Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,400 G→A 17.1% R405W (CGG→TGG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4000GA17.1% 384.1 / 64.0 164R405W (CGG→TGG) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (76/60);  new base A (11/17);  total (87/77)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.02e-01

Only 100 of 167 total aligned reads displayed.

CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140301‑2140499
                                                                                                   |                                                                                                   
cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                                                     >  1:32604/1‑100 (MQ=255)
  gCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                   >  2:3236678/1‑100 (MQ=255)
   cACCGGAGAAATCACCAGCCAGTGACATTTTTCCAGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTg                                                                                                   >  2:1306572/1‑99 (MQ=255)
    aCCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGc                                                                                                 >  2:2087266/1‑100 (MQ=255)
     ccGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGTATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCt                                                                                                >  1:1998684/1‑100 (MQ=255)
      cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTg                                                                                               <  1:2919289/100‑1 (MQ=255)
      cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTg                                                                                               <  2:916/100‑1 (MQ=255)
       ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                                              >  2:3538439/1‑100 (MQ=255)
       ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                                              >  2:1195190/1‑100 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATGGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                          <  1:3843367/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                          <  2:2551322/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                          <  1:3897762/100‑3 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGtt                                                                                         >  2:403279/1‑100 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:3416152/100‑4 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:1828747/100‑4 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  1:379760/100‑4 (MQ=255)
            aaTCACCAACCAGTGACATTTTTCCCGTCAACAAAACCGGAGAGTTAAACGCCGTCAAAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                         <  2:939111/100‑4 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGttt                                                                                        >  1:1240770/1‑100 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGttt                                                                                        >  2:1318890/1‑100 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                        <  1:1123833/100‑5 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                                                       <  2:487987/100‑6 (MQ=255)
                aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATc                                                                                     >  1:639530/1‑100 (MQ=255)
                aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATc                                                                                     <  1:3236700/100‑1 (MQ=255)
                    gccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                                                 >  2:1554228/1‑100 (MQ=255)
                     ccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCgg                                                                                <  1:3154965/100‑1 (MQ=255)
                     ccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCgg                                                                                <  1:854364/100‑1 (MQ=255)
                         tGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAttt                                                                            >  1:2355379/1‑100 (MQ=255)
                          gACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTa                                                                           >  1:2629217/1‑100 (MQ=255)
                           aCATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAt                                                                          <  2:3745166/100‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         >  2:1216648/1‑100 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         <  2:3945721/100‑1 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                         >  1:2761027/1‑100 (MQ=255)
                              tttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGa                                                                       <  1:2504009/100‑1 (MQ=255)
                              tttttCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGa                                                                       <  1:74227/100‑1 (MQ=255)
                                     ggacAACGAAATCGGATAGTTAAACGCCTTCAGGATGGTACCATTCTCAACGTTTTCCAGCCGTGGCTGGGGGTTTATCCGCGGGTTTATTGGTGTcgcg                                                                >  2:780683/4‑100 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                                >  2:2974656/1‑100 (MQ=255)
                                      gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                               <  1:3490915/100‑1 (MQ=255)
                                       tCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTc                                                              >  1:2723187/1‑100 (MQ=255)
                                         aaCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                            <  2:1885442/100‑1 (MQ=255)
                                         aaCGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCa                                                            >  1:3338987/1‑100 (MQ=255)
                                          aCGAACGCGGATAGTTAATCGCCTTCAGAATTGTACCTATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAg                                                           <  2:1895760/100‑1 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                       >  1:2716932/1‑100 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                       <  1:1935444/100‑1 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGTTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   <  2:872324/100‑1 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   <  2:653244/100‑1 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                   >  2:1172397/1‑100 (MQ=255)
                                                  ggATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCAGCGGATTTATTGATGTCGCGTCCAGCGTGcc                                                     >  2:717573/1‑98 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGc                                                 <  2:395615/100‑1 (MQ=255)
                                                     tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                >  2:2355419/1‑100 (MQ=255)
                                                     tAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCa                                                >  2:3258527/1‑100 (MQ=255)
                                                      aGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAg                                               <  2:1240761/100‑1 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              <  2:1404971/100‑1 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              >  1:1181285/1‑100 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                              >  1:1058956/1‑100 (MQ=255)
                                                         tAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGa                                            >  1:932734/1‑100 (MQ=255)
                                                         tAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGa                                            >  2:1515834/1‑100 (MQ=255)
                                                           aaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaaa                                          >  1:3463272/1‑100 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                         <  2:2180047/99‑1 (MQ=255)
                                                               ccTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                      >  1:559690/1‑100 (MQ=255)
                                                               ccTTCAGAATGGTACCAATCTCAACGTGTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                      <  2:1419682/100‑1 (MQ=255)
                                                                  tCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTcc                                   >  1:2458245/1‑100 (MQ=255)
                                                                   cAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCa                                  <  2:1793271/100‑1 (MQ=255)
                                                                   cAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCa                                  <  1:3513976/100‑1 (MQ=255)
                                                                     gAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGCCCAcc                                >  1:595212/1‑100 (MQ=255)
                                                                     gAATGGTAACCATCTCACCGTTTTGCAGCTGTGGCTGGAAGTTTATCCGCGGGTTTATTGGTGTCGTGTCCAGCGTGCCCGCCAGTGAATCATGTcta                                  <  2:1796886/98‑3 (MQ=255)
                                                                         ggTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTa                            >  1:3374952/1‑100 (MQ=255)
                                                                          gTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTa                            >  2:322748/1‑99 (MQ=255)
                                                                           tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                          >  2:2647542/1‑100 (MQ=255)
                                                                               aaTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTg                      <  1:17050/100‑1 (MQ=255)
                                                                                 tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcc                    >  1:2596097/1‑100 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                   <  1:3091961/100‑1 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                   <  2:3668475/100‑1 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                   >  1:3346636/1‑100 (MQ=255)
                                                                                   tcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt                  >  2:881656/1‑100 (MQ=255)
                                                                                   tcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt                  >  1:1311842/1‑100 (MQ=255)
                                                                                     taCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                <  1:3018038/99‑1 (MQ=255)
                                                                                     aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                >  2:835601/1‑100 (MQ=255)
                                                                                     aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc                <  1:2120508/100‑1 (MQ=255)
                                                                                      taaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa               >  2:594203/6‑100 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa               <  1:2033130/100‑1 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa               >  1:889336/1‑100 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa               >  2:2705986/1‑100 (MQ=255)
                                                                                       cGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAg              <  2:3435778/100‑1 (MQ=255)
                                                                                       aaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTCTTGATGTCGCGTCCAGCGTGCACGGCAGTGAAACCTGTCCCCCGTTAAGGTTGCCCTGCa               >  2:3481837/5‑99 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGCTGTCGCGTCCAGCGTGCCTGGTAGTGCAACATGTTCACCGTTAAGTTTGCCCTGCCGt             >  2:2464686/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  2:213323/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:2568973/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:226575/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:1500851/4‑100 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt             >  1:245278/4‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt            >  1:849945/3‑100 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           <  2:1883213/100‑1 (MQ=255)
                                                                                           ttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGACACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           <  1:3743364/99‑1 (MQ=255)
                                                                                           ttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg           >  1:3549544/1‑99 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         <  2:605909/100‑1 (MQ=255)
                                                                                            tCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg         >  1:1500368/1‑100 (MQ=255)
                                                                                             ccAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGt        >  2:357854/1‑100 (MQ=255)
                                                                                              cAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTa       >  1:128342/1‑100 (MQ=255)
                                                                                              cAGCCGTGGCTGGAAGTTTATCCGCGGATGTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTa       <  2:3812745/100‑1 (MQ=255)
                                                                                                   gTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTc  <  1:877176/100‑1 (MQ=255)
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CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAATTTC  >  NC_000913/2140301‑2140499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: