BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,529	A→T	100%	T278S (ACC→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,529	0	A	T	99.0%	330.2 / ‑4.8	101	T278S (ACC→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (49/51); total (50/51)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 101 total aligned reads displayed.

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTG  >  NC_000913/1756433‑1756624

gACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTccc                                                                                              <  1:869386/100‑1 (MQ=255)

   cTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAACATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGa                                                                                           <  2:3276534/100‑1 (MQ=255)

    tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGAt                                                                                          >  1:1437451/1‑100 (MQ=255)

    tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGAt                                                                                          >  2:2067693/1‑100 (MQ=255)

    tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGAt                                                                                          >  2:412739/1‑100 (MQ=255)

     gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATg                                                                                         >  2:1522193/1‑100 (MQ=255)

     gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATg                                                                                         <  2:1961623/100‑1 (MQ=255)

     gggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATg                                                                                         >  1:2598039/1‑100 (MQ=255)

      ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGc                                                                                        <  1:604563/100‑1 (MQ=255)

       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                       >  1:3082418/1‑100 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                     <  1:1798276/100‑1 (MQ=255)

           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAt                                                                                   >  2:1023407/1‑100 (MQ=255)

           aGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGAt                                                                                   >  1:1899147/1‑100 (MQ=255)

            gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                  >  1:839753/1‑100 (MQ=255)

              aaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTcc                                                                                >  2:559094/1‑100 (MQ=255)

               aTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCa                                                                               <  2:1516425/100‑1 (MQ=255)

               aTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCa                                                                               <  2:1514691/100‑1 (MQ=255)

                tCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCAt                                                                              >  1:1398971/1‑100 (MQ=255)

                 cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCAt                                                                              >  2:69682/1‑99 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                            >  1:3094601/1‑100 (MQ=255)

                   cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCG‑AAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          <  2:1448077/100‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAt                                                                           <  2:113548/99‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          <  1:3585187/100‑1 (MQ=255)

                      tAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCTATGCCCGGAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaa                                                                        >  2:857538/1‑100 (MQ=255)

                       agaagaAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaa                                                                       >  1:2099637/1‑100 (MQ=255)

                        gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaaa                                                                      >  1:1875041/1‑100 (MQ=255)

                         aagaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaaaa                                                                     <  1:666596/100‑1 (MQ=255)

                          agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                    <  2:2601452/100‑1 (MQ=255)

                           gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAcc                                                                   <  1:2159916/100‑1 (MQ=255)

                           gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAcc                                                                   >  1:2060242/1‑100 (MQ=255)

                            aagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAccc                                                                  <  1:723487/100‑1 (MQ=255)

                             agTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCa                                                                 >  2:511423/1‑100 (MQ=255)

                                tATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGc                                                              <  1:734297/100‑1 (MQ=255)

                                 aTCTTCGCCCAGTAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCa                                                                 <  2:2491150/96‑1 (MQ=255)

                                 aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACTTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                             <  2:2335287/100‑1 (MQ=255)

                                 aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                             <  1:559096/100‑1 (MQ=255)

                                  tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                            >  1:1151647/1‑100 (MQ=255)

                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTTCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                            >  1:681949/1‑99 (MQ=255)

                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCAc                                                                >  2:2955621/1‑95 (MQ=255)

                                   cTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                                           <  2:1694439/100‑1 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          <  1:1807119/100‑1 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          >  1:1726174/1‑100 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          >  1:548996/1‑100 (MQ=255)

                                      cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                        <  1:3092966/100‑1 (MQ=255)

                                       gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTc                                                       <  1:1693192/100‑1 (MQ=255)

                                       gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTc                                                       >  2:1139451/1‑100 (MQ=255)

                                       gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTc                                                       >  1:2935726/1‑100 (MQ=255)

                                        cccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcg                                                      <  1:3323525/100‑1 (MQ=255)

                                         ccAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCGGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcg                                                      <  2:2019321/99‑1 (MQ=255)

                                                 tgatgatCGAAAAATGTATCCGTGCACGTAAAGTTGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACACGCTCAGAAGc                                             <  2:1912804/100‑1 (MQ=255)

                                                 tgatgatCGAAAAATGTATCCGTGCACGTAAAATCGTTATCACTGCGTCACAGATGCTGGATTTCATGATCAAAAACCCACGCCCGACTCGCGCAGaa                                               >  2:1725432/1‑98 (MQ=255)

                                                  gctgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                            <  2:2203279/98‑1 (MQ=255)

                                                  gatgatCTAAAAATGTATCCGTGCGCGTGAAGACGTTATCACTGCGTCCCAGATGCTGGATGGCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGcc                                            <  2:1402939/100‑1 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          >  2:839453/1‑100 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          >  2:1026081/1‑100 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          <  1:269139/100‑1 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          <  2:2727543/100‑1 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         >  1:220072/1‑100 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         >  1:2112926/1‑100 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         >  1:486481/1‑100 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         >  1:149336/1‑100 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                        >  2:1503677/1‑100 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                        <  1:3459328/100‑1 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCAACGCCCGACTCGCGCAGAAGCCGGTg                                        >  1:3080890/1‑100 (MQ=255)

                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                      >  1:217269/1‑100 (MQ=255)

                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                     >  2:3246786/1‑100 (MQ=255)

                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                     >  2:58378/1‑100 (MQ=255)

                                                         gAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg                                     >  1:301861/1‑100 (MQ=255)

                                                          aaaaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGt                                    <  2:3082395/100‑1 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                  <  1:1564491/100‑1 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                  <  2:2826104/100‑1 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                  <  1:3223348/100‑1 (MQ=255)

                                                            aaaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTg                                  <  1:2235378/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                 >  1:88322/1‑100 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                 <  1:2208545/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATACTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                 <  2:2325702/100‑1 (MQ=255)

                                                               tGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaa                               >  1:3233923/1‑100 (MQ=255)

                                                               tGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaa                               >  2:2532455/1‑100 (MQ=255)

                                                                  aTCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                            <  2:486479/100‑1 (MQ=255)

                                                                   tCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                           <  1:2423585/100‑1 (MQ=255)

                                                                       tGCACGCAGAGTCGTTACCCCTACATCCCAGATGCTGGATTCCACGATAAAACACCCATGGACCACGCCCGCTGAAGCCGGCGACCTTGcgatcttcatc                       >  2:1514325/1‑90 (MQ=255)

                                                                        gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                      >  1:1727353/1‑100 (MQ=255)

                                                                        gCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATcc                      >  2:2469874/1‑100 (MQ=255)

                                                                         cACTTAATGTCTTTGTCACTCCGTTCCAGATGCCGGATTCCATCATCAAAATCTCACGCCTGTCTCCCGCAGATGCCTGTGACGTTGCAAACGCAAGCCt                     <  2:2321069/100‑1 (MQ=255)

                                                                          aCGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                    >  2:2417760/1‑100 (MQ=255)

                                                                           cGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                   >  1:1340580/1‑100 (MQ=255)

                                                                            gTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCg                   >  2:2781125/1‑99 (MQ=255)

                                                                            gTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGa                  <  2:1151638/100‑1 (MQ=255)

                                                                             tAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                 <  1:10571/100‑1 (MQ=255)

                                                                              tcttccgatctCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACg                <  2:1513676/90‑1 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACggn              <  2:1340572/100‑2 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt              <  1:550998/100‑1 (MQ=255)

                                                                                 gTCGTTATCACTGTTTTCTAGATGCTGGGTTCCTTGATCAAAAACCCACGCCCGACTTGCGCTGAAGCCGGTGACGTTGCAAACGCCATCGTCGACg                <  2:86163/97‑1 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACggca             >  2:2211777/1‑98 (MQ=255)

                                                                                  tcttgaTCACTGCGTCCCAGATGCTGTAATCGATTGTCGATCATCCACGCCCGACCCGCGCAGAAGCCGGTGACGTTGCCCACTCCATCCTCGACGGTAc            <  2:885888/95‑1 (MQ=255)

                                                                                   cGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACt           >  1:2437373/1‑100 (MQ=255)

                                                                                        tCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc      <  1:2514870/100‑1 (MQ=255)

                                                                                        tCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGc      <  1:1846100/100‑1 (MQ=255)

                                                                                          aCTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg    >  1:1899470/1‑100 (MQ=255)

                                                                                            tGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg  <  1:1119964/100‑1 (MQ=255)

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTG  >  NC_000913/1756433‑1756624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`