Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,379,649	IS5 (–) +4 bp	coding (346‑349/1128 nt)	zapE ←	divisome ATPase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	87 (1.010)	62/198	0.3	98.8%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 3379652	1 (0.010)					coding (346/1128 nt)	zapE	divisome ATPase
*	?	NC_000913	= 2290113	NA (NA)	68 (0.790)	52/198	0.7	98.5%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	3379649 =	1 (0.010)					coding (349/1128 nt)	zapE	divisome ATPase

GTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2290019‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTT  >  NC_000913/3379649‑3379747                                                                                                                                                                                                    GTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGC                                                                                                >  1:300723/1‑100 GTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGC                                                                                                <  1:716524/100‑1   CGGCGAAGGTAGGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTC                                                                                              >  2:2283951/1‑100      CGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTC                                                                                           <  2:1448458/100‑1        AAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCAT                                                                                         <  2:25303/100‑1         AGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATG                                                                                        <  2:288002/100‑1          GGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGC                                                                                       >  2:688935/1‑100          GGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGC                                                                                       <  2:438361/100‑1          GGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGC                                                                                       <  1:167460/100‑1             gatcTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACC                                                                                    <  2:459721/96‑1               GTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCG                                                                                  <  2:745459/100‑1               GTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCG                                                                                  <  1:495968/100‑1                 tcttccgatcTGATGATCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCA                                                                                <  2:1344973/90‑1                  GATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCagatc                                                                               >  1:459719/1‑95                  GATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAG                                                                               <  1:688937/100‑1                   ATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGC                                                                              <  2:1673664/100‑1                       CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAA                                                                          >  2:1678786/1‑100                       CTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTAGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATA                                                                           >  1:1923671/1‑99                            GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGG                                                                     >  1:1511884/1‑100                             ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGT                                                                    >  2:1629024/1‑100                             ATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGT                                                                    >  1:397677/1‑100                              TGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCCTATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTG                                                                   >  2:1042006/1‑100                               GAAGCCTGCTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGCACTTCATGCACCCGCAGCATAAAACGGag                                                                   <  2:1388069/99‑3                               GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGA                                                                  >  2:83551/1‑100                                AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAA                                                                 >  1:1617490/1‑100                                 ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAA                                                                >  1:511299/1‑100                                 ACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAA                                                                >  2:480724/1‑100                                        TCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGG                                                         <  1:2159484/100‑1                                        TCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGG                                                         >  1:1907151/1‑100                                        TCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGG                                                         <  1:780877/100‑1                                         CTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGC                                                        <  2:1749652/100‑1                                             GGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTG                                                    >  1:1462763/1‑100                                               CTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTT                                                  >  1:810792/1‑100                                                 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTC                                                >  2:2111624/1‑100                                                 CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCCTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTC                                                <  1:2268882/100‑1                                                   AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCG                                              <  1:118285/100‑1                                                   AGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCG                                              >  2:1276012/1‑100                                                    GATGACAAACTTGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGTATTAAACGGTGAAAGTGCAGGCGCTGTTTCCGC                                             <  2:1511880/100‑1                                                    GATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGC                                             <  2:1872719/100‑1                                                     ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCT                                            <  2:899328/100‑1                                                         CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCC                                        <  1:2111632/100‑1                                                         CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCC                                        >  1:218028/1‑100                                                         CAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCACTGTTTCCGCTCTCC                                        >  2:1750285/1‑100                                                           AACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAGAGTGCAGGCGCTGTTTCCGCTCTCCCG                                      >  1:2123200/1‑100                                                            ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGG                                     >  2:1660189/1‑100                                                            ACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGG                                     <  2:2128404/100‑1                                                               TGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAG                                  <  2:249852/100‑1                                                                  TCTCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCT                               <  1:1660194/100‑1                                                                    TCATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTT                             >  2:984762/1‑100                                                                     CATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTG                            <  2:1907144/100‑1                                                                     CATATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTG                            >  1:354444/1‑100                                                                       TATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGAT                          <  1:772632/100‑1                                                                       TATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTG                            >  1:1090885/1‑98                                                                        ATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATA                         >  1:967862/1‑100                                                                        ATCAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATA                         <  1:1667008/100‑1                                                                          CAGGGACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGA                       <  2:397679/100‑1                                                                               ACTTGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAG                   <  2:218029/99‑1                                                                                  TGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCC               >  2:390541/1‑100                                                                                  TGTTCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCC               >  2:1854448/1‑100                                                                                     TCGCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATC            >  1:944862/1‑100                                                                                     TCGCACCATCCATAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATC            >  2:1216555/1‑100                                                                                       GCACCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAG          <  1:1315882/100‑1                                                                                          CCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCA       <  1:480722/100‑1                                                                                          CCTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCA       >  2:1190091/1‑100                                                                                           CTTCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAG      <  1:1276015/100‑1                                                                                             TCCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGT    <  1:1190094/100‑1                                                                                              CCTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCGCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGT    <  1:2006295/99‑1                                                                                               CTTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTT  >  1:1576948/1‑100                                                                                                                                                                                                    GTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2290019‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGCTCTTCATGCACCCGCAGCATAAAACGGTGAAAGTGCAGGCGCTGTTTCCGCTCTCCCGGCAGGCTTTGATAGAAAAGGTCCATCAGCCAGGTTT  >  NC_000913/3379649‑3379747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.