Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,213 Δ1 bp 15.2% intergenic (+21/‑222) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2120T.15.2% 456.7 / 80.3 158intergenic (+20/‑223)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base T (80/53);  new base . (9/15);  total (90/68)
Fisher's exact test for biased strand distribution p-value = 4.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

Only 100 of 165 total aligned reads displayed.

ACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTA  >  NC_000913/3888113‑3888311
                                                                                                   |                                                                                                    
aCTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCAt                                                                                                      >  2:1295786/1‑100 (MQ=255)
aCTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCAt                                                                                                      >  2:1317706/1‑100 (MQ=255)
aCTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCGCCAGCTTATGTATTGGTAAGTAACCGCGCTTACAAAGCCGCAt                                                                                                      >  2:3120837/1‑100 (MQ=255)
 cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCAtt                                                                                                     >  1:1583559/1‑100 (MQ=255)
 cTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCAt                                                                                                      <  1:1184724/99‑1 (MQ=255)
  ttAAGCGAAATAACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGAATTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCACATTc                                                                                                    >  2:1653634/1‑100 (MQ=255)
      gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                >  1:806482/1‑100 (MQ=255)
      gCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGAc                                                                                                >  1:1322563/1‑100 (MQ=255)
       cGAAATCACCGGGGAATTTACTTCATACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGTTAAGTAACCGCGCTTACTAAGCCGCATTCTGAc                                                                                                <  2:1471875/99‑1 (MQ=255)
       cGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACt                                                                                               >  1:1608778/1‑100 (MQ=255)
       cGAAATCACCGGGGAATTTACTTCAGACGACCGGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGCAGCCGCATTCTGAc                                                                                                <  2:1216553/99‑1 (MQ=255)
         aaaTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGt                                                                                             >  2:2497969/1‑100 (MQ=255)
          aaTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                            <  2:3598617/100‑1 (MQ=255)
          aaTCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTc                                                                                            <  1:1012483/100‑1 (MQ=255)
             cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAg                                                                                          <  1:3320977/99‑1 (MQ=255)
             cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGa                                                                                        <  1:2380386/100‑1 (MQ=255)
             cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaa                                                                                       <  1:2601210/100‑2 (MQ=255)
                 ggggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                    <  2:1407318/100‑1 (MQ=255)
                 ggggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                    <  1:1961106/100‑1 (MQ=255)
                  gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCgg                                                                                   >  1:1019534/1‑100 (MQ=255)
                  gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                   <  2:2361492/100‑1 (MQ=38)
                  gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                   <  1:523320/100‑1 (MQ=38)
                  gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                   <  1:424760/100‑1 (MQ=38)
                   ggAATTTACTTCAGAGGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                  <  1:1618524/100‑1 (MQ=38)
                   ggAATTTACTTCAGACGACCTGCTGGTGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                  <  2:2314521/100‑1 (MQ=38)
                   ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                  <  1:2323887/100‑1 (MQ=255)
                   ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                  <  1:279968/100‑1 (MQ=255)
                   ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                  <  1:1369740/100‑1 (MQ=38)
                   ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                  <  1:3136156/100‑1 (MQ=38)
                   ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                  <  1:1151031/100‑1 (MQ=38)
                    gAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                 <  2:2765590/100‑1 (MQ=38)
                     aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgctt                                                                                <  2:278420/100‑1 (MQ=255)
                       tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                              <  2:31053/100‑1 (MQ=255)
                        ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACTAAACCGCATTCTGACTGTCAGATGCGgcttcgc                                                                             >  2:2536936/1‑100 (MQ=255)
                         tACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgct                                                                            <  2:3574735/100‑1 (MQ=255)
                         tACTTCAGACGACCTGCTGGCGCGGATTTTCCCCAGCTTCTGTATTGGTAAGTAACCGCTCTTACGAAGCCGCATATGACAGTCAGAATGCGgcttcg                                                                              <  2:2886251/98‑1 (MQ=25)
                          aCTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                           <  2:2158747/100‑1 (MQ=255)
                           cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                          >  1:2602625/1‑100 (MQ=255)
                           cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                          >  1:1989269/1‑100 (MQ=255)
                           cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                          >  2:534732/1‑100 (MQ=255)
                           cTTCAGACGACCTGCTCGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                          <  2:2839434/100‑1 (MQ=255)
                            ttCAGACGACATGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGTCGCATTCTGGCTGTCAGATGCGGCTTCGCTTCa                                                                         <  2:2399033/100‑1 (MQ=255)
                              cAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                       >  1:2406602/1‑100 (MQ=255)
                               aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTg                                                                      <  1:751881/100‑1 (MQ=255)
                               aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTg                                                                      >  1:1423972/1‑100 (MQ=255)
                                gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGt                                                                     <  2:505231/100‑1 (MQ=255)
                                 acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGtt                                                                    <  1:257975/100‑1 (MQ=255)
                                  cgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                   >  2:1837698/1‑100 (MQ=255)
                                    acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                 >  1:2873871/1‑100 (MQ=255)
                                    acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                 <  1:2554057/100‑1 (MQ=255)
                                     ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                >  1:934760/1‑100 (MQ=255)
                                     ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                >  2:1120565/1‑100 (MQ=255)
                                       tgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACt                                                              >  2:2381790/1‑100 (MQ=255)
                                        gctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTc                                                             >  1:901412/1‑100 (MQ=255)
                                            gggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                         <  1:1965899/100‑1 (MQ=255)
                                            gggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                         <  2:1925086/100‑1 (MQ=255)
                                              gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                       >  2:1540276/1‑100 (MQ=255)
                                                ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAtt                                                     >  1:634837/1‑100 (MQ=255)
                                                   ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                  >  1:2232312/1‑100 (MQ=255)
                                                   ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                  <  1:336742/100‑1 (MQ=255)
                                                       ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                              >  2:459422/1‑100 (MQ=255)
                                                       ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                              >  1:2912918/1‑100 (MQ=255)
                                                        tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                             >  1:2554375/1‑100 (MQ=255)
                                                          cAGCTTCAGTAGTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGGTTCATTGTTACCACTCCTGTTATTCCTCAACCCt                                           >  2:1910756/1‑100 (MQ=255)
                                                                 tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTa                                    >  1:1305761/1‑100 (MQ=255)
                                                                   tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                  >  1:1086834/1‑100 (MQ=255)
                                                                   tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                  >  1:3264874/1‑100 (MQ=255)
                                                                    aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTGCCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                 >  1:1626679/1‑100 (MQ=255)
                                                                    aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                 <  2:1719116/100‑1 (MQ=255)
                                                                       ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACAtt                              >  2:3526886/1‑100 (MQ=255)
                                                                       ggTAAGTAACCGCGCTTACAAAGACGAATTCAGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGGTATTCCTCAACCCTTTTTTTaaaa                                 >  2:2202393/1‑96 (MQ=255)
                                                                         taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaa                            >  1:754878/1‑100 (MQ=255)
                                                                           agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                          <  1:2708055/100‑1 (MQ=255)
                                                                           agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                          <  2:1941306/100‑1 (MQ=255)
                                                                             taaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                        >  2:1493952/1‑100 (MQ=255)
                                                                              aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                       <  1:2171503/100‑1 (MQ=255)
                                                                                ccGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTACAATTCtt                     <  1:1916878/100‑1 (MQ=255)
                                                                                ccGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt                     >  1:3417894/1‑100 (MQ=255)
                                                                                  gcgcTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                   >  1:1890404/1‑100 (MQ=255)
                                                                                  gcgcTTACGAAGCCGCATTCTGACTGATAGATGTGGCTTCCCTTCATTATTACCACTCCTATTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                      <  2:3448971/97‑1 (MQ=255)
                                                                                   cgcTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACg                  >  1:1854030/1‑100 (MQ=255)
                                                                                     cTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTa                >  1:2613936/1‑100 (MQ=255)
                                                                                       tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAACATTCTTACGTAAt              >  2:1743951/1‑100 (MQ=255)
                                                                                       tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt              >  1:1411623/1‑100 (MQ=255)
                                                                                       agcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt              >  1:3556698/3‑100 (MQ=37)
                                                                                        gcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt             >  1:2876379/2‑100 (MQ=37)
                                                                                        aCGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt             >  2:3295409/1‑100 (MQ=255)
                                                                                         cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt            >  2:2895086/1‑100 (MQ=255)
                                                                                         cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACCTAAt              >  2:773576/1‑98 (MQ=255)
                                                                                         cGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                   >  2:288864/1‑93 (MQ=37)
                                                                                          gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta           >  1:550884/1‑100 (MQ=255)
                                                                                          gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta           >  2:1863111/1‑100 (MQ=38)
                                                                                           aaGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat          <  1:1787286/100‑1 (MQ=255)
                                                                                           aaGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat          >  2:1423520/1‑100 (MQ=255)
                                                                                            aGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtata         >  1:1621993/1‑100 (MQ=255)
                                                                                               cGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATc      >  1:3316935/1‑100 (MQ=255)
                                                                                                 cATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCtt    >  1:1530686/1‑100 (MQ=255)
                                                                                                  aTTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTACTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCttt   <  2:2090477/100‑1 (MQ=255)
                                                                                                   ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa  <  1:2758021/100‑1 (MQ=255)
                                                                                                   ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa  <  2:3605912/100‑1 (MQ=255)
                                                                                                   |                                                                                                    
ACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTA  >  NC_000913/3888113‑3888311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: