Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,950,966:1 +C 16.3% coding (135/567 nt) yecD → isochorismatase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,950,9651.C16.3% 426.3 / 90.8 129coding (134/567 nt)yecDisochorismatase family protein
Reads supporting (aligned to +/- strand):  ref base . (59/49);  new base C (16/5);  total (75/54)
Fisher's exact test for biased strand distribution p-value = 9.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-02

Only 100 of 143 total aligned reads displayed.

TGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950866‑1951064
                                                                                                    |                                                                                                   
tggtgATGGGTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg                                                                                                      <  2:2936157/100‑1 (MQ=255)
tggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg                                                                                                      <  1:2168936/100‑1 (MQ=255)
 ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgc                                                                                                    <  1:488175/100‑1 (MQ=255)
 ggtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgc                                                                                                    <  1:3013351/100‑1 (MQ=255)
  gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   >  1:3213414/1‑100 (MQ=255)
  gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   <  1:51878/100‑1 (MQ=255)
  gtgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcgcg                                                                                                   <  2:2692594/100‑1 (MQ=255)
   tgATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCgcgc                                                                                                  <  1:2053738/100‑1 (MQ=255)
    gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTcg                                                                                                      <  2:3142088/96‑1 (MQ=255)
    gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGcc                                                                                                 <  2:1388084/100‑1 (MQ=255)
    gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGcc                                                                                                 >  1:2688042/1‑100 (MQ=255)
    gATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGcc                                                                                                 >  1:2532849/1‑100 (MQ=255)
     aTCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTcgccgcca                                                                                               <  2:1370575/100‑6 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  1:3639091/100‑1 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  1:2157209/100‑1 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAg                                                                                               <  2:2742287/100‑1 (MQ=255)
      tCGATTTACAAGAAGGCATCTTACATTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAg                                                                                               <  2:3256564/100‑1 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAg                                                                                               >  2:3085433/1‑99 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGc                                                                                              >  1:176139/1‑100 (MQ=255)
       cGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGc                                                                                              <  1:761526/100‑1 (MQ=255)
         aTTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCgg                                                                                            <  1:1265457/100‑1 (MQ=255)
          tttACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGCGAAATTTCGCCGCCAGcttc                                                                                           <  1:2215176/100‑4 (MQ=38)
           ttACAAGAAGGCGTCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTc                                                                                          >  1:2396829/1‑100 (MQ=255)
            tACAAGAAGGCATCTTACCTTTTGCCGGTGGTCAACAAAATAACGAAGAGGTAGTTAATCGCGACGGGAAGCTGGCGGCGAAATTTCGCGACAGAGGt                                                                                           >  2:240340/1‑98 (MQ=255)
             aCAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAg                                                                                        >  2:789372/1‑100 (MQ=255)
                   agGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtg                                                                                  >  2:145327/1‑100 (MQ=255)
                    ggCCTCTTTCCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtg                                                                                  <  2:2329471/99‑1 (MQ=255)
                    gacATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtgt                                                                                 >  1:2452983/3‑100 (MQ=255)
                     gCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCgtg                                                                                  <  2:2329578/98‑1 (MQ=255)
                      cATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGttt                                                                               <  1:2820426/100‑1 (MQ=255)
                      cATCTTACCTTTTGCCGGTGGTCCACATACTGCAGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCAGTGttt                                                                               >  2:203775/1‑100 (MQ=255)
                         cTTACGTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTg                                                                            <  2:3108564/100‑1 (MQ=255)
                         cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTg                                                                            >  1:3521595/1‑100 (MQ=255)
                         cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTg                                                                            <  1:2960238/100‑1 (MQ=255)
                         cTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTg                                                                            >  1:1227871/1‑100 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCTCATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  2:2093882/100‑1 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           <  1:789377/100‑1 (MQ=255)
                          ttACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTgg                                                                           >  1:1259980/1‑100 (MQ=255)
                             ccTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgc                                                                        <  1:799075/100‑1 (MQ=255)
                             ccTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgc                                                                        >  2:446368/1‑100 (MQ=255)
                             ccTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgc                                                                        <  1:1000491/100‑1 (MQ=255)
                             ccTTTTGCCGGTGGGCCACATAATGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTgc                                                                        <  1:3292267/100‑1 (MQ=255)
                                     cGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGc                                                                <  1:107646/100‑1 (MQ=255)
                                     cGGTGGTCCACACACTGCCGATGAGGTAGTTACACGCGCAGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGc                                                                <  1:2903811/100‑1 (MQ=255)
                                      ggtggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCt                                                               <  1:3431741/100‑1 (MQ=255)
                                       gtggtCGACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCATGTTTCTGGTGCGCGTTGGCTg                                                              <  2:951671/100‑1 (MQ=255)
                                        tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTgg                                                             <  1:1366423/100‑1 (MQ=255)
                                        tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTgg                                                             <  2:176139/100‑1 (MQ=255)
                                        tggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTgg                                                             <  1:2914538/100‑1 (MQ=255)
                                         ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGt                                                            >  2:3332318/1‑100 (MQ=255)
                                         ggtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGt                                                            >  2:1242140/1‑100 (MQ=255)
                                          gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           >  1:3343662/1‑100 (MQ=255)
                                          gtCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTc                                                           >  1:2343708/1‑100 (MQ=255)
                                               caTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCg                                                      <  1:3018208/100‑1 (MQ=255)
                                                aTACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGa                                                     <  2:1005171/100‑1 (MQ=255)
                                                 tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAt                                                    <  1:2730839/100‑1 (MQ=255)
                                                 tACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAt                                                    <  1:2489456/100‑1 (MQ=255)
                                                  aCTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAtt                                                   <  1:2147660/100‑1 (MQ=255)
                                                    tGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTAc                                                 >  2:276295/1‑100 (MQ=255)
                                                     gCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACg                                                >  1:2598062/1‑100 (MQ=255)
                                                      ccGATGAGGTAGTTAATCGCTCCGGGAAGCTGTGGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGAtt                                                   <  2:3211091/96‑1 (MQ=255)
                                                      ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                               <  1:209738/100‑1 (MQ=255)
                                                      ccGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGc                                               >  1:3230571/1‑100 (MQ=255)
                                                        gATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTg                                             >  1:594871/1‑100 (MQ=255)
                                                          tGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGaa                                           >  2:2059445/1‑100 (MQ=255)
                                                           gAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAg                                          <  1:1123137/100‑1 (MQ=255)
                                                            aGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTTTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGc                                         <  2:1956136/100‑1 (MQ=255)
                                                            aGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGc                                         <  1:1517925/100‑1 (MQ=255)
                                                                aGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCCGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTa                                     >  2:545443/1‑100 (MQ=255)
                                                                 gTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaa                                    >  2:1910024/1‑100 (MQ=255)
                                                                  ttAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaa                                   <  1:2102332/100‑1 (MQ=255)
                                                                   tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaaa                                  <  1:339060/100‑1 (MQ=255)
                                                                   tAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGGTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTaaaa                                  >  2:1209970/1‑100 (MQ=255)
                                                                        gcgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGcc                             >  2:1573385/1‑100 (MQ=255)
                                                                         cgcCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCg                            >  2:3578458/1‑100 (MQ=255)
                                                                           gctgaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGt                          >  1:2734730/8‑100 (MQ=37)
                                                                            ctgaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTc                         >  1:1094364/7‑100 (MQ=38)
                                                                              ggAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGa                       >  2:499931/1‑100 (MQ=255)
                                                                              gaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGa                       >  2:511399/5‑100 (MQ=38)
                                                                              gaccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGa                       >  2:2519727/5‑100 (MQ=38)
                                                                               gAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGAt                      >  1:504204/1‑100 (MQ=255)
                                                                                ccgCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATg                     >  1:1620190/3‑100 (MQ=39)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  1:2756589/1‑100 (MQ=255)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  1:416151/1‑100 (MQ=255)
                                                                                  gCTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcg                   >  2:126057/1‑100 (MQ=255)
                                                                                   cTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcgc                  >  2:922761/1‑100 (MQ=255)
                                                                                   cTGGCGCGAAATTTCGCCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATgcgc                  >  2:1353773/1‑100 (MQ=255)
                                                                                    tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                 >  1:281513/1‑100 (MQ=255)
                                                                                    tGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                 <  1:956347/100‑1 (MQ=255)
                                                                                    tGGCGGCGAAATTCCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGcc                 <  1:1910034/100‑1 (MQ=255)
                                                                                      gcggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCt               >  1:3630925/1‑100 (MQ=255)
                                                                                        ggcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa             >  1:2576192/1‑100 (MQ=255)
                                                                                        ggcgAAATTGCGCGCCAGCGGTCAGCTCTTGTTTCTGGTGCTCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCa             <  2:2047336/100‑1 (MQ=255)
                                                                                         gcgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGCTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGCTGCGCCCTCAc            >  2:513732/1‑100 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  2:3430983/1‑100 (MQ=255)
                                                                                          cgAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCAcc           >  1:1961832/1‑100 (MQ=255)
                                                                                               tttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAACACAGCCGGTCGATGCGCCCTCAcc           >  2:2612178/1‑95 (MQ=255)
                                                                                                ttCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaa     >  2:806248/1‑100 (MQ=255)
                                                                                                 tCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTaaa    >  1:96202/1‑100 (MQ=255)
                                                                                                   gcgcCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAgt  <  2:463608/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TGGTGATCGATTTACAAGAAGGCATCTTACCTTTTGCCGGTGGTCCACATACTGCCGATGAGGTAGTTAATCGCGCCGGGAAGCTGGCGGCGAAATTTCGCGCCAGCGGTCAGCCCGTGTTTCTGGTGCGCGTTGGCTGGTCTGCCGATTACGCTGAAGCATTAAAACAGCCGGTCGATGCGCCCTCACCGGCTAAAGT  >  NC_000913/1950866‑1951064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: