Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,888,225:1 +A 16.7% intergenic (+33/‑210) mnmE → / → tnaC tRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,888,2241.A16.7% 669.3 / 151.5 209intergenic (+32/‑211)mnmE/tnaCtRNA U34 5‑methylaminomethyl‑2‑thiouridine modification GTPase/tryptophanase leader peptide
Reads supporting (aligned to +/- strand):  ref base . (89/85);  new base A (17/18);  total (106/103)
Fisher's exact test for biased strand distribution p-value = 8.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.41e-02

Only 100 of 212 total aligned reads displayed.

CACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATT  >  NC_000913/3888126‑3888322
                                                                                                   |                                                                                                  
  ccGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATg                                                                                                 >  1:2023402/1‑100 (MQ=255)
   cGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                <  2:4184629/100‑1 (MQ=255)
   cGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGc                                                                                                <  2:1429299/100‑1 (MQ=255)
    gtggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCg                                                                                               <  1:2184977/98‑1 (MQ=255)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgc                                                                                             <  1:245090/100‑1 (MQ=255)
      ggAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgc                                                                                             <  1:2908661/100‑1 (MQ=38)
       gAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                            <  1:797957/100‑1 (MQ=38)
       gAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgct                                                                                            <  2:1250540/100‑1 (MQ=38)
        aaTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgctt                                                                                           <  2:3434700/100‑1 (MQ=38)
         aTTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttc                                                                                          <  1:2540359/100‑1 (MQ=255)
          tttACTTGAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                         <  1:1928508/100‑1 (MQ=255)
          tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                         <  2:801060/100‑1 (MQ=255)
          tttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcg                                                                                         <  1:3240287/100‑1 (MQ=255)
           ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgc                                                                                        <  2:2226102/100‑1 (MQ=255)
           ttACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcgt                                                                                        <  1:780023/100‑2 (MQ=37)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                     >  1:1552559/1‑100 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                     >  2:268572/1‑100 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGgcttcgcttc                                                                                     >  1:617094/1‑100 (MQ=255)
              cTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATCTGACAGTCAGAATGCGgcttcgtaag                                                                                     <  1:2807301/100‑5 (MQ=25)
                tCAGACGACCTGCTGGGGCGGATTTTGTCGAGCTGCTGTATTGGTAGGTAACTGCGCTTACGAAGCCGCATTGTGTCTGTTAGATCCGgcttcgc                                                                                        >  1:986155/1‑95 (MQ=255)
                tCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAt                                                                                   >  1:3277149/1‑100 (MQ=255)
                 cAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCAtt                                                                                  >  2:1080981/1‑100 (MQ=255)
                   gacgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTGCGAAGCCGCATCTGACAGTCAGAATGCGgcttcgtaagcgcgg                                                                                <  2:146558/100‑10 (MQ=18)
                     tctccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                              <  1:1660924/97‑1 (MQ=255)
                     cgacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTa                                                                              >  1:3697574/1‑100 (MQ=255)
                      gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  1:1825727/100‑1 (MQ=255)
                      gacCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  1:517770/100‑1 (MQ=255)
                      gacCTGCGCGTGCGGATTTTCTCGAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAc                                                                             <  1:4294487/100‑1 (MQ=255)
                       acCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                            <  1:1068817/100‑1 (MQ=255)
                        ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAcc                                                                            <  1:462786/99‑1 (MQ=255)
                        ccTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCa                                                                           >  2:3178121/1‑100 (MQ=255)
                         ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCAc                                                                          <  2:3134837/100‑1 (MQ=255)
                         ctgctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCAc                                                                          >  1:3993773/1‑100 (MQ=255)
                            ctgGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTcc                                                                       <  2:3975512/100‑1 (MQ=255)
                              ggggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAGCCGCGCTTACGAGGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                     <  2:3950472/100‑1 (MQ=255)
                              ggggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                     >  2:3892764/1‑100 (MQ=255)
                              ggggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTg                                                                     >  2:3279022/1‑100 (MQ=255)
                                ggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGtt                                                                   >  2:883747/1‑100 (MQ=255)
                                ggCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGtt                                                                   >  2:584333/1‑100 (MQ=255)
                                 gCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTa                                                                  >  1:293838/1‑100 (MQ=255)
                                  cGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAt                                                                 >  1:3378928/1‑100 (MQ=255)
                                   ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTTCTGTTAt                                                                 >  2:1515416/1‑99 (MQ=255)
                                   ggATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTAtt                                                                <  2:327391/100‑1 (MQ=255)
                                    gATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTc                                                               >  2:2708075/1‑100 (MQ=255)
                                     aTTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTcc                                                              <  2:2951757/100‑1 (MQ=255)
                                      ttttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCt                                                             <  2:3621657/100‑1 (MQ=255)
                                       tttCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTc                                                            <  1:2247898/100‑1 (MQ=255)
                                         gctcCAGCTGCTGTATTTGTATGTACTCTCGCGTCGGAAGCCGGATTCTGACTTTCAGATACCGCTTCGCTTCATTGTTACAACACCTGGTATTCCTCaa                                                          <  1:4388659/99‑1 (MQ=255)
                                           tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                        >  2:3812979/1‑100 (MQ=255)
                                           tcCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAcc                                                        >  1:2700208/1‑100 (MQ=255)
                                            ccAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATACGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAAccc                                                       >  1:586852/1‑100 (MQ=255)
                                               gCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                    >  2:2585815/1‑100 (MQ=255)
                                               gCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCttt                                                    >  1:2827081/1‑100 (MQ=255)
                                                cTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCtttt                                                   <  2:1799772/100‑1 (MQ=255)
                                                     gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                              >  1:1513206/1‑100 (MQ=255)
                                                     gTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCCTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                              >  1:953215/1‑100 (MQ=255)
                                                      tATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTaa                                              >  2:1384758/1‑99 (MQ=255)
                                                       aTTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAAc                                            <  2:1200521/100‑1 (MQ=255)
                                                        ttGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACa                                           >  1:4367487/1‑100 (MQ=255)
                                                          ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACAtt                                         >  1:121311/1‑100 (MQ=255)
                                                          ggTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGAAGCGGCTTCGCTTCATTGTTACCACTCCTATTAATCGTCAACCCTTTTTTTAAACAtt                                         >  1:1402572/1‑100 (MQ=255)
                                                            taagtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaa                                       <  1:2585605/100‑1 (MQ=255)
                                                              agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                     >  1:1427752/1‑100 (MQ=255)
                                                              agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                     >  2:4057053/1‑100 (MQ=255)
                                                              agtaaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTaaaa                                     <  1:4058048/100‑1 (MQ=255)
                                                                taaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAAtt                                   >  2:4350823/1‑100 (MQ=255)
                                                                 aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCTGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                  <  2:1142083/100‑1 (MQ=255)
                                                                 aaCCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTc                                  <  2:1642093/100‑1 (MQ=255)
                                                                  cttccgatctcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCt                                 <  1:1111218/90‑1 (MQ=25)
                                                                   ccGCGCTTACGAAGCCGTATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCtt                                <  1:3194463/100‑1 (MQ=255)
                                                                    cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTCAAATTCTTa                               >  1:4153342/1‑100 (MQ=255)
                                                                    cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTa                               >  1:1991595/1‑100 (MQ=255)
                                                                    cgcgCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTAAACATTAAAATTCTTAc                              <  2:4396615/100‑1 (MQ=255)
                                                                        cTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTa                           <  1:1801982/100‑1 (MQ=255)
                                                                         aagcGAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTATACATTATTATTTTTACg                             >  1:3752781/4‑97 (MQ=21)
                                                                          tACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAt                         <  1:3543952/100‑1 (MQ=255)
                                                                            cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                       >  2:2407898/1‑100 (MQ=255)
                                                                            cGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                       >  2:4128246/1‑100 (MQ=255)
                                                                             gAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                      >  2:908284/1‑100 (MQ=255)
                                                                             gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAAttt                       >  2:2019889/1‑99 (MQ=37)
                                                                             gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                      >  2:937407/1‑100 (MQ=38)
                                                                             gAAGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTta                      >  1:643789/1‑100 (MQ=38)
                                                                              aaGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtat                     >  1:819771/1‑100 (MQ=38)
                                                                               aGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtata                    >  2:3410766/1‑100 (MQ=38)
                                                                               aGCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTtata                    >  2:2787428/1‑100 (MQ=38)
                                                                                gCCGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATaa                   >  2:2392400/1‑100 (MQ=38)
                                                                                 ccGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAAt                  <  2:3002781/100‑1 (MQ=255)
                                                                                 ccGCATCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAAt                  >  2:4237334/1‑100 (MQ=38)
                                                                                  cGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATc                 >  2:218524/1‑100 (MQ=255)
                                                                                   gCATTCTGACTCTCAGATGTCTCTTCGCTTCATTGTTACCATTACTGTTATTCCTTAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAAt                  <  1:3519556/98‑1 (MQ=255)
                                                                                   cgcatCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATc                 >  1:2745626/5‑100 (MQ=38)
                                                                                       tCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTaa            <  2:1731906/100‑1 (MQ=255)
                                                                                       tCTGACAGTCAGAATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTa             >  2:1850659/1‑100 (MQ=255)
                                                                                             tGTCAGATGCGGCTTCGCTTCATTGTTCCCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg      <  1:2626548/100‑1 (MQ=255)
                                                                                             tGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg      <  2:3966688/100‑1 (MQ=255)
                                                                                             tGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAg      <  2:1672930/100‑1 (MQ=255)
                                                                                              gTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc     >  2:1103115/1‑100 (MQ=255)
                                                                                              gTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc     >  2:251680/1‑100 (MQ=255)
                                                                                              gTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc     >  2:3250698/1‑100 (MQ=255)
                                                                                              gTCAGATGCCGCTTCTCTTTATTGTTACCACTCCTGTTATTCCTCAACCCTTTATTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGc     <  1:1289722/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
CACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTAAGTAACCGCGCTTACGAAGCCGCATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTACCACTCCTGTTATTCCTCAACCCTTTTTTTAAACATTAAAATTCTTACGTAATTTATAATCTTTAAAAAAAGCATT  >  NC_000913/3888126‑3888322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: