Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,764,701 G→A 15.7% intergenic (‑315/+233) sufA ← / ← ydiH Fe‑S cluster assembly protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,764,7010GA15.7% 385.4 / 53.9 158intergenic (‑315/+233)sufA/ydiHFe‑S cluster assembly protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (77/56);  new base A (12/13);  total (89/69)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.03e-01

Only 100 of 163 total aligned reads displayed.

TGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCG  >  NC_000913/1764603‑1764800
                                                                                                   |                                                                                                   
tgaAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGc                                                                                                    <  1:735899/100‑1 (MQ=255)
 gaaaaagaaaaaGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCt                                                                                                   <  1:1927070/100‑1 (MQ=255)
 gaaaaagaaaaaGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAGTGCAGCGTTAAGAAATAAAAAAACCCTTAAGTCTGTGCGACACAGGCt                                                                                                   >  2:1192644/1‑99 (MQ=255)
  aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCtt                                                                                                  >  2:1325751/1‑100 (MQ=255)
  aaaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCt                                                                                                   <  2:364492/99‑1 (MQ=255)
   aaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTa                                                                                                 >  1:1067264/1‑100 (MQ=255)
   aaaagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTa                                                                                                 >  2:1571127/1‑100 (MQ=255)
     aagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAg                                                                                               <  2:1500786/100‑1 (MQ=255)
     aagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAg                                                                                               <  2:1736096/100‑1 (MQ=255)
     aagaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAg                                                                                               <  2:402535/100‑1 (MQ=255)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                              >  1:2710238/1‑100 (MQ=255)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAgg                                                                                              >  1:3450296/1‑100 (MQ=255)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                              <  1:513161/100‑1 (MQ=37)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                              <  2:3681584/100‑1 (MQ=37)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                              <  2:1676621/100‑1 (MQ=37)
      agaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAgg                                                                                              <  1:2461452/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAggg                                                                                             <  2:3279640/100‑1 (MQ=255)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAggg                                                                                             >  2:3226763/1‑100 (MQ=255)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  1:1507293/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  2:700013/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  1:3182908/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  1:3657292/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  1:50648/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGCCTGTGTCGCACAGACTTAAggg                                                                                             <  2:3449390/100‑1 (MQ=37)
       gaaaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTAAGAAATAAGAAAACGCTTAAGTCAGTGCGACACAGGCTTAAgg                                                                                              >  2:1260010/1‑99 (MQ=255)
         aaaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGtt                                                                                           >  1:1945664/1‑100 (MQ=255)
          aaagTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGttt                                                                                          <  1:3834691/100‑1 (MQ=255)
            agTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCt                                                                                        <  1:3319905/100‑1 (MQ=255)
               tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                                     <  1:290782/100‑1 (MQ=255)
               tGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAcc                                                                                     <  2:462514/100‑1 (MQ=255)
                gTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTAccc                                                                                    >  2:3387954/1‑100 (MQ=255)
                  aTTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCa                                                                                  >  1:3047698/1‑100 (MQ=255)
                    tGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATc                                                                                <  1:2872165/100‑1 (MQ=255)
                     gAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATcc                                                                               >  1:331736/1‑100 (MQ=255)
                       aaaTGACTATTTAAGAGATAGGTAAATAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCgg                                                                             >  2:1366626/1‑100 (MQ=255)
                         aTGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGgcg                                                                           >  1:1100947/1‑100 (MQ=255)
                            aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                                        <  1:759192/100‑1 (MQ=255)
                            aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                                        >  1:1582957/1‑100 (MQ=255)
                            aCTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCtt                                                                        <  1:2609265/100‑1 (MQ=255)
                             cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                                       <  2:751924/100‑1 (MQ=255)
                             cTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTa                                                                       <  2:331736/100‑1 (MQ=255)
                              tATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAt                                                                      >  2:2610338/1‑100 (MQ=255)
                              tATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAt                                                                      >  2:1133903/1‑100 (MQ=255)
                               aTTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtc                                                                     <  2:190381/100‑1 (MQ=255)
                                tttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtct                                                                    >  2:2847791/1‑100 (MQ=255)
                                tttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtct                                                                    >  1:7254/1‑100 (MQ=255)
                                 ttAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTAtctc                                                                   >  2:3226673/1‑100 (MQ=255)
                                  tAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTcc                                                                  >  1:1042298/1‑100 (MQ=255)
                                    agagATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCgg                                                                <  1:3853402/100‑1 (MQ=255)
                                     gagaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGc                                                               >  1:1144725/1‑100 (MQ=255)
                                       gaTAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCAc                                                             >  2:1417884/1‑100 (MQ=255)
                                         tAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActc                                                           <  2:2061944/100‑1 (MQ=255)
                                          agatAACAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGGGCGACACAGGCTTAAGGGTTGCGACCCCATCCGGCGCTTATCTCCGGCActc                                                           <  2:847967/96‑1 (MQ=255)
                                          aGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCActct                                                          >  1:2018221/1‑100 (MQ=255)
                                            gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                                        >  1:162194/1‑100 (MQ=255)
                                            gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                                        >  1:2177553/1‑100 (MQ=255)
                                            gTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCa                                                        >  1:3076088/1‑100 (MQ=255)
                                              aacaaaGTGCAGCGTTCAGCAATGAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGt                                                      <  1:2556300/97‑1 (MQ=255)
                                              aaaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAATGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGt                                                      >  2:1291934/1‑100 (MQ=255)
                                               aaaaaGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTg                                                     >  1:2798918/1‑100 (MQ=255)
                                                 aaaGTGCAACGTTCAAAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTgg                                                    <  2:2745297/99‑1 (MQ=255)
                                                        aGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctc                                            <  1:1567387/100‑1 (MQ=255)
                                                         gCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctct                                           >  1:3816364/1‑100 (MQ=255)
                                                         gCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGctct                                           <  2:2710221/100‑1 (MQ=255)
                                                              cAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAg                                      >  2:454049/1‑100 (MQ=255)
                                                                gAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAggg                                    >  1:3149435/1‑100 (MQ=255)
                                                                 aaaTAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAgggg                                   >  1:87690/1‑100 (MQ=255)
                                                                    tAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGa                                >  2:56439/1‑100 (MQ=255)
                                                                      aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGTCTTAGCTCTTGAAGGGGCGATa                              >  1:1819728/1‑100 (MQ=255)
                                                                      aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                              >  2:1364056/1‑100 (MQ=255)
                                                                      aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                              <  1:1018829/100‑1 (MQ=255)
                                                                      aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                              <  2:500342/100‑1 (MQ=255)
                                                                      aGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATa                              >  2:1668285/1‑100 (MQ=255)
                                                                          aaCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa                          <  2:3893886/100‑1 (MQ=255)
                                                                          aaCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa                          >  2:491870/1‑100 (MQ=37)
                                                                           aCCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat                         >  2:3912245/1‑100 (MQ=255)
                                                                           aCCCTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaat                         >  2:1613662/1‑100 (MQ=37)
                                                                            cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaata                        >  1:2637775/1‑100 (MQ=37)
                                                                            cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaata                        >  1:2734103/1‑100 (MQ=37)
                                                                            cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaa                          >  2:3709274/1‑98 (MQ=37)
                                                                            cccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCAGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGa                           >  2:623534/1‑97 (MQ=37)
                                                                            accTTAAGACTGTGTTGCACAGAATTAAGGTTTTCTTCCCTAGCTGGCGCTTGTCGCCGGCACTCTCAGTAGAATAGCTCTTGAAGGGGCGATAAGaata                        >  2:3633752/2‑100 (MQ=255)
                                                                             ccTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa                       >  1:3604576/1‑100 (MQ=255)
                                                                             ccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa                       >  1:3450678/1‑100 (MQ=37)
                                                                             ccTTAAGCCTGTGTCGCACAGACTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataa                       >  1:3001196/1‑100 (MQ=37)
                                                                             ccTTAAGCCTGTGTCTCACAGACTTAAGGGTTTCTACACCATCAGGCGCTTATCTCCGGCACTGTCAGTGGCTTAGCTCTTGAAGGGTCGATAAGtataa                       >  2:1753101/1‑100 (MQ=25)
                                                                              cTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGaataat                      >  1:3402040/1‑100 (MQ=255)
                                                                                tAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtct                    >  2:3844424/1‑100 (MQ=255)
                                                                                 aaGTCGGTGCGACACAGGCTTAAGGGTTTCTACCACATTCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAAtctc                   <  2:722693/100‑1 (MQ=255)
                                                                                   gTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt                 <  1:3752928/100‑1 (MQ=255)
                                                                                   gTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCAt                 >  2:3168949/1‑100 (MQ=255)
                                                                                      tgtgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATaaa              >  1:1277433/1‑100 (MQ=255)
                                                                                       gtgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAg             >  1:1630386/1‑100 (MQ=255)
                                                                                        tgCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGc            >  2:1995253/1‑100 (MQ=255)
                                                                                            gACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTaa         >  1:1580415/1‑100 (MQ=255)
                                                                                             acacAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAc        <  2:278149/100‑1 (MQ=255)
                                                                                              cacaGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAAcc       <  1:22419/100‑1 (MQ=255)
                                                                                                caGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccg     <  2:557498/100‑1 (MQ=255)
                                                                                                  ggCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc   >  1:2459813/1‑100 (MQ=255)
                                                                                                   gCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgccg  >  1:2584774/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGAAAAAGAAAAAGTTGTATTGAAAATGACTATTTAAGAGATAGGTAAAAAAGTGCAGCGTTCAGAAATAAGAAAACCCTTAAGTCTGTGCGACACAGGCTTAAGGGTTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCG  >  NC_000913/1764603‑1764800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: