Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,027,058 C→A R178S (CGT→AGT)  fre → NAD(P)H‑flavin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,027,0580CA98.2% 190.2 / NA 56R178S (CGT→AGT) freNAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (29/26);  total (30/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAT  >  NC_000913/4026960‑4027152
                                                                                                  |                                                                                              
aTCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCg                                                                                               >  2:1799081/1‑100 (MQ=255)
    gTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                           <  2:882414/100‑1 (MQ=255)
    gTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                           >  1:1017439/1‑100 (MQ=255)
    gTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                           >  1:399921/1‑100 (MQ=255)
    ccgatctctcTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACt                                                                                           <  1:301750/94‑1 (MQ=255)
       tGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGc                                                                                        >  1:150144/1‑100 (MQ=255)
       tGAGCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGc                                                                                        <  1:1966632/100‑1 (MQ=255)
        gATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGGGTGGGAGTACTGGCa                                                                                       >  1:1986026/1‑100 (MQ=255)
         aTCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCAc                                                                                      <  2:2000807/100‑1 (MQ=255)
         aTCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCAc                                                                                      >  1:339143/1‑100 (MQ=255)
           ctctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACtagatcgg                                                                                    >  2:301751/1‑93 (MQ=255)
           ctctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCg                                                                                    <  2:672894/100‑1 (MQ=255)
             ctGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCgtg                                                                                  >  2:231754/1‑100 (MQ=255)
              tGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCgtgt                                                                                 >  2:1845852/1‑100 (MQ=255)
               gCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGtt                                                                                <  2:1221652/100‑1 (MQ=255)
                 gAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTaa                                                                              >  1:377158/1‑100 (MQ=255)
                       gAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAAcggcgg                                                                        <  2:563619/100‑1 (MQ=255)
                        aGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGt                                                                       >  2:1680361/1‑100 (MQ=255)
                         ggCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTa                                                                      >  2:833686/1‑100 (MQ=255)
                                tCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAgg                                                               <  1:1828207/100‑1 (MQ=255)
                                tCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAgg                                                               <  2:1986014/100‑1 (MQ=255)
                                   ttGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATc                                                            >  2:661031/1‑100 (MQ=255)
                                         cATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTa                                                      <  1:1729312/100‑1 (MQ=255)
                                          aTCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTAc                                                     >  1:959602/1‑100 (MQ=255)
                                             cTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCt                                                  >  2:1998782/1‑100 (MQ=255)
                                                gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                               >  2:1135932/1‑100 (MQ=255)
                                                gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                               <  1:1081674/100‑1 (MQ=255)
                                                gTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGc                                               >  1:1743453/1‑100 (MQ=255)
                                                 tCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCa                                              >  2:284159/1‑100 (MQ=255)
                                                  cTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCag                                             <  2:654648/100‑1 (MQ=255)
                                                  cTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCag                                             <  2:377159/100‑1 (MQ=255)
                                                  cTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCag                                             >  2:1577428/1‑100 (MQ=255)
                                                       aGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCAt                                        <  2:580917/100‑1 (MQ=255)
                                                           gtgCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGata                                    <  1:1918495/100‑1 (MQ=255)
                                                             gCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCagatcggaagagc                                  >  1:1948696/1‑90 (MQ=255)
                                                             gCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATc                                  <  2:1743441/100‑1 (MQ=255)
                                                                 gtggtTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCtata                              <  2:1949626/100‑1 (MQ=255)
                                                                   ggtTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATAtt                            >  1:965777/1‑100 (MQ=255)
                                                                    gtTGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTg                           <  1:70205/100‑1 (MQ=255)
                                                                     ttGAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGc                          >  1:1637988/1‑100 (MQ=255)
                                                                       gAACAACCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCg                        >  1:290230/1‑100 (MQ=255)
                                                                         acaacCGGAAGCGGGCTGGCGTGGGAGTACTGGGACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGGTGGCAGAGCATGATATCTATATTGCCGGa                      >  2:1214361/1‑100 (MQ=255)
                                                                          caacCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGAc                     <  2:339144/100‑1 (MQ=255)
                                                                           aacCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACg                    <  2:32822/100‑1 (MQ=255)
                                                                           aacCGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACg                    >  1:764569/1‑100 (MQ=255)
                                                                             ccGGAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGtt                  >  2:1061424/1‑100 (MQ=255)
                                                                               ggAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGtttt                <  2:399922/100‑1 (MQ=255)
                                                                                gAAGCGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTg               <  2:264748/100‑1 (MQ=255)
                                                                                    cGGGCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGAt           <  2:1736152/100‑1 (MQ=255)
                                                                                      ggCTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATgg         <  1:1404527/100‑1 (MQ=255)
                                                                                        cTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCg       <  2:1017435/100‑1 (MQ=255)
                                                                                        cTGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCg       <  1:1845864/100‑1 (MQ=255)
                                                                                         tGGCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGa      >  2:557791/1‑100 (MQ=255)
                                                                                          ggCGTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGaa     >  1:1057514/1‑100 (MQ=255)
                                                                                             gTGGGAGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAt  >  1:665446/1‑100 (MQ=255)
                                                                                             gTGGGAGTACTGGCACCGTGGTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAt  >  2:1233384/1‑100 (MQ=255)
                                                                                                  |                                                                                              
ATCTGTATGATCTCTGCGAGCTTGAGGCGCTTTCGTTGAAGCATCCTGGTCTGCAAGTGGTGCCGGTGGTTGAACAACCGGAAGCGGGCTGGCGTGGGCGTACTGGCACCGTGTTAACGGCGGTATTGCAGGATCACGGTACGCTGGCAGAGCATGATATCTATATTGCCGGACGTTTTGAGATGGCGAAAAT  >  NC_000913/4026960‑4027152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑