| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,427,068 | Δ1 bp | 100% | intergenic (‑35/+8) | alaU ← / ← ileU | tRNA‑Ala/tRNA‑Ile |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,427,068 | 0 | T | . | 50.0% | ‑6.7 / 5.2 | 4 | intergenic (‑35/+8) | alaU/ileU | tRNA‑Ala/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base . (0/2); total (0/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGA > NC_000913/3426977‑3427141 | cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt < 1:2438495/100‑1 (MQ=1)cGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGCACG‑GCAAAttt < 2:2591058/100‑1 (MQ=1) tgtaaaGCAGGCTCTCTCCCAGGTGAGCTATAGCCTCATTACATGTAGTTAGAACCTCTTCAAATTTGC‑CGTGCACCTTTGGTAGGCCTGAGTGGACTTg < 2:3214565/97‑1 (MQ=1) gtgaaaaCCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTAGCAGGGGTGCGCTCTAACCACCTGa < 2:3019782/95‑1 (MQ=0) | CGAACCGCAGACCTCCTGCGTGCAAAGCAGGCGCTCTCCCAGCTGAGCTATAGCCCCATAACATGTAGTTAAAACCTCTTCAAATTTGC‑CGTGCAAATTTGGTAGGCCTGAGTGGACTTGAACCACCGACCTCACCCTTATCAGGGGTGCGCTCTAACCACCTGA > NC_000913/3426977‑3427141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |