| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,196,331 | C→G | intergenic (‑232/+1) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,196,331 | 0 | C | G | 96.9% | 236.1 / ‑0.6 | 65 | intergenic (‑232/+1) | thiC/rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base G (34/29); total (35/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTC > NC_000913/4196235‑4196424 | cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCa < 2:617621/100‑1 (MQ=255)cccGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCACCGCAAATGGGGAATTGAATGTAAATTACGAGTTAACAGCGCAGAAGTCa > 2:482629/1‑100 (MQ=255) cGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAg > 2:798762/1‑100 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc > 2:1304132/1‑100 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc < 2:1391738/100‑1 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc < 1:1491841/100‑1 (MQ=255) gAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGc > 1:1733547/1‑100 (MQ=255) aaTAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCa > 2:635060/1‑100 (MQ=255) aaaCGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGa > 2:462563/1‑100 (MQ=255) ggTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGtt < 1:806951/100‑1 (MQ=255) gTCTCANCCAGTTAAGGCACTCCGACAAGAAAGTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGNGTTANCAGCGCAGAAGTCAAGCAGGATGttt < 1:1576608/100‑1 (MQ=255) gTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGttt > 2:995840/1‑100 (MQ=255) tctcAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTg < 2:490700/100‑1 (MQ=255) cAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcg < 2:1574832/100‑1 (MQ=255) gCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcgcg > 2:1882082/1‑100 (MQ=255) gCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGAcgcg > 2:225869/1‑100 (MQ=255) ccAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACgcgg > 2:1829329/1‑100 (MQ=255) gTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACgcgggcg < 2:1805031/100‑1 (MQ=255) aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg > 1:317489/1‑100 (MQ=255) aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 2:1855398/100‑1 (MQ=255) aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 1:1286127/100‑1 (MQ=255) aaGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGgcggcg < 1:984138/100‑1 (MQ=255) gCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCa < 1:1599263/100‑1 (MQ=255) aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 1:1304138/100‑1 (MQ=255) aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 2:1296794/100‑1 (MQ=255) aCTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAg < 1:995843/100‑1 (MQ=255) cTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGc < 1:462562/100‑1 (MQ=255) tCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAAGTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCa < 1:1884958/100‑1 (MQ=255) ccGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAc > 2:1971793/1‑100 (MQ=255) ccGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAc < 1:798764/100‑1 (MQ=255) cGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCAcc > 1:250472/1‑100 (MQ=255) gACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACca > 1:1986278/1‑100 (MQ=255) aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag < 1:1238321/100‑1 (MQ=255) aCAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcag < 1:194296/100‑1 (MQ=255) aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca > 2:1049710/1‑100 (MQ=255) aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca < 2:98170/100‑1 (MQ=255) aaGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACcagca > 2:1981089/1‑100 (MQ=255) gAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGa > 2:1046606/1‑100 (MQ=255) ccccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCtt > 2:302414/1‑100 (MQ=255) ccGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTAt < 2:1733535/100‑1 (MQ=255) ggggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGc < 2:953443/100‑1 (MQ=255) gggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGca > 2:1585765/1‑100 (MQ=255) ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcac > 1:498112/1‑100 (MQ=255) ggCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcac > 2:409805/1‑100 (MQ=255) gCATTGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGcaca > 2:263281/1‑100 (MQ=255) ttGAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAg > 1:1230190/1‑100 (MQ=255) gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg < 2:1532116/100‑1 (MQ=255) gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 2:1932153/1‑100 (MQ=255) gAATGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 1:1633713/1‑100 (MQ=255) gAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcg > 1:812738/1‑100 (MQ=255) aTGTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAgcgcg > 1:1122641/1‑100 (MQ=255) gTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt > 2:99944/1‑100 (MQ=255) gTAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCt < 1:350957/100‑1 (MQ=255) tAAATTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCtt > 1:1377070/1‑100 (MQ=255) aaTTACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTcc > 2:344585/1‑100 (MQ=255) tACGCGTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAg > 2:1274219/1‑99 (MQ=255) gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 1:819308/100‑1 (MQ=255) gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 1:765441/100‑1 (MQ=255) gcgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGcc < 2:323706/100‑1 (MQ=255) cgTTAACAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCt > 2:727547/1‑100 (MQ=255) aaCAGCGCAGAAGTCTAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGcc < 1:1981101/100‑1 (MQ=255) aaCAGCGCAGAAGTCAAGCAGGATGTTTGACGCTGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGcc > 2:1313409/1‑100 (MQ=255) aCAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCg < 1:471237/100‑1 (MQ=255) cAGCGCAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGa > 2:1487875/1‑100 (MQ=255) gcAGAAGTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTc > 1:1536512/1‑100 (MQ=255) | CCCGAATAAACGGTCTCAGCCAGTTAAGGCACTCCGACAAGAAATTAGCCCCGCAAATGGGGCATTGAATGTAAATTACGCGTTAACAGCGCAGAACTCAAGCAGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTC > NC_000913/4196235‑4196424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |