Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,028,159 T→A 17.9% intergenic (‑142/+29) yedQ ← / ← yodC putative membrane‑anchored diguanylate cyclase/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,028,1590TA17.9% 256.8 / 48.9 111intergenic (‑142/+29)yedQ/yodCputative membrane‑anchored diguanylate cyclase/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (35/56);  new base A (10/10);  total (45/66)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.28e-01

Only 100 of 120 total aligned reads displayed.

TGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGA  >  NC_000913/2028061‑2028258
                                                                                                  |                                                                                                   
tGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGAtgtg                                                                                                    <  1:294302/100‑1 (MQ=255)
  gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                  <  2:163383/100‑1 (MQ=255)
  gCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGAt                                                                                                  <  1:2145079/100‑1 (MQ=255)
     aTAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCg                                                                                               <  1:3147007/100‑1 (MQ=255)
        gCGTACCGCGAAACGGCGTTAATTTTTTTATAGTTGCATGATATGACGCGACTATGCTTCATTGCTGGCATTAGCTCTCCAGGCCGGATGTGATCCGGc                                                                                             <  2:33177/99‑1 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            <  2:597901/100‑1 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            <  2:1413996/100‑1 (MQ=255)
        gCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGcc                                                                                            >  1:1323913/1‑100 (MQ=255)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCt                                                                                           <  2:3546853/100‑1 (MQ=38)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGTCGGATGTGATCCGGCCt                                                                                           <  1:2638938/100‑1 (MQ=255)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                           <  1:828084/100‑1 (MQ=255)
         cGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCt                                                                                           <  1:1395623/100‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTg                                                                                          <  1:2752787/100‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                          <  1:2089463/100‑1 (MQ=255)
          gTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATCACATCCGGCCTg                                                                                          <  2:107747/100‑1 (MQ=255)
           tACCGGAAAACAGCGATAATTTTTTAATGGTGGCATTATAAGACGCGACTATCATTTATTTCAAGCATTATGACACCAGTCCGGATGTGATCCGGCCTGa                                                                                         >  2:2169612/1‑100 (MQ=255)
           tACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTgg                                                                                         <  1:1719836/100‑2 (MQ=38)
             ccGCAAAACAGCGATGATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                       <  1:3584592/100‑1 (MQ=255)
             ccGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATg                                                                                       >  1:945901/1‑100 (MQ=255)
              cGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGt                                                                                      >  1:2264070/1‑100 (MQ=255)
               gCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGtt                                                                                     >  1:3620677/1‑100 (MQ=255)
                cAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATGGCAGGCATTAGCACATCAGGCCGGATCACATCCGGCCTGgtgtgc                                                                                    <  1:2345540/100‑3 (MQ=37)
                 aaaaCAGCGATAATTTTATAGTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTATCATATCAGGCCGGATCACATCCGGCCTGgtgtgct                                                                                   <  2:2539678/100‑4 (MQ=25)
                  aagcAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                  <  2:3521592/97‑1 (MQ=255)
                  aaaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGTCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAAt                                                                                  <  2:3522654/100‑1 (MQ=255)
                   aaCAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATg                                                                                 <  1:1478780/100‑1 (MQ=255)
                      aGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATa                                                                              >  1:1176334/1‑100 (MQ=255)
                        cGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                            >  1:892124/1‑100 (MQ=255)
                          aTAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGAATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATAcc                                                                            <  2:2125582/98‑1 (MQ=255)
                             aTTTTATAATTGTGGCATGATAATACTCGACTATCATTCATTGCAGGCATTAGCACACCATGCCCGTTGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                       <  2:676013/100‑1 (MQ=255)
                             aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                       <  2:2077265/100‑1 (MQ=255)
                             aTTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTc                                                                       <  1:2276271/100‑1 (MQ=255)
                               tttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcac                                                                     <  1:435700/100‑1 (MQ=255)
                                ttATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTcaca                                                                    <  1:703239/100‑1 (MQ=255)
                                  ataATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACAct                                                                  <  1:3118692/100‑1 (MQ=255)
                                   taATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActt                                                                 >  2:3200970/1‑100 (MQ=255)
                                    aaTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtc                                                                <  2:2853063/100‑1 (MQ=255)
                                     aTGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACActtct                                                               >  1:2958615/1‑100 (MQ=255)
                                       ggtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGCTTGTGATCCGGCCTGATGTTAATGATACCCTTTCACACtgcttc                                                             <  2:1720985/100‑1 (MQ=255)
                                        gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                            <  2:1236808/100‑1 (MQ=255)
                                        gtggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTcc                                                            >  1:3229750/1‑100 (MQ=255)
                                          ggCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGc                                                          <  1:1195384/100‑1 (MQ=255)
                                            cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                        <  2:347083/100‑1 (MQ=255)
                                            cATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAg                                                        >  2:2914381/1‑100 (MQ=255)
                                             aTGATAAGACGCGACTATCATTCATTGCAGGCAGTAGGACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTGCCGCAGa                                                       <  2:373584/100‑1 (MQ=255)
                                               gagaaGATGCGACTATCATTCATTGCAGCAAATAGCACACCAGGCCGAATGTGATCCGGCCTGATGTTAATGATACCCTTTCGCACTTCTTACGCAGAAc                                                     <  2:500821/97‑1 (MQ=255)
                                               gATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAAc                                                     <  2:2685549/100‑1 (MQ=255)
                                                 tAAGACACCACCATCATTCATTGCAGGCATCAGCACACCAGGCCGGACGTGATCCGGCCTGCTGTCAATCACACCCTTTCCCACTCCTTCCGCAAAAc                                                     >  2:2531890/1‑98 (MQ=255)
                                                   aGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGAct                                                 >  1:2727742/1‑100 (MQ=255)
                                                     aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                               <  1:1163823/100‑1 (MQ=255)
                                                     aCGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcc                                               <  2:3468046/100‑1 (MQ=255)
                                                      cgcgACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActccc                                              >  2:3201498/1‑100 (MQ=255)
                                                       gcgACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccc                                             <  2:601596/100‑1 (MQ=255)
                                                       gcgACTATCATTCATTGAAGGCATTAGCAAACCTGGCCGAATGTGATACGGCCTGATGTAAATGATCCCCTTTAACACCTCTGCCGCAGAACGAatcccc                                             <  2:536081/100‑1 (MQ=255)
                                                         gACTATCATTCATTGCAGGCAGTAGCGGACCATGCCGGATGTGATGCGTCCTTATGTTAATGATACTCTTTCACTCTTGTTCCGCAGAACGAgtcccc                                             <  2:3022877/98‑1 (MQ=255)
                                                           cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                         >  2:3002263/1‑100 (MQ=255)
                                                           cTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGActcccctccc                                         >  1:2142242/1‑100 (MQ=255)
                                                             aTCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTccccc                                       >  1:983724/1‑100 (MQ=255)
                                                                 ttcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGaa                                   <  2:2620436/100‑1 (MQ=255)
                                                                  tcattGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAAc                                  <  1:3029390/100‑1 (MQ=255)
                                                                    attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                                >  1:3006779/1‑100 (MQ=255)
                                                                    attGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACaa                                >  1:3548881/1‑100 (MQ=255)
                                                                       gCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCt                             >  1:3145361/1‑100 (MQ=255)
                                                                        cAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTc                            <  2:2443843/100‑1 (MQ=255)
                                                                         aGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCg                           >  1:2034838/1‑100 (MQ=255)
                                                                              cattaacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCCCACTTCTTCCGCAAAACGACTCACCTCCCCCGGGACAAGCTCGGCTTc                      >  2:766094/6‑100 (MQ=18)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     >  2:593376/1‑100 (MQ=255)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     <  2:2005139/100‑1 (MQ=255)
                                                                               tAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     <  2:1722808/100‑1 (MQ=255)
                                                                               attaacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCa                     >  1:1871840/9‑100 (MQ=25)
                                                                                atctcacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    <  1:3507589/96‑1 (MQ=255)
                                                                                aGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAt                    >  1:2132234/1‑100 (MQ=255)
                                                                                 taacatcAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   >  1:135666/7‑100 (MQ=37)
                                                                                 gCACACCAGGCCTTATGTGCTCCTTCCTGAGGTTAATTCTATACTTTCACACTTCTTCCGCAGAACGGCTCCCTTCCCCCGGAACAAGCTCGGTTTCATg                   <  2:2529716/100‑1 (MQ=255)
                                                                                 gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATCCCCTTTAACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   >  2:2598669/1‑100 (MQ=255)
                                                                                 gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   <  1:123935/100‑1 (MQ=255)
                                                                                 gCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   <  2:3266782/100‑1 (MQ=255)
                                                                                  aacatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGGTTCATGa                  >  1:599650/2‑100 (MQ=37)
                                                                                   acatCAGGCCGGATCACATCCGGCCTGGTGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTGCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                 >  1:3073372/1‑100 (MQ=37)
                                                                                   acatCAGGCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaa                 >  2:851413/1‑100 (MQ=38)
                                                                                   acacCAGTTCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTGCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATg                   <  2:1490157/98‑1 (MQ=255)
                                                                                   acacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGa                  >  2:3141323/1‑99 (MQ=255)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCAtagat                >  2:3507564/1‑96 (MQ=255)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                <  2:1925959/100‑1 (MQ=255)
                                                                                    cacCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACAATTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGaaa                >  2:861737/1‑100 (MQ=255)
                                                                                      ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg              >  2:3454384/1‑100 (MQ=255)
                                                                                      ccAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAg              <  1:3437733/100‑1 (MQ=255)
                                                                                       catgCCGGATCACATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTGCCCCCCCCCGGAACAAGCCCGGTAACATGaccagc             >  2:2351632/4‑95 (MQ=255)
                                                                                       cAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGc             <  2:1292192/100‑1 (MQ=255)
                                                                                         ggCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCtt           >  2:564556/1‑100 (MQ=255)
                                                                                          gCCGTATGTTATCCGTCTCTATGGTAATGATACCCTGTCACACTTCTTCCTCATAATGACTCCCCTCCCCCTTAACAAGCTCGGTTTCTTGa                  <  2:2166042/92‑1 (MQ=255)
                                                                                          gCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTc          >  2:87982/1‑100 (MQ=255)
                                                                                           ccGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTcc         <  1:2372997/100‑1 (MQ=255)
                                                                                             ggATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTccc        >  2:3458385/1‑99 (MQ=255)
                                                                                                tgtgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCtt    <  1:3266371/100‑1 (MQ=255)
                                                                                                 ttgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTg   <  2:136320/99‑1 (MQ=255)
                                                                                                  tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa  >  1:1002305/1‑100 (MQ=255)
                                                                                                  tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa  <  2:1018056/100‑1 (MQ=255)
                                                                                                  tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGa  >  1:136320/1‑100 (MQ=255)
                                                                                                  tgATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTAACGCTTGa  >  1:175694/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGGCGATAGCGTACCGCAAAACAGCGATAATTTTATAATGGTGGCATGATAAGACGCGACTATCATTCATTGCAGGCATTAGCACACCAGGCCGGATGTGATCCGGCCTGATGTTAATGATACCCTTTCACACTTCTTCCGCAGAACGACTCCCCTCCCCCGGAACAAGCTCGGTTTCATGAAAAGCTTCCCGCTTGA  >  NC_000913/2028061‑2028258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: