Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 78,831 T→A 17.8% intergenic (+32/+17) setA → / ← leuD broad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091378,8310TA17.8% 358.5 / 66.6 154intergenic (+32/+17)setA/leuDbroad specificity sugar efflux system/3‑isopropylmalate dehydratase small subunit
Reads supporting (aligned to +/- strand):  ref base T (62/63);  new base A (6/21);  total (69/85)
Fisher's exact test for biased strand distribution p-value = 1.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

Only 100 of 161 total aligned reads displayed.

TCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGAC  >  NC_000913/78732‑78930
                                                                                                   |                                                                                                   
 cTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAttt                                                                                                    >  1:779943/1‑100 (MQ=255)
  tACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTg                                                                                                   <  1:2479398/100‑1 (MQ=255)
     tGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGAcc                                                                                                <  1:1038583/100‑1 (MQ=255)
      tgttAATTGCGGTTATTGCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCTGTCATTTGACCg                                                                                               <  2:1970399/97‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  2:675084/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  1:739464/100‑1 (MQ=255)
        gTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCggg                                                                                             <  1:771058/100‑1 (MQ=255)
         tAATTGCGTTTATTTCTGTTGTCGCATTATTTTTAACAGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  2:266554/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCggg                                                                                             <  2:3194049/99‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCggg                                                                                             <  1:2873452/99‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:3246529/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:141931/100‑1 (MQ=255)
         tAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGc                                                                                            <  1:2859040/100‑1 (MQ=255)
          aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATCGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                           <  1:238757/100‑2 (MQ=255)
          aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                           <  1:1962078/100‑2 (MQ=255)
          aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                           <  2:2376142/100‑2 (MQ=255)
          aaTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                           <  1:3422795/100‑2 (MQ=255)
          aaTTGCGGTTATTTCCGTTGTCGCATTAGTGTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGct                                                                                           <  2:1721536/100‑2 (MQ=255)
           aTTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctt                                                                                          <  1:3149689/100‑3 (MQ=255)
             tGCGGTTATTTCTGTTGTGGCAGTATTTGTAACCGCAAAGGCAAAAGCCGTTTGATGACGTGGACGATAGCGGAAAGAGCGGTCAAATGACCGGGct                                                                                           <  2:1520262/97‑2 (MQ=255)
              gCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAggg                                                                                       >  1:3365492/1‑100 (MQ=255)
              gCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTCGACCGGGCAAggg                                                                                       <  2:2253407/100‑1 (MQ=255)
                gggtATTTCTGGTGTCGGGTTATTTTTTTCCGAAAAGGTTAAAGACGTTTCAGGACGATGGCGTTCGCGGAAAGGCCGTTCAAATGACCGGGctttccg                                                                                      <  2:1060143/96‑7 (MQ=255)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAgggg                                                                                      <  2:1218810/99‑1 (MQ=255)
                ggTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCAAATGACCGGGctttccgc                                                                                     <  1:3044816/100‑8 (MQ=38)
                 gTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGAt                                                                                    <  2:3157430/100‑1 (MQ=255)
                   tATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTa                                                                                  >  2:309420/1‑100 (MQ=255)
                     tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                >  1:3053676/1‑100 (MQ=255)
                     tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                <  2:3441066/100‑1 (MQ=255)
                     tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                >  2:3484206/1‑100 (MQ=255)
                     tttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAt                                                                                <  2:3090093/100‑1 (MQ=255)
                      ttCTGTTGTCGCATTATTTTTAACCGTAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAAtt                                                                               <  2:2785904/100‑1 (MQ=255)
                      ttCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGCTTAAtt                                                                               >  2:2354060/1‑100 (MQ=255)
                       tCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTc                                                                              <  2:2496033/100‑1 (MQ=255)
                       tCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTc                                                                              >  2:600922/1‑100 (MQ=255)
                         ttttgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACTTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCa                                                                             <  1:184733/97‑1 (MQ=255)
                          gttgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATa                                                                           <  1:2197951/100‑1 (MQ=255)
                          gttgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATa                                                                           >  1:652088/1‑100 (MQ=255)
                          gttgtCGCATTATTGTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGCCATCTGACCGGGCAAGGGGCTTAATACATa                                                                           <  2:1211284/100‑1 (MQ=255)
                            tgtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATaaa                                                                         >  1:968427/1‑100 (MQ=255)
                             gtCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAAc                                                                        >  1:2387064/1‑100 (MQ=255)
                                gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                     >  1:2696003/1‑100 (MQ=255)
                                gCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCa                                                                     <  2:2860212/100‑1 (MQ=255)
                                  attattTTTAACCGCAAAGGTTAAAGACGTTTGATGACTTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAgg                                                                   >  2:1448472/1‑100 (MQ=255)
                                  actattTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAg                                                                    >  1:2463145/3‑99 (MQ=255)
                                     attTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttg                                                                >  1:619628/1‑100 (MQ=255)
                                      tttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgt                                                               >  1:1172651/1‑100 (MQ=255)
                                       ttttAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGgttgtt                                                              <  2:1315377/100‑1 (MQ=255)
                                           aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                          <  1:1698372/100‑1 (MQ=255)
                                           aaCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGc                                                          >  2:2523116/1‑100 (MQ=255)
                                            aCCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCt                                                         <  2:1601270/100‑1 (MQ=255)
                                              cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATGGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                       <  2:3023790/100‑1 (MQ=255)
                                              cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                       >  1:3322112/1‑100 (MQ=255)
                                              cGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTc                                                       <  2:1596201/100‑1 (MQ=255)
                                               gCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCa                                                      <  2:3515052/100‑1 (MQ=255)
                                                   aGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAg                                                  >  1:3169494/1‑100 (MQ=255)
                                                    ggTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGc                                                 >  1:3608059/1‑100 (MQ=255)
                                                    ggTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGc                                                 >  2:2409479/1‑100 (MQ=255)
                                                    ggTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGc                                                 >  2:1869855/1‑100 (MQ=255)
                                                     gTTAAAGACGTTTGATGACGTGGAAGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAATCGCAGGTTGTTTTGCTTCATAAGCg                                                <  2:872120/100‑1 (MQ=255)
                                                       naaaGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGc                                              >  1:3499996/2‑100 (MQ=255)
                                                          aGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAAt                                           >  1:872624/1‑100 (MQ=255)
                                                            aCGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGCCCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATgg                                         <  1:1670769/100‑1 (MQ=255)
                                                              gTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGCTTAATGCATAAACGAAGGTTGTTTTGCTTCATAGGCGGCAATGGcg                                       >  2:1786807/1‑100 (MQ=255)
                                                              gTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcg                                       <  2:3611539/100‑1 (MQ=255)
                                                              gTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcg                                       >  2:452462/1‑100 (MQ=255)
                                                                 tgatgaCGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTAATAAACGAAGGTTGTTTTGCTTCATAAGCGGCAATGGcgacg                                    <  2:1074668/100‑1 (MQ=255)
                                                                   atgaCGTGGCCGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGcgtcgtc                                  >  1:1043077/1‑100 (MQ=255)
                                                                      aCGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtg                               >  2:338205/1‑100 (MQ=255)
                                                                        gTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgct                             >  2:1681855/1‑100 (MQ=255)
                                                                          ggACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGtgctgc                           >  1:3573855/1‑100 (MQ=255)
                                                                            aCGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCaa                         >  2:2377924/1‑100 (MQ=255)
                                                                             cGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAg                        <  2:181981/100‑1 (MQ=255)
                                                                              gATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCa                          <  2:1046571/97‑1 (MQ=255)
                                                                               aTAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGt                      >  1:2205880/1‑100 (MQ=255)
                                                                                 aGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTaa                    >  1:907281/1‑100 (MQ=255)
                                                                                  gCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAg                   >  2:1951077/1‑100 (MQ=255)
                                                                                  gCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAg                   >  1:957540/1‑100 (MQ=255)
                                                                                    gtaaaGCCCGGTCATTTGACCGGGCAAGGTGATTAATTCATAAACGCAGGTTGTTTTGGTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGc                  <  2:3132091/97‑1 (MQ=255)
                                                                                    ggAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGcc                 <  1:3331639/100‑1 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGCCCGGGCAAGGTGATTAATTCATAAACGTAGGTTATAGTGCTTCATACGCGGCAATGGCGTCGTCGTGCTGCAAGGTTAGcc                 <  1:199559/99‑1 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccn                <  2:2599928/100‑2 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                >  1:244199/1‑100 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                <  1:409567/100‑1 (MQ=255)
                                                                                     gAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGccc                <  1:2098398/100‑1 (MQ=255)
                                                                                      cttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCa               >  2:3316335/4‑100 (MQ=255)
                                                                                       ttgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCaa              >  1:3231031/3‑100 (MQ=255)
                                                                                        tgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt             >  1:513084/2‑100 (MQ=255)
                                                                                        tgCCCGGTCAAATGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt             >  1:1413259/2‑100 (MQ=255)
                                                                                        aGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAAt             <  1:1105261/100‑1 (MQ=255)
                                                                                         gCCCGGTCATTTGACCGGGCAAGGGGATTCATTCATAATCGTAGGTTGTTTTGGTTCTTAGGTGGCTATGCAGTCGTCGTCCTGGTAGGATAGCCCAAt             >  2:2160554/1‑99 (MQ=255)
                                                                                         gCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATa            >  1:3086245/1‑100 (MQ=255)
                                                                                          cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc           >  2:141231/1‑100 (MQ=255)
                                                                                          cccGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATAc           <  1:1814656/100‑1 (MQ=255)
                                                                                            tggTCAACTGACCGGGCAAGAGGATTAATTCATAAACGGAGGTTGTTTTGCTTCATATGCGGCAATGGCGTCGTGGGACTGCAAGGTAAGCCCAATAGTg         >  2:2933601/2‑100 (MQ=255)
                                                                                             ggTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGt        >  1:1482008/1‑100 (MQ=255)
                                                                                             ggTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGt        <  2:3531760/100‑1 (MQ=255)
                                                                                                 aTTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAg    >  2:81960/1‑100 (MQ=255)
                                                                                                  tttGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGa   >  1:1165922/1‑100 (MQ=255)
                                                                                                   ttGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGAc  <  2:698444/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAAAGCCCGGTCATTTGACCGGGCAAGGGGATTAATTCATAAACGCAGGTTGTTTTGCTTCATAAGCGGCAATGGCGTCGTCGTGCTGCAAGGTAAGCCCAATACTGTCCAGAC  >  NC_000913/78732‑78930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: