BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,926,882	C→A	16.4%	G653V (GGC→GTC)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,882	0	C	A	16.4%	424.2 / 59.5	160	G653V (GGC→GTC)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base C (73/59); new base A (14/12); total (88/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 164 total aligned reads displayed.

TCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCA  >  NC_000913/1926783‑1926981

    cAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATg                                                                                                 <  1:2865087/100‑1 (MQ=255)

      ggCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGc                                                                                               >  1:3544763/1‑100 (MQ=255)

        cGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCt                                                                                             <  2:1694122/100‑1 (MQ=255)

        cGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCt                                                                                             <  2:257262/100‑1 (MQ=255)

         gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                            <  1:2435081/100‑1 (MQ=255)

         gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                            <  2:2684026/100‑1 (MQ=255)

          tAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGa                                                                                           >  2:2445564/1‑100 (MQ=255)

           aGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAg                                                                                          >  1:1834549/1‑100 (MQ=255)

            gCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                         <  1:2248093/100‑1 (MQ=255)

            gCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                         <  2:791564/100‑1 (MQ=255)

              gggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                       >  1:349851/1‑100 (MQ=255)

               ggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                      <  2:1860807/100‑1 (MQ=255)

               ggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATggactcaggcca                                                                                      <  1:786375/100‑11 (MQ=255)

                gTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                     <  2:3491490/100‑1 (MQ=255)

                 tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACAGCCATGGCCTGAGTCCATg                                                                                    >  2:1422777/1‑100 (MQ=255)

                   aTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTc                                                                                  <  1:2097010/100‑1 (MQ=255)

                    tGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                      >  1:121627/1‑95 (MQ=255)

                    tGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCg                                                                                 >  2:1506474/1‑100 (MQ=255)

                     gTTCCCTGCGCCAGCGCGACGAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                <  2:1304963/100‑1 (MQ=255)

                     gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                <  1:3551686/100‑1 (MQ=255)

                     gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCgg                                                                                <  1:3578524/100‑1 (MQ=38)

                      ttCCCTGCGCCAGCGCGACCAGAACAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGt                                                                               <  1:2057516/100‑1 (MQ=255)

                      ttCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGGCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGt                                                                               <  1:1450714/100‑1 (MQ=38)

                      ttCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGt                                                                               <  2:2521373/100‑1 (MQ=38)

                      ttCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATGTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGt                                                                               <  1:80131/100‑1 (MQ=38)

                        cccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                             <  1:1030295/100‑1 (MQ=255)

                        cccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                             >  1:264728/1‑100 (MQ=255)

                         ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                            >  1:2374037/1‑100 (MQ=255)

                         ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                            >  2:1324563/1‑100 (MQ=255)

                         ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaa                                                                            <  2:2920025/100‑3 (MQ=37)

                            gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGCTGTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                          >  2:445057/1‑99 (MQ=255)

                            gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                          >  2:1544915/1‑99 (MQ=255)

                            gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatct                                                                         <  1:246323/100‑6 (MQ=25)

                             cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGc                                                                        >  2:1659705/1‑100 (MQ=255)

                             cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGc                                                                        >  2:386473/1‑100 (MQ=255)

                              gcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCa                                                                       >  2:3607727/1‑100 (MQ=255)

                                cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatctggtc                                                                     <  1:139448/100‑10 (MQ=21)

                                cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatctggt                                                                      <  2:133271/99‑9 (MQ=21)

                                       aCCAGAAAAGCATATTCGATCGCTACGCCTTAGTAAGATTTAAACCGACCAGATTTACCGCCATGGCCTGAGTACATGTGGGTAAAGAGCAATAAAAGAt                                                              >  2:3114137/1‑100 (MQ=255)

                                       aCCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGAt                                                              <  1:386475/100‑1 (MQ=255)

                                       aCCAGAAAAGCAGATTCCATCGCTACGCCTTGGTACGATTTAAAGCGACCAGATGTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGAt                                                              <  2:3542332/100‑1 (MQ=255)

                                         cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                            <  2:3325142/100‑1 (MQ=255)

                                         cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                            >  2:224359/1‑100 (MQ=255)

                                          aGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGt                                                           >  1:1479702/1‑100 (MQ=255)

                                             aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                        >  1:1569033/1‑100 (MQ=255)

                                             aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                        >  1:500609/1‑100 (MQ=255)

                                             aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                        >  2:1893933/1‑100 (MQ=255)

                                             aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                        >  1:3161455/1‑100 (MQ=255)

                                              aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                       <  2:3474311/100‑1 (MQ=255)

                                               aGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCg                                                      >  1:3272113/1‑100 (MQ=255)

                                                gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCgg                                                     <  2:2173734/100‑1 (MQ=255)

                                                gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCgg                                                     >  1:2804302/1‑100 (MQ=255)

                                                 cATATTCCATCGCTACTCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCgg                                                     >  1:1517244/1‑99 (MQ=255)

                                                     ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                <  2:673250/100‑1 (MQ=255)

                                                     ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                <  1:431136/100‑1 (MQ=255)

                                                      tCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                               <  2:242336/100‑1 (MQ=255)

                                                       ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                              >  1:858574/1‑100 (MQ=255)

                                                       ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                              >  1:3149349/1‑100 (MQ=255)

                                                       ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                              <  1:713853/100‑1 (MQ=255)

                                                       ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                              <  1:113297/100‑1 (MQ=255)

                                                        cATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                <  2:232283/97‑1 (MQ=255)

                                                             cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                        >  2:1922004/1‑100 (MQ=255)

                                                              tactgcTTTGTACGATTTAAAGCCACCAGATTTACCGCCATTGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTc                                           <  2:3332119/91‑1 (MQ=255)

                                                              tACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCgcgc                                       <  1:1506482/100‑1 (MQ=255)

                                                                cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCaa                                     >  2:1090175/1‑100 (MQ=255)

                                                                cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCaa                                     >  1:1200919/1‑100 (MQ=255)

                                                                  ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                   >  2:2212995/1‑100 (MQ=255)

                                                                  ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                   <  1:1317950/100‑1 (MQ=255)

                                                                     tCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAg                                <  2:2741600/100‑1 (MQ=255)

                                                                      cGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                               <  1:2357415/100‑1 (MQ=255)

                                                                      cGTACGATTTAAAGCGACCAGATTTACCGCCATGGACTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                               <  1:1592234/100‑1 (MQ=255)

                                                                       gTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                              >  1:2198494/1‑100 (MQ=255)

                                                                        tACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGa                             >  2:2904045/1‑100 (MQ=255)

                                                                         aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                            >  1:1005109/1‑100 (MQ=255)

                                                                         aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                            <  2:2168258/100‑1 (MQ=255)

                                                                         aCGATTTAAAGCGACCAGATGTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                            >  2:1573862/1‑100 (MQ=255)

                                                                            aTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCa                         >  1:3033879/1‑100 (MQ=255)

                                                                                 aaGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTg                    <  2:1002247/100‑1 (MQ=255)

                                                                                   gCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                  <  2:3161432/100‑1 (MQ=255)

                                                                                     gACGAGATTTACCGCCATGGCATGAGTACGTGTCGGTAAAGAGGAATAACAGATGGTAATCGGTTTTCAGCTCGCGCAATTTAGCGATCCCTTTTGCCg                 >  2:737871/1‑99 (MQ=255)

                                                                                     gACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCgg                >  1:768908/1‑100 (MQ=255)

                                                                                      aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCACTTTCGCGACCCAttt                      >  1:3407002/1‑93 (MQ=255)

                                                                                        cAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc             <  2:781215/100‑1 (MQ=255)

                                                                                        cAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc             <  1:1659713/100‑1 (MQ=255)

                                                                                         aGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGGGACCCATTTTGCCGGTTcc            >  2:3061708/1‑100 (MQ=255)

                                                                                          gATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc           <  2:937036/100‑1 (MQ=255)

                                                                                             gtACCGACATGGACTCAGGCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat        >  2:626621/2‑100 (MQ=38)

                                                                                             gtACCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat        >  1:784788/2‑100 (MQ=37)

                                                                                              tACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAata       <  1:945187/100‑1 (MQ=255)

                                                                                               aCCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat      >  1:2232872/1‑100 (MQ=255)

                                                                                               aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat      >  1:663966/1‑100 (MQ=38)

                                                                                                ccGTCATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTGAGCTCGGGCAATTTAGCGACCCATTTTGCCGGTTCCAAATAtt     >  2:2298333/1‑100 (MQ=255)

                                                                                                ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  1:2220216/1‑100 (MQ=255)

                                                                                                ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  2:2846491/1‑100 (MQ=255)

                                                                                                ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  2:2756530/1‑100 (MQ=255)

                                                                                                ccGACATGGACTCAGGCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  1:2749150/1‑100 (MQ=255)

                                                                                                ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  1:2127592/1‑100 (MQ=38)

                                                                                                ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt     >  1:2686999/1‑100 (MQ=38)

                                                                                                  gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc   >  2:3229932/1‑100 (MQ=255)

                                                                                                  gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc   >  1:2415473/1‑100 (MQ=255)

TCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCA  >  NC_000913/1926783‑1926981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`