| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,917,345 | G→A | G763G (GGG→GGA) | barA → | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,917,345 | 0 | G | A | 95.2% | 205.5 / 0.4 | 63 | G763G (GGG→GGA) | barA | hybrid sensory histidine kinase, in two‑component regulatory system with UvrY |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (25/35); total (27/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCG > NC_000913/2917250‑2917439 | cTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGa > 1:757859/1‑100 (MQ=255) tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAg < 1:824684/100‑1 (MQ=255) tCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAg > 1:296033/1‑100 (MQ=255) aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCg > 1:876522/1‑100 (MQ=255) ccaccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAGAGAGAAGCTGCTTGGCGCAGGAATGAGCGAt > 2:1360342/1‑100 (MQ=255) caccaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGAtt > 2:1988863/1‑100 (MQ=255) accaACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTa < 2:33758/100‑1 (MQ=255) cTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCTCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTgg < 1:831657/100‑1 (MQ=255) cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg > 1:1214108/1‑100 (MQ=255) cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg > 1:1757362/1‑100 (MQ=255) ccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGa > 2:479673/1‑100 (MQ=255) aTCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAAcc < 1:798865/100‑1 (MQ=255) cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCg > 2:411154/1‑99 (MQ=255) cAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa > 2:1772092/1‑100 (MQ=255) aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt > 2:20809/1‑100 (MQ=255) aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAt > 1:1390758/1‑100 (MQ=255) caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg < 2:296034/100‑1 (MQ=255) caacaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg < 2:708655/100‑1 (MQ=255) caacaaACGCCGGTTATCGCGGTAACGGAGCATGAAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGAAGGAATGAGCGATTATCTGACGAAACCGAtt < 2:399868/99‑1 (MQ=255) caacaaACGCCGGTTATCGCCGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTg > 2:1236456/1‑100 (MQ=255) acaaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaa > 2:1748011/1‑100 (MQ=255) caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGTCGAAACCGATTgaag > 1:1982343/1‑100 (MQ=255) caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaag < 2:149976/100‑1 (MQ=255) aaCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTgaagaa < 2:134497/100‑1 (MQ=255) aCGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGaagaag < 1:279025/100‑1 (MQ=255) tATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTg < 1:1748023/100‑1 (MQ=255) aTCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGc < 1:1988875/100‑1 (MQ=255) cgcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCAt < 2:1916607/100‑1 (MQ=255) gcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATa < 1:1490481/100‑1 (MQ=255) gcgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATa < 2:1051609/100‑1 (MQ=255) cgGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATaa < 1:1536206/100‑1 (MQ=255) ggTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAt < 2:112609/100‑1 (MQ=255) gTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAAtt > 1:925566/1‑100 (MQ=255) aaCGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg < 2:772309/100‑1 (MQ=255) aCGGCGCATGCAATGGCCGGGCAAAAAGATAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGTAACCGATTGAAGAAGAGCGATTGCATAATttgt < 2:1214103/100‑1 (MQ=255) cGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgtt < 2:1543750/100‑1 (MQ=255) cgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt > 1:1626000/1‑100 (MQ=255) cgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt < 1:1013788/100‑1 (MQ=255) gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg < 1:216650/100‑1 (MQ=255) gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg < 1:1300318/100‑1 (MQ=255) cATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTgc > 2:588923/1‑100 (MQ=255) gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt < 2:1390751/100‑1 (MQ=255) gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCt < 2:757857/100‑1 (MQ=255) gCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGAGCt < 1:1236461/100‑1 (MQ=255) tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACaa < 2:70960/100‑1 (MQ=255) tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACaa > 2:141253/1‑100 (MQ=255) tGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACa > 1:1805815/1‑99 (MQ=255) gCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGc > 2:1867944/1‑100 (MQ=255) ccGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGcc < 1:787563/100‑1 (MQ=255) cGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCt < 1:1715676/100‑1 (MQ=255) gCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGc < 1:20809/100‑1 (MQ=255) aaGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCgg > 2:342861/1‑100 (MQ=255) agagAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGt > 2:455372/1‑100 (MQ=255) gaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAtt < 2:876520/100‑1 (MQ=255) gaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAtt < 2:1982331/100‑1 (MQ=255) aGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTc > 1:1990038/1‑100 (MQ=255) ctgctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCct < 1:479672/100‑1 (MQ=255) gctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct > 1:388584/1‑100 (MQ=255) ctTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCtctc < 2:680973/100‑1 (MQ=255) ggCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgcg > 1:1749759/1‑100 (MQ=255) gcgcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgt < 2:1780011/100‑1 (MQ=255) gcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgtcg > 2:1997544/1‑100 (MQ=255) gcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGcgtcg < 1:588923/100‑1 (MQ=255) | CTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCG > NC_000913/2917250‑2917439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |