New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4615433 =128 (0.990)12 (0.100) 9/158 NT 9.5% coding (111/864 nt) yjjW putative pyruvate formate lyase activating enzyme
?NC_000913 4615467 = 114 (1.000)coding (77/864 nt) yjjW putative pyruvate formate lyase activating enzyme
Rejected: Frequency below cutoff threshold.

GAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4615541‑4615433
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcaaaaacAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGC  >  NC_000913/4615467‑4615564
                                                                                                                                                                                                               
GAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGC                                                                                                             <  2:3371367/100‑1
  ACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCCGCCACTCGTCTGGCTCCGCTTTTGCAGGGCTGCACTCTGCGCTG                                                                                                           >  1:3580055/1‑100
   CAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGGGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCT                                                                                                            <  2:2657594/98‑1
     GCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAA                                                                                                        >  1:343639/1‑100
      CAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAA                                                                                                        >  1:2335433/1‑99
      CAGATGCGCTTTAGTCAGTAAGATTATCACCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAAGCTGCGCTGCAAA                                                                                                       >  2:2991112/1‑100
        GATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAA                                                                                                     >  1:3528285/1‑100
         ATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC                                                                                                    <  2:2867471/100‑1
         ATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC                                                                                                    <  1:895011/100‑1
                                                                        TCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAACCAGACGACTGCCTGGCCCGTCAACGCAGGAGACGGGGATAATCTTACTGACTAAAGCG                                     >  1:714713/1‑100
                                                                         CTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGC                                    >  1:1851738/1‑100
                                                                          TGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCA                                   >  1:2096645/1‑100
                                                                           GTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCAT                                  >  1:215896/1‑100
                                                                            TTTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTATTGACTAAAGCGCATC                                 >  1:1780707/1‑100
                                                                             TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCT                                >  1:2531211/1‑100
                                                                             TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCT                                >  1:822035/1‑100
                                                                             TTTTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCT                                >  1:2704463/1‑100
                                                                               TTGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGAAGGAGAAGGGGATAATCTTACTGACTAAAGCGCGTCTcc                              >  2:1514794/1‑98
                                                                                TGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCCGGCGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCT                             >  2:1009695/1‑100
                                                                                TGCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCT                             >  1:2414642/1‑100
                                                                                 GCAGGGCTGCAATCTGCGCTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTG                            >  2:2020360/1‑100
                                                                                                   CTGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCG          >  2:477923/1‑100
                                                                                                    TGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGC         >  1:1573686/1‑100
                                                                                                    TGCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGC         >  1:3577125/1‑100
                                                                                                     GCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCG        >  1:1633244/1‑100
                                                                                                     GCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCG        <  2:2659938/100‑1
                                                                                                     GCAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCG        >  1:468842/1‑100
                                                                                                      CAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGG       >  2:1368134/1‑100
                                                                                                      CAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGG       <  1:2097525/100‑1
                                                                                                      CAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGG       >  2:1367825/1‑100
                                                                                                      CAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGG       >  1:430696/1‑100
                                                                                                      CAAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGG       >  1:740469/1‑100
                                                                                                       AAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCCTCTGCTGTTCATGGCTTATCACGCGCGGC      >  2:2163153/1‑100
                                                                                                       AAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGC      <  2:410870/100‑1
                                                                                                       AAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGC      >  1:652203/1‑100
                                                                                                       AAAAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGC      <  1:65614/100‑1
                                                                                                         AAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTG    <  2:3563221/100‑1
                                                                                                         AAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTG    <  1:3536190/100‑1
                                                                                                         AAACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTG    <  1:3520629/100‑1
                                                                                                          AACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGG   <  1:1451820/100‑1
                                                                                                           ACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGC  <  1:2405324/100‑1
                                                                                                           ACAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGC  >  2:1656572/1‑100
                                                                                                            CAGAGCCAGACGACTGCCTGGGCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTAGTCATGGCTTATCACACGCGCCTG    >  2:1331259/1‑97
                                                                                                            CAGAGCCAGACGACTGCCTGGCCCGTCAACGCCGGAGAAGGGGATAATCTTACTGACTACAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGC  >  1:687549/1‑99
                                                                                                                                                                                                               
GAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTTTTTGCAGGGCTGCAATCTGCGCTGCAAAAAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4615541‑4615433
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctgcaaaaacAGAGCCAGACGACTGCCTGGCCCGTCAACGCAGGAGAAGGGGATAATCTTACTGACTAAAGCGCATCTGCTGTTCATGGCTTATCACGCGCGGCTGGC  >  NC_000913/4615467‑4615564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.