Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,316:1 +C 18.2% intergenic (+23/+50) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C18.2% 380.8 / 97.0 121intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (41/58);  new base C (12/10);  total (53/68)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

Only 100 of 129 total aligned reads displayed.

CACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGT  >  NC_000913/922215‑922412
                                                                                                    |                                                                                                  
cACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                     >  1:2499673/1‑100 (MQ=255)
cACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                     <  1:1621729/100‑1 (MQ=255)
cACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAg                                                                                                     <  2:3215727/100‑1 (MQ=255)
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                  >  2:969110/1‑100 (MQ=255)
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                  >  1:1411578/1‑100 (MQ=255)
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGcc                                                                                                  <  1:700294/100‑1 (MQ=255)
  ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGGAATTTTTGAGATAAAAATGCCAGcc                                                                                                  >  2:2582352/1‑100 (MQ=255)
   cGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCg                                                                                                 >  1:766072/1‑100 (MQ=255)
     ggATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGAt                                                                                               >  1:2727528/1‑100 (MQ=255)
      gATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGAt                                                                                               <  1:635036/99‑1 (MQ=255)
       aTTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCg                                                                                             >  1:3130675/1‑100 (MQ=255)
        ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                            <  1:1204484/100‑1 (MQ=255)
          ttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCggc                                                                                           >  1:2722391/1‑99 (MQ=255)
          ttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGc                                                                                          <  1:1719592/100‑1 (MQ=255)
          ttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGc                                                                                          >  2:2785301/1‑100 (MQ=255)
            aTTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATACAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTg                                                                                        <  1:3249999/100‑1 (MQ=255)
             tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                       >  2:72566/1‑100 (MQ=255)
             tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTgg                                                                                       <  2:2731026/100‑1 (MQ=255)
                gggtGGTTGGCCGAACGAAATGCGGCACGGCTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGc                                                                                      <  2:2100189/95‑1 (MQ=255)
                ggCTGGTTACCCGCACGAAATGCGGCACTATTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCa                                                                                     <  2:1607569/99‑1 (MQ=38)
                ggCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAt                                                                                    <  1:982221/100‑1 (MQ=39)
                 gCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtt                                                                                   <  1:3557305/100‑1 (MQ=39)
                  cTGGTTACCCGCAGCATATGCGTCACGACTTGACCCAGTAGATGCTCTGCCACGCCAGTAATTTTTGAGATAATAATCCCAGCCCGATCGGCTGGCAt                                                                                    <  2:2218823/98‑1 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                  >  1:3249164/1‑100 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                  >  2:3397589/1‑100 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                  >  1:803394/1‑100 (MQ=255)
                  cTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAttt                                                                                  <  1:2536271/100‑1 (MQ=255)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                <  1:2698669/100‑1 (MQ=255)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                <  2:3293088/100‑1 (MQ=255)
                    ggTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                <  1:2332621/100‑1 (MQ=255)
                       tACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                             <  1:228066/100‑5 (MQ=38)
                       tACCCGAACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                             <  2:1211819/100‑5 (MQ=37)
                        aCCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatct                                                                            <  1:1206463/100‑6 (MQ=37)
                         cccGCACTAAATGCGGGACGACTGGATCCTGCAAATGCTCTGTCACGAGAGTAATATTCGAGATATAAATGCCAGCGGAGGGGGCTGGCATTTTGc                                                                               >  2:3067075/1‑96 (MQ=255)
                         cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCttt                                                                           <  2:3609550/100‑1 (MQ=255)
                          ccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTa                                                                          >  2:1804980/1‑100 (MQ=255)
                          ccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTa                                                                          <  2:3387179/100‑1 (MQ=255)
                           ctcACGAAATGCGGCACGACTGGATCCATTAGATCCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTa                                                                          <  1:1629192/97‑1 (MQ=255)
                            gCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAgg                                                                        <  2:731034/100‑1 (MQ=255)
                             cACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                       <  1:569958/100‑1 (MQ=255)
                             cACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                       <  1:598328/100‑1 (MQ=255)
                             cACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                       <  1:3152416/100‑1 (MQ=255)
                             cACGAAATGAGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCAAGCCCGATCGGCTGGCAtttttatctcaaaa                                                                       <  2:2425504/100‑11 (MQ=25)
                                gAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGt                                                                    <  1:427685/100‑1 (MQ=255)
                                  aaTGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTac                                                                  <  1:602975/100‑1 (MQ=255)
                                    tGCGGCACGACTGGATCCAGTAGATGCTCCGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                                >  2:2141872/1‑100 (MQ=255)
                                      cGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTAcaca                                                               >  2:624287/1‑99 (MQ=255)
                                       ggCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAAt                                                             <  1:2700560/100‑1 (MQ=255)
                                        gCACGACTGGATACAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCCGCCGATCGGGCTGGCATTTTGCCTTTAGGTCGTACACAATg                                                            >  2:3149609/1‑100 (MQ=255)
                                         cACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATga                                                           >  1:450747/1‑100 (MQ=255)
                                          aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgag                                                          >  1:1005963/1‑100 (MQ=255)
                                          aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgag                                                          <  1:2003969/100‑1 (MQ=255)
                                          aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATgag                                                          <  1:1101523/100‑1 (MQ=255)
                                             aCTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACa                                                       >  1:3102842/1‑100 (MQ=255)
                                                ggATCCAGTAGATGCTGTGGCACGAGAGTAATTTTGGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                     <  1:1794882/99‑1 (MQ=255)
                                                ggATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaa                                                    >  2:1158741/1‑100 (MQ=255)
                                                  aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                  >  1:478007/1‑100 (MQ=255)
                                                  aTCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                  <  2:3632361/100‑1 (MQ=255)
                                                    ccAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGc                                                <  1:3341812/100‑1 (MQ=255)
                                                      aGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGCGAAAGCAGAGCTa                                              >  1:670825/1‑100 (MQ=255)
                                                       gTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAt                                             >  1:1048404/1‑100 (MQ=255)
                                                       gTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAt                                             >  1:1171810/1‑100 (MQ=255)
                                                         aGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAt                                             >  2:364911/1‑98 (MQ=255)
                                                              ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTg                                      <  1:1158750/100‑1 (MQ=255)
                                                              ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTg                                      >  1:1148744/1‑100 (MQ=255)
                                                                ctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGcc                                    >  1:2850236/1‑100 (MQ=255)
                                                                   gCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCt                                 <  2:2789015/100‑1 (MQ=255)
                                                                    cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCtt                                >  2:2641607/1‑100 (MQ=255)
                                                                     aCGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATTCGACTGCCGCTTc                               <  1:2448131/100‑1 (MQ=255)
                                                                     aCGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTc                               <  1:1570707/100‑1 (MQ=255)
                                                                      cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                              <  1:2538238/100‑1 (MQ=255)
                                                                      cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCt                              <  1:3409485/100‑1 (MQ=255)
                                                                       gagaGTAATTGTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATCTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTa                             >  1:459644/1‑100 (MQ=255)
                                                                           gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                         >  1:220364/1‑100 (MQ=255)
                                                                           gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                         >  2:3523356/1‑100 (MQ=255)
                                                                           gTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTGTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                         <  2:1595292/100‑1 (MQ=255)
                                                                            ttccgatctagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCg                        <  1:1766699/91‑1 (MQ=255)
                                                                            tAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCg                        <  1:2558037/100‑1 (MQ=255)
                                                                                 cctaaaggcaaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGCGCTATGCGACTGCCGCTTCTACTTCGACggg                   >  2:40630/10‑100 (MQ=25)
                                                                                  ttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGc                  <  2:1411570/100‑1 (MQ=255)
                                                                                   tGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGca                 <  2:811845/100‑1 (MQ=255)
                                                                                    gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGcac                <  2:1148735/100‑1 (MQ=255)
                                                                                     agaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGagatcggaa               >  2:1766691/1‑92 (MQ=255)
                                                                                       ggcaaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAAt             >  2:2851289/4‑100 (MQ=38)
                                                                                        tAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATa            <  2:2314840/100‑1 (MQ=255)
                                                                                         caaaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa           >  1:701630/2‑100 (MQ=38)
                                                                                           aaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAaca         >  2:326707/1‑100 (MQ=39)
                                                                                            aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac        >  1:1098771/1‑100 (MQ=39)
                                                                                            aaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac        <  1:1674935/100‑1 (MQ=255)
                                                                                            aaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac        <  1:2838761/100‑1 (MQ=255)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTCCCCACTGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCCACGGGCACAATAacac        >  2:3561288/1‑99 (MQ=37)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt       >  2:495451/1‑100 (MQ=39)
                                                                                             aTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACt       >  2:120944/1‑100 (MQ=39)
                                                                                               gCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg     >  1:1448234/1‑100 (MQ=255)
                                                                                               gCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg     >  1:3255324/1‑100 (MQ=255)
                                                                                               gCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCAACGGGCACAATAACACTgg     >  1:2158665/1‑100 (MQ=255)
                                                                                               gCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg     <  1:3111914/100‑1 (MQ=255)
                                                                                                ccAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGc    >  2:1816002/1‑100 (MQ=255)
                                                                                                 cAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg   >  1:326691/1‑100 (MQ=255)
                                                                                                  aGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGt  <  2:96540/100‑1 (MQ=255)
                                                                                                    |                                                                                                  
CACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGT  >  NC_000913/922215‑922412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: