Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,313 T→A 19.4% intergenic (+26/+77) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3130TA19.4% 296.2 / 65.5 141intergenic (+26/+77)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (50/62);  new base A (16/11);  total (67/74)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

Only 100 of 142 total aligned reads displayed.

CACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCA  >  NC_000913/2286214‑2286406
                                                                                                   |                                                                                              
   gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                              <  2:539/99‑1 (MQ=255)
   gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                              <  2:683532/99‑1 (MQ=255)
   gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACg                                                                                             >  1:2834504/1‑100 (MQ=255)
    tAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACgg                                                                                            <  1:2223218/100‑1 (MQ=255)
     aGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                           >  1:1136388/1‑100 (MQ=255)
       cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttg                                                                                         <  2:3227065/100‑1 (MQ=255)
       cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTGCAAATCCTCTCGTGCCGACCAACAATCCCAAGAAAAAACCAACCGTAAGggttg                                                                                         >  1:1166319/1‑100 (MQ=255)
        gcAGCCTGGTAGCGCACCGTCAGGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACgggt                                                                                          >  2:2113696/1‑96 (MQ=255)
        gcAGCCTGGTAGCGCACCGTCAAGGGGCGTCGGGGGTCGGAGGCTCAAACCCCCTCGTGCCGACAAAAACTCCCAAGAAAAAACCCACCCTTACggttgg                                                                                        >  2:2116738/1‑100 (MQ=255)
          tccgatctAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                     <  2:2295501/93‑1 (MQ=255)
            ccTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttt                                                                                   <  2:2814279/100‑1 (MQ=39)
            ccTGGTAGAGCACCGGCAAGGGGTGTCGGGGGTCGGAGGTGCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttt                                                                                    <  2:2838206/99‑1 (MQ=37)
             cTGGAAGCGCACCGTCATGGGCTGTCGGGGGACGGAGGTTCAAAACCTCTCGTACCGACCAAAAATCCCCAGAAAAAACCAACCCTTACGGTTGGtttt                                                                                   <  2:2026990/99‑1 (MQ=255)
              tGGTAGCGCACCGTCATGGGGTGTCGGGTGGCGGAGGTTAAAATCCTCTCGTGCCGACCAAACATCCCAAGAAAAAACCAACCGTAAGGGTTGGtttttt                                                                                 <  2:2413583/100‑1 (MQ=37)
              tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                 <  1:742780/100‑1 (MQ=255)
               ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                <  2:625645/100‑1 (MQ=255)
               ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                <  1:3001199/100‑1 (MQ=255)
               ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                >  2:1047579/1‑100 (MQ=255)
               ggTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                <  1:2286944/100‑1 (MQ=255)
                gTAGCGCACCGTCATGGGGTGTCGTGCGTCGGAGGTTCAAAGCCTCTCGTGCCGTCCAAAAATCCCAATAAAAACCCAACCCTTACGGTTGGttttttt                                                                                <  2:2133397/99‑1 (MQ=255)
                gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttct                                                                               <  1:2245738/100‑3 (MQ=38)
                gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                               <  2:595501/100‑1 (MQ=255)
                gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                               <  1:724300/100‑1 (MQ=255)
                gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTta                                                                               <  1:1933964/100‑1 (MQ=255)
                 tAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                              >  1:1947822/1‑100 (MQ=255)
                  aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggttagatcgga                                                                             >  1:466886/1‑92 (MQ=255)
                  aGCGAACCGTCATGGGGTGTTAGGGGTCGGAGGTGCAAATCCTCTCATGACGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttt                                                                                  <  2:2594403/95‑1 (MQ=25)
                   gcgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttcttgg                                                                            <  1:1588248/100‑6 (MQ=37)
                     gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCt                                                                          >  1:25912/1‑100 (MQ=255)
                     gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCt                                                                          <  2:1347839/100‑1 (MQ=255)
                     gcACAGTCATGGGGTCCCGGGAGTCGCAGACTCAAATCCCCTCGTTCCGACCAAAAATCCCAAGAAAACACCAACCGTAAGGGTTAGtttcttct                                                                               <  2:2358583/95‑3 (MQ=255)
                      cACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTg                                                                         >  1:2071462/1‑100 (MQ=255)
                          gTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                     >  1:1122109/1‑100 (MQ=255)
                          gTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                     >  1:1671570/1‑100 (MQ=255)
                          gTCATGGGGTGTCGGGAGTCGGATGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCa                                                                       <  2:822007/98‑1 (MQ=255)
                           tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatt                                                                    <  2:2532128/100‑1 (MQ=255)
                           tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatt                                                                    >  2:2681205/1‑100 (MQ=255)
                            cATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaatta                                                                   <  1:3499551/100‑1 (MQ=255)
                              tGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaat                                                                 >  1:3074380/1‑100 (MQ=255)
                              tGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGATGGATTTTTTATATCTGCaattaat                                                                 >  2:3414466/1‑100 (MQ=255)
                               ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                >  1:1314623/1‑100 (MQ=255)
                               ggggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                >  1:1504995/1‑100 (MQ=255)
                                gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                               >  1:493597/1‑100 (MQ=255)
                                gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTc                                                               <  2:1379056/100‑1 (MQ=255)
                                       gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                        <  2:1906108/100‑1 (MQ=255)
                                       gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                        <  1:1993652/100‑1 (MQ=255)
                                        ggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                       <  1:1964111/100‑1 (MQ=255)
                                         gggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAg                                                      >  1:520227/1‑100 (MQ=255)
                                              ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGt                                                 >  1:1309042/1‑100 (MQ=255)
                                              ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGt                                                 >  2:3340960/1‑100 (MQ=255)
                                              ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGt                                                 >  1:2618648/1‑100 (MQ=255)
                                              ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGt                                                 >  2:3100338/1‑100 (MQ=255)
                                                 ggTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGac                                              >  1:85385/1‑100 (MQ=255)
                                                    taaaaTCCTCTCGTGCCTACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGac                                              <  2:419631/95‑1 (MQ=255)
                                                     cAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAGCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACAt                                          >  2:1949293/1‑100 (MQ=255)
                                                      aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATc                                         >  2:3151236/1‑100 (MQ=255)
                                                       aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATca                                        <  2:2618630/100‑1 (MQ=255)
                                                           ctctCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc                                    >  1:1640456/1‑100 (MQ=255)
                                                            tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                   <  2:1122100/100‑1 (MQ=255)
                                                            tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGcc                                   <  1:2527778/100‑1 (MQ=255)
                                                              tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTg                                 <  2:85385/100‑1 (MQ=255)
                                                                gTGCCGACCAACAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGtt                               <  1:1699664/100‑1 (MQ=255)
                                                                gTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGtt                               >  1:2005328/1‑100 (MQ=255)
                                                                 tGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAg                                     <  2:942202/93‑1 (MQ=255)
                                                                   ccGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAt                            >  1:1872787/1‑100 (MQ=255)
                                                                       ccAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                        <  1:3100361/100‑1 (MQ=255)
                                                                       ccAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                        >  1:355921/1‑100 (MQ=255)
                                                                         aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTGTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAttt                          <  2:2724468/97‑1 (MQ=255)
                                                                         aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTg                      <  2:1504987/100‑1 (MQ=255)
                                                                          ataaTCCCAAGAAAAAAACAACCCTTACTGTTAGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATTACAGCCTGGTTAGTGTCTGt                     <  2:43757/98‑1 (MQ=255)
                                                                          aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGt                     <  1:65891/100‑1 (MQ=255)
                                                                             aTCCCAAGAAAAAACCAACCCTTACTGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTAt                  <  2:93675/100‑1 (MQ=255)
                                                                              tCCCAAGAAAAAACCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                 <  1:851738/100‑1 (MQ=38)
                                                                              tCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                 <  1:1374421/100‑1 (MQ=255)
                                                                              agatataaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATc                 >  1:759761/8‑100 (MQ=37)
                                                                               cccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa                <  1:3238/100‑1 (MQ=255)
                                                                                ccAAGAAACAACCAACCCTTACGGCTGGTTTTTTTATATCTGCAATTAATTCGATATACAGACCGTGACTCATCATAGCATGTTTATTTTCTGTTATCAg               >  2:1657767/1‑100 (MQ=255)
                                                                                ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg               >  1:118651/1‑100 (MQ=255)
                                                                                 cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa              >  2:1889431/1‑100 (MQ=255)
                                                                                 cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa              >  1:1394344/1‑100 (MQ=255)
                                                                                  ataaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGaa             >  2:2815370/4‑100 (MQ=38)
                                                                                   taaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAGACAGACCGTTACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc            >  2:301490/3‑100 (MQ=38)
                                                                                   taaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc            >  1:1890527/3‑100 (MQ=38)
                                                                                    naaaaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTTTTTTCTGTTATCAGAACg           >  1:3265918/2‑100 (MQ=255)
                                                                                    gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg           >  1:3108356/1‑100 (MQ=255)
                                                                                    gAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg           <  2:1169231/100‑1 (MQ=255)
                                                                                    aaaaaaaCCAACCGTCAGGGGTGGTTTTTTTATATCTGCAATCAATACGATAAACAGACCGTGACCCCTCCCAGCCTGTTTATTTTCTGTAAt                  >  2:740945/2‑93 (MQ=25)
                                                                                    aaaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACg           >  1:320910/2‑100 (MQ=38)
                                                                                     aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc            >  2:1457232/1‑98 (MQ=255)
                                                                                      aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc         >  1:2059456/1‑100 (MQ=39)
                                                                                       aaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc        >  1:857570/1‑100 (MQ=39)
                                                                                       aaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc        >  1:2700724/1‑100 (MQ=255)
                                                                                         aaccaaccGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAg      >  1:2728061/1‑100 (MQ=255)
                                                                                         aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAg      >  1:1443760/1‑100 (MQ=255)
                                                                                          tctaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGa     <  1:1199012/97‑1 (MQ=255)
                                                                                           ccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAc    >  2:1383336/1‑100 (MQ=255)
                                                                                             aaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGaaga  >  2:1199004/1‑97 (MQ=255)
                                                                                             aaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACca  <  1:2428936/100‑1 (MQ=255)
                                                                                             aaccCTTACGGTTGGTATTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAc    <  2:3467623/98‑1 (MQ=255)
                                                                                              accTTTACGGTTGGTTTTTTTTTATCTGCAATTAATTAGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACca  <  2:2280387/99‑1 (MQ=255)
                                                                                                   |                                                                                              
CACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCA  >  NC_000913/2286214‑2286406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: