Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,772,227 A→T 15.0% pseudogene (840/900 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,772,2270AT15.0% 401.2 / 56.3 153pseudogene (840/900 nt)yfjVpseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
Reads supporting (aligned to +/- strand):  ref base A (70/59);  new base T (12/11);  total (83/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-02

Only 100 of 159 total aligned reads displayed.

TTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAAT  >  NC_000913/2772129‑2772325
                                                                                                  |                                                                                                  
  gtgATAGATGGTAATGTTTCCCCTATCAGTCTCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGt                                                                                                  >  2:1238277/1‑99 (MQ=255)
      tAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGa                                                                                             >  1:157900/1‑100 (MQ=255)
       aGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGAc                                                                                            <  2:1465350/100‑1 (MQ=255)
          tGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACaa                                                                                          >  2:1879902/1‑99 (MQ=255)
          tGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                                                                         <  1:2020126/100‑1 (MQ=255)
          tGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                                                                         >  1:1501543/1‑100 (MQ=255)
           gggaaTGTTGCTCATATCAGTATCTAATTGCAAAGTGACAGGGAGCCGCGACGCCAGCACGGCCAGAATAACAAAAAGAGCCGGCCACGGCGTGACAATg                                                                                        >  2:3454487/4‑100 (MQ=16)
            gTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGGCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGc                                                                                       >  2:3386947/1‑100 (MQ=255)
             tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGActgtgcc                                                                                      <  1:2625383/100‑7 (MQ=25)
             tAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGcc                                                                                      >  1:1769366/1‑100 (MQ=255)
               aTGTTGCTCATATCAGTATCTAATTACAAGGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                    <  2:2193259/100‑1 (MQ=255)
               aTGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                    <  2:3437732/100‑1 (MQ=255)
               aTGTTGCTCATATCAGTATCTAATGACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                    <  2:258811/100‑1 (MQ=255)
                tGTTGCACATATCAGTATCTAATTATAAAGTGACAGAGAGCCGCCTCGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                    <  2:1853564/99‑1 (MQ=255)
                  ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                 <  1:3365709/100‑3 (MQ=25)
                  ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                 <  1:741321/100‑3 (MQ=25)
                  ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                 <  2:1579428/100‑3 (MQ=25)
                   tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                 <  2:1216324/99‑3 (MQ=21)
                   tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACg                                                                                >  2:1847327/1‑100 (MQ=255)
                    gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctg                                                                               <  1:1748013/100‑5 (MQ=25)
                    gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACgg                                                                               >  1:2788443/1‑100 (MQ=255)
                     cTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctgt                                                                              <  2:630422/100‑6 (MQ=37)
                      tCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGaa                                                                             >  2:158235/1‑100 (MQ=255)
                       cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAg                                                                            <  1:1103607/100‑1 (MQ=255)
                        atatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctgtttg                                                                           <  1:230707/100‑9 (MQ=25)
                        atatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctgtttg                                                                           <  1:292576/100‑9 (MQ=25)
                          ntCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                                                         >  1:1598030/2‑100 (MQ=255)
                          atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                                                         >  2:1526178/1‑100 (MQ=255)
                          atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGGCATGACAATGCCGGTACGGAAGTaa                                                                         <  1:1238285/100‑1 (MQ=255)
                             aGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAatat                                                                      >  2:615376/1‑100 (MQ=255)
                                aTCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                                                   <  1:652553/100‑1 (MQ=255)
                                  cTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCa                                                                 >  2:3125699/1‑100 (MQ=255)
                                   tAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCa                                                                 >  1:2730873/1‑99 (MQ=255)
                                    aaTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAg                                                               <  2:3304693/100‑1 (MQ=255)
                                       tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGa                                                            >  2:2848010/1‑100 (MQ=255)
                                        aCAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAt                                                           <  2:434404/100‑1 (MQ=255)
                                         cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt                                                          >  1:1071570/1‑100 (MQ=255)
                                          aaaGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAt                                                           >  2:2980088/1‑98 (MQ=255)
                                           aaGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGt                                                        >  1:1343037/1‑100 (MQ=255)
                                             gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                                      <  1:659451/100‑1 (MQ=255)
                                             gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                                      <  1:397484/100‑1 (MQ=255)
                                              tGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCAACGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCat                                                     <  2:453622/100‑1 (MQ=255)
                                                 cAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt                                                  >  1:1868963/1‑100 (MQ=255)
                                                 cAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACAGTCCCAGTCATGACAAGGCCGGTACGGAAATAATATCCCCAAGTGATTGTcctgtt                                                  >  2:2033174/1‑95 (MQ=255)
                                                 cAGAGAGCCGCCAAGCCAGCGCGGCCAGAGTAACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtt                                                  >  1:2457788/1‑100 (MQ=255)
                                                   gagagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                                                >  1:828958/1‑100 (MQ=255)
                                                   gagagCCGCCACGCCAGCGCGGCCAGAGTGACAAACACCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                                                >  2:1940622/1‑100 (MQ=255)
                                                    agagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                                               <  1:604012/100‑1 (MQ=255)
                                                    agagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttt                                               >  1:1974688/1‑100 (MQ=255)
                                                      agCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTc                                              <  2:708901/99‑1 (MQ=255)
                                                      agCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCt                                             <  1:3318562/100‑1 (MQ=255)
                                                       gccgccACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                                            >  1:3525310/1‑100 (MQ=255)
                                                          gccacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGc                                         >  2:2823472/1‑100 (MQ=255)
                                                          gccacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGc                                         <  1:638937/100‑1 (MQ=255)
                                                          gccacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGc                                         >  1:1136988/1‑100 (MQ=255)
                                                             acgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAg                                      <  2:2762742/100‑1 (MQ=255)
                                                                      cgGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAg                             <  1:2199822/100‑1 (MQ=255)
                                                                       ggCCAGAGTGACAAACAGCGCCGGCACAGTGATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTGTTCTGGGCAAGCACATGc                               <  2:420762/97‑1 (MQ=255)
                                                                       ggCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGcacagatcgg                            >  2:2505186/1‑94 (MQ=255)
                                                                       ggCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                            <  2:1137361/100‑1 (MQ=255)
                                                                        gCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGcc                           >  2:373195/1‑100 (MQ=255)
                                                                        gCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGc                            <  2:474308/99‑1 (MQ=255)
                                                                          cAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCcac                         <  2:2730855/100‑1 (MQ=255)
                                                                          cAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCcac                         >  1:1818216/1‑100 (MQ=255)
                                                                           agagTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCcaca                        <  1:1522472/100‑1 (MQ=255)
                                                                            gagTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCAcag                       >  2:2904389/1‑100 (MQ=255)
                                                                            gagTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCAcag                       >  1:1597542/1‑100 (MQ=255)
                                                                            gagTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCAcag                       <  2:2444712/100‑1 (MQ=255)
                                                                               tGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCAcagcag                    <  1:493447/100‑1 (MQ=255)
                                                                               tGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGGCATATTTTTCTGGGCAAGCACATGCAGCCAcagcag                    >  2:1312696/1‑100 (MQ=255)
                                                                                gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                   >  1:3139307/1‑100 (MQ=255)
                                                                                gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                   >  1:2495530/1‑100 (MQ=255)
                                                                                gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                   >  1:1003852/1‑100 (MQ=255)
                                                                                 aCAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCagag                  >  2:1191583/1‑100 (MQ=255)
                                                                                 aCAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCagag                  <  2:3132830/100‑1 (MQ=255)
                                                                                  cAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGt                 >  2:923417/1‑100 (MQ=255)
                                                                                    ttccgtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGtt                >  2:2393651/7‑99 (MQ=37)
                                                                                    ttccgtaCCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTg               <  1:1665635/94‑1 (MQ=255)
                                                                                    aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTg               <  1:1624694/100‑1 (MQ=255)
                                                                                    aaCAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTg               >  1:2522456/1‑100 (MQ=255)
                                                                                      ccgtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGcc             >  2:2616544/5‑100 (MQ=37)
                                                                                      ccgtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGcc             >  1:1151673/5‑100 (MQ=37)
                                                                                       aGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa            >  1:347236/1‑100 (MQ=255)
                                                                                        gtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg           >  1:2827099/3‑100 (MQ=38)
                                                                                        gtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg           >  1:1780508/3‑100 (MQ=38)
                                                                                        gtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg           >  1:2591189/3‑100 (MQ=38)
                                                                                        gtaCCGGCATTGTCATGACTGTACCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg           >  2:2115049/3‑100 (MQ=37)
                                                                                        gCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg           >  2:2379975/1‑100 (MQ=255)
                                                                                        gCACCGGCACAGTCATGACAATGCCGGAACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCan           <  1:2221185/100‑2 (MQ=255)
                                                                                          aCCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGAc         <  1:1021259/100‑1 (MQ=255)
                                                                                          aCCGGCACAGTCATGACAATGCCGGTACGGAAGTAATAGCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGAc         <  2:3525285/100‑1 (MQ=255)
                                                                                           ccGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTACCAGa          >  2:789661/1‑98 (MQ=37)
                                                                                             ggCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGc      >  1:1338473/1‑100 (MQ=255)
                                                                                              tctcaGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGcc     <  1:570469/97‑1 (MQ=255)
                                                                                              gCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGcc     >  1:205449/1‑100 (MQ=255)
                                                                                              gCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGcc     <  1:96211/100‑1 (MQ=255)
                                                                                              gCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGcc     <  2:175275/100‑1 (MQ=255)
                                                                                               cacaGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCa    >  1:2663216/1‑100 (MQ=255)
                                                                                               cacaGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCa    >  1:304431/1‑100 (MQ=255)
                                                                                                 caGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCaga  >  2:570467/1‑98 (MQ=255)
                                                                                                  |                                                                                                  
TTGTGATAGATGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAAT  >  NC_000913/2772129‑2772325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: