Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,750,720 C→T 100% intergenic (‑12/‑111) grpE ← / → nadK heat shock protein/NAD kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,7200CT99.2% 416.2 / ‑4.6 123intergenic (‑12/‑111)grpE/nadKheat shock protein/NAD kinase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (62/60);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 123 total aligned reads displayed.

CAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACA  >  NC_000913/2750624‑2750816
                                                                                                |                                                                                                
cAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtt                                                                                               >  2:859094/1‑100 (MQ=255)
cAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtt                                                                                               >  2:743471/1‑100 (MQ=255)
 aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttt                                                                                              <  1:427626/100‑1 (MQ=255)
 aaCTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTGTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttt                                                                                              >  1:1075135/1‑100 (MQ=255)
     gCCTCACTCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCTTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTGCTCCGTGttttttt                                                                                          <  2:1666223/100‑1 (MQ=255)
     gCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttttttt                                                                                          >  2:801837/1‑100 (MQ=255)
     gCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttttttt                                                                                          >  2:1650686/1‑100 (MQ=255)
      ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCCGGCGTTTTCTGTTCTTTAATACTCATGAATTTCTCCGTGTTTTTTTc                                                                                         >  2:2775800/1‑100 (MQ=255)
      ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc                                                                                         <  1:551172/100‑1 (MQ=255)
      ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc                                                                                         <  1:3188104/100‑1 (MQ=255)
      ccTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc                                                                                         >  1:1951699/1‑100 (MQ=255)
       ctctATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg                                                                                        <  2:976818/100‑1 (MQ=255)
       cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTc                                                                                         <  2:2677258/99‑1 (MQ=255)
       cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg                                                                                        >  2:2870038/1‑100 (MQ=255)
       cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg                                                                                        <  1:2473783/100‑1 (MQ=255)
       cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg                                                                                        <  2:1439432/100‑1 (MQ=255)
       cTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCg                                                                                        <  1:1229226/100‑1 (MQ=255)
        tCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGTGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGc                                                                                       <  1:610162/100‑1 (MQ=255)
        tCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGc                                                                                       >  1:1035619/1‑100 (MQ=255)
           aTCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCAtt                                                                                    >  1:3310292/1‑100 (MQ=255)
            tctcTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTc                                                                                   <  1:2189190/100‑1 (MQ=255)
             ctctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGttttttttttttttt                                                                                  >  2:751503/1‑92 (MQ=255)
              tctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAt                                                                                 >  1:354337/1‑100 (MQ=255)
               ctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGtttttttttttttttt                                                                                 >  2:1430/1‑90 (MQ=255)
               ctTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAt                                                                                 >  2:1873580/1‑99 (MQ=255)
                 tCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCAtctc                                                                              <  1:3626520/100‑1 (MQ=255)
                    tGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCt                                                                           >  2:3262190/1‑100 (MQ=255)
                     gCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCCTTCATCTCGCTa                                                                          <  1:2396738/100‑1 (MQ=255)
                      cTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTaa                                                                         >  2:36452/1‑100 (MQ=255)
                       tGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAAc                                                                        >  2:737315/1‑100 (MQ=255)
                        gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTTCTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACt                                                                       >  2:943890/1‑100 (MQ=255)
                        gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACt                                                                       >  2:3240060/1‑100 (MQ=255)
                        gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACt                                                                       >  2:1463664/1‑100 (MQ=255)
                        gATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACt                                                                       >  1:2189476/1‑100 (MQ=255)
                          tCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTc                                                                     >  2:3347651/1‑100 (MQ=255)
                           ccATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCg                                                                    <  1:1538127/100‑1 (MQ=255)
                            cATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGc                                                                   <  2:430712/100‑1 (MQ=255)
                              tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCtt                                                                 >  2:599130/1‑100 (MQ=255)
                              tGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCtt                                                                 <  2:1571894/100‑1 (MQ=255)
                               gATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCtta                                                                >  1:755251/1‑100 (MQ=255)
                               gATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCtta                                                                <  2:1534118/100‑1 (MQ=255)
                                  aaTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCttatta                                                             <  2:1102618/100‑1 (MQ=255)
                                  aaTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTATTTACTCCTCCTGAATTTCACCCTGTTTTTTTCGCAATCCTTTCGATCACTTGGCttatt                                                              >  1:2121285/1‑99 (MQ=255)
                                   aTTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTtattat                                                            >  1:1380239/1‑100 (MQ=255)
                                      tcttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATggg                                                         <  2:3521209/100‑1 (MQ=255)
                                       cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATgggg                                                        >  1:1658474/1‑100 (MQ=255)
                                       cttcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATgggg                                                        <  1:191397/100‑1 (MQ=255)
                                         tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                      <  2:115940/100‑1 (MQ=255)
                                         tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                      >  2:1824739/1‑100 (MQ=255)
                                         tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGAt                                                      <  2:2840804/100‑1 (MQ=255)
                                         tcCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACCACTCATGAATTTCTCCGTGTATTTTTGGCATTCATCTCGCTACCTTCGCTCATTATgcgg                                                        >  1:1382887/1‑98 (MQ=255)
                                           cGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCa                                                    >  1:2587265/1‑100 (MQ=255)
                                           cGGGGCCTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCTTGAATTTCTCCGTGTTTTTTTCGCATTCAGCTCGCTAACTTCGCTTATTATGGGGATc                                                     <  1:2847456/99‑1 (MQ=255)
                                            ggggCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCTCATTCTTCTCGCTAACTTCGCTTATTATgg                                                          >  2:421263/1‑93 (MQ=255)
                                            ggggCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAg                                                   <  1:801843/100‑1 (MQ=255)
                                               gCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGttt                                                >  1:1581188/1‑100 (MQ=255)
                                               gCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGttt                                                >  1:71707/1‑100 (MQ=255)
                                                 ttGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCAGGAATATCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTc                                               <  1:1700230/99‑1 (MQ=255)
                                                  tGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAg                                             <  2:951915/100‑1 (MQ=255)
                                                   gCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAgg                                            <  1:570552/100‑1 (MQ=255)
                                                   gCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAgg                                            <  2:1005142/100‑1 (MQ=255)
                                                   gCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAgg                                            <  1:30724/100‑1 (MQ=255)
                                                   gCCCCTCAGGCGTTTTCTGTTCTTTACTACTCAGTGATTTCTCCGTGTTTTTTTCGTATTCATCTCGCTAACTCCGCTTATTATTGGGATGCGttttcgg                                            >  2:3348223/1‑96 (MQ=255)
                                                    ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCTGATTCATTTCGCTAACTTCGCTTATTATGGGTCTCAGTTTCAggg                                           >  2:3044143/1‑100 (MQ=255)
                                                    ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                           >  1:1549446/1‑100 (MQ=255)
                                                    ccccTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAggg                                           >  1:2658354/1‑100 (MQ=255)
                                                      ccTCAGGCGTTTTCTGTTCTTTACTACTCAGGAATTTCTCCGTGTTTTTTTTGCATTCATCAAACTCACCTCTCTTCTTATGGGCATCAGTTTCCGGCtt                                         >  2:2423725/1‑100 (MQ=255)
                                                          aGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaa                                     <  2:1849389/100‑1 (MQ=255)
                                                          aGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCaa                                     <  2:1429524/100‑1 (MQ=255)
                                                            gCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTGAGGGTTTCAAgg                                   <  1:2393285/100‑1 (MQ=255)
                                                            gCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAgg                                   <  1:2747551/100‑1 (MQ=255)
                                                             cGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg                                  <  1:827110/100‑1 (MQ=255)
                                                             cGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAggg                                  >  2:3150099/1‑100 (MQ=255)
                                                              gTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGa                                 <  2:1792352/100‑1 (MQ=255)
                                                              gTTTTCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGa                                 <  1:386161/100‑1 (MQ=255)
                                                              cttttCTGTTCTTTACTCCTCATGAATTTCACCGTGTCTTTTTCGCATTCAGCACCCTAACTTCACCTATTATGGGGATCAGTTTCAGGGTTTCAAggg                                  >  2:1170954/2‑99 (MQ=255)
                                                                tttCTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAg                               <  2:2095593/100‑1 (MQ=255)
                                                                   cTGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCTTTTTTCTCCCTAACTTCGCTTATCCTGGGGATCCGTTTCAGGTTTTccctgtcagc                              >  2:511924/1‑89 (MQ=255)
                                                                    tGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACt                           >  2:3083064/1‑100 (MQ=255)
                                                                    tGTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACt                           <  2:3141744/100‑1 (MQ=255)
                                                                     gTTCTTTACTACTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTc                          >  1:1754240/1‑100 (MQ=255)
                                                                         tttACTACTCATGAATTTCTCCGTGTTTTTTTTTTTTTTTTTTTGCTAACTTCTCTTATTATGTTTTTCTGTTTCAGGGTTTCGAGGGAAGCACTCACAt                      >  2:2123399/1‑100 (MQ=255)
                                                                           tactacTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTg                    <  1:3613351/100‑1 (MQ=255)
                                                                           tactacTCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCCTATTATGGGGATCAGTTTCATGGTTTCAAGGGAAGCACTCACATTg                    <  2:539526/100‑1 (MQ=255)
                                                                            actactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGTTTTCAAGGGAAGCACTCACAtt                     >  2:3179927/1‑98 (MQ=255)
                                                                            actactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGt                   <  1:3472513/100‑1 (MQ=255)
                                                                             ctactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtc                  <  2:2536043/100‑1 (MQ=255)
                                                                              tactCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtca                 <  1:3268059/100‑1 (MQ=255)
                                                                               actCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcat                >  2:2716209/1‑100 (MQ=255)
                                                                                ctCATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatc               <  2:1976778/100‑1 (MQ=255)
                                                                                ctCATGAATTTCTCCGTGTTTTTTTCGCATCTACCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatc               >  2:2387662/1‑100 (MQ=255)
                                                                                  cATGAATTTCTCCGTGTTTTTTTTTTTTTTTTCTCGCTAACTTCGCCTATTATTTTTTTTTGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcctca              >  2:3132442/1‑99 (MQ=255)
                                                                                  cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGtcatc               >  2:3156540/1‑98 (MQ=255)
                                                                                  cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCaa             <  1:2993058/100‑1 (MQ=255)
                                                                                  cATGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAAATTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCaa             >  1:1809327/1‑100 (MQ=255)
                                                                                   aTGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCaa             <  1:2419551/99‑1 (MQ=255)
                                                                                   aTGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAAt            >  2:2530538/1‑100 (MQ=255)
                                                                                    tGAATTTCTCCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATc           <  2:3139581/100‑1 (MQ=255)
                                                                                          tctcCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGca     >  1:2283111/1‑100 (MQ=255)
                                                                                           ctcCGTGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGcaa    >  2:1806585/1‑100 (MQ=255)
                                                                                                |                                                                                                
CAACTGCCTCAATCTCTTCGTGCTGATCCATGATAATTTCTTCCGGGGCTTGCCCCTCAGGCGTTTTCTGTTCTTTACTACTCATGAATTTCTCCGCGTTTTTTTCGCATTCATCTCGCTAACTTCGCTTATTATGGGGATCAGTTTCAGGGTTTCAAGGGAAGCACTCACATTGTCATCAATCTTCGCAACA  >  NC_000913/2750624‑2750816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: