Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,158 G→C 17.9% intergenic (‑141/+30) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1580GC17.9% 274.3 / 51.2 111intergenic (‑141/+30)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (35/56);  new base C (10/10);  total (45/66)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 115 total aligned reads displayed.

TGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTG  >  NC_000913/2028061‑2028257
                                                                                                 |                                                                                                   
tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                   <  1:294302/100‑1 (MQ=255)
tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                   <  1:1490331/100‑1 (MQ=255)
  gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                 <  1:2145079/100‑1 (MQ=255)
  gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                 <  2:163383/100‑1 (MQ=255)
   cGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATc                                                                                                <  1:912846/100‑1 (MQ=255)
     aTAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCg                                                                                              <  1:3147007/100‑1 (MQ=255)
        gCGTACCGCGAAACGGCGTTAATTTTTTTATAGTTGCATGATATGACGCGACTATGCTTCATTGCTGGCATTAGCTCTCCAGGCCGGATGTGATCCGGc                                                                                            <  2:33177/99‑1 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                           >  1:1323913/1‑100 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                           <  2:597901/100‑1 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                           <  2:1413996/100‑1 (MQ=255)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGTCGGATGTGATCCGGCCt                                                                                          <  1:2638938/100‑1 (MQ=255)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                          <  1:828084/100‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATCATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                          <  1:2858073/99‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                         <  1:2752787/100‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                         <  1:2089463/100‑1 (MQ=255)
           tACCGGAAAACAGCGATAATTTTTTAATGGTGGCATTATAAGACGCGACTATCATTTATTTCAAGCATTATGACACCAGTCCGGATGTGATCCGGCCTGa                                                                                        >  2:2169612/1‑100 (MQ=255)
           tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTgg                                                                                        <  1:1719836/100‑2 (MQ=38)
             ccGCAAAACAGCGATGATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                      <  1:3584592/100‑1 (MQ=255)
             ccGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                      >  1:945901/1‑100 (MQ=255)
              cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                     >  1:2264070/1‑100 (MQ=255)
               gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                    >  1:3620677/1‑100 (MQ=255)
               gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTGgtgtg                                                                                    <  2:3509194/100‑2 (MQ=38)
                cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATGGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                   <  1:2345540/100‑3 (MQ=37)
                 aaaaCAGCGATAATTTTATAGTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTATCATATCAGGCCGGATCACATCCGGCCTGgtgtgct                                                                                  <  2:2539678/100‑4 (MQ=25)
                 aaaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTaa                                                                                  <  2:1290972/100‑1 (MQ=255)
                  aagcAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                 <  2:3521592/97‑1 (MQ=255)
                  aaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGTCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                 <  2:3522654/100‑1 (MQ=255)
                   aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                <  1:1478780/100‑1 (MQ=255)
                     cAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGAt                                                                              >  2:64528/1‑100 (MQ=255)
                      aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATa                                                                             >  1:1176334/1‑100 (MQ=255)
                        cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                           >  1:892124/1‑100 (MQ=255)
                         gATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAccc                                                                          >  1:2106769/1‑100 (MQ=255)
                          aTAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGAATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                           <  2:2125582/98‑1 (MQ=255)
                             aTTTTATAATTGTGGCATGATAATACTCGACTATCATTCATTGCAGGCATTAGCACACCATGCCCGTTGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                      <  2:676013/100‑1 (MQ=255)
                             aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                      <  2:2077265/100‑1 (MQ=255)
                             aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                      <  1:2276271/100‑1 (MQ=255)
                             aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                      <  1:37556/100‑1 (MQ=255)
                              ttttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTca                                                                     <  2:2645340/100‑1 (MQ=255)
                                ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcaca                                                                   <  1:703239/100‑1 (MQ=255)
                                  ataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACAct                                                                 <  1:3118692/100‑1 (MQ=255)
                                   taATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActt                                                                >  2:3200970/1‑100 (MQ=255)
                                    aaTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtc                                                               <  2:2853063/100‑1 (MQ=255)
                                     aTGGTGGCATGATAAGACGCGAGTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtct                                                              >  2:2111246/1‑100 (MQ=255)
                                     aTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtct                                                              >  1:2958615/1‑100 (MQ=255)
                                        gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                           <  2:1236808/100‑1 (MQ=255)
                                        gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                           >  1:3229750/1‑100 (MQ=255)
                                          ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                         <  1:1195384/100‑1 (MQ=255)
                                            cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                       >  2:2914381/1‑100 (MQ=255)
                                            cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                       <  2:347083/100‑1 (MQ=255)
                                             aTGATAAGACGCGACTATCATTCATTGCAGGCAGTAGGACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTGCCGCAGa                                                      <  2:373584/100‑1 (MQ=255)
                                               gagaaGATGCGACTATCATTCATTGCAGCAAATAGCACACCAGGCCGAATGTGATCCGGCCTGATGTTAATGATACCCTTTCGCACTTCTTACGCAGAAc                                                    <  2:500821/97‑1 (MQ=255)
                                               gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAAc                                                    <  2:2685549/100‑1 (MQ=255)
                                                 tAAGACACCACCATCATTCATTGCAGGCATCAGCACACCAGGCCGGACGTGATCCGGCCTGCTGTCAATCACACCCTTTCCCACTCCTTCCGCAAAAc                                                    >  2:2531890/1‑98 (MQ=255)
                                                   aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                >  1:2727742/1‑100 (MQ=255)
                                                     aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                              <  1:1163823/100‑1 (MQ=255)
                                                     aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                              <  2:3468046/100‑1 (MQ=255)
                                                      cgcgACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActccc                                             >  2:3201498/1‑100 (MQ=255)
                                                       gcgACTATCATTCATTGAAGGCATTAGCAAACCTGGCCGAATGTGATACGGCCTGATGTAAATGATCCCCTTTAACACCTCTGCCGCAGAACGAatcccc                                            <  2:536081/100‑1 (MQ=255)
                                                         gACTATCATTCATTGCAGGCAGTAGCGGACCATGCCGGATGTGATGCGTCCTTATGTTAATGATACTCTTTCACTCTTGTTCCGCAGAACGAgtcccc                                            <  2:3022877/98‑1 (MQ=255)
                                                           cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                        >  2:3002263/1‑100 (MQ=255)
                                                           cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                        >  1:2142242/1‑100 (MQ=255)
                                                             aTCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                      >  1:983724/1‑100 (MQ=255)
                                                                attcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGTCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGa                                   >  2:1664032/1‑100 (MQ=255)
                                                                 ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGaa                                  <  2:2620436/100‑1 (MQ=255)
                                                                    attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                               >  1:3548881/1‑100 (MQ=255)
                                                                    attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                               >  1:3006779/1‑100 (MQ=255)
                                                                       gCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCt                            >  1:3145361/1‑100 (MQ=255)
                                                                        cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                           <  2:2443843/100‑1 (MQ=255)
                                                                         aGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                          >  1:2034838/1‑100 (MQ=255)
                                                                             tcattaacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGttt                      >  1:3104257/11‑100 (MQ=25)
                                                                              cattaacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCCCACTTCTTCCGCAAAACGACTCACCTCCCCCGGGACAAGCTCGGCTTc                     >  2:766094/6‑100 (MQ=18)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                    >  2:593376/1‑100 (MQ=255)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                    <  2:1722808/100‑1 (MQ=255)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                    <  2:2005139/100‑1 (MQ=255)
                                                                               attaacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                    >  1:1871840/9‑100 (MQ=25)
                                                                                atctcacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                   <  1:3507589/96‑1 (MQ=255)
                                                                                aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                   <  1:1300853/100‑1 (MQ=255)
                                                                                 taacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  >  1:135666/7‑100 (MQ=37)
                                                                                 gCACACCAGGCCTTATGTGCTCCTTCCTGAGGTTAATTCTATACTTTCACACTTCTTCCGCAGAACGGCTCCCTTCCCCCGGAACAAGCTCGGTTTCATg                  <  2:2529716/100‑1 (MQ=255)
                                                                                 gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  <  2:3266782/100‑1 (MQ=255)
                                                                                 gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  <  1:123935/100‑1 (MQ=255)
                                                                                  aacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGGTTCATGa                 >  1:599650/2‑100 (MQ=37)
                                                                                   acatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTGCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                >  1:3073372/1‑100 (MQ=37)
                                                                                   acatCAGGCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                >  2:851413/1‑100 (MQ=38)
                                                                                   acacCAGTTCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTGCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                  <  2:1490157/98‑1 (MQ=255)
                                                                                   acacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa                 >  2:3141323/1‑99 (MQ=255)
                                                                                    catcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACACGCTCgcattc                     >  2:2475768/4‑89 (MQ=25)
                                                                                    catcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa               >  1:2317180/4‑100 (MQ=37)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAtagat               >  2:3507564/1‑96 (MQ=255)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa               <  2:1925959/100‑1 (MQ=255)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACAATTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa               >  2:861737/1‑100 (MQ=255)
                                                                                      ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg             <  1:3437733/100‑1 (MQ=255)
                                                                                       catgCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTGCCCCCCCCCGGAACAAGCCCGGTAACATGaccagc            >  2:2351632/4‑95 (MQ=255)
                                                                                       cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc            <  2:1292192/100‑1 (MQ=255)
                                                                                         ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt          >  2:564556/1‑100 (MQ=255)
                                                                                          gCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTc         >  2:87982/1‑100 (MQ=255)
                                                                                             ggATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTccc       >  2:3458385/1‑99 (MQ=255)
                                                                                             ggAGGTGATCCGGCCTGATGTTAATGACACCCTTTCACACTTCTTCCGCAGAACAACTCCGCGCCCCCGGAACAAGCTCGGTTTCATGAAAAg             <  2:3387358/93‑1 (MQ=255)
                                                                                                tgtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt   <  1:3266371/100‑1 (MQ=255)
                                                                                                 gtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg  <  2:506360/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
TGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTG  >  NC_000913/2028061‑2028257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: