Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,158:1 +A 17.3% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,1581.A17.3% 578.4 / 146.8 196intergenic (+23/+20)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base . (73/89);  new base A (14/20);  total (87/109)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.17e-01

Only 100 of 206 total aligned reads displayed.

TTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCA  >  NC_000913/120059‑120257
                                                                                                    |                                                                                                   
tttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTAc                                                                                                      <  2:1072613/100‑1 (MQ=255)
 ttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACg                                                                                                    <  2:1756389/100‑1 (MQ=255)
 ttCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACg                                                                                                    <  2:3774532/100‑1 (MQ=255)
  tCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGACTGCACTACTGACGCTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTAACCCTGTACgg                                                                                                   >  1:4245479/1‑100 (MQ=255)
   cAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACggg                                                                                                  <  2:3971802/100‑1 (MQ=255)
    aaTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGt                                                                                                 >  2:2848328/1‑100 (MQ=255)
     aTGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTg                                                                                                <  1:729520/100‑1 (MQ=255)
      tGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGa                                                                                               <  1:2130052/100‑1 (MQ=255)
       ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                              >  1:2761772/1‑100 (MQ=255)
       ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAg                                                                                              >  2:103839/1‑100 (MQ=255)
       ggCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAg                                                                                              <  2:1534278/100‑1 (MQ=255)
        gCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAgg                                                                                             <  1:138312/100‑1 (MQ=255)
         cGAAGAAATCCTCGTTCGTGGTCGGGGTGCTGATTGCACTACTGACGATTTACGGGGCGTTGGGGTCAAGATTATTGCCCTCACCCGTACAGGGTGAggg                                                                                            <  1:2638095/100‑1 (MQ=255)
         cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTTGTGTAAAGATTATTGCCCTCACCCAGTACGGGTGAggg                                                                                            <  1:2708905/100‑1 (MQ=255)
         cGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAggg                                                                                            <  2:1796924/100‑1 (MQ=255)
          gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  1:2451696/100‑1 (MQ=255)
          gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  2:3660599/100‑1 (MQ=255)
          gaagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  2:3583370/100‑1 (MQ=255)
          gaagaaAACCTCGTTCGTGGTCGTAGTGGTGATTGCACTACTGGCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCGTACAGGGTGAGGGc                                                                                           <  2:775415/100‑1 (MQ=255)
           aagaaAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCg                                                                                          <  2:2815417/100‑1 (MQ=255)
              aaaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                       >  1:2363370/1‑100 (MQ=255)
              aaaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTag                                                                                       <  2:197986/100‑1 (MQ=255)
               aaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTaga                                                                                      <  2:228650/100‑1 (MQ=255)
               aaaCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATCGCCCTCACCCGTACAGGGTGAGGGcaataa                                                                                      <  1:937173/100‑6 (MQ=37)
                 aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    <  2:167686/100‑1 (MQ=255)
                 aCCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    >  1:389127/1‑100 (MQ=255)
                  ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagagag                                                                                   >  2:831371/1‑100 (MQ=255)
                  ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATCATTGCCCTCACCCGTACAGGGTGAGGGcaataatct                                                                                   <  2:3111198/100‑9 (MQ=37)
                    tcgtttgtGGTCGTGGTGGTGATTGCTTTAGTGACGATTTACGGGGCGTGGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagaga                                                                                    <  1:1456883/97‑1 (MQ=255)
                    tcgttcgtGGTCGTGGTGGTGATTGGGCTCCTGACGATTTACGTGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTagag                                                                                     <  1:3571544/96‑1 (MQ=255)
                    tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAt                                                                                 <  2:3805443/100‑1 (MQ=255)
                    tcgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAt                                                                                 <  2:2885702/100‑1 (MQ=255)
                     cgttcgtGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGAtt                                                                                <  2:1577178/100‑1 (MQ=255)
                         cgtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                            <  2:1855016/100‑1 (MQ=255)
                          gtggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgc                                                                           >  1:650328/1‑100 (MQ=255)
                          ggggtagtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATg                                                                            <  1:2593696/97‑1 (MQ=255)
                           tggtcgtggtGGTGATTGCACTACTTACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcg                                                                          <  1:2069018/100‑1 (MQ=255)
                            ggtcgtggtGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATgcgc                                                                         <  2:2041808/100‑1 (MQ=255)
                                 tggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCttttt                                                                    >  2:1974014/1‑99 (MQ=255)
                                 tggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCCGTACGGGTGAGGGCGTAGAGAGACTAATGCGCTTTTa                                                                    >  1:695894/1‑100 (MQ=255)
                                  ggtggtgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTAc                                                                   >  1:2864845/1‑100 (MQ=255)
                                       tgATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGCGAGGGCGTAGAGAGATTAATGCCCTTTTGCGGCtt                                                              >  1:3453282/1‑100 (MQ=255)
                                         aTTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTg                                                            <  1:2191977/100‑1 (MQ=255)
                                          ttGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTgg                                                           <  2:1604005/100‑1 (MQ=255)
                                           tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                          <  2:1833678/100‑1 (MQ=255)
                                           tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGc                                                          >  1:1837684/1‑100 (MQ=255)
                                            gCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCACTCACCCTGTACGGGTGAGGGCGTAGATAGATTAATGCGCTTTTAAGGCTTTGGct                                                         >  1:2665031/1‑99 (MQ=255)
                                             cACTACTGACGATTTACGGGGCGTTGGTTTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCgg                                                        <  2:4142984/100‑1 (MQ=255)
                                               ctactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGtt                                                      <  2:3604567/100‑1 (MQ=255)
                                                 actGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGgttt                                                    >  1:539843/1‑100 (MQ=255)
                                                  ctGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtc                                                   <  2:3040842/100‑1 (MQ=255)
                                                   tGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtct                                                  >  2:1729138/1‑100 (MQ=255)
                                                    gACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTtctc                                                 >  2:3036456/1‑100 (MQ=255)
                                                     aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                <  2:637638/100‑1 (MQ=255)
                                                     aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                <  1:3199883/100‑1 (MQ=255)
                                                     aCGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTctct                                                <  2:4107268/100‑1 (MQ=255)
                                                        aTTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTa                                             <  1:285573/100‑1 (MQ=255)
                                                         tttACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGTCGGCTTTCCCTTGaa                                            >  1:1955392/1‑100 (MQ=255)
                                                           tACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAc                                          <  1:3587953/100‑1 (MQ=255)
                                                            aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                         >  2:1322055/1‑100 (MQ=255)
                                                            aCGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACa                                         <  1:3127186/100‑1 (MQ=255)
                                                               gggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGCTTAATGCGCTTTTACGGCTTTGGCGGGTTTGTCTTTAAACAGAt                                      >  1:3726572/1‑100 (MQ=255)
                                                                ggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATa                                     >  1:1064016/1‑100 (MQ=255)
                                                                ggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATa                                     <  2:1120949/100‑1 (MQ=255)
                                                                ggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATa                                     <  2:2761787/100‑1 (MQ=255)
                                                                ggCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCACTTTAAAAAGAtc                                     <  1:3265474/100‑2 (MQ=255)
                                                                  cGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGc                                   >  2:2102614/1‑100 (MQ=255)
                                                                   gTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGcc                                  <  2:3316475/100‑1 (MQ=255)
                                                                    ttGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGACGGCGTAGAGAGATTAATGCCCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                 <  1:2398880/100‑1 (MQ=255)
                                                                    ttGGTGTAAAGATTAGTGCCCTCACCCTGTACGGGTGAGGGCGTAGATAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCg                                 <  1:2517086/100‑1 (MQ=255)
                                                                       gtgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa                              >  2:4257020/1‑100 (MQ=255)
                                                                       gtgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATa                              <  2:194864/100‑1 (MQ=255)
                                                                        tgtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAc                             >  2:1157687/1‑100 (MQ=255)
                                                                         gtAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATAcc                            >  1:1205127/1‑100 (MQ=255)
                                                                           aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCt                           >  2:3137820/1‑99 (MQ=255)
                                                                           aaaGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTa                          <  1:2309482/100‑1 (MQ=255)
                                                                              gATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAaca                       >  2:2639699/1‑100 (MQ=255)
                                                                               ccgatctcccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAac                        <  1:3282151/91‑1 (MQ=255)
                                                                                  attGCCCTCACCCTGTACGGGTGAGGGCGTAGAGCGATTAATGCGCTTTTACGGCTTTGGCGGGTTTCTCTTTACACATATAGCCGATACCtca                         >  1:4299717/1‑92 (MQ=255)
                                                                                    cgCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGGTTTCTCTTTAAACCGATAGCCGATACTTAAAACGATc                  >  1:1341688/2‑99 (MQ=37)
                                                                                     gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa                 <  2:3726596/99‑1 (MQ=255)
                                                                                     gCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCa                 >  2:2563541/1‑99 (MQ=255)
                                                                                     gCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAGAGATAGCCGATACCTAACACGACCAg                >  1:1146781/1‑100 (MQ=255)
                                                                                     gCCCTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAg                >  2:4322065/1‑100 (MQ=255)
                                                                                      cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGCTACCTAACACGATCAGc               >  1:4067208/1‑100 (MQ=255)
                                                                                      cccTCACCCGTACAGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGc               >  2:474520/1‑100 (MQ=255)
                                                                                       ccTCACCCTGTGCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGATTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              >  2:2953398/1‑100 (MQ=255)
                                                                                       ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              >  1:808043/1‑100 (MQ=255)
                                                                                       ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              <  2:2345236/100‑1 (MQ=255)
                                                                                       ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              <  1:864772/100‑1 (MQ=255)
                                                                                       ccTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              <  2:1706101/100‑1 (MQ=255)
                                                                                         tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGcc              <  2:2203246/98‑1 (MQ=255)
                                                                                            cccTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATAcc         >  1:1397548/1‑100 (MQ=255)
                                                                                               tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg      <  2:1430324/100‑1 (MQ=255)
                                                                                               tGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCggg      <  2:2293631/100‑1 (MQ=255)
                                                                                                gTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGa     <  1:1097597/100‑1 (MQ=255)
                                                                                                 tACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGAt    >  2:4102527/1‑100 (MQ=255)
                                                                                                 tACGGGTGAGCGCGTAGAGAGATTAATGCGCTTTTCCGGGTTTCGCGGTTTTCTCTTTAAACAGATACCCGATACCTAACACGCTCAGCCATACCGGGAt    <  1:2996274/100‑1 (MQ=255)
                                                                                                  aCGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGAGAGCCGATACCTAACACGATCAGCCATACCGGGATc   <  1:3014188/100‑1 (MQ=255)
                                                                                                   cGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCa  <  2:3265521/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
TTTCAATGGCGAAGAAAACCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCA  >  NC_000913/120059‑120257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: