Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,866,903 C→T E217K (GAG→AAG)  rpoS ← RNA polymerase, sigma S (sigma 38) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,866,9030CT98.8% 301.8 / ‑4.7 85E217K (GAG→AAG) rpoSRNA polymerase, sigma S (sigma 38) factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (47/37);  total (48/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGTATCTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTCGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAA  >  NC_000913/2866805‑2866999
                                                                                                  |                                                                                                
ggTATCTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTCg                                                                                                 >  1:1925336/1‑100 (MQ=255)
   aTCTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTa                                                                                              <  1:1436121/100‑1 (MQ=255)
     cTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAg                                                                                            >  2:580602/1‑100 (MQ=255)
     cTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAg                                                                                            >  2:878294/1‑100 (MQ=255)
     cTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAg                                                                                            <  2:281719/100‑1 (MQ=255)
     cTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAg                                                                                            >  1:1478888/1‑100 (MQ=255)
     cTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGAGTCTACCGAGGTAATGCGCTTGTTAAg                                                                                            >  2:1924508/1‑100 (MQ=255)
      ttCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGa                                                                                           <  2:490603/100‑1 (MQ=255)
         cGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGa                                                                                        >  2:506512/1‑100 (MQ=255)
          ggACCGTTCTCTTTTTCCTCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGa                                                                                        <  2:938946/99‑1 (MQ=255)
           gACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAg                                                                                      <  2:762111/100‑1 (MQ=255)
           gACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAg                                                                                      <  2:1821751/100‑1 (MQ=255)
            aCCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAg                                                                                      <  2:172398/99‑1 (MQ=255)
            aCCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGc                                                                                     >  2:861752/1‑100 (MQ=255)
            aCCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGc                                                                                     <  2:456361/100‑1 (MQ=255)
             ccGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACACTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCa                                                                                    >  1:530494/1‑100 (MQ=255)
              cGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCAt                                                                                   >  2:1486981/1‑100 (MQ=255)
               gTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATa                                                                                  >  1:874088/1‑100 (MQ=255)
                   tctTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGc                                                                              <  1:1045863/100‑1 (MQ=255)
                   cttccgatctCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGc                                                                              <  1:90141/91‑1 (MQ=255)
                   cttccgatctCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGc                                                                              <  1:1099123/91‑1 (MQ=255)
                    ctTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCt                                                                             >  2:705442/1‑100 (MQ=255)
                      ttttCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGa                                                                           >  1:1110752/1‑100 (MQ=255)
                      ttttCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGa                                                                           >  1:20017/1‑100 (MQ=255)
                         tCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGt                                                                        >  1:806107/1‑100 (MQ=255)
                          cATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGtc                                                                       <  2:1707222/100‑1 (MQ=255)
                          cATCGGCCAGGATGTCCAGCAACGCTTTTTAGGGATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATAGGGCTGACGtc                                                                       >  2:1327056/1‑100 (MQ=255)
                             cGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGcagatcggaag                                                                    >  2:1099118/1‑90 (MQ=255)
                             cGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGcagatcggaag                                                                    >  2:90142/1‑90 (MQ=255)
                             cGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGtcatc                                                                    >  2:35456/1‑100 (MQ=255)
                                 cAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACt                                                                >  2:323724/1‑100 (MQ=255)
                                 cAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACt                                                                >  1:1265610/1‑100 (MQ=255)
                                   ggATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTgg                                                              >  2:102667/1‑100 (MQ=255)
                                     aTGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCt                                                            <  1:1617967/100‑1 (MQ=255)
                                      tGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCtt                                                           >  1:1310132/1‑100 (MQ=255)
                                        tCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTAt                                                         >  2:1795993/1‑100 (MQ=255)
                                        tCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTAt                                                         >  1:1264814/1‑100 (MQ=255)
                                          cAGCAACGCTTTTTTTGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATcc                                                       >  2:1859075/1‑100 (MQ=255)
                                          cAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATcc                                                       >  2:769769/1‑100 (MQ=255)
                                          cAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATcc                                                       >  1:910187/1‑100 (MQ=255)
                                            gCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATcc                                                       <  2:1225597/98‑1 (MQ=255)
                                             cAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGt                                                    <  1:506511/100‑1 (MQ=255)
                                                       tCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGa                                          <  1:1486990/100‑1 (MQ=255)
                                                        cGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGAt                                         >  1:248094/1‑100 (MQ=255)
                                                        cGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGAt                                         <  2:1265604/100‑1 (MQ=255)
                                                         ggAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGAtc                                        <  2:1258736/100‑1 (MQ=255)
                                                             tCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCtt                                    <  1:23149/100‑1 (MQ=255)
                                                              caccacCCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTc                                   >  2:1847417/1‑100 (MQ=255)
                                                                ccaccCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCg                                 >  2:942640/1‑100 (MQ=255)
                                                                ccaccCAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCg                                 >  1:168138/1‑100 (MQ=255)
                                                                   cccAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCAc                              >  2:1220103/1‑100 (MQ=255)
                                                                   cccAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCAc                              >  2:1845193/1‑100 (MQ=255)
                                                                    ccAGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACt                             >  1:432469/1‑100 (MQ=255)
                                                                      aGCGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTg                           <  1:705443/100‑1 (MQ=255)
                                                                        cGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGt                         <  2:1688227/100‑1 (MQ=255)
                                                                        cGGGGTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGt                         <  1:1220108/100‑1 (MQ=255)
                                                                         ggggTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGtt                        >  2:1351235/1‑100 (MQ=255)
                                                                         ggggTGTCTACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGtt                        <  1:193139/100‑1 (MQ=255)
                                                                                 tACCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTcc                <  2:20017/100‑1 (MQ=255)
                                                                                  aCCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCa               >  1:1197603/1‑100 (MQ=255)
                                                                                  aCCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCa               >  2:921431/1‑100 (MQ=255)
                                                                                  aCCGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCa               >  1:1772065/1‑100 (MQ=255)
                                                                                    cttccgatctcgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGc             <  1:745463/90‑1 (MQ=255)
                                                                                    cGAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGc             <  2:1119577/100‑1 (MQ=255)
                                                                                     gAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCt            <  2:1264808/100‑1 (MQ=255)
                                                                                     gAGGTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCt            <  1:323723/100‑1 (MQ=255)
                                                                                        gTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTAt         >  2:816200/1‑100 (MQ=255)
                                                                                        gTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTAt         <  1:1841467/100‑1 (MQ=255)
                                                                                        gTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTAt         <  2:910185/100‑1 (MQ=255)
                                                                                        gTAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTAt         >  1:606772/1‑100 (MQ=255)
                                                                                         tcttGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATg        <  2:606906/97‑1 (MQ=255)
                                                                                         tAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATg        >  2:968533/1‑100 (MQ=255)
                                                                                         tAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATg        >  2:1124691/1‑100 (MQ=255)
                                                                                         tAATGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATg        >  2:1124664/1‑100 (MQ=255)
                                                                                          aaTGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATgg       >  1:214024/1‑100 (MQ=255)
                                                                                           aTGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATggg      <  1:805218/100‑1 (MQ=255)
                                                                                            tGCGCTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATgaga     >  1:606906/1‑97 (MQ=255)
                                                                                             gcgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGAc    <  2:606772/100‑1 (MQ=255)
                                                                                             gcgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGAc    <  2:432470/100‑1 (MQ=255)
                                                                                             gcgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGAc    <  2:874086/100‑1 (MQ=255)
                                                                                             gcgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGAc    <  1:861754/100‑1 (MQ=255)
                                                                                              cgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGcagatcggaag   >  2:745461/1‑90 (MQ=255)
                                                                                              cgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACa   <  1:580602/100‑1 (MQ=255)
                                                                                              cgcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACa   >  1:1331640/1‑100 (MQ=255)
                                                                                               gcTTGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACaa  >  1:771866/1‑100 (MQ=255)
                                                                                                  |                                                                                                
GGTATCTTCCGGACCGTTCTCTTTTTCATCGGCCAGGATGTCCAGCAACGCTTTTTCGGAATCACCACCCAGCGGGGTGTCTACCGAGGTAATGCGCTCGTTAAGACGAAGCATACGGCTGACGTCATCAACTGGCTTATCCAGTTGCTCTGCGATCTCTTCCGCACTTGGTTCATGGTCCAGCTTATGGGACAA  >  NC_000913/2866805‑2866999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑