BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,265,670	T→G	15.0%	F453V (TTT→GTT)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,265,670	0	T	G	15.0%	350.9 / 49.3	137	F453V (TTT→GTT)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand): ref base T (57/59); new base G (13/8); total (70/67)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTA  >  NC_000913/4265571‑4265768

 gTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCtt                                                                                                   >  1:2137758/1‑100 (MQ=255)

 atgatgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCtt                                                                                                   >  2:503190/2‑100 (MQ=255)

  tgatgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCttt                                                                                                  <  2:316138/100‑1 (MQ=255)

   gatgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTa                                                                                                 <  2:179425/100‑1 (MQ=255)

     tgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAAc                                                                                               <  1:290894/100‑1 (MQ=255)

     tgaGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAAc                                                                                               <  1:909075/100‑1 (MQ=255)

       aGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACgg                                                                                             <  2:2842113/100‑1 (MQ=255)

        ggTGTATGACGAAAAGAGTAATTTAAGAGGCATCGCGAAAATGTTTATGGGTAAACAACGGAACGGCCGCAGCGGGGCGGTACGCCTGACCTTTAACg                                                                                              <  2:3442655/98‑1 (MQ=255)

        ggTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACgg                                                                                             >  2:3507264/1‑99 (MQ=255)

        ggTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGt                                                                                            >  1:500789/1‑100 (MQ=255)

          tgtATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCa                                                                                          >  2:2936962/1‑100 (MQ=255)

           gtATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCaa                                                                                         >  1:3223014/1‑100 (MQ=255)

           gtATCACAAAAACAGTGAGTTAACAGGCACCGCGAAAATCATCATCGGTAAACACCGTAACGGCCCAATCGGGACGGTAAGCCTGACCTTTAACGGTc                                                                                           <  2:3270934/98‑1 (MQ=255)

            tATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCGTTAAAGGTCagg                                                                                        <  1:1989926/100‑3 (MQ=255)

             aTCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATg                                                                                       >  2:809739/1‑100 (MQ=255)

             aTCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCGTTAAAGGTCaggc                                                                                       <  1:319324/100‑4 (MQ=255)

             aTCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCGTTAAAGGTCaggc                                                                                       <  1:1538697/100‑4 (MQ=255)

              tCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATgg                                                                                      <  2:1434259/100‑1 (MQ=255)

              tCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATgg                                                                                      <  1:1387342/100‑1 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCACTGGt                                                                                     >  1:1648193/1‑100 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGt                                                                                     >  2:2101933/1‑100 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGt                                                                                     >  2:2102713/1‑100 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGt                                                                                     >  2:2587446/1‑100 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGt                                                                                     <  1:401949/100‑1 (MQ=255)

               cACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCGTTAAAGGTCaggcg                                                                                      <  2:1833301/99‑5 (MQ=255)

                aCGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTc                                                                                    <  1:3340206/100‑1 (MQ=255)

                 cGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCTGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTcg                                                                                   <  1:3544825/100‑1 (MQ=255)

                 cGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTcg                                                                                   >  1:917208/1‑100 (MQ=255)

                   aaaaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTcgcg                                                                                 <  1:1046386/100‑1 (MQ=255)

                   aaaaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTcgcg                                                                                 <  2:2761375/100‑1 (MQ=255)

                     aaCAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCt                                                                               <  2:1740402/100‑1 (MQ=255)

                       cAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTc                                                                             <  2:571264/100‑1 (MQ=255)

                        aGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCg                                                                            <  2:89784/100‑1 (MQ=255)

                         gTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGa                                                                           >  2:2707377/1‑100 (MQ=255)

                         gTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGa                                                                           >  1:644806/1‑100 (MQ=255)

                          tGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCg                                                                            >  2:2494010/1‑98 (MQ=255)

                           gATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACa                                                                         <  1:277958/100‑1 (MQ=255)

                           gATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACa                                                                         <  1:1946167/100‑1 (MQ=255)

                           gATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACa                                                                         >  1:723734/1‑100 (MQ=255)

                             tttAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAAc                                                                       <  1:3229033/100‑1 (MQ=255)

                             tttAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAAc                                                                       <  1:3014758/100‑1 (MQ=255)

                             tttAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAAc                                                                       <  1:1397742/100‑1 (MQ=255)

                               tAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTa                                                                     >  1:2258842/1‑100 (MQ=255)

                                    ggCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCgg                                                                >  1:2285678/1‑100 (MQ=255)

                                    ggCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCgg                                                                <  2:396388/100‑1 (MQ=255)

                                     gCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCggg                                                               >  2:846903/1‑100 (MQ=255)

                                     gCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCggg                                                               <  1:1624357/100‑1 (MQ=255)

                                          gcgGAAATTATTATCGGTAAACAGCGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGc                                                          <  1:1571697/100‑1 (MQ=255)

                                           cgGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACTGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCa                                                         <  2:1951846/100‑1 (MQ=255)

                                            ggAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAg                                                        >  2:3206201/1‑100 (MQ=255)

                                             gAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGt                                                       <  2:733076/100‑1 (MQ=255)

                                              aaaTTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTa                                                      <  2:1302671/100‑1 (MQ=255)

                                                  tattatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgac                                                  <  2:1284049/100‑1 (MQ=255)

                                                   attatCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacg                                                 <  2:2965166/100‑1 (MQ=255)

                                                      atCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGCCAACTATGCGGGGCCGCAGTacgacgacg                                              >  2:3349437/1‑100 (MQ=255)

                                                      atCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgacg                                              >  1:380680/1‑100 (MQ=255)

                                                      atCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTacgacgacg                                              <  2:2745591/100‑1 (MQ=255)

                                                        cGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaa                                            >  1:1817048/1‑100 (MQ=255)

                                                          gTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaata                                          >  2:1128834/1‑100 (MQ=255)

                                                            aaaCAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataat                                        >  2:396575/1‑100 (MQ=255)

                                                            aaaCAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataat                                        <  2:1597924/100‑1 (MQ=255)

                                                            aaaCAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataat                                        >  1:2564534/1‑100 (MQ=255)

                                                              acaacGTAACGGCCCAATCGGGACGGTACTCCAGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGaataataa                                      <  2:2196814/100‑1 (MQ=255)

                                                                 acGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAatta                                   <  1:1032698/100‑1 (MQ=255)

                                                                  cGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAattat                                  >  2:3233904/1‑100 (MQ=255)

                                                                      aCGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTa                              <  2:812121/100‑1 (MQ=255)

                                                                      aCGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTa                              >  1:812127/1‑100 (MQ=255)

                                                                       cGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTAt                             <  1:1330453/100‑1 (MQ=255)

                                                                        ggCCCAAGCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATg                            <  1:3486192/100‑1 (MQ=255)

                                                                            cAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAAtt                        >  1:802322/1‑100 (MQ=255)

                                                                              aTCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAg                      >  1:2241315/1‑100 (MQ=255)

                                                                              aTCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAg                      <  1:2325562/100‑1 (MQ=255)

                                                                              aTCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAg                      <  1:53645/100‑1 (MQ=255)

                                                                               tCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAgg                     >  1:1242912/1‑100 (MQ=255)

                                                                                 gggACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTa                   <  2:267834/100‑1 (MQ=255)

                                                                                     cgcgaccattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTa               >  1:1418028/10‑100 (MQ=255)

                                                                                     cgcgaccattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTa               >  1:1765272/10‑100 (MQ=255)

                                                                                     cGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAAttn               <  2:2872369/100‑2 (MQ=255)

                                                                                     cGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTa               >  1:1137789/1‑100 (MQ=255)

                                                                                       gTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTaaa             >  1:1545658/1‑100 (MQ=255)

                                                                                       cgaccattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTaaa             >  1:2728047/8‑100 (MQ=255)

                                                                                        tACGCCTGATCTGTAGCGGCCAATGGTCGCGCTGCGACAACTATGCGGGTCCGCAGTGCGACGAAGAATAATAATTATTTTATGAATTAGGTAATTAAAg            <  2:3603067/100‑1 (MQ=255)

                                                                                        tACGCCTGACCTTGAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGAAGTACGACGACAAATAATAATTATTTTATGAATTAGGTAATTAAAg            <  2:2669814/100‑1 (MQ=255)

                                                                                        gaccattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAg            >  2:1554379/7‑100 (MQ=255)

                                                                                         accattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc           >  1:10355/6‑100 (MQ=255)

                                                                                         accattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc           >  2:3526373/6‑100 (MQ=255)

                                                                                         accattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGc           >  1:1160830/6‑100 (MQ=255)

                                                                                          cGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCa          >  2:3270469/1‑100 (MQ=255)

                                                                                          cGCCTGACCTTTAACGGTCAATGGTCGCGCATCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCa          <  2:754994/100‑1 (MQ=255)

                                                                                           gatctaCCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCa          <  2:1747076/94‑1 (MQ=255)

                                                                                           cattGACCGTTAAAGGTCACTGGCCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTATTTAACGCa          >  2:1608133/4‑99 (MQ=255)

                                                                                           cattGACCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaa         >  2:1588203/4‑100 (MQ=255)

                                                                                             ctaacttttAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAac       <  2:581797/94‑1 (MQ=255)

                                                                                               gACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAacac     >  2:1189160/1‑100 (MQ=255)

                                                                                                aCCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACt    <  1:3271668/100‑1 (MQ=255)

                                                                                                aCCGTTAAAGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCaagatcg    >  1:1747084/1‑95 (MQ=255)

                                                                                                  cTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACtt   >  1:610192/1‑99 (MQ=255)

                                                                                                  cTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTa  >  2:1120847/1‑100 (MQ=255)

                                                                                                  cTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTa  >  1:33817/1‑100 (MQ=255)

                                                                                                  cTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATCTTATGAATTAGGTAATTTAAGCAAACACtaa  <  1:2451434/100‑3 (MQ=255)

CGTGATGAGGTGTATCACGAAAACAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTAACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCAGTACGACGACGAATAATAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTA  >  NC_000913/4265571‑4265768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`