Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 335,166 T→A 18.4% intergenic (‑144/‑114) yahC ← / → yahD putative inner membrane protein/ankyrin repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913335,1660TA18.4% 316.8 / 60.2 135intergenic (‑144/‑114)yahC/yahDputative inner membrane protein/ankyrin repeat protein
Reads supporting (aligned to +/- strand):  ref base T (51/59);  new base A (11/14);  total (62/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAA  >  NC_000913/335068‑335265
                                                                                                  |                                                                                                   
tgCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAAtatt                                                                                                    >  1:1917657/1‑100 (MQ=255)
 gCAGATTATAGAGAAAAAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTACACTACCGCACACTGCGAAAAGATTGTTGAAtatt                                                                                                    <  2:2012355/99‑1 (MQ=255)
 gCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAAtatta                                                                                                   <  2:66668/100‑1 (MQ=255)
 gCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGACAAAGAAACAAATATTGCAATACCGCACACTGCGAAAAGATAATTGAaaataa                                                                                                   >  2:2948126/1‑98 (MQ=255)
  cAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAAtattat                                                                                                  <  2:1674167/100‑1 (MQ=255)
  cAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAAtatta                                                                                                   >  2:1724318/1‑99 (MQ=255)
    gACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTc                                                                                                <  1:2749214/100‑1 (MQ=255)
     aCTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTca                                                                                               <  1:1687177/100‑1 (MQ=255)
         taGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTcaacaa                                                                                           >  1:1856681/1‑100 (MQ=255)
           gagaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACaaaa                                                                                         >  1:1800207/1‑100 (MQ=255)
           gagaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatc                                                                                         <  1:1961296/100‑3 (MQ=255)
           gagaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatc                                                                                         <  1:1962045/100‑3 (MQ=255)
           gagaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatc                                                                                         <  1:710101/100‑3 (MQ=255)
           gagaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatc                                                                                         <  1:3618265/100‑3 (MQ=255)
            aaaaaTAAAACCTGATTAAGTGAAAAGGTATCAGCTCAAATAAACAAATATTTCACTACCGCACCCTACGAAAAGATTGTTGAATAATATTcaacaatc                                                                                         <  2:2543893/97‑3 (MQ=255)
             gaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAg                                                                                       >  1:1705257/1‑100 (MQ=255)
             gaAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAg                                                                                       <  2:3409508/100‑1 (MQ=255)
              aaaTAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAgg                                                                                      <  1:2304150/100‑1 (MQ=255)
              aaaTAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatcttt                                                                                      <  1:3410197/100‑6 (MQ=255)
               aaTAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGc                                                                                     >  1:3144865/1‑100 (MQ=255)
               aaTAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatctttt                                                                                     <  2:1941202/100‑7 (MQ=255)
                aTAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCg                                                                                    >  1:2623082/1‑100 (MQ=255)
                 tAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCga                                                                                   <  1:1491860/100‑1 (MQ=255)
                 tAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatcttttcg                                                                                   <  1:1158830/100‑9 (MQ=38)
                 tAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATAATATTcaacaatcttttcg                                                                                   <  1:545302/100‑9 (MQ=38)
                  aaaaCCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCgag                                                                                  <  2:3154245/100‑1 (MQ=255)
                    aaCCTGATTGAGTGAAAGGGCAACGGATCAAAGCAACAAATATTGCACTACCGCACACTGCGCAAAGATTGTTGAATATTATTCAACAAAAACGcca                                                                                   >  2:3169709/1‑95 (MQ=255)
                     aCCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATa                                                                               >  2:1450345/1‑100 (MQ=255)
                     aCCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATa                                                                               <  1:309927/100‑1 (MQ=255)
                     aCCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATa                                                                               >  2:936952/1‑100 (MQ=255)
                     aCCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATa                                                                               >  1:2976053/1‑100 (MQ=255)
                        tGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATAATCCAACAAAAAGGCGAGATAAgg                                                                            >  2:2916127/1‑100 (MQ=255)
                         gATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGc                                                                           <  1:1261109/100‑1 (MQ=255)
                           ttGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGa                                                                         >  2:91924/1‑100 (MQ=255)
                           ttGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGa                                                                         >  1:3503444/1‑100 (MQ=255)
                            tGAGTGAAAGGGTAACGGGTAAAAGAAACAAATATTTCATTACCGCATACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCaaa                                                                        <  2:1250400/100‑1 (MQ=255)
                              aGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAAtt                                                                      <  1:949969/100‑1 (MQ=255)
                               gTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGAGAATTa                                                                     >  2:553396/1‑100 (MQ=255)
                               gTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGATGAATAATATTCAACAAAAAGGCGAGATACGGCGAATTa                                                                     >  2:2558105/1‑100 (MQ=255)
                                 gAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTaaa                                                                   >  1:2843555/1‑100 (MQ=255)
                                  aaaGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAAt                                                                  <  1:1245489/100‑1 (MQ=255)
                                     gggTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTa                                                               >  2:2790887/1‑100 (MQ=255)
                                     gggTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTa                                                               <  1:1547859/100‑1 (MQ=255)
                                          tCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGACTGTTGAATATTATTCGACAAAAAGGCGAGATACGGGGAATTAAATTTAAGCg                                                           >  2:3336902/1‑99 (MQ=255)
                                           cGGGTCAAAGAAACAAATATTGCACTACCGCGCACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATAAAAATTAAGCgg                                                          >  2:3582072/1‑99 (MQ=255)
                                           cGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAACAGGCGAGATACGGCGAATTAAATTTAAGCggg                                                         <  1:3451581/100‑1 (MQ=255)
                                           cGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCggg                                                         <  2:2083831/100‑1 (MQ=255)
                                           cGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCggg                                                         <  1:2083267/100‑1 (MQ=255)
                                            gggTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGt                                                        >  2:1946691/1‑100 (MQ=255)
                                            gggTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGt                                                        <  1:2075127/100‑1 (MQ=255)
                                             ggTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGtt                                                       >  2:581423/1‑100 (MQ=255)
                                             ggTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGt                                                        <  2:1389111/99‑1 (MQ=255)
                                                 aaaGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCttt                                                   >  2:283001/1‑100 (MQ=255)
                                                 aaaGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCttt                                                   >  2:2630033/1‑100 (MQ=255)
                                                  aaGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCtttt                                                  >  1:1587490/1‑100 (MQ=255)
                                                   aGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTa                                                 <  1:203326/100‑1 (MQ=255)
                                                    gAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTAt                                                >  1:3264783/1‑100 (MQ=255)
                                                     aaaCAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATc                                               <  1:2422847/100‑1 (MQ=255)
                                                     aaaCAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATc                                               <  1:2937335/100‑1 (MQ=255)
                                                      aaCAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCACCAAAAAGGCGAGATAAGGGGCATTAAATTCACGAGGGTTCttt                                                   >  2:2741364/1‑95 (MQ=255)
                                                      aaCAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCa                                              >  2:3627570/1‑100 (MQ=255)
                                                       aCAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAg                                             <  1:3045274/100‑1 (MQ=255)
                                                        cAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGa                                            >  1:2829941/1‑100 (MQ=255)
                                                        cAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGa                                            <  2:1793631/100‑1 (MQ=255)
                                                         aaaTATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTATTTTATCAGAt                                           >  1:1597644/1‑100 (MQ=255)
                                                           atatTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTa                                         >  1:3236763/1‑100 (MQ=255)
                                                            tatTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTAt                                        >  1:3624726/1‑100 (MQ=255)
                                                               tGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGGTTATCGt                                     <  2:1239261/100‑1 (MQ=255)
                                                               tGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGt                                     <  1:3172694/100‑1 (MQ=255)
                                                                gCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGtt                                    <  2:499010/100‑1 (MQ=255)
                                                                   cTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTg                                   <  2:1528420/98‑1 (MQ=255)
                                                                   cTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCt                                 >  2:1406051/1‑100 (MQ=255)
                                                                       cGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATtc                             <  1:1477397/100‑1 (MQ=255)
                                                                        gCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATtct                            <  2:357327/100‑1 (MQ=255)
                                                                           cacTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGTCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATtctc                           <  2:771239/98‑1 (MQ=255)
                                                                           cacTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCaa                         <  2:1557671/100‑1 (MQ=255)
                                                                               gCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGTGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCa                     <  2:132553/100‑1 (MQ=255)
                                                                                cGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATATGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCAt                    >  2:1883679/1‑100 (MQ=255)
                                                                                cGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCAt                    >  2:1611918/1‑100 (MQ=255)
                                                                                cGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTATTATCAGATTATCGTTGCTATTCTCAATTCAt                    <  2:2104381/100‑1 (MQ=255)
                                                                                 gAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATc                   <  1:1451572/100‑1 (MQ=255)
                                                                                  aaaaGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCg                  <  2:57558/100‑1 (MQ=255)
                                                                                   cctttttgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATcaa                 >  2:2203291/6‑98 (MQ=39)
                                                                                   cctttttgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGa                 >  2:1308097/6‑100 (MQ=255)
                                                                                   aaaGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGa                 <  2:1480959/100‑1 (MQ=255)
                                                                                    ctttttgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCGGATTATCGTTGCTATTCTCAATTCATCGaa                >  1:823342/5‑100 (MQ=255)
                                                                                       tttgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAAc             >  2:259520/2‑100 (MQ=255)
                                                                                       aTTGTTGAATATTATTCAACAAAAAGGCGAGCTACGGTGAATTAAATTTAAGCGGGTTCTTTTATCAGGTTATCGTTGCTATTCTCAATTCATCGAAAAc             <  2:2450931/100‑1 (MQ=255)
                                                                                         tgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAg           >  1:2144139/1‑100 (MQ=255)
                                                                                         tgttgAATAATATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAg           >  1:110486/1‑100 (MQ=255)
                                                                                         tgttgAATAATATACAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAg           >  1:2478016/1‑100 (MQ=255)
                                                                                         atctgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAg           <  2:1528070/97‑1 (MQ=255)
                                                                                          gttgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGc          >  1:759879/1‑100 (MQ=255)
                                                                                           ttgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGcc         <  1:1785090/100‑1 (MQ=255)
                                                                                           ttgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGcc         <  2:1917647/100‑1 (MQ=255)
                                                                                           ttgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGcc         <  1:2607675/100‑1 (MQ=255)
                                                                                             gAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAgagat       >  1:1528078/1‑96 (MQ=255)
                                                                                              aaTATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCcaca      <  1:555141/100‑1 (MQ=255)
                                                                                                 attattCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACaaaa   <  1:590240/100‑1 (MQ=255)
                                                                                                  ttattCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACaaaaa  >  1:116039/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGCAGACTATAGAGAAATAAAACCTGATTGAGTGAAAGGGTATCGGGTCAAAGAAACAAATATTGCACTACCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAA  >  NC_000913/335068‑335265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: