Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,140,400 G→A 20.5% R405W (CGG→TGG)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,140,4000GA20.5% 298.2 / 69.4 146R405W (CGG→TGG) asmAsuppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (48/68);  new base A (13/17);  total (61/85)
Fisher's exact test for biased strand distribution p-value = 8.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01

Only 100 of 147 total aligned reads displayed.

CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAAT  >  NC_000913/2140301‑2140496
                                                                                                   |                                                                                                
cAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCg                                                                                                  >  1:2861875/1‑100 (MQ=255)
     ccGGAGAAATCACCAGCCAGTGACATTTTTCCGGTTAATGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGc                                                                                              <  2:45207/99‑1 (MQ=255)
      cGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTg                                                                                            <  2:166907/100‑1 (MQ=255)
       ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGCATGGTACCAATCTCAACGTTTTCCAGCCGTGGCt                                                                                             >  2:484809/1‑98 (MQ=255)
       ggAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTg                                                                                            >  2:104819/1‑99 (MQ=255)
       gaagaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTgg                                                                                           <  2:2643036/98‑1 (MQ=255)
        gagaAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTgg                                                                                           <  1:2499552/99‑1 (MQ=255)
           aaaTCACCAGCCTGTTACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCTTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                       <  2:2454727/100‑3 (MQ=255)
           aaaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaa                                                                                       <  1:2501381/100‑3 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaac                                                                                      <  1:2123951/100‑4 (MQ=255)
            aaTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATCGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGa                                                                                          >  2:1181222/1‑96 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                     <  1:44911/100‑5 (MQ=255)
             aTCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacg                                                                                     <  1:2951902/100‑5 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                                                    <  1:2232762/100‑6 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgt                                                                                    <  1:2720723/100‑6 (MQ=255)
              tCACCAGCCAGTGACATTTTTCCGGTCAACGAAAGCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTa                                                                                    <  1:117263/100‑1 (MQ=255)
                aCCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCACGGCTGGaaaacgttg                                                                                  <  1:114912/100‑8 (MQ=255)
                 ccagccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATcc                                                                                 >  1:772634/1‑100 (MQ=255)
                   agccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgc                                                                               <  2:1874271/100‑1 (MQ=255)
                    gccagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCcgcg                                                                              <  1:688049/100‑1 (MQ=255)
                      cagTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGa                                                                            >  1:3320255/1‑100 (MQ=255)
                       agTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAt                                                                           <  1:2034460/100‑1 (MQ=255)
                       agTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAt                                                                           <  2:1961855/100‑1 (MQ=255)
                        gtcacgtttttCCGCTAAACGAAGTCGCTTGGTTAAACGCCTTAAGAGTGGTACCAAGCTCAACGTTTTCCAGTTGTGGTTGGAAGTTTATCCGCGGAt                                                                           <  2:1892776/93‑1 (MQ=255)
                        gTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGAtt                                                                          >  2:230616/1‑100 (MQ=255)
                        gTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCCCGGAtt                                                                          >  2:1313819/1‑100 (MQ=255)
                          gACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTa                                                                        >  1:339034/1‑100 (MQ=255)
                            cATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTAtt                                                                      <  1:1934488/100‑1 (MQ=255)
                                   ccGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcg                                                               <  2:3413195/100‑1 (MQ=255)
                                    cGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgc                                                              <  1:2873154/100‑1 (MQ=255)
                                     ggTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTcgcg                                                             >  1:2681297/1‑100 (MQ=255)
                                      gTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGt                                                            >  1:2392919/1‑100 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                          >  1:2943622/1‑100 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTCGAAGTTTATCCGCGGATTTATTGATGTCGCGTc                                                           >  2:442595/1‑99 (MQ=255)
                                        cAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTAGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTcc                                                          >  2:1030364/1‑100 (MQ=255)
                                           cGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGc                                                       >  1:838652/1‑100 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                      <  2:137763/100‑1 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                      <  1:3089580/100‑1 (MQ=255)
                                            gAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCg                                                      <  1:639608/100‑1 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTg                                                    <  1:1571103/100‑1 (MQ=255)
                                              aaTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGGCGCGTCCAGCGTg                                                    <  2:1449809/100‑1 (MQ=255)
                                               aTCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGc                                                   <  2:304881/100‑1 (MQ=255)
                                                 cGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGccc                                                 <  1:220019/100‑1 (MQ=255)
                                                   gATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCgg                                               >  1:689486/1‑100 (MQ=255)
                                                   gATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCgg                                               <  1:3323040/100‑1 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCg                                                <  2:247601/98‑1 (MQ=255)
                                                    aTAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGc                                              <  1:3646124/100‑1 (MQ=255)
                                                       gTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGt                                           <  1:1678400/100‑1 (MQ=255)
                                                         tAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGa                                         >  1:1349218/1‑100 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                        >  2:1781570/1‑100 (MQ=255)
                                                          aaaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaa                                        >  1:629774/1‑100 (MQ=255)
                                                           aaCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGaaa                                       <  2:3055485/100‑1 (MQ=255)
                                                            aCGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                      <  1:2160341/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                     <  1:413324/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAcc                                     <  2:1202401/100‑1 (MQ=255)
                                                             cGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAc                                      <  2:206703/99‑1 (MQ=255)
                                                               ccTTCAGAATTGTACCAAGCTCAACGTTTTCCATCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                   >  2:2902927/1‑100 (MQ=255)
                                                               ccTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                   >  2:3582009/1‑100 (MQ=255)
                                                               ccTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTg                                   >  2:386877/1‑100 (MQ=255)
                                                                cTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGt                                  <  1:1971956/100‑1 (MQ=255)
                                                                 ttCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTc                                 <  2:2672602/100‑1 (MQ=255)
                                                                  tCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTcc                                <  2:1025987/100‑1 (MQ=255)
                                                                    aGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCAc                              <  2:833280/100‑1 (MQ=255)
                                                                       aTGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTCTCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGt                           <  1:2902950/100‑1 (MQ=255)
                                                                         ggTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTa                         <  2:322120/100‑1 (MQ=255)
                                                                          gTACCAATCTCAACGTTTTCCAGCCGTGGCTGGACGTTCATCCGCGGATTTATCGATGTCGCGTCCAGCCTGCCCGGCAGTGAAACCGGTCCCCCCTTa                         >  2:1283266/1‑99 (MQ=255)
                                                                          gTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTaa                        >  1:3383697/1‑100 (MQ=255)
                                                                           tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                       >  1:893138/1‑100 (MQ=255)
                                                                           tACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAg                       >  2:1720206/1‑100 (MQ=255)
                                                                            aCCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGt                      <  1:958562/100‑1 (MQ=255)
                                                                             ccAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGtt                     >  2:2060633/1‑100 (MQ=255)
                                                                             ccAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGtt                     >  2:1161827/1‑100 (MQ=255)
                                                                                aTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGc                  <  2:2159030/100‑1 (MQ=255)
                                                                                aTCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGc                  <  2:879268/100‑1 (MQ=255)
                                                                                 tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTg                   <  2:3450989/98‑1 (MQ=255)
                                                                                 tctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcc                 >  1:2742512/1‑100 (MQ=255)
                                                                                  ctcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGTCAGTGAAACCTGTCCACCGTTAAGTTTGcc                 >  2:1275372/1‑99 (MQ=255)
                                                                                  ccgatctacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGccc                <  1:250040/91‑1 (MQ=255)
                                                                                   tcAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCt               >  2:2184082/1‑100 (MQ=255)
                                                                                     ataaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc             >  1:2318642/7‑100 (MQ=255)
                                                                                     aaCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc             <  2:2584933/100‑1 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGc             <  2:2430271/99‑1 (MQ=255)
                                                                                      aCGTTTTCCAGCCGTGGCTGGAAGTTTATCCGAGGATTTATTGACGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCa            >  2:3169273/1‑100 (MQ=255)
                                                                                       aaacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTCCAg           >  2:885950/5‑100 (MQ=255)
                                                                                        gTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGt          <  2:1569738/100‑1 (MQ=255)
                                                                                        aacttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAAACTGTCCACCGTTAAGTTTGCCCTGCAGt          >  2:486325/4‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGcccagatcgg         >  2:250040/3‑93 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt         >  2:1572794/3‑100 (MQ=255)
                                                                                         acttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGtt         >  1:1585579/3‑100 (MQ=255)
                                                                                          tttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        <  2:782381/100‑1 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTCTTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGGCCACCGTTAAGTTTGCCCTGCAGTTg        >  2:1095774/2‑100 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        <  2:2318626/99‑1 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        >  2:3472242/2‑100 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        >  2:3522160/2‑100 (MQ=255)
                                                                                          cttCCAGCCACGGCTGGAAGTTTATCCGCGGACTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        >  1:3454125/2‑100 (MQ=255)
                                                                                           ttCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTg        <  1:2834383/99‑1 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg      <  1:3527762/100‑1 (MQ=255)
                                                                                            tCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAg      <  1:2619656/100‑1 (MQ=255)
                                                                                             ccAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGt     >  1:2781994/1‑100 (MQ=255)
                                                                                                  cGTAGCTCGAAGTTTACCCGCGGATTTATTGATGTCACGTCCAGCGTGCACGACATTGACACCTCTCCACCACTGAGCTTGCCCTGCCGTagagccat  >  2:3228439/1‑94 (MQ=255)
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CAGCACCGGAGAAATCACCAGCCAGTGACATTTTTCCGGTCAACGAAATCGGATAGTTAAACGCCTTCAGAATGGTACCAATCTCAACGTTTTCCAGCCGTGGCTGGAAGTTTATCCGCGGATTTATTGATGTCGCGTCCAGCGTGCCCGGCAGTGAAACCTGTCCACCGTTAAGTTTGCCCTGCAGTTGAGTAAT  >  NC_000913/2140301‑2140496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: