Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,520,504:1 +T 17.5% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,520,5041.T17.5% 445.4 / 107.4 143intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
Reads supporting (aligned to +/- strand):  ref base . (69/49);  new base T (12/13);  total (81/62)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.32e-01

Only 100 of 146 total aligned reads displayed.

TTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520409‑4520603
                                                                                                |                                                                                                   
ttttATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGCGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTcc                                                                                                 <  1:3455145/100‑1 (MQ=255)
   tataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                              <  2:3444975/100‑1 (MQ=255)
    ataCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCt                                                                                             <  2:1698542/100‑1 (MQ=38)
     taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                            >  1:3079780/1‑100 (MQ=255)
     taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                            <  2:3339849/100‑1 (MQ=255)
     taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCtt                                                                                            <  1:2027020/100‑1 (MQ=255)
     taCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCtt                                                                                            <  1:4798/100‑1 (MQ=38)
      aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttt                                                                                           <  2:1330498/100‑1 (MQ=255)
      aCAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCttn                                                                                           <  1:3240244/100‑2 (MQ=255)
       cAGTTATTTTATTCTGTTACATGACATAAACCAGCCGACCATTTGCGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGc                                                                                              <  2:3321553/96‑1 (MQ=255)
       cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTTAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  2:2906664/1‑100 (MQ=255)
       cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  1:2191827/1‑100 (MQ=255)
       cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  2:107047/1‑100 (MQ=255)
       cAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTa                                                                                          <  1:3402931/100‑1 (MQ=38)
       cAGATATTTTATTCTTTTACATGAAATAAACCAACATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTATAAAGCCGGAGACATGCTCCGGCTTTa                                                                                          >  2:1017216/1‑100 (MQ=255)
        aGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTAc                                                                                         <  1:243310/100‑1 (MQ=255)
         gATATTTTATTCTTTTACATGACATAAACCACCATACGATTTGAGCGGTCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                        <  2:2461398/100‑1 (MQ=25)
         gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACa                                                                                        >  1:1726526/1‑100 (MQ=255)
         gATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACa                                                                                        <  1:1892695/100‑1 (MQ=38)
          atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                       <  1:3093287/100‑1 (MQ=38)
          atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                       <  1:2946939/100‑1 (MQ=38)
          atatTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGCATGTCTCCGGCTTTACAg                                                                                       <  2:1098705/100‑1 (MQ=38)
            atTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTa                                                                                     >  1:3102914/1‑100 (MQ=255)
             ttttATTCTTTTACCTGAAATAAACCAGCATACCATTTTTGCTATCGCCCGCTGTTGCTCCATACTGTCAAGCCGGAGACATGCTCCTGCTTTACAGTAt                                                                                    >  2:777378/1‑100 (MQ=255)
             ttttATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTAt                                                                                    >  1:262920/1‑100 (MQ=255)
                tATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTAc                                                                                 >  1:1112156/1‑100 (MQ=255)
                  ttCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                               <  1:302076/100‑1 (MQ=255)
                   tCTTTTACCTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCCTACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTga                                                                              >  2:3243788/1‑100 (MQ=255)
                   tCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                               >  1:3361999/1‑99 (MQ=255)
                    cTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTg                                                                               >  2:346789/1‑98 (MQ=255)
                      tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                           >  1:2437231/1‑100 (MQ=255)
                      tttACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGaa                                                                           <  1:1377526/100‑1 (MQ=255)
                       ttACATAAAATAAACCAGCATACCATTTGAGCGAGCGACCGCTGTTCCCCCATACTGTAAAGCCGGAGACATTCTCCGGCTTTACAGTATTACTGAGAAt                                                                          <  2:1065204/100‑1 (MQ=39)
                         aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATac                                                                        >  1:276409/1‑100 (MQ=255)
                         aCATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATac                                                                        >  1:49185/1‑100 (MQ=255)
                           aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATacac                                                                      >  2:1752573/1‑100 (MQ=255)
                           aTGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACAGGCTACGGCCTTACACTATTACTGAGAATaca                                                                       >  2:393325/1‑99 (MQ=255)
                            tGAAATAAACCAGCATACCATTTGAGTGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     >  2:3024862/1‑100 (MQ=255)
                            tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     <  1:3068486/100‑1 (MQ=255)
                            tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     <  1:1734784/100‑1 (MQ=255)
                            tGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACAcc                                                                     <  2:40964/100‑1 (MQ=255)
                               aaTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAt                                                                  >  1:2385953/1‑100 (MQ=255)
                                aTAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATc                                                                 <  1:2259924/100‑1 (MQ=255)
                                  aaaCCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCaa                                                               <  1:3244911/100‑1 (MQ=255)
                                     ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                            <  2:3622547/100‑1 (MQ=255)
                                     ccAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCa                                                            <  2:2095246/100‑1 (MQ=255)
                                        gCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATaa                                                         >  2:1369976/1‑100 (MQ=255)
                                         cATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAAt                                                        >  1:263646/1‑100 (MQ=255)
                                          aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  2:2103714/100‑1 (MQ=255)
                                          aTACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATc                                                       <  2:1123526/100‑1 (MQ=255)
                                             ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                    >  1:3328597/1‑100 (MQ=255)
                                             ccATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGaa                                                    >  1:1622078/1‑100 (MQ=255)
                                               aTTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAgg                                                  >  1:1523919/1‑100 (MQ=255)
                                                  tGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGAAGACATGCTCCGGCTTTACAGTATTACTGAGAATACCCCCATCAAGCATAATCGAAGGTTc                                               <  2:3064338/100‑1 (MQ=255)
                                                     gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCaa                                             >  1:3075243/1‑99 (MQ=255)
                                                     gCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAat                                            >  2:3094302/1‑100 (MQ=255)
                                                        aTCGCCCGCTGTTCCCCCATACTGTACAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATc                                         >  1:2212440/1‑100 (MQ=255)
                                                         tCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCg                                        <  2:276407/100‑1 (MQ=255)
                                                          cGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGc                                       <  2:3404768/100‑1 (MQ=255)
                                                           gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACATTATTACTGAGAATACACCCATCAAGCATAATCTAAGTTTCAATATCGcc                                      <  1:2836733/100‑1 (MQ=255)
                                                           gCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcc                                      >  1:1038678/1‑100 (MQ=255)
                                                             ccGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcccc                                    >  1:440341/1‑100 (MQ=255)
                                                              cGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGcccc                                    >  1:2777304/1‑99 (MQ=255)
                                                                 tGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGATAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCtttt                                <  2:1617857/100‑1 (MQ=255)
                                                                  gtccccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCAACAAGCATAATCGAAGGTTCAATATCGCCCCttttt                               <  1:1339634/97‑1 (MQ=255)
                                                                    tCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                             <  2:728609/100‑1 (MQ=255)
                                                                     cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:909482/1‑100 (MQ=255)
                                                                     cccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            >  1:2809441/1‑100 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGa                             >  2:1650004/1‑98 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGAt                            <  2:1621562/99‑1 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  1:407255/100‑1 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  1:2900702/100‑1 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           >  1:805526/1‑100 (MQ=255)
                                                                      ccccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATc                           <  2:2097622/100‑1 (MQ=255)
                                                                       cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          >  1:3333574/1‑100 (MQ=255)
                                                                       cccATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCg                          <  1:186447/100‑1 (MQ=255)
                                                                             cTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  1:3524380/1‑100 (MQ=255)
                                                                             cTGTAAAGCCGGAGTATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGCTCAATATCCCCCCTTTTTGACCACCAGc                     >  2:716526/1‑99 (MQ=25)
                                                                             cTGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCg                    >  1:656959/1‑100 (MQ=38)
                                                                              tGTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGa                   >  1:694447/1‑100 (MQ=38)
                                                                               gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:2582759/1‑100 (MQ=255)
                                                                               gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:3051447/1‑100 (MQ=255)
                                                                               gTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAt                  >  1:65565/1‑100 (MQ=255)
                                                                               gTAAAGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATAGCCAGCGAt                  >  1:2718260/1‑100 (MQ=38)
                                                                               gTAAAGCCGGAGCATGTCTCCGGCTTTACAATACTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGACCGCCAGCGAt                  >  2:2394062/1‑100 (MQ=37)
                                                                                tAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGAtt                 <  1:22859/100‑1 (MQ=255)
                                                                                 aaaGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  2:939502/1‑100 (MQ=255)
                                                                                 aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  1:3450254/1‑100 (MQ=38)
                                                                                 aaaGCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTc                >  1:1710705/1‑100 (MQ=38)
                                                                                    gCCGGAGCATGTCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTc             >  2:18737/1‑100 (MQ=39)
                                                                                      cGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCAc           >  2:2566566/1‑100 (MQ=255)
                                                                                        gagaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg         <  1:1622454/100‑1 (MQ=255)
                                                                                        ccgatctGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCg         <  1:2552325/94‑1 (MQ=255)
                                                                                          gaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTg       >  2:2732911/1‑100 (MQ=255)
                                                                                          gaCATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTg       >  1:1278354/1‑100 (MQ=255)
                                                                                            cATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCa     >  1:2909360/1‑100 (MQ=255)
                                                                                              tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAg    >  1:3031895/1‑99 (MQ=255)
                                                                                              tGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGa   >  1:1061957/1‑100 (MQ=255)
                                                                                               gCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATGGCCCCTGTTTGATCGCCAGCGATTCGTCACCgagatcgg  >  2:2552309/1‑93 (MQ=255)
                                                                                               gCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAc  >  1:624129/1‑100 (MQ=255)
                                                                                                |                                                                                                   
TTTTATACAGATATTTTATTCTTTTACATGAAATAAACCAGCATACCATTTGAGCGATCGCCCGCTGTTCCCCCATACTGTAAAGCCGGAGACATGCTCCGGCTTTACAGTATTACTGAGAATACACCCATCAAGCATAATCGAAGGTTCAATATCGCCCCTTTTTGATCGCCAGCGATTCGTCACCGTGCAGAC  >  NC_000913/4520409‑4520603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: