Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,980,744 A→T 18.2% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,980,7440AT18.2% 362.3 / 71.6 153intergenic (‑109/+20)ygeA/araEAsp/Glu_racemase family protein/arabinose transporter
Reads supporting (aligned to +/- strand):  ref base A (82/43);  new base T (19/9);  total (101/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.86e-01

Only 100 of 156 total aligned reads displayed.

CTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCC  >  NC_000913/2980645‑2980842
                                                                                                   |                                                                                                  
cggATGTGTGTTACCGCAACAAGGCTGAATAAGTAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACa                                                                                                    <  2:3423737/98‑1 (MQ=255)
cTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACa                                                                                                    >  1:2895796/1‑100 (MQ=255)
  gATGTGTGTTACCGCAACAAGGCTGAATAAGGATCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGc                                                                                                  <  2:925036/100‑1 (MQ=255)
  gATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGc                                                                                                  >  1:71735/1‑100 (MQ=255)
  gATGGGTGTTAACGCAACAAGGCTGAATAAGGAAAGAAAAAAAAGCCTCAATGGCCAAGTGCCCAATAAAAAAAAAAAAAAAAAGGGCCGGATGTACAGc                                                                                                  <  2:3501772/100‑1 (MQ=255)
   atgtatgtTACCGCAACAAGGCTGAATAAGGAACGAAACAAAAGACTCAACGGCCAAGTGCCCAATAAAAAAAAAAAAAAAAAGGGCCGGATGTACAGca                                                                                                 <  2:798474/100‑1 (MQ=255)
   aTGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGca                                                                                                 <  2:284550/100‑1 (MQ=255)
     gtgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                               >  1:1467101/1‑100 (MQ=255)
     gtgtgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGcaca                                                                                               >  2:3552138/1‑100 (MQ=255)
        tgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                            >  2:3507072/1‑100 (MQ=255)
        tgtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATcc                                                                                            >  1:265130/1‑100 (MQ=255)
         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCg                                                                                           >  1:1675169/1‑100 (MQ=255)
         gtTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCg                                                                                           >  1:2270524/1‑100 (MQ=255)
           tACCGCAACAGGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGc                                                                                         <  2:192745/100‑1 (MQ=255)
           tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGc                                                                                         <  1:2893887/100‑1 (MQ=255)
           tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGc                                                                                         <  1:3313188/100‑1 (MQ=255)
           tACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGc                                                                                         >  1:1288892/1‑100 (MQ=255)
            aacGCAACAAGGCTGAAAAAGAAACAACAAAAACACATCAAGGGGCAAGTGCCCAATCAAAAAAAAAAAAAAAAGGGCCGGATGTGCTGTACATCCGGc                                                                                         <  2:1893981/97‑1 (MQ=9)
            aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGcc                                                                                        >  1:1224264/1‑100 (MQ=255)
            aCCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTAGTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGcc                                                                                        <  2:1950463/100‑1 (MQ=38)
             ccGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTGCTGTACATCCGGccc                                                                                       <  1:42011/100‑1 (MQ=39)
             ccGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGccc                                                                                       >  1:552150/1‑100 (MQ=255)
               gCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGt                                                                                     >  1:1834445/1‑100 (MQ=255)
                caacaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTg                                                                                    >  2:3645368/1‑100 (MQ=255)
                caacaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTg                                                                                    <  1:3054535/100‑1 (MQ=255)
                 aacaaGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATATCAATAAAAAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGa                                                                                   <  2:2660402/100‑1 (MQ=255)
                     aGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGCAAAAAGGACCGGATGTACAGCACATCCGGCCCGTGAAATc                                                                               >  1:41396/1‑100 (MQ=255)
                       gCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                             <  1:47183/100‑1 (MQ=255)
                        cTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGa                                                                            >  2:1686430/1‑100 (MQ=255)
                          gAAAAAGGAACGAGAAAAACGAAAAAACGGCAAAGTGAAAAAACTATATTAACAAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAg                                                                             <  2:106105/97‑1 (MQ=255)
                            tctaGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGcc                                                                        <  2:693787/97‑1 (MQ=255)
                               aGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGccaga                                                                     >  1:693790/1‑97 (MQ=255)
                               aGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGat                                                                     >  1:954798/1‑100 (MQ=255)
                                ggAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGata                                                                    >  1:1594806/1‑100 (MQ=255)
                                 gAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGatat                                                                   >  1:2240578/1‑100 (MQ=255)
                                  aaCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAtt                                                                  >  1:2403831/1‑100 (MQ=255)
                                  aaCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAtt                                                                  >  1:1853291/1‑100 (MQ=255)
                                   aCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                 >  1:3126807/1‑100 (MQ=255)
                                   aCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                 >  1:3190671/1‑100 (MQ=255)
                                   aCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                 <  1:2424559/100‑1 (MQ=255)
                                   aCGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATAttt                                                                 >  1:3548056/1‑100 (MQ=255)
                                      agaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTtctc                                                              >  2:1070103/1‑100 (MQ=255)
                                       gaCAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCa                                                             <  1:1485679/100‑1 (MQ=255)
                                         cAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAAc                                                           >  1:3645411/1‑100 (MQ=255)
                                               ccTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCg                                                     >  1:2571022/1‑100 (MQ=255)
                                                 tCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCCCGcc                                                   <  1:3566787/100‑1 (MQ=255)
                                                  cAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCt                                                  >  2:2156321/1‑100 (MQ=255)
                                                     cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                               >  2:2972961/1‑100 (MQ=255)
                                                     cGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGcc                                               >  2:2736875/1‑100 (MQ=255)
                                                      ggCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                              <  2:3190647/100‑1 (MQ=255)
                                                       gCCAAGTGCCTTATCCCTATTAGCAAAAAAATGGCCGGATGTATAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCa                                              <  2:2129647/99‑1 (MQ=255)
                                                         cAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCa                                           >  1:3440502/1‑100 (MQ=255)
                                                              gCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                      >  2:2826029/1‑100 (MQ=255)
                                                              gCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTg                                      >  2:849703/1‑100 (MQ=255)
                                                               cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAACTCAGACGCCGATATTCCTCAACTTCTCGCCTGCCATCAGTTTGc                                     >  2:766029/1‑100 (MQ=255)
                                                               cccAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGc                                     >  1:991987/1‑100 (MQ=255)
                                                                 cAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGt                                   <  1:85424/100‑1 (MQ=255)
                                                                  aaTCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGtt                                  >  2:2716685/1‑100 (MQ=255)
                                                                       ctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCCACTTCTCGCCTGCCATCAGTTTGCGTTCGata                             >  2:889529/1‑100 (MQ=255)
                                                                       ctATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGata                             >  2:2361941/1‑100 (MQ=255)
                                                                           tAACGAAAAAAGCGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGtt                         <  2:41396/100‑1 (MQ=255)
                                                                            ctgatttcacgggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTc                        >  1:559739/11‑100 (MQ=25)
                                                                            aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTc                        >  2:1523326/1‑100 (MQ=255)
                                                                            aaCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTc                        >  2:612778/1‑100 (MQ=255)
                                                                             aacgaaaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                       >  1:2839440/5‑100 (MQ=255)
                                                                             aCGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCATCTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTcc                       <  2:1946524/100‑1 (MQ=255)
                                                                              cGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCa                      >  2:3361838/1‑100 (MQ=255)
                                                                              cGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCa                      >  1:986953/1‑100 (MQ=255)
                                                                              cGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCa                      >  1:161166/1‑100 (MQ=255)
                                                                              cGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCa                      >  1:1500643/1‑100 (MQ=255)
                                                                               gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                     <  1:2159953/100‑1 (MQ=255)
                                                                               gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                     <  2:3236331/100‑1 (MQ=255)
                                                                               gAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAg                     >  1:1449170/1‑100 (MQ=255)
                                                                                 naaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCg                   >  2:1883813/2‑100 (MQ=255)
                                                                                  aaaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGt                  <  2:2956813/100‑1 (MQ=255)
                                                                                   cacgggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTg                 >  1:378412/4‑100 (MQ=38)
                                                                                   aaaGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTg                 <  2:167396/100‑1 (MQ=255)
                                                                                      gggCCGGTTGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACa              >  1:1909466/1‑100 (MQ=255)
                                                                                      gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACa              >  1:3257368/1‑100 (MQ=39)
                                                                                      gggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTAGATATGTTCCAGCGTGCCa              >  1:346373/1‑100 (MQ=38)
                                                                                       ggCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAt             >  1:1193100/1‑100 (MQ=39)
                                                                                        gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt            >  2:1054898/1‑100 (MQ=255)
                                                                                        gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt            >  2:1500989/1‑100 (MQ=255)
                                                                                        gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt            >  2:3535205/1‑100 (MQ=255)
                                                                                        gCCGGATGTGCTGTACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAt             >  2:1305714/1‑99 (MQ=39)
                                                                                        gCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt            >  1:110544/1‑100 (MQ=255)
                                                                                        gCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtt            >  2:3463992/1‑100 (MQ=255)
                                                                                        gCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGATCCAGCGTGACAtt            >  2:1406969/1‑100 (MQ=255)
                                                                                          cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtttt          >  1:1668941/1‑100 (MQ=255)
                                                                                          cGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACAtttt          >  2:1818722/1‑100 (MQ=255)
                                                                                            gATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTg        <  1:1592211/100‑1 (MQ=255)
                                                                                             aTGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTgg       <  2:1853282/100‑1 (MQ=255)
                                                                                              tGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGt      >  2:2488955/1‑100 (MQ=255)
                                                                                                tACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGttt    >  1:847996/1‑100 (MQ=255)
                                                                                                 aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGttt    >  1:3602267/1‑99 (MQ=255)
                                                                                                 aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc   >  1:2056401/1‑100 (MQ=255)
                                                                                                 aCAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTc   >  1:315948/1‑100 (MQ=255)
                                                                                                  cAGCCCATCCCGCCCGTGAACTAAGACGCCGATATTTTTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGCTATGTTCCAGCGTGACATTTTTg        <  2:1206660/94‑1 (MQ=255)
                                                                                                  cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc  <  2:647809/100‑1 (MQ=255)
                                                                                                  cAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTcc  <  2:1310123/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CTGATGTGTGTTACCGCAACAAGGCTGAATAAGGAACGAGACAAACGCCTCAACGGCCAAGTGCCCAATCTCTATTAACGAAAAAAGGGCCGGATGTACAGCACATCCGGCCCGTGAAATCAGACGCCGATATTTCTCAACTTCTCGCCTGCCATCAGTTTGCGTTCGATATGTTCCAGCGTGACATTTTTGGTTTCC  >  NC_000913/2980645‑2980842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: