BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	843,749	C→A	100%	D578Y (GAT→TAT)	ybiO ←	mechanosensitive channel protein, intermediate conductance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	843,749	0	C	A	100.0%	466.8 / ‑6.7	140	D578Y (GAT→TAT)	ybiO	mechanosensitive channel protein, intermediate conductance
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (77/62); total (78/62)

Only 100 of 141 total aligned reads displayed.

GAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAATG  >  NC_000913/843653‑843844

gAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCCCCAAATATcc                                                                                              >  2:2266780/1‑100 (MQ=255)

gAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATcc                                                                                              <  1:3437346/100‑1 (MQ=255)

gAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATcc                                                                                              <  1:175811/100‑1 (MQ=255)

  atgatgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAg                                                                                            <  1:1269607/100‑1 (MQ=255)

     atgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAGAtcggaagagc                                                                                         >  2:1539054/1‑92 (MQ=255)

     atgTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAgtgt                                                                                         >  2:452540/1‑100 (MQ=255)

      tgtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGtt                                                                                        <  1:2146611/100‑1 (MQ=255)

       gtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGtt                                                                                        >  2:737892/1‑99 (MQ=255)

       gtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTc                                                                                       >  1:2686826/1‑100 (MQ=255)

       gtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTc                                                                                       >  1:1875375/1‑100 (MQ=255)

       gtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTc                                                                                       >  1:1840964/1‑100 (MQ=255)

       gtgATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTc                                                                                       >  2:396558/1‑100 (MQ=255)

         gATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCAt                                                                                     >  2:2540629/1‑100 (MQ=255)

         gATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCAt                                                                                     >  1:1595798/1‑100 (MQ=255)

          aTACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATg                                                                                    <  2:3100006/100‑1 (MQ=255)

           tACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGc                                                                                   <  1:3520969/100‑1 (MQ=255)

             cGCCCCGGTATCCTGTCTTACGCCCACAGAGCGAATCGACATCCGTCCCACTGTGCCGGTCAACGGCCCGATAATCACCAAATATCCAGGGTTCATGcc                                                                                  >  2:1271756/1‑99 (MQ=255)

             cGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCg                                                                                 >  1:3579308/1‑100 (MQ=255)

               ccccGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGtt                                                                               >  1:2038718/1‑100 (MQ=255)

                 ccGGTATCCTGTCGTACGCCCACGGAGCGACTCGACATCCGTTCCCCTGTGCCGGTCAACGGCCCGATCGCCACCAAATATCCAGTGTCCATGCCGctt                                                                              >  2:895215/1‑99 (MQ=255)

                 ccGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGtttt                                                                             <  1:1703879/100‑1 (MQ=255)

                    ttccgatctCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCaa                                                                          <  2:2422906/92‑1 (MQ=255)

                    gTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCa                                                                           <  2:1510225/99‑1 (MQ=255)

                     tATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCaaa                                                                         <  1:729490/100‑1 (MQ=255)

                     tATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCaaa                                                                         >  1:3572602/1‑100 (MQ=255)

                      aTCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAAc                                                                        <  2:2925081/100‑1 (MQ=255)

                      aTCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAAc                                                                        <  1:1140097/100‑1 (MQ=255)

                      aTCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAAc                                                                        <  1:135361/100‑1 (MQ=255)

                        ccTGTCGTACGCCGACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAACCGCCAAATATCCAGTGTTCATGCCGTTTTCAAACTg                                                                      >  2:2702353/1‑100 (MQ=255)

                        ccTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTg                                                                      <  2:1344697/100‑1 (MQ=255)

                        ccTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTg                                                                      >  2:415522/1‑100 (MQ=255)

                            tCGTACGCCCACGGAGCGAATAGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaata                                                                  <  1:875685/100‑1 (MQ=255)

                             cGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCaaagatcggaa                                                                 >  1:2422922/1‑92 (MQ=255)

                              gTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaataaa                                                                >  1:2169964/1‑100 (MQ=255)

                              gTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaataaa                                                                <  1:2550082/100‑1 (MQ=255)

                               tACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaataaat                                                               >  2:1692750/1‑100 (MQ=255)

                               tACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGaataaat                                                               >  2:309921/1‑100 (MQ=255)

                                 cGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAc                                                             >  2:1042997/1‑100 (MQ=255)

                                  gCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAcc                                                            >  2:82149/1‑100 (MQ=255)

                                   cccACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGGCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAccc                                                           >  1:266930/1‑100 (MQ=255)

                                    ccACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCTGATAGTCATCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATAcccc                                                          <  2:1128776/100‑1 (MQ=255)

                                      aCGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCgg                                                        <  1:1751808/100‑1 (MQ=255)

                                        ggAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCggcg                                                      <  1:3371655/100‑3 (MQ=255)

                                         gAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGa                                                     <  2:3620106/100‑1 (MQ=255)

                                          aGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGat                                                    >  1:358094/1‑100 (MQ=255)

                                           gCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTg                                                      <  2:1132097/97‑1 (MQ=255)

                                             gAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGataat                                                 <  1:1835976/100‑1 (MQ=255)

                                             gAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTCTTCAAACTGAATAAATACCCCGGTGataat                                                 >  2:3127133/1‑100 (MQ=255)

                                              aaTCGACATCCGGTCGACTGTGCCGGTCAACGGCCCGATAGTCACCCAAAATCCGGTGCTCATGCGGCCTTCAACCTGAATAAATAACCCGGAGagaata                                                >  2:1257571/1‑100 (MQ=255)

                                               aTCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAatat                                               >  2:1290448/1‑100 (MQ=255)

                                                  gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTGACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt                                            >  2:2248796/1‑100 (MQ=255)

                                                  gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt                                            >  1:1873310/1‑100 (MQ=255)

                                                  gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt                                            <  2:3572575/100‑1 (MQ=255)

                                                  gACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCtt                                            >  2:1053132/1‑100 (MQ=255)

                                                   aCATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCttt                                           <  1:2220764/100‑1 (MQ=255)

                                                      tCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGGTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCAc                                        >  1:2091063/1‑100 (MQ=255)

                                                       ccGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCAcc                                       >  2:3536476/1‑100 (MQ=255)

                                                       acGTTCCACTGTGCCGGTCCACGGGCCCATGGTCACTCACTCTCCCGCGTACCTGCCGCTTTCAACATCAATAAATACCCCGCTGATAATATCTTTAAcc                                       >  2:1651936/2‑100 (MQ=255)

                                                         gTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAg                                     >  1:2006797/1‑100 (MQ=255)

                                                           tCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCg                                   <  1:3166599/100‑1 (MQ=255)

                                                           tCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCg                                   <  1:269651/100‑1 (MQ=255)

                                                           tCCACTGTGCCGATCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCg                                   >  1:1988695/1‑100 (MQ=255)

                                                            ccACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGt                                  >  1:2393098/1‑100 (MQ=255)

                                                            ccACTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGCTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGc                                    >  2:2532120/1‑98 (MQ=255)

                                                              aCTGTGCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGttt                                >  1:2257850/1‑100 (MQ=255)

                                                                tgtgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGc                              <  1:3251186/100‑1 (MQ=255)

                                                                tgtgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGc                              <  2:1372526/100‑1 (MQ=255)

                                                                 gtgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCg                             >  1:1851795/1‑100 (MQ=255)

                                                                  tgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGa                            >  1:1742128/1‑100 (MQ=255)

                                                                  tgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGa                            >  1:2575333/1‑100 (MQ=255)

                                                                  tgCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGa                            <  2:1121948/100‑1 (MQ=255)

                                                                   gatcttCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaa                           <  2:1356042/95‑1 (MQ=255)

                                                                   gCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaa                           <  1:1227743/100‑1 (MQ=255)

                                                                   gCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaa                           >  1:2716170/1‑100 (MQ=255)

                                                                   gCCGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaa                           <  2:287901/100‑1 (MQ=255)

                                                                     cGGTCAACGGCCCGATAGTCACTAAATATCCAGTGTTTATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAc                          >  2:3271262/1‑99 (MQ=255)

                                                                     cGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAcc                         <  1:3172606/100‑1 (MQ=255)

                                                                     cGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAcc                         <  1:1574589/100‑1 (MQ=255)

                                                                     cGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAcc                         <  1:1570380/100‑1 (MQ=255)

                                                                     cGGTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAAcc                         <  2:2278436/100‑1 (MQ=255)

                                                                      ggTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCa                        >  2:2774447/1‑100 (MQ=255)

                                                                      ggTCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCa                        >  1:2871098/1‑100 (MQ=255)

                                                                      ggTCAACCGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCa                        >  2:1906395/1‑100 (MQ=255)

                                                                        tCAACGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGaaagatc                      >  1:1356050/1‑95 (MQ=255)

                                                                          aaCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACg                    <  2:55293/100‑1 (MQ=255)

                                                                           aCGGCCCGATAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACga                   >  1:364982/1‑100 (MQ=255)

                                                                                  gATAGTGACCAAATATCCAGTGCTCATGCCGTTTTCACACTAAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGcc            <  2:2460152/100‑1 (MQ=255)

                                                                                    tAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAg          >  1:2438306/1‑100 (MQ=255)

                                                                                    tAGTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAg          <  2:1775472/100‑1 (MQ=255)

                                                                                      gTCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGAc        >  1:2360277/1‑100 (MQ=255)

                                                                                       tCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGAcc       <  2:1875366/100‑1 (MQ=255)

                                                                                       tCACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTACGAACCAAACGAGATAGCCAGAcc       >  2:792480/1‑100 (MQ=255)

                                                                                        cACCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCt      >  1:1728607/1‑100 (MQ=255)

                                                                                        aaCCAAATATCCAGTGTTCATGACGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGACATAGCCAGACCt      >  2:1368493/2‑100 (MQ=255)

                                                                                         aCCAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGAcc       >  2:3455026/1‑98 (MQ=255)

                                                                                           cAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAAt   >  1:3037593/1‑100 (MQ=255)

                                                                                           cAAATATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAAt   >  1:823419/1‑100 (MQ=255)

                                                                                            aaaTATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAATg  >  1:907696/1‑100 (MQ=255)

                                                                                            aaaTATCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAATg  <  2:2615574/100‑1 (MQ=255)

                                                                                            aaaTATACAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATGTTTCACCAGCGATTGCGAACCAAACGAGATAGCCAGACCTaa    >  2:484916/1‑98 (MQ=255)

GAATGATGTGATACGCCCCGGTATCCTGTCGTACGCCCACGGAGCGAATCGACATCCGTTCCACTGTGCCGGTCAACGGCCCGATAGTCACCAAATCTCCAGTGTTCATGCCGTTTTCAAACTGAATAAATACCCCGGTGATAATATCTTTCACCAGCGTTTGCGAACCAAACGAGATAGCCAGACCTAATG  >  NC_000913/843653‑843844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`