Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,991,805:1 +T 15.0% intergenic (+46/‑289) ygeG → / → ygeH SycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,991,8051.T15.0% 521.1 / 96.8 153intergenic (+46/‑289)ygeG/ygeHSycD‑like chaperone family TPR‑repeat‑containing protein/putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (72/58);  new base T (9/14);  total (81/72)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01

Only 100 of 163 total aligned reads displayed.

AAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATC  >  NC_000913/2991706‑2991904
                                                                                                    |                                                                                                    
aaaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc                                                                                                       <  2:628074/100‑1 (MQ=255)
aaaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc                                                                                                       <  1:1697761/100‑1 (MQ=255)
 aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAcc‑‑c                                                                                                    <  2:1442669/100‑1 (MQ=255)
 aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                    <  1:2795832/100‑1 (MQ=255)
 aaGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                    <  1:1319964/100‑1 (MQ=255)
  aGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑Ca                                                                                                   >  2:2277382/1‑100 (MQ=255)
   gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                                  >  2:2644917/1‑100 (MQ=255)
   gcgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAc                                                                                                  <  1:1744768/100‑1 (MQ=255)
    cgcAGGCATATATTGAACTCTTAAACGATTCTTCAGAAGATAATGGCTAAGCATTAAAATAATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAc‑‑c                                                                                                    <  2:535749/97‑1 (MQ=255)
    cgcAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACt                                                                                                 >  1:372144/1‑100 (MQ=255)
      cAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACtat                                                                                               >  2:2144722/1‑100 (MQ=255)
          cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                           <  1:196564/100‑1 (MQ=37)
          cATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAAcc                                                                                           <  1:63091/100‑1 (MQ=37)
            tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                         <  2:2317273/100‑1 (MQ=37)
            tatatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAg                                                                                         <  2:753010/100‑1 (MQ=37)
              tatTGAACTCTTAAACGATAATTCAGAAGATTATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCa                                                                                       <  2:1215234/100‑1 (MQ=25)
              tatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCa                                                                                       >  1:531380/1‑100 (MQ=255)
              tatTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCa                                                                                       <  1:870076/100‑1 (MQ=37)
                ttGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAATCAGCAcc                                                                                     >  2:2517916/1‑100 (MQ=255)
                ttGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAcc                                                                                     >  1:1969102/1‑100 (MQ=255)
                 tGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACct                                                                                    >  2:309757/1‑100 (MQ=255)
                  gAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctc                                                                                   >  1:2519639/1‑100 (MQ=255)
                   aaCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctct                                                                                  <  1:2820079/100‑1 (MQ=255)
                   aaCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACctct                                                                                  <  2:1022508/100‑1 (MQ=255)
                    aCTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCtt                                                                                 >  1:1458608/1‑100 (MQ=255)
                     ctctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTa                                                                                <  2:1441097/100‑1 (MQ=255)
                       ctTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATg                                                                              >  2:2948217/1‑100 (MQ=255)
                       ctTAAACGATAATTCAAAAGCTAATGGTTAAGCAGTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CAGTATAACAAACAACTCTTAt                                                                               >  2:1100248/1‑99 (MQ=255)
                         tAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtt                                                                            >  1:2551130/1‑100 (MQ=255)
                          aaaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGttt                                                                           >  2:284505/1‑100 (MQ=255)
                           aaCGATAATTCAGAGGATGATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtttt                                                                          <  1:3073243/100‑1 (MQ=255)
                           aaCGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGtttt                                                                          >  2:1186002/1‑100 (MQ=255)
                             cttccgatctgaagaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                        <  2:3620733/90‑1 (MQ=255)
                             cGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCt                                                                        <  1:1488312/100‑1 (MQ=255)
                               aTAATTCAGAAGATAATGGCTAAGCCTTACAATCATTTGGTTAAATTTAATGGTTATAGTGCAGATAAC‑‑CACTCTAACCAGCACCTCTTATGTTTTGTTa                                                                      >  2:1733832/1‑100 (MQ=255)
                                tAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTaa                                                                     <  2:790454/100‑1 (MQ=255)
                                   ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATc                                                                  >  1:1378681/1‑100 (MQ=255)
                                   ttCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATc                                                                  <  1:3510731/100‑1 (MQ=255)
                                     cAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAg                                                                <  2:1916187/100‑1 (MQ=255)
                                     cAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAg                                                                <  1:2074828/100‑1 (MQ=255)
                                         agaTAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGAACCTCTTATGTTTTCTTAAATCAGCa                                                              <  2:3297271/98‑1 (MQ=255)
                                            tAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTa                                                         >  1:272705/1‑100 (MQ=255)
                                            tAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTa                                                         >  1:3243098/1‑100 (MQ=255)
                                               tGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTaaaa                                                      <  1:1737678/100‑1 (MQ=255)
                                                ggCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAAc                                                     >  1:1094615/1‑100 (MQ=255)
                                                 gCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACa                                                    >  1:1993283/1‑100 (MQ=255)
                                                  cTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAg                                                   >  2:2041672/1‑100 (MQ=255)
                                                   tAATCATTACAATCACTTGGTTTGAATTGCTGGTTATAGTTCAGCTAAC‑‑CACTATAACCAACACCTCTTCTGTTTTCTTAAATCATCATCTTa                                                         <  2:2498001/93‑1 (MQ=255)
                                                   tAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCAACTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGt                                                  >  2:1457833/1‑100 (MQ=255)
                                                     aGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTa                                                <  1:1374425/100‑1 (MQ=255)
                                                     aGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTa                                                >  1:2403019/1‑100 (MQ=255)
                                                     aGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTa                                                <  2:2090853/100‑1 (MQ=255)
                                                     aGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTa                                                <  1:743548/100‑1 (MQ=255)
                                                         ttACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAagag                                            >  2:2578958/1‑100 (MQ=255)
                                                           aCAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCa                                          >  2:1736125/1‑100 (MQ=255)
                                                            cAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCAt                                         <  1:2640286/100‑1 (MQ=255)
                                                              aTCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATAc                                       <  1:90099/100‑1 (MQ=255)
                                                               tCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACt                                      <  1:3130102/100‑1 (MQ=255)
                                                               tCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACt                                      <  1:2847796/100‑1 (MQ=255)
                                                                    tGGTTAAATTTGCTGGTTATCGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATaaa                                 >  2:2226364/1‑100 (MQ=255)
                                                                     ggTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAAt                                <  2:2003088/100‑1 (MQ=255)
                                                                      gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                               >  2:786117/1‑100 (MQ=255)
                                                                      gTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATc                               >  1:3417091/1‑100 (MQ=255)
                                                                       tcttccgatcTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCa                              <  2:1456150/91‑1 (MQ=25)
                                                                         aaaTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCatt                            <  2:58229/100‑1 (MQ=255)
                                                                           aTTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCACACTATAAATCattat                          >  1:3588966/1‑100 (MQ=255)
                                                                             gtGCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttt                        >  1:1566743/2‑100 (MQ=25)
                                                                               gCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                      <  2:871321/100‑1 (MQ=255)
                                                                               gCTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTAttttt                      >  1:710012/1‑100 (MQ=37)
                                                                                cTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc                     <  2:1760060/100‑1 (MQ=255)
                                                                                cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc                     >  1:1511716/1‑100 (MQ=37)
                                                                                cTGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTc                     >  2:2637307/1‑100 (MQ=37)
                                                                                 tGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa                    >  1:1548816/1‑100 (MQ=37)
                                                                                 tGGTTATAGTG‑‑GTTAACTGCACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCa                    >  1:2524579/1‑100 (MQ=37)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCGGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   <  2:106969/100‑1 (MQ=255)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   >  1:1000250/1‑100 (MQ=255)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   >  2:1499811/1‑100 (MQ=255)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   >  2:435891/1‑100 (MQ=255)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   >  1:2332760/1‑100 (MQ=255)
                                                                                  ggTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCaa                   >  1:3440470/1‑100 (MQ=255)
                                                                                     tataGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGc                >  1:102049/1‑100 (MQ=255)
                                                                                        aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt             <  1:2791495/100‑1 (MQ=255)
                                                                                        aGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGtt             >  1:2137055/1‑100 (MQ=255)
                                                                                         gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa            >  1:237174/1‑100 (MQ=255)
                                                                                         gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa            >  2:2854960/1‑100 (MQ=255)
                                                                                         gTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa            >  1:1831515/1‑100 (MQ=255)
                                                                                          tGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTag           <  2:41042/100‑1 (MQ=255)
                                                                                            cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag         >  1:2632491/1‑100 (MQ=255)
                                                                                            cAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTagag         <  1:1283351/100‑1 (MQ=255)
                                                                                             aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTa            <  2:2804728/96‑1 (MQ=255)
                                                                                             aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAgaga        >  1:1286310/1‑100 (MQ=255)
                                                                                             aGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAgaga        >  1:1381755/1‑100 (MQ=255)
                                                                                              gTTAAC‑‑CACTATAACTATCACTTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGaa       >  2:1319579/1‑100 (MQ=255)
                                                                                              gTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGaa       >  1:1644793/1‑100 (MQ=255)
                                                                                              gTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGaa       >  1:3330718/1‑100 (MQ=255)
                                                                                                tAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACa     >  1:463948/1‑100 (MQ=255)
                                                                                                  aC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCATCATCTTATAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACaa    <  2:434505/99‑1 (MQ=255)
                                                                                                   c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATc  >  1:1446736/1‑100 (MQ=255)
                                                                                                   c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATc  <  1:913539/100‑1 (MQ=255)
                                                                                                   c‑‑cACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATc  >  1:1189076/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
AAAGCGCAGGCATATATTGAACTCTTAAACGATAATTCAGAAGATAATGGCTAAGCATTACAATCATTTGGTTAAATTTGCTGGTTATAGTGCAGTTAAC‑‑CACTATAACCAGCACCTCTTATGTTTTCTTAAATCAGCATCTTAAAACAGTTAAGAGCATACTATAAATCATTATTTTTCAATGCGTTAGAGAACAATC  >  NC_000913/2991706‑2991904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: