Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,759	G→A	intergenic (+80/‑11)	ygaH → / → mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,759	0	G	A	94.2%	265.6 / 5.3	86	intergenic (+80/‑11)	ygaH/mprA	putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base A (35/46);  total (37/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

TGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTC  >  NC_000913/2810663‑2810854                                                                                                 |                                                                                                tGATGGCGATTATATAACCCACAAGAATCCTTTTTCTAAAACAATACATTTACTTTATTTGTCACTTTCGTTACTATATCGGCTGAAATTAATGAGATCa                                                                                              <  2:1382231/100‑1 (MQ=255) tGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCa                                                                                              <  2:144826/100‑1 (MQ=255)  gATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                             <  1:1371967/100‑1 (MQ=255)   aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACGGTCGTTACTATATTGGCTGAAATTAATGAGGTCATa                                                                                            >  1:1621595/1‑100 (MQ=255)    tGGCGATTATAAAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAc                                                                                           >  2:1208470/1‑100 (MQ=255)     ggCGATTATATAACCCACAAGAATCATTTTTTTTTTTTTTTTCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCAt                                                                                             >  2:1705691/1‑97 (MQ=255)     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                          <  2:866454/100‑1 (MQ=255)     ggCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcc                                                                                          <  2:1300679/100‑1 (MQ=255)      cgatctatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAccc                                                                                         <  1:1375628/95‑1 (MQ=255)       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACCTTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCa                                                                                        <  2:765455/100‑1 (MQ=255)       cGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATAcccn                                                                                        <  2:1448085/100‑2 (MQ=255)       cGAGTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCa                                                                                        <  1:1086182/100‑1 (MQ=255)         aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCaaa                                                                                      >  1:833840/1‑100 (MQ=255)           tatataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATg                                                                                    <  2:2275100/100‑1 (MQ=255)            atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATgg                                                                                   >  2:131086/1‑100 (MQ=255)            atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATAGGCTGAAATTAATGAGATCATACCCagatcg                                                                                   >  2:1375625/1‑95 (MQ=255)            atataACCCACAAGAATCATTTTTCTAAAACAAAACATTTACTTTATTTTTCACCGTCGTTACTATATCGGAGGAACTTAATGAGATCATACCCAAATgg                                                                                   >  1:1312786/1‑100 (MQ=255)              ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATAAAGTCATACCCAAATGGAt                                                                                 <  1:1463951/100‑1 (MQ=255)                aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAg                                                                               <  1:1404436/100‑1 (MQ=255)                   ccACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTc                                                                            >  1:469294/1‑100 (MQ=255)                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                           <  1:441508/100‑1 (MQ=255)                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                           >  2:1795828/1‑100 (MQ=255)                    cacaAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCg                                                                           >  2:1989129/1‑100 (MQ=255)                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTGTATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                         <  1:846972/100‑1 (MQ=255)                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                         <  1:342897/100‑1 (MQ=255)                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                         <  2:833838/100‑1 (MQ=255)                      caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATACCGGCTGAAAGTAATGAGATCATACCCAAATGGATAGTTCGtt                                                                         >  2:1618739/1‑100 (MQ=255)                       aaGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGttt                                                                        <  2:117364/100‑1 (MQ=255)                        aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTa                                                                       <  1:1705696/100‑1 (MQ=255)                           aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGc                                                                    >  2:732513/1‑100 (MQ=255)                            tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGcc                                                                   >  2:23288/1‑100 (MQ=255)                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGccc                                                                  >  1:1031340/1‑100 (MQ=255)                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGccc                                                                  >  1:262602/1‑100 (MQ=255)                             cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGccc                                                                  <  2:353540/100‑1 (MQ=255)                                 tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCAttn                                                              <  2:1013748/100‑2 (MQ=255)                                   tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGaa                                                            >  2:2162641/1‑100 (MQ=255)                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                           <  2:70132/100‑1 (MQ=255)                                    cTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAAc                                                           <  2:1564263/100‑1 (MQ=255)                                      aaaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaa                                                         >  1:2190335/1‑100 (MQ=255)                                       aaaCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACaaa                                                        >  1:987096/1‑100 (MQ=255)                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                      <  1:704206/100‑1 (MQ=255)                                         aCAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATg                                                      <  1:2280377/100‑1 (MQ=255)                                          cAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTGCTGAGATCATACCCGCATTGATAGTTCGTTTACGCCCATTGAACAAATGc                                                     <  2:1312783/100‑1 (MQ=255)                                           aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCt                                                    <  1:131085/100‑1 (MQ=255)                                            aTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTa                                                   >  1:2201454/1‑100 (MQ=255)                                              aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                 <  2:1894835/100‑1 (MQ=255)                                              aCATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaa                                                 >  1:1701886/1‑100 (MQ=255)                                               cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                >  2:1252709/1‑100 (MQ=255)                                               cATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTaaaa                                                >  1:2083634/1‑100 (MQ=255)                                                   tACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTc                                            >  1:889609/1‑100 (MQ=255)                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                           <  2:276537/100‑1 (MQ=255)                                                    aCTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcg                                           <  2:1287817/100‑1 (MQ=255)                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAGTGGATAGTTCGTTTACGCCCATTGAACAAATGATAAAATTTcg                                           >  2:1951933/1‑99 (MQ=255)                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          <  2:262603/100‑1 (MQ=255)                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          <  2:889607/100‑1 (MQ=255)                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          <  2:1153808/100‑1 (MQ=255)                                                     cTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgc                                          >  2:1246780/1‑100 (MQ=255)                                                      tttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTcgcg                                         <  1:1012823/100‑1 (MQ=255)                                                        tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGcc                                       >  1:916387/1‑100 (MQ=255)                                                          tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                     <  2:916385/100‑1 (MQ=255)                                                          tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAg                                     <  2:1031338/100‑1 (MQ=255)                                                             gTCACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccg                                  >  2:24591/1‑100 (MQ=255)                                                               cACTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAgccgcc                                >  1:1568780/1‑100 (MQ=255)                                                                aCTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                               <  1:1964771/100‑1 (MQ=255)                                                                aCTGTCGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCa                               >  1:2276129/1‑100 (MQ=255)                                                                     cGTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAg                          >  1:834840/1‑100 (MQ=255)                                                                      gTTACTATATCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGa                         >  2:231271/1‑100 (MQ=255)                                                                           tataTCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                    <  1:1460723/100‑1 (MQ=255)                                                                           tataTCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                    >  2:101197/1‑100 (MQ=255)                                                                           tataTCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTc                    >  1:588284/1‑100 (MQ=255)                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                   <  2:2190325/100‑1 (MQ=255)                                                                            atatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                   <  2:1917774/100‑1 (MQ=255)                                                                             tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  1:732515/100‑1 (MQ=255)                                                                             tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  1:794038/100‑1 (MQ=255)                                                                             tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  1:24591/100‑1 (MQ=255)                                                                             tatCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                  <  2:834838/100‑1 (MQ=255)                                                                              atCGGCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                 <  1:19768/100‑1 (MQ=255)                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt               >  1:478814/1‑99 (MQ=255)                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              >  2:1722314/1‑100 (MQ=255)                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              >  1:631901/1‑100 (MQ=255)                                                                                 ggCTGAAATTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc              <  2:1774527/100‑1 (MQ=255)                                                                                      aaaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgag         <  1:2039528/100‑1 (MQ=255)                                                                                       aaTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGgaga        <  2:588285/100‑1 (MQ=255)                                                                                        aTTAATGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGAt       <  1:1252712/100‑1 (MQ=255)                                                                                           aaTGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCt    <  2:1560221/100‑1 (MQ=255)                                                                                             tGAGATCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCACCACGAAGATTTTCCTTATCAGGAGATCCTTc  >  2:1618040/1‑100 (MQ=255)                                                                                                 |                                                                                                TGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTC  >  NC_000913/2810663‑2810854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑