BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,182,803	T→G	15.5%	L85W (TTG→TGG)	zupT →	zinc transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,182,803	0	T	G	15.5%	326.5 / 58.0	136	L85W (TTG→TGG)	zupT	zinc transporter
Reads supporting (aligned to +/- strand): ref base T (57/58); new base G (13/8); total (70/66)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

Only 100 of 137 total aligned reads displayed.

GCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCT  >  NC_000913/3182705‑3182902

gCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCCGCTTGGCTATTTTGGCCCGGACCGCATGTTg                                                                                                    <  1:2457537/100‑1 (MQ=255)

   tCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca                                                                                                 >  1:1066122/1‑100 (MQ=255)

   tCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca                                                                                                 >  1:934417/1‑100 (MQ=255)

   tCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCca                                                                                                 <  1:272071/100‑1 (MQ=255)

     cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                               >  1:858896/1‑100 (MQ=255)

     cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                               >  2:2997709/1‑100 (MQ=255)

     cTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCcaca                                                                                               <  1:2188366/100‑1 (MQ=255)

           cACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                         <  1:1285750/100‑4 (MQ=255)

           cACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                         <  1:237341/100‑4 (MQ=255)

           cACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggt                                                                                         <  2:381457/100‑4 (MQ=255)

              tAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtcc                                                                                       <  2:1185782/99‑6 (MQ=255)

              tAGCAGCTGAAGGAATGTCGCATGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCGTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGGTCATc                                                                                       <  2:3199781/99‑1 (MQ=255)

               agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCg                                                                                     >  1:1341242/1‑100 (MQ=255)

               agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCg                                                                                     <  2:3502984/100‑1 (MQ=255)

               agcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTGGCAACATGcggtccag                                                                                     <  2:533913/100‑8 (MQ=255)

                gcagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATGTTGGCCTGGACCGCATGTGGCAACATGcggtccagg                                                                                    <  2:1660185/100‑9 (MQ=255)

                 tagcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCa                                                                                   <  2:287109/99‑1 (MQ=255)

                 cagcTGAAGGAATGTCGCCTGTATTGGGTTACGGAATCTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCa                                                                                   <  2:2461771/100‑1 (MQ=255)

                  agcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCaa                                                                                  >  1:287111/1‑99 (MQ=255)

                   gcTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAgg                                                                                 <  1:3058012/100‑1 (MQ=255)

                    cTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGa                                                                                >  1:724041/1‑100 (MQ=255)

                    cTGAAGGAATGTCGCCTGAGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGACATGGACTGCATGTTGCCACATTCTCATCCGc                                                                                    >  2:3101602/1‑96 (MQ=255)

                     tGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGAt                                                                               >  1:3123959/1‑100 (MQ=255)

                      gAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGAtt                                                                              >  1:3177944/1‑100 (MQ=255)

                       aaGGATTGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGTACCGCATGTTGCCACATGCTCATCCGCAGGAttt                                                                             <  2:3220620/100‑1 (MQ=255)

                          gAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAAt                                                                          <  1:2016634/100‑1 (MQ=255)

                           aaTGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATg                                                                         >  2:1710006/1‑100 (MQ=255)

                            aTGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGc                                                                        <  1:3109018/100‑1 (MQ=255)

                              gTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCaa                                                                      >  1:241408/1‑100 (MQ=255)

                                 gCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCaaaaa                                                                   >  1:214498/1‑100 (MQ=255)

                                  ccTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCCGGATTCAATGcca                                                                      >  2:3003438/1‑94 (MQ=255)

                                  ccTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAAt                                                                  >  1:1776317/1‑100 (MQ=255)

                                    tgtgTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCg                                                                <  1:205600/100‑1 (MQ=255)

                                     gtgtTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCgg                                                               >  2:1467531/1‑100 (MQ=255)

                                     gtgaggggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCCTCCGCAGGATTTAATGGAAAAAACg                                                                >  2:781963/6‑99 (MQ=255)

                                      tgtTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGt                                                              >  1:3515647/1‑100 (MQ=255)

                                          gggTTATGGAATTTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAg                                                          <  2:438714/100‑1 (MQ=255)

                                          gggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAg                                                          >  1:1053788/1‑100 (MQ=255)

                                           ggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGc                                                         >  1:218504/1‑100 (MQ=255)

                                           ggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGc                                                         >  1:119086/1‑100 (MQ=255)

                                           ggTTATGGAATGTTTATCTTCGGTCTGCTTGGCTAGTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCTGTGCAg                                                          <  2:1738634/99‑1 (MQ=255)

                                            gTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGAAGGATTTAATGCAAAAATCGGTGCAGcc                                                        >  1:1159315/1‑100 (MQ=255)

                                              tATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGt                                                      <  1:2965465/100‑1 (MQ=255)

                                              tATGGAACGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGt                                                      >  1:1917042/1‑100 (MQ=255)

                                                 ggAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                   >  2:1677150/1‑100 (MQ=255)

                                                 ggAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGc                                                   >  1:1462091/1‑100 (MQ=255)

                                                  gAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGTCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTTCAGACGTTGcc                                                  <  2:1917032/100‑1 (MQ=255)

                                                  gAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGcc                                                  >  2:1227560/1‑100 (MQ=255)

                                                  gAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGcc                                                  <  1:1406551/100‑1 (MQ=255)

                                                     tGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCaaa                                               <  1:2899996/100‑1 (MQ=255)

                                                     tGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCaaa                                               >  2:1465398/1‑100 (MQ=255)

                                                       tttATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAAt                                             >  1:2719037/1‑100 (MQ=255)

                                                         tATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCg                                           >  2:51387/1‑100 (MQ=255)

                                                            cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                        <  2:1679836/100‑1 (MQ=255)

                                                            cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                        >  2:2744826/1‑100 (MQ=255)

                                                            cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                        <  1:774590/100‑1 (MQ=255)

                                                            cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATCTAATGCAAAAATCGGTGCAGCCGTGGCCAAAATCGATc                                        <  2:2251466/100‑1 (MQ=255)

                                                            cTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGGTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                        >  2:1942361/1‑100 (MQ=255)

                                                            cTTCGGTCTGCTGGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTTACCCGCAGGTTTTAATGCCCACCTCGGTGCCGCCGGTCCCAAAATCGATc                                        >  2:638967/1‑100 (MQ=255)

                                                             ttCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATc                                        <  2:2690483/99‑1 (MQ=255)

                                                             ttCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCa                                       >  2:1406305/1‑100 (MQ=255)

                                                             ttCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCa                                       <  1:2300346/100‑1 (MQ=255)

                                                               cGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAg                                     <  1:1772402/100‑1 (MQ=255)

                                                               cGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAg                                     >  2:3595274/1‑100 (MQ=255)

                                                                ggTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAgc                                    >  1:2968595/1‑100 (MQ=255)

                                                                 gTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAgcg                                   <  1:470046/100‑1 (MQ=255)

                                                                 gTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAgcg                                   <  1:2335198/100‑1 (MQ=255)

                                                                    tGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGcac                                <  1:893589/100‑1 (MQ=255)

                                                                     gCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGcaca                               >  1:3406020/1‑100 (MQ=255)

                                                                       ttGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGc                             <  2:1899043/100‑1 (MQ=255)

                                                                         ggCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCa                           <  2:2100576/100‑1 (MQ=255)

                                                                           cTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCAtt                         >  2:2844093/1‑100 (MQ=255)

                                                                             aTTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCt                       <  1:2545975/100‑1 (MQ=255)

                                                                                 tGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTc                   <  2:1822070/100‑1 (MQ=255)

                                                                                  ggCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCa                  >  2:1323686/1‑100 (MQ=255)

                                                                                  ctgcggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCa                  >  1:2550550/11‑100 (MQ=38)

                                                                                   tgcggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                 >  1:911452/10‑100 (MQ=255)

                                                                                   tgcggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                 >  1:431538/10‑100 (MQ=255)

                                                                                   gCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                 <  2:1901928/100‑1 (MQ=255)

                                                                                   gCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                 <  2:1776309/100‑1 (MQ=255)

                                                                                   gCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAc                 <  1:2713005/100‑1 (MQ=255)

                                                                                    gcggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAct                >  2:3494203/9‑100 (MQ=255)

                                                                                     cggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCActc               >  1:2405215/8‑100 (MQ=255)

                                                                                     cTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCActc               >  2:2687918/1‑100 (MQ=255)

                                                                                     cTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCActc               >  1:3268689/1‑100 (MQ=255)

                                                                                      ggatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCActct              >  2:2768088/7‑100 (MQ=255)

                                                                                       gatgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACtctc             >  2:2220065/6‑100 (MQ=255)

                                                                                       gatcagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCAct                >  2:843201/6‑97 (MQ=255)

                                                                                        atgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCg            >  2:2732008/5‑100 (MQ=255)

                                                                                         tgagCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCgg           >  2:1595082/4‑100 (MQ=255)

                                                                                         aCCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCgg           <  2:2893904/100‑1 (MQ=255)

                                                                                         aCCGCATGTTGCCACATGCTCATCCACAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCgg           <  1:3096544/100‑1 (MQ=255)

                                                                                           cGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAACATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCa         <  2:1882463/100‑1 (MQ=255)

                                                                                           cGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCa         <  2:580293/100‑1 (MQ=255)

                                                                                           cGCATGTTGCCACATGCTCATCCGCAGAATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCAATCAAGCGCACAGCCATTCTGCTCACTCTCGGc          >  2:347278/1‑99 (MQ=255)

                                                                                            gCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCAt        >  1:1434140/1‑100 (MQ=255)

                                                                                            gCATGTGGCAACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCAt        >  1:3601786/1‑100 (MQ=255)

                                                                                               tGTTGCCACATGCTCATCCGCAGTATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAg     <  2:824373/100‑1 (MQ=255)

                                                                                               tGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAg     >  2:2009688/1‑100 (MQ=255)

                                                                                                  tGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCt  >  2:2677529/1‑100 (MQ=255)

GCTTCCTGCCGCACTAGCAGCTGAAGGAATGTCGCCTGTGTTGGGTTATGGAATGTTTATCTTCGGTCTGCTTGGCTATTTTGGCCTGGACCGCATGTTGCCACATGCTCATCCGCAGGATTTAATGCAAAAATCGGTGCAGCCGTTGCCAAAATCGATCAAGCGCACAGCCATTCTGCTCACTCTCGGCATCAGTCT  >  NC_000913/3182705‑3182902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`