Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,918,299 A→G 15.7% intergenic (‑34/+17) atpG ← / ← atpA F1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,918,2990AG15.7% 394.0 / 64.1 172intergenic (‑34/+17)atpG/atpAF1 sector of membrane‑bound ATP synthase, gamma subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (79/66);  new base G (15/12);  total (94/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-01

Only 100 of 180 total aligned reads displayed.

TTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGT  >  NC_000913/3918200‑3918398
                                                                                                   |                                                                                                   
tttAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTaa                                                                                                     <  2:2572550/100‑1 (MQ=255)
 ttAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAg                                                                                                    <  1:267729/100‑1 (MQ=255)
   aGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                  <  1:759224/100‑1 (MQ=255)
   aGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTACTAAATGCCTTGCGGCCTGCCCTAAGGc                                                                                                  >  1:2387504/1‑100 (MQ=255)
    gTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCa                                                                                                 >  1:3442058/1‑100 (MQ=255)
    gTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCa                                                                                                 >  1:3442085/1‑100 (MQ=255)
    ccgATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCa                                                                                                 <  1:3106098/98‑1 (MQ=255)
     tGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCaa                                                                                                >  1:3378594/1‑100 (MQ=255)
      gATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAg                                                                                               >  1:941888/1‑100 (MQ=255)
      gATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAg                                                                                               >  2:1714699/1‑100 (MQ=255)
       aTCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTGTTTTGCGCCGTCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAg                                                                                               >  2:3329564/1‑99 (MQ=255)
        tCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgcc                                                                                             <  2:1621692/100‑1 (MQ=255)
         cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgcc                                                                                             >  2:2880216/1‑99 (MQ=255)
         cTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTGCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccg                                                                                            <  2:2379175/100‑1 (MQ=255)
          ttttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgc                                                                                           <  2:1560310/100‑1 (MQ=255)
           tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgcc                                                                                          <  1:2018365/100‑1 (MQ=255)
           tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgccgcc                                                                                          <  2:847249/100‑1 (MQ=255)
           tttGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAgatcgg                                                                                          >  2:3106075/1‑95 (MQ=255)
             tGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                        <  2:1869214/100‑1 (MQ=38)
              gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgcaacg                                                                                       <  1:2462999/100‑5 (MQ=38)
              gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGa                                                                                       >  2:3356897/1‑100 (MQ=255)
              gCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGa                                                                                       >  1:3601328/1‑100 (MQ=255)
              gCGTGTTCTGGACGCTGGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAgg                                                                                       <  1:893520/100‑2 (MQ=37)
                gtgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                     >  1:297530/1‑100 (MQ=255)
                gtgtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACg                                                                                     >  1:1152723/1‑100 (MQ=255)
                gtgtTCTGGACGCTTGCGATCTTACGACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGGCCGCAAg                                                                                        <  2:3400613/97‑1 (MQ=37)
                  gtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCTTGCCTTAGGGCAGgccgcaaggcatt                                                                                   <  1:3528505/100‑9 (MQ=25)
                  gtTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGtt                                                                                   >  1:459175/1‑100 (MQ=255)
                     cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                >  2:726065/1‑100 (MQ=255)
                     cTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                <  1:1258638/100‑1 (MQ=255)
                      tGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCa                                                                               >  1:1632221/1‑100 (MQ=255)
                      tGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCAGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTAcc                                                                                >  1:2500345/1‑99 (MQ=255)
                       ggACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCACGAGCTTCTCCTCAATGCCTTGCGGCCTGCACTAAGGCAACCCGCCCGCAGTTAcc                                                                                >  2:1468375/1‑98 (MQ=255)
                          cGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGAt                                                                           >  1:2597158/1‑100 (MQ=255)
                              tGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGTGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTggg                                                                       <  1:2469555/100‑1 (MQ=255)
                              ngCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTggg                                                                       >  1:636097/2‑100 (MQ=255)
                               gCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGGCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                      <  2:603162/100‑1 (MQ=255)
                               gCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGt                                                                      <  1:726068/100‑1 (MQ=255)
                                 gATCTGACTACGTATCTCTTTTGCGCCGGCCATGCGCTTCGCCTCAATGCCTTGCGGTCTGCCCTAAGGCAAGCCGCCAGACGTTACCGGGATTGGGt                                                                      <  2:2100431/98‑1 (MQ=255)
                                      tactacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTg                                                               <  1:2259260/100‑1 (MQ=255)
                                       actacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGa                                                              >  1:2772202/1‑100 (MQ=255)
                                         tacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                            <  2:2652558/100‑1 (MQ=255)
                                         tacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                            <  1:2658951/100‑1 (MQ=255)
                                         tacGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAg                                                            <  2:518264/100‑1 (MQ=255)
                                         tacGTATCGCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACTTTACCAGGATTGGGTTGCTTTGAAg                                                            <  2:1319853/100‑1 (MQ=255)
                                          acGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                           <  2:297654/100‑1 (MQ=255)
                                          acGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAgg                                                           >  2:2727258/1‑100 (MQ=255)
                                          acGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTG‑CCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                          >  2:2551136/1‑100 (MQ=255)
                                           cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                          >  2:2141278/1‑100 (MQ=255)
                                           cGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGa                                                          <  2:947357/100‑1 (MQ=255)
                                             tATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAAt                                                        >  2:1104036/1‑100 (MQ=255)
                                              aTCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTATCAGGATTGGGTTGCTTTGAAGGAATc                                                       <  1:2216420/100‑1 (MQ=255)
                                                  ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAgg                                                   >  1:769812/1‑100 (MQ=255)
                                                  ctTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAgg                                                   >  2:1234055/1‑100 (MQ=255)
                                                    tgtGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGAt                                                 <  2:460489/98‑1 (MQ=255)
                                                      tGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGc                                               >  2:2281109/1‑100 (MQ=255)
                                                       gcgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGcc                                              <  2:140901/100‑1 (MQ=255)
                                                        cgcCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCt                                             >  1:2435715/1‑100 (MQ=255)
                                                           cGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTc                                          <  1:1615365/100‑1 (MQ=255)
                                                            ggCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCa                                         <  1:1085593/100‑1 (MQ=255)
                                                              ccATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGCTTCGGTTGCTTTGACGGAATCGCGGATGCCCTTc                                          >  2:3554859/1‑97 (MQ=255)
                                                              ccATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGc                                       <  1:314612/100‑1 (MQ=255)
                                                               cATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCt                                      >  2:893653/1‑100 (MQ=255)
                                                               cATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCt                                      >  2:2938432/1‑100 (MQ=255)
                                                                 tGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTg                                    <  1:3091167/100‑1 (MQ=255)
                                                                   aGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGcc                                  >  1:427006/1‑100 (MQ=255)
                                                                         tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                            >  1:2719130/1‑100 (MQ=255)
                                                                         tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                            <  1:3139492/100‑1 (MQ=255)
                                                                         tcctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAt                            >  1:3518264/1‑100 (MQ=255)
                                                                          cctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAtt                           >  1:500084/1‑100 (MQ=255)
                                                                           ctcAATGCCTTGCGGCCTGGCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAttt                          <  2:1197540/100‑1 (MQ=255)
                                                                           ctcAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGAttt                          <  1:2540328/100‑1 (MQ=255)
                                                                             cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                        <  2:473984/100‑1 (MQ=255)
                                                                             cAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                        <  1:1106334/100‑1 (MQ=255)
                                                                              aaTGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTtcg                        >  2:1684623/1‑99 (MQ=255)
                                                                                  tctggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                   >  2:391593/5‑100 (MQ=38)
                                                                                  ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                   >  1:165264/1‑100 (MQ=255)
                                                                                  ccTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGtt                   >  2:3141898/1‑100 (MQ=255)
                                                                                   ctggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTg                  >  2:2116646/4‑100 (MQ=38)
                                                                                   ctggCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTg                  >  1:2953047/4‑100 (MQ=38)
                                                                                     agCGGCTTACCTTAGGTCAAGCCGCCAGACATTACCAGGAGTGGATTGCCTTGAAGGAATCGAGGATGCAGCTCACCTTGCGATCACTTTCCTAGTTGTa                >  2:3175076/2‑100 (MQ=255)
                                                                                      gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGGAGGAATCGGGGATGCCTTTCAGCGTGCCTTCGATTTCGTCGTTGTa                >  2:2445454/1‑99 (MQ=255)
                                                                                      gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg               >  1:2054800/1‑100 (MQ=255)
                                                                                      gCGGCTTGCCTTAGGGCAGGCCGCCAGACGTGACCAGGATTGGGTTGCTTTGAAGGAATCGAGGCTGCCTTTCAGCTTACATTCGAATtc                         >  2:3251296/1‑90 (MQ=25)
                                                                                      gCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg               <  2:2845594/100‑1 (MQ=255)
                                                                                      gCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAg               <  2:3018916/100‑1 (MQ=255)
                                                                                       cGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGGTTGGGTTGCTTTGAAGGAATGGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTtata                >  1:3626026/1‑98 (MQ=255)
                                                                                       cGGCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGc              >  1:156475/1‑100 (MQ=255)
                                                                                       cGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGc              >  2:788408/1‑100 (MQ=255)
                                                                                        ggCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGcc             >  2:1121311/1‑100 (MQ=255)
                                                                                        ggCTTGCCTTAGGGCAGGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGc              >  2:2417991/1‑99 (MQ=255)
                                                                                         gCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTACCTTCGATTTCGTCGTTGTAGCCa            >  2:1812421/1‑100 (MQ=255)
                                                                                            tGCCCTAAGGCAAGCCGCCAGACGTTACCAGGCTTGGGTTGCTTTGAAGGGATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCAcc          >  1:2950071/1‑99 (MQ=255)
                                                                                             gCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCgg        <  1:3220968/100‑1 (MQ=255)
                                                                                               ccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTc      >  2:1533041/1‑100 (MQ=255)
                                                                                               ccTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTc      >  1:2888943/1‑100 (MQ=255)
                                                                                                   aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt  <  2:3516307/100‑1 (MQ=255)
                                                                                                   aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt  <  1:2157904/100‑1 (MQ=255)
                                                                                                   aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt  >  2:753538/1‑100 (MQ=255)
                                                                                                   aGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGt  <  2:2024210/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TTTAGTGATCTTTTGCGTGTTCTGGACGCTTGCGATCTTACTACGTATCTCTTTTGCGCCGGCCATGAGCTTCTCCTCAATGCCTTGCGGCCTGCCCTAAGGCAAGCCGCCAGACGTTACCAGGATTGGGTTGCTTTGAAGGAATCGAGGATGCCTTTCAGCTTGCCTTCGATTTCGTCGTTGTAGCCACCGGTCTGGT  >  NC_000913/3918200‑3918398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑