Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,225:1 +A 15.4% intergenic (+33/‑210) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2241.A15.4% 536.4 / 105.5 162intergenic (+32/‑211)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base . (83/54);  new base A (11/14);  total (94/68)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

Only 100 of 167 total aligned reads displayed.

CACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTT  >  NC_000913/3888126‑3888323
                                                                                                   |                                                                                                   
cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGa                                                                                                    <  1:2380386/100‑1 (MQ=255)
cACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGaa                                                                                                   <  1:2601210/100‑2 (MQ=255)
 aCCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGAt                                                                                                   >  1:1925096/1‑100 (MQ=255)
    ggggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                                >  1:1618018/1‑100 (MQ=255)
    ggggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                                <  2:1407318/100‑1 (MQ=255)
     gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCgg                                                                                               >  1:1019534/1‑100 (MQ=255)
     gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                               <  2:2361492/100‑1 (MQ=38)
     gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                               <  1:523320/100‑1 (MQ=38)
     gggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCgg                                                                                               <  1:424760/100‑1 (MQ=38)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                              <  1:2323887/100‑1 (MQ=255)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                              <  1:279968/100‑1 (MQ=255)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                              <  2:325385/100‑1 (MQ=255)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                              <  1:1369740/100‑1 (MQ=38)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                              <  1:1151031/100‑1 (MQ=38)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                              <  1:3136156/100‑1 (MQ=38)
       gAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                             <  2:2765590/100‑1 (MQ=38)
        aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgctt                                                                                            >  1:1291259/1‑100 (MQ=255)
          tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                          <  2:31053/100‑1 (MQ=255)
          tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcg                                                                                          <  1:1620072/100‑1 (MQ=37)
           ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACTAAACCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                         >  2:2536936/1‑100 (MQ=255)
            tACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgct                                                                                        <  2:3574735/100‑1 (MQ=255)
            tACTTCAGACGACCTGCTGGCGCGGATTTTCCCCAGCTTCTGTATTGGTAAGTAACCGCTCTTACGAAGCCGCATATGACAGTCAGAATGCGgcttcg                                                                                          <  2:2886251/98‑1 (MQ=25)
             ncTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                          >  2:200315/2‑97 (MQ=255)
             aCTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                       <  2:1329062/100‑1 (MQ=255)
             aCTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgctt                                                                                       <  2:2158747/100‑1 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                      >  2:534732/1‑100 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                      >  1:1989269/1‑100 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                      >  1:2602625/1‑100 (MQ=255)
              cTTCAGACGACCTGCTCGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                      <  2:2839434/100‑1 (MQ=255)
                 cAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                   >  1:2406602/1‑100 (MQ=255)
                  aGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTg                                                                                  <  1:751881/100‑1 (MQ=255)
                    acgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGtt                                                                                <  1:257975/100‑1 (MQ=255)
                     cgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                               >  2:1837698/1‑100 (MQ=255)
                      gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                              >  1:3417306/1‑100 (MQ=255)
                       acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                             <  1:2554057/100‑1 (MQ=255)
                       acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                             >  1:2873871/1‑100 (MQ=255)
                         ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCAc                                                                           >  2:2450108/1‑100 (MQ=255)
                           gctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTc                                                                         >  1:901412/1‑100 (MQ=255)
                            ctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                        <  1:1360499/100‑1 (MQ=255)
                               gggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                     <  2:1925086/100‑1 (MQ=255)
                               gggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGt                                                                     <  1:1965899/100‑1 (MQ=255)
                                ggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCCTGACTGTCAGATGCGGCTTCGCTTCAGTGTTACCACTCCTGtt                                                                    >  1:878711/1‑100 (MQ=255)
                                 gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                   >  2:1540276/1‑100 (MQ=255)
                                 gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                   >  1:976561/1‑100 (MQ=255)
                                  cGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                                  <  2:3546378/100‑1 (MQ=255)
                                      ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                              <  1:336742/100‑1 (MQ=255)
                                      ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                              >  1:2232312/1‑100 (MQ=255)
                                       tttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGCTACCACTCCTGTTATTCCTc                                                             <  2:2232298/100‑1 (MQ=255)
                                          ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                          >  1:2912918/1‑100 (MQ=255)
                                          ctcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAc                                                          >  2:459422/1‑100 (MQ=255)
                                           tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                         >  1:2554375/1‑100 (MQ=255)
                                             cAGCTTCAGTAGTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGGTTCATTGTTACCACTCCTGTTATTCCTCAACCCt                                                       >  2:1910756/1‑100 (MQ=255)
                                                  tCTGTATTGGTAAGTAACCGCGCTTACGCAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttttt                                                  >  1:795846/1‑100 (MQ=255)
                                                    tGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTTTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTa                                                >  1:1813292/1‑100 (MQ=255)
                                                     gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                               <  2:2347448/100‑1 (MQ=255)
                                                     gTATTGGTAAGTAACAGCGCTTACGAAGCCGCAATCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAACAATACTGTTATTCCTTCACCCttttttt                                                 >  2:2390863/1‑98 (MQ=255)
                                                      tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaaa                                              >  1:3264874/1‑100 (MQ=255)
                                                       aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTGCCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                             >  1:1626679/1‑100 (MQ=255)
                                                       aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                             <  2:1719116/100‑1 (MQ=255)
                                                           gtaagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTa                                         >  1:641150/1‑100 (MQ=255)
                                                              agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                      <  1:2708055/100‑1 (MQ=255)
                                                              agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                      <  2:1941306/100‑1 (MQ=255)
                                                               ntaaCCGCGCTTACGCAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAt                                     >  2:501720/2‑100 (MQ=255)
                                                                 aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                   <  1:2171503/100‑1 (MQ=255)
                                                                   ccGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTACAATTCtt                                 <  1:1916878/100‑1 (MQ=255)
                                                                   ccGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt                                 <  1:1643189/100‑1 (MQ=255)
                                                                     gcgcTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                               <  2:2798584/100‑1 (MQ=255)
                                                                     gcgcTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTAc                               >  1:1890404/1‑100 (MQ=255)
                                                                     gcgcTTACGAAGCCGCATTCTGACTGATAGATGTGGCTTCCCTTCATTATTACCACTCCTATTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                                  <  2:3448971/97‑1 (MQ=255)
                                                                        cTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTa                            >  1:2613936/1‑100 (MQ=255)
                                                                          tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAACATTCTTACGTAAt                          >  2:1743951/1‑100 (MQ=255)
                                                                          tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                          >  2:1183235/1‑100 (MQ=255)
                                                                          tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                          >  2:2109109/1‑100 (MQ=255)
                                                                          tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                          >  1:1411623/1‑100 (MQ=255)
                                                                          agcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                          >  1:3556698/3‑100 (MQ=37)
                                                                           gcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt                         >  1:2876379/2‑100 (MQ=37)
                                                                           aCGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAtt                         >  2:3295409/1‑100 (MQ=255)
                                                                            cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                        >  2:2895086/1‑100 (MQ=255)
                                                                            cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACCTAAt                          >  2:773576/1‑98 (MQ=255)
                                                                            cGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                        >  2:289037/1‑100 (MQ=37)
                                                                             gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                       >  1:550884/1‑100 (MQ=255)
                                                                             gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                       >  2:1863111/1‑100 (MQ=38)
                                                                             gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                       >  1:1579117/1‑100 (MQ=38)
                                                                              aaGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                      >  2:1423520/1‑100 (MQ=255)
                                                                              aaGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                      <  1:1183243/100‑1 (MQ=255)
                                                                                  cGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATc                  >  1:1063368/1‑100 (MQ=255)
                                                                                  cGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATc                  >  1:3316935/1‑100 (MQ=255)
                                                                                   gCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCt                 >  1:1488376/1‑100 (MQ=255)
                                                                                      ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa              <  1:2758021/100‑1 (MQ=255)
                                                                                      ttCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa              <  2:3605912/100‑1 (MQ=255)
                                                                                       tCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa             <  1:1793052/100‑1 (MQ=255)
                                                                                         tGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaa           >  1:1336528/1‑100 (MQ=255)
                                                                                          gacagTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaa           <  1:5716/96‑1 (MQ=255)
                                                                                          gACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaa          >  2:1225810/1‑100 (MQ=255)
                                                                                           aCTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaaaaaa         <  1:1423528/100‑1 (MQ=255)
                                                                                             tGTCAGATGCGGCTTCGCTTCTTTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg       <  1:1774945/100‑1 (MQ=255)
                                                                                             tGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg       <  1:434680/100‑1 (MQ=255)
                                                                                              gTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc      >  1:470272/1‑100 (MQ=255)
                                                                                                cAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAt    >  1:1134190/1‑100 (MQ=255)
                                                                                                 aGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCAtt   <  2:1626671/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATTT  >  NC_000913/3888126‑3888323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: