BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,926,891	G→C	15.9%	S650* (TCA→TGA)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,891	0	G	C	15.9%	439.3 / 74.5	164	S650* (TCA→TGA)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base G (76/62); new base C (15/11); total (91/73)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.86e-01

Only 100 of 168 total aligned reads displayed.

GTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAAT  >  NC_000913/1926792‑1926990

gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                                     <  2:2684026/100‑1 (MQ=255)

gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                                     <  1:1429043/100‑1 (MQ=255)

gTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTg                                                                                                     <  1:2435081/100‑1 (MQ=255)

   gCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                                  <  2:791564/100‑1 (MQ=255)

   gCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGt                                                                                                  <  1:2248093/100‑1 (MQ=255)

     gggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                >  1:349851/1‑100 (MQ=255)

      ggTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCa                                                                                               <  1:3637076/100‑1 (MQ=255)

       gTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                              <  2:3491490/100‑1 (MQ=255)

        tAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACAGCCATGGCCTGAGTCCATg                                                                                             >  2:1422777/1‑100 (MQ=255)

          aTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTc                                                                                           <  2:2787639/100‑1 (MQ=255)

           tGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCa                                                                                               >  1:121627/1‑95 (MQ=255)

           tGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCg                                                                                          >  2:1506474/1‑100 (MQ=255)

            gTTCCCTGCGCCAGCGCGACGAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                         <  2:1304963/100‑1 (MQ=255)

            gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCgg                                                                                         <  1:3551686/100‑1 (MQ=255)

            gTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCgg                                                                                         <  1:3578524/100‑1 (MQ=38)

             ttCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGGCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGt                                                                                        <  1:1450714/100‑1 (MQ=38)

               cccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                                      >  2:2317758/1‑100 (MQ=255)

               cccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                                      >  1:264728/1‑100 (MQ=255)

               cccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTAc                                                                                      <  1:1030295/100‑1 (MQ=255)

                ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                     >  2:1324563/1‑100 (MQ=255)

                ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACa                                                                                     >  1:2374037/1‑100 (MQ=255)

                   gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGCTGTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                                   >  2:445057/1‑99 (MQ=255)

                   gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                                   >  2:1544915/1‑99 (MQ=255)

                   gcgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatct                                                                                  <  1:246323/100‑6 (MQ=25)

                    cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGc                                                                                 >  2:386473/1‑100 (MQ=255)

                    cgcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGc                                                                                 >  2:1659705/1‑100 (MQ=255)

                     gcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCa                                                                                >  2:3607727/1‑100 (MQ=255)

                       cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatctggtc                                                                              <  1:1602415/100‑10 (MQ=21)

                       cAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGACATGGACTCAGGCCATGGCGGTaaatctggt                                                                               <  2:133271/99‑9 (MQ=21)

                           gcgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATaaaa                                                                          <  2:2175090/100‑1 (MQ=255)

                            cgACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAg                                                                         >  1:2206331/1‑100 (MQ=255)

                              aCCAGAAAAGCAGATTCCATCGCTACGCCTTGGTACGATTTAAAGCGACCAGATGTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGAt                                                                       <  2:3542332/100‑1 (MQ=255)

                                cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                                     >  2:224359/1‑100 (MQ=255)

                                cAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATgg                                                                     <  1:1512458/100‑1 (MQ=255)

                                 aGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGt                                                                    >  1:1479702/1‑100 (MQ=255)

                                    aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                 >  2:1893933/1‑100 (MQ=255)

                                    aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                 >  1:3161455/1‑100 (MQ=255)

                                    aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                 >  1:1569033/1‑100 (MQ=255)

                                    aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                 >  1:500609/1‑100 (MQ=255)

                                     aaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                                <  2:3474311/100‑1 (MQ=255)

                                       gCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCgg                                                              >  1:2804302/1‑100 (MQ=255)

                                        cATATTCCATCGCTACTCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCgg                                                              >  1:1517244/1‑99 (MQ=255)

                                            ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                         <  2:673250/100‑1 (MQ=255)

                                            ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                         <  1:431136/100‑1 (MQ=255)

                                            ttCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                         >  2:2529446/1‑100 (MQ=255)

                                             tCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCa                                                        <  2:242336/100‑1 (MQ=255)

                                              ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                                       >  1:858574/1‑100 (MQ=255)

                                              ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                                       <  1:113297/100‑1 (MQ=255)

                                              ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                                       <  1:713853/100‑1 (MQ=255)

                                              ccATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAg                                                       >  1:3149349/1‑100 (MQ=255)

                                               cATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTc                                                         <  2:232283/97‑1 (MQ=255)

                                                    cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                 <  1:950941/100‑1 (MQ=255)

                                                    cTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcgcg                                                 >  2:1922004/1‑100 (MQ=255)

                                                     tactgcTTTGTACGATTTAAAGCCACCAGATTTACCGCCATTGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTc                                                    <  2:3332119/91‑1 (MQ=255)

                                                       cGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCaa                                              >  2:1090175/1‑100 (MQ=255)

                                                         gcgtcatgcGATGTGAAGGGTCCAGAATTACCGGATTGGCGCGAGTCCAGGTAGGTAAAGAGCAATAAAAGATGGTCATCTGTTTTTAGATcgc                                                  <  2:2962671/86‑1 (MQ=255)

                                                         ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                            >  2:332398/1‑100 (MQ=255)

                                                         ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                            >  2:2212995/1‑100 (MQ=255)

                                                         ccTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAtt                                            <  1:1317950/100‑1 (MQ=255)

                                                            tCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAg                                         <  2:2741600/100‑1 (MQ=255)

                                                              gTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCg                                       >  1:2198494/1‑100 (MQ=255)

                                                               tACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGa                                      >  2:2904045/1‑100 (MQ=255)

                                                                aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                                     <  2:2168258/100‑1 (MQ=255)

                                                                aCGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                                     >  1:1005109/1‑100 (MQ=255)

                                                                aCGATTTAAAGCGACCAGATGTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGAc                                     >  2:1573862/1‑100 (MQ=255)

                                                                   aTTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCa                                  >  1:3033879/1‑100 (MQ=255)

                                                                      tAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCAttt                               >  1:1858978/1‑100 (MQ=255)

                                                                          gCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGcc                           <  2:3161432/100‑1 (MQ=255)

                                                                            gACGAGATTTACCGCCATGGCATGAGTACGTGTCGGTAAAGAGGAATAACAGATGGTAATCGGTTTTCAGCTCGCGCAATTTAGCGATCCCTTTTGCCg                          >  2:737871/1‑99 (MQ=255)

                                                                            gACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCgg                         >  1:768908/1‑100 (MQ=255)

                                                                             aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCACTTTCGCGACCCAttt                               >  1:3407002/1‑93 (MQ=255)

                                                                             aCCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGt                        <  1:2356616/100‑1 (MQ=255)

                                                                               cAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc                      <  2:781215/100‑1 (MQ=255)

                                                                               cAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc                      >  1:813768/1‑100 (MQ=255)

                                                                               cAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTc                      <  1:1659713/100‑1 (MQ=255)

                                                                                aGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGGGACCCATTTTGCCGGTTcc                     >  2:3061708/1‑100 (MQ=255)

                                                                                 gATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                    >  1:1209937/1‑100 (MQ=255)

                                                                                 gATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTccc                    <  2:937036/100‑1 (MQ=255)

                                                                                   tttACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCaa                  <  1:795327/100‑1 (MQ=255)

                                                                                    gtACCGACATGGACTCAGGCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAat                 >  2:626621/2‑100 (MQ=38)

                                                                                     tACCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAata                >  1:2090086/1‑100 (MQ=37)

                                                                                      aCCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat               >  1:2232872/1‑100 (MQ=255)

                                                                                      aCCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat               <  1:2752449/100‑1 (MQ=255)

                                                                                      aCCGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGCTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAatat               >  1:776023/1‑100 (MQ=38)

                                                                                       ccGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt              >  2:2846491/1‑100 (MQ=255)

                                                                                       ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt              >  1:2686999/1‑100 (MQ=38)

                                                                                       ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt              >  1:2127592/1‑100 (MQ=38)

                                                                                       ccGACATGGACTCAGGCCATGGCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATAtt              >  2:2484882/1‑100 (MQ=38)

                                                                                        cGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg             <  1:2745487/100‑1 (MQ=255)

                                                                                        cGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg             >  1:51453/1‑100 (MQ=255)

                                                                                         gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc            >  2:3229932/1‑100 (MQ=255)

                                                                                         gCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGc            >  1:2415473/1‑100 (MQ=255)

                                                                                           cATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGATTCCCAATATTGCAc          >  2:1935236/1‑100 (MQ=255)

                                                                                            aTGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCAcc         <  2:1025794/100‑1 (MQ=255)

                                                                                                ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgag     <  1:2077915/100‑1 (MQ=255)

                                                                                                ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgag     <  1:2904413/100‑1 (MQ=255)

                                                                                                ccTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgag     >  2:1669290/1‑100 (MQ=255)

                                                                                                 cTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgaga    <  1:3285292/100‑1 (MQ=255)

                                                                                                 cTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTgaga    >  2:896184/1‑100 (MQ=255)

                                                                                                  tGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGaa   <  1:860027/100‑1 (MQ=255)

GTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAAT  >  NC_000913/1926792‑1926990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`