysteine and O‑acetyl‑L‑serine efflux system
Reads supporting (aligned to +/- strand):  ref base C (58/64);  new base G (8/15);  total (68/81)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

Only 100 of 154 total aligned reads displayed.

TGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATT  >  NC_000913/1620129‑1620326
                                                                                                  |                                                                                                   
tGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACAtct                                                                                                    >  1:2252822/1‑100 (MQ=255)
  aTTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCg                                                                                                  >  2:25341/1‑100 (MQ=255)
    ttAAATCACATGGGGAGTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCggg                                                                                                <  2:3272973/100‑1 (MQ=255)
      aaaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGc                                                                                              <  2:2328105/100‑1 (MQ=255)
       aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcc                                                                                             <  1:668822/100‑2 (MQ=255)
       aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGCCGGGGcc                                                                                             <  1:2072021/100‑2 (MQ=255)
       aaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTAGCCCCGAGATGTCGGGGcc                                                                                             <  2:209350/100‑2 (MQ=255)
        aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcca                                                                                            <  1:1087243/100‑3 (MQ=255)
        aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGcc                                                                                             <  2:3202647/99‑2 (MQ=255)
        aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCtt                                                                                            >  1:414511/1‑100 (MQ=255)
         tCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                           <  2:2645837/100‑4 (MQ=255)
         tCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                           <  2:1641222/100‑4 (MQ=255)
          cacaTGGTTAATGGAAGTGATAATTCGGATGGGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTGTGTTCTGGCCCCGACATTTCGGGGCttat                                                                                          <  2:624123/100‑1 (MQ=255)
          cacaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccag                                                                                           <  1:568215/99‑4 (MQ=255)
           acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccagaa                                                                                         <  1:1768099/100‑6 (MQ=255)
           acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGAGATGTCGGGGccagaa                                                                                         <  1:2489972/100‑6 (MQ=255)
           acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                         <  1:3174570/100‑1 (MQ=255)
           acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                         <  1:2870505/100‑1 (MQ=255)
            caTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCttatta                                                                                        >  1:100202/1‑100 (MQ=255)
            caTGGGTACTGGAAGTAACAAGTCGGATGCGCTCGGCGTGCAATATTATCATCGACACGCTCTGAAGTTTGCTCTGGCCCCGAGATGTCGgcgcaagaa                                                                                         <  2:1561588/99‑6 (MQ=255)
              tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAAc                                                                                      >  2:3496365/1‑100 (MQ=255)
                ggTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACtt                                                                                    >  1:1420797/1‑100 (MQ=255)
                 gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                   <  2:3249578/100‑1 (MQ=255)
                 gTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                   <  1:1261501/100‑1 (MQ=255)
                  atcTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                  <  1:3068232/98‑1 (MQ=255)
                    cTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCa                                                                                >  1:2874517/1‑100 (MQ=255)
                      ggCAGTGATAAGTCGGATGCGTTCGGCGAGACCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCACAcc                                                                              >  2:1671214/1‑100 (MQ=255)
                      ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAcc                                                                              >  1:258632/1‑100 (MQ=255)
                      ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAcc                                                                              <  1:2613514/100‑1 (MQ=255)
                      ggCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCAcc                                                                              <  1:775488/100‑1 (MQ=255)
                       gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCt                                                                             <  1:2169360/100‑1 (MQ=255)
                         aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCttt                                                                           >  1:453039/1‑100 (MQ=255)
                            gATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAcc                                                                        >  2:2898165/1‑100 (MQ=255)
                            gATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAcc                                                                        <  1:2936689/100‑1 (MQ=255)
                             aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACTTCTCGGGGCTTATTAACTTCCCACCTTTACCg                                                                       <  1:3740397/100‑1 (MQ=255)
                             aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCg                                                                       <  2:3013335/100‑1 (MQ=255)
                               aaGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCt                                                                     >  1:1962785/1‑100 (MQ=255)
                                aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCtt                                                                    >  1:3067749/1‑100 (MQ=255)
                                aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCtt                                                                    >  1:1325726/1‑100 (MQ=255)
                                aGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCtt                                                                    >  2:1969012/1‑100 (MQ=255)
                                 gTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCttt                                                                   >  1:1713144/1‑100 (MQ=255)
                                  tCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTa                                                                  <  2:3818403/100‑1 (MQ=255)
                                   cGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAc                                                                 >  1:1585593/1‑100 (MQ=255)
                                    ggATGCGCTCGGTGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                <  1:775239/100‑1 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTATTTTGTTCTGGCCCCGACATCTCGGGGCTTATTTACTTCCCACCTTTACCGCTTTACg                                                                <  2:238843/100‑1 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTGACTTCCCACCTTTACCGCTTTACg                                                                <  2:1024616/100‑1 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                <  2:1758783/100‑1 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                >  2:2094399/1‑100 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                >  2:3718296/1‑100 (MQ=255)
                                    ggATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACg                                                                <  2:3043308/100‑1 (MQ=255)
                                     gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                               <  1:3730286/100‑1 (MQ=255)
                                         cgcTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                               >  2:1954275/1‑96 (MQ=255)
                                            tCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCa                                                        <  2:2511432/100‑1 (MQ=255)
                                              ggCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAg                                                      <  1:562263/100‑1 (MQ=255)
                                               gCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCATCTTTACCGCTTTACGCCACCGCAAGc                                                     <  1:2404557/100‑1 (MQ=255)
                                                cGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGcc                                                    <  1:3352236/100‑1 (MQ=255)
                                                 gTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCa                                                   >  1:3647773/1‑100 (MQ=255)
                                                 gTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCa                                                   <  2:573510/100‑1 (MQ=255)
                                                    cccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAAt                                                <  1:1528339/100‑1 (MQ=255)
                                                     ccTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                               >  1:3500216/1‑100 (MQ=255)
                                                      cTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATTTCGGGTCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATAc                                              >  2:3383897/1‑100 (MQ=255)
                                                      cTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                               <  1:1740460/99‑1 (MQ=255)
                                                      cTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATAc                                              >  1:3763804/1‑100 (MQ=255)
                                                      cTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATAc                                              >  1:3545967/1‑100 (MQ=255)
                                                          tataATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                          <  1:3235085/100‑1 (MQ=255)
                                                          tataATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTg                                          <  2:716612/100‑1 (MQ=255)
                                                            taATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGat                                        >  1:3774471/1‑100 (MQ=255)
                                                             aaTCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                       >  1:1467835/1‑100 (MQ=255)
                                                               tCAACACGCTATGTAGTTTGTTCTGGCCCCGACCTTTCGGGGCTTATTAACTTCGCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGata                                       <  2:704455/98‑1 (MQ=255)
                                                               tCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGAtata                                     >  2:1128684/1‑100 (MQ=255)
                                                                 aaCACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACa                                   >  2:901051/1‑100 (MQ=255)
                                                                     cGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGccc                               >  2:2759595/1‑100 (MQ=255)
                                                                      gCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCg                              >  1:2408655/1‑100 (MQ=255)
                                                                       cTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCgg                             >  1:248627/1‑100 (MQ=255)
                                                                         aTGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                           <  1:3496390/100‑1 (MQ=255)
                                                                           gTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCAt                         >  1:708927/1‑100 (MQ=255)
                                                                             aGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATaa                       >  2:1797096/1‑100 (MQ=255)
                                                                              gTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                      <  2:2288529/100‑1 (MQ=255)
                                                                              gTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAAt                      <  1:3333230/100‑1 (MQ=255)
                                                                               tttGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTGCGCCACCGCAAGCCAAATACATGGATATACAGCCCGGTCATAATg                     <  2:1103575/100‑1 (MQ=255)
                                                                               tttGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                     <  2:309900/100‑1 (MQ=255)
                                                                                gttaataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATg                     >  2:3352893/9‑99 (MQ=38)
                                                                                 tGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAg                   >  1:71928/1‑100 (MQ=255)
                                                                                 tGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAg                   >  1:2928188/1‑100 (MQ=255)
                                                                                  gTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgc                  <  2:1325717/100‑1 (MQ=255)
                                                                                   ttCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCGAGCCAATTACATTGATATACAGCCCGGTCATAATGAgca                 >  2:2768962/1‑100 (MQ=255)
                                                                                   aataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgca                 >  1:3649100/6‑100 (MQ=255)
                                                                                    ataagCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcac                >  1:2189586/5‑100 (MQ=255)
                                                                                     taagCCCCGAGATGTCGGGGCTTATTAACTTCCCACGTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcacc               >  1:2782239/4‑100 (MQ=255)
                                                                                     cTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcacc               <  2:816433/100‑1 (MQ=255)
                                                                                      tGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcacc               >  2:1178452/1‑99 (MQ=255)
                                                                                       ngCCCCGAGATGTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgc             >  2:2964152/2‑100 (MQ=255)
                                                                                         ccccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcac           >  2:8470/1‑100 (MQ=255)
                                                                                          cccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgctccgcacc          <  1:2078396/100‑1 (MQ=255)
                                                                                          cccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccgcacc          >  1:869590/1‑100 (MQ=255)
                                                                                           ccGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCt         >  2:2410557/1‑100 (MQ=255)
                                                                                              aCATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTaaa      <  1:2220471/100‑1 (MQ=255)
                                                                                              aCATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTaaa      <  2:3948339/100‑1 (MQ=255)
                                                                                               cATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTaaaa     >  1:1245631/1‑100 (MQ=255)
                                                                                               cATCTCGGGGCTTATTAACTTCCCACCTTTACCACTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGATCATAATGAGCACCGCACCTaaaa     >  2:390617/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATT  >  NC_000913/1620129‑1620326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: