Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,379,266:1 (C)7→8 100% coding (732/1128 nt) zapE ← divisome ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,379,2591.C69.0% 271.5 / 134.5 113coding (739/1128 nt)zapEdivisome ATPase
Reads supporting (aligned to +/- strand):  ref base . (11/24);  new base C (29/49);  total (40/73)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355
                                                                                                    |                                                                                                
cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt                                                                                                   >  1:2305846/1‑100 (MQ=255)
cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt                                                                                                   >  1:1168892/1‑100 (MQ=255)
cGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt                                                                                                   >  1:1534226/1‑100 (MQ=255)
 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGGATTTTCTCGttt                                                                                                   >  2:367004/1‑99 (MQ=255)
 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑c                                                                                                 <  1:3086991/100‑1 (MQ=255)
 gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCAAGttt                                                                                                   >  1:2198848/1‑99 (MQ=255)
  cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑cc                                                                                                >  1:967291/1‑100 (MQ=255)
   acaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑ccc                                                                                               <  1:2343307/100‑1 (MQ=255)
    caGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑cccc                                                                                              >  2:263327/1‑100 (MQ=255)
       cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑ccccccc                                                                                           <  1:1710403/100‑1 (MQ=255)
        gtagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑cccccccc                                                                                          >  2:2972571/1‑99 (MQ=255)
         tagtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTGGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGc                                                                                         <  2:569319/100‑1 (MQ=255)
          agtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑ccccccccgc                                                                                        >  1:1748398/1‑97 (MQ=255)
           gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑cccccccc                                                                                          >  2:3415656/1‑96 (MQ=255)
              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgc                                                                                     <  1:1416112/100‑1 (MQ=255)
              aaaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑cccccccccccagc                                                                                    >  1:1978804/1‑96 (MQ=255)
              aaaGAGA‑CACCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcg                                                                                    <  2:2170717/100‑1 (MQ=255)
               aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcg                                                                                    <  1:3248498/100‑1 (MQ=255)
                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTATTTGCCGATGGTTGATTTCTAACGTCGGTGAATTTTTTCGTTTCCCCCCCCGCCAgcg                                                                                    <  2:1675370/98‑1 (MQ=255)
                 gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCa                                                                                 <  2:1534218/100‑1 (MQ=255)
                  agaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCaa                                                                                <  2:850581/100‑1 (MQ=255)
                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCCAc                                                                               >  2:1065/1‑100 (MQ=255)
                   gaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGCCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAAc                                                                               <  2:1948058/100‑1 (MQ=255)
                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAAcc                                                                              >  2:594622/1‑100 (MQ=255)
                     ccGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCa                                                                             >  1:1889939/1‑100 (MQ=255)
                      cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCa                                                                             <  1:468410/100‑1 (MQ=255)
                        ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAg                                                                          <  2:3457377/100‑1 (MQ=255)
                         gatcttCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAg                                                                          <  2:1196261/95‑1 (MQ=255)
                         cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGt                                                                         <  1:1804131/100‑1 (MQ=255)
                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGtt                                                                        <  2:2881756/100‑1 (MQ=255)
                          aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGtt                                                                        <  1:746680/100‑1 (MQ=255)
                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGtn                                                                        <  2:2519648/100‑2 (MQ=255)
                           gCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGttt                                                                       <  1:1568437/100‑1 (MQ=255)
                              tCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAgagatc                                                                     >  1:1196269/1‑95 (MQ=255)
                               cTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAACTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTAt                                                                     >  2:1822942/1‑99 (MQ=255)
                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCa                                                                  <  1:696785/99‑1 (MQ=255)
                                  gTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCAt                                                                 <  1:512739/100‑1 (MQ=255)
                                     ctcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                              <  1:2508102/100‑1 (MQ=255)
                                       cGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCCTCGTTTATCCAttt                                                               >  2:2718705/1‑97 (MQ=255)
                                        gACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                           >  1:2774079/1‑100 (MQ=255)
                                         aCGCCCATCGTTGCTAATGGCCGATGGTTGCTTTCTAACGTCGGTGACTTTCCTTGTTT‑CCCCCCCGCAAGCGCCAACCATAGTTTATCCATTTGCGacc                                                         >  2:379485/1‑100 (MQ=255)
                                          cGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGccc                                                         >  1:1042787/1‑100 (MQ=255)
                                           gCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                          <  2:868674/98‑1 (MQ=255)
                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCgg                                                       >  1:2012854/1‑100 (MQ=255)
                                            cccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCggg                                                      <  1:1882001/100‑1 (MQ=255)
                                             ccATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                     >  2:1589297/1‑100 (MQ=255)
                                               aTTGTTGCTAATGGCCGCTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTTCGCCCGGGtt                                                    <  2:3283059/100‑1 (MQ=255)
                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                    <  1:1064579/100‑1 (MQ=255)
                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                     <  2:1973672/99‑1 (MQ=255)
                                               aTTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGttt                                                   <  2:3248203/100‑1 (MQ=255)
                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTc                                                  <  1:2006778/100‑1 (MQ=255)
                                                 tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                 >  2:2742935/1‑100 (MQ=255)
                                                  gttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                 >  1:2062884/1‑100 (MQ=255)
                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATc                                               >  1:2656140/1‑100 (MQ=255)
                                                    tgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                              >  1:1528843/1‑100 (MQ=255)
                                                     gCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCg                                              >  1:3416421/1‑100 (MQ=255)
                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                             >  1:277117/1‑100 (MQ=255)
                                                      cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                            <  2:1528835/100‑1 (MQ=255)
                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                            >  1:2435107/1‑100 (MQ=255)
                                                       tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTg                                            <  1:1856145/100‑1 (MQ=255)
                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           >  1:287436/1‑100 (MQ=255)
                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           >  1:3020416/1‑100 (MQ=255)
                                                        aaTGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                           <  1:594624/100‑1 (MQ=255)
                                                           ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                       >  2:2693213/1‑100 (MQ=255)
                                                            gCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTg                                       <  2:3322366/100‑1 (MQ=255)
                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      <  2:1526767/100‑1 (MQ=255)
                                                             ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                      >  1:190890/1‑100 (MQ=255)
                                                              cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                    <  1:2501131/100‑1 (MQ=255)
                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                    <  2:2774061/100‑1 (MQ=255)
                                                               gATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCCCCCGGGTTTCATCGTGAAGTg                                       >  2:3582806/1‑97 (MQ=255)
                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                                >  2:1863229/1‑100 (MQ=255)
                                                                   gTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAg                                <  1:1537036/100‑1 (MQ=255)
                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  2:2248300/100‑1 (MQ=255)
                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  2:1173879/100‑1 (MQ=255)
                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                              <  2:721131/100‑1 (MQ=255)
                                                                     tGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGc                               <  2:2147998/99‑1 (MQ=255)
                                                                     tGACTTCTAACGCCGGTGAATTTTCTGTTTG‑CGCCGCCGGCAGCGCCAACCATAGTGTATACATTTGCGCCCGGGTTTCAGCGTGAAGTGGCGCAAGCca                             <  2:1693535/100‑1 (MQ=255)
                                                                       aTTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCcac                            <  2:2839571/100‑1 (MQ=255)
                                                                        tttCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCCTTTGCGCCCGGGTTTCATCGTGAAGTGCCGAAAGCcac                            >  2:3133879/1‑99 (MQ=255)
                                                                           cTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                       <  2:3605800/100‑1 (MQ=255)
                                                                           cTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCAGTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                       <  2:2787419/100‑1 (MQ=255)
                                                                              aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcg                     <  2:3310293/100‑1 (MQ=255)
                                                                               cGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                    >  1:3548174/1‑100 (MQ=255)
                                                                                gTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  2:447527/100‑1 (MQ=255)
                                                                                gTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                   <  2:2415727/100‑1 (MQ=255)
                                                                                  cGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtg                 <  2:287434/100‑1 (MQ=255)
                                                                                  cGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtga                <  1:1101404/100‑1 (MQ=255)
                                                                                    tccgatcttCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag               <  1:130652/93‑1 (MQ=255)
                                                                                    gTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCGGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag               <  2:1782142/100‑1 (MQ=255)
                                                                                    gTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag               <  1:1352407/100‑1 (MQ=255)
                                                                                       aaTTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtga            >  1:2107568/1‑100 (MQ=255)
                                                                                       aaTTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag           <  2:562370/100‑1 (MQ=255)
                                                                                          tttCTCGTTACCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGagtgagtg         <  2:2172675/100‑1 (MQ=255)
                                                                                           ttCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAATGGCGAAAACCACAGATGCCCCTgagtgagttt        >  1:1952853/1‑98 (MQ=255)
                                                                                            tctcGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGTCTGAGTGAGTGTa       <  1:2447001/100‑1 (MQ=255)
                                                                                            tctcGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagagatcgga       >  2:130652/1‑95 (MQ=255)
                                                                                            tctcGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTa       <  1:2972594/100‑1 (MQ=255)
                                                                                             ctcGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg     <  1:1135731/100‑1 (MQ=255)
                                                                                              tcGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg     >  2:1343257/1‑100 (MQ=255)
                                                                                              tcGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACg     <  2:1319566/100‑1 (MQ=255)
                                                                                                    |                                                                                                
CGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTT‑CCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAG  >  NC_000913/3379160‑3379355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑