Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,499,365 A→T 16.9% Q143L (CAG→CTG)  rimL → ribosomal‑protein‑L7/L12‑serine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,499,3650AT16.9% 402.7 / 66.0 167Q143L (CAG→CTG) rimLribosomal‑protein‑L7/L12‑serine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (69/69);  new base T (18/10);  total (87/80)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 170 total aligned reads displayed.

TTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTAT  >  NC_000913/1499266‑1499464
                                                                                                   |                                                                                                   
 ttCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAg                                                                                                    >  2:2413415/1‑100 (MQ=255)
  tctcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCCAATGTCGGGTGGACAATCCGCAAAGCCACCAgg                                                                                                   >  1:3529333/1‑100 (MQ=255)
   ctcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGt                                                                                                  >  2:909553/1‑100 (MQ=255)
   ctcAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGt                                                                                                  >  2:208836/1‑100 (MQ=255)
        gcgcTGCAGGTATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCtt                                                                                             <  1:461760/100‑1 (MQ=255)
         cgcTGCAGGCATTGATTCATCATTACGCGCAGTCTGGCGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCttt                                                                                            <  1:1519418/100‑1 (MQ=255)
          gcTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTg                                                                                           >  1:1321796/1‑100 (MQ=255)
            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  2:4096174/100‑1 (MQ=255)
            tGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcg                                                                                         <  1:1837937/100‑1 (MQ=255)
             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGTACAATCCGCAAAGCGACCTGGTTGCTTTgcgg                                                                                        <  2:4174018/100‑2 (MQ=38)
             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcg                                                                                         <  1:3058953/99‑1 (MQ=39)
             gCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTgcgc                                                                                        <  2:3651704/100‑1 (MQ=255)
               aGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCGACCTGGTTGCTTTgcggat                                                                                      <  2:1870623/100‑4 (MQ=38)
                  cATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATgg                                                                                   >  2:2547433/1‑100 (MQ=255)
                   aTTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGt                                                                                  >  1:1783578/1‑100 (MQ=255)
                      gATACATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGTACAATCCGCAAAGGTGCCAGGTCGCTTTGCGCAATGGttt                                                                                <  1:359742/99‑1 (MQ=255)
                       aTTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                              >  1:1480324/1‑100 (MQ=255)
                       aTTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTa                                                                              <  1:4106615/100‑1 (MQ=255)
                        ttCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTAt                                                                             >  1:455740/1‑100 (MQ=255)
                          catcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATcc                                                                           <  1:3882764/100‑1 (MQ=255)
                           atcatTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCt                                                                          >  1:123961/1‑100 (MQ=255)
                               ttACGCCCAGTCTGGTGAACTTAGCCGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGaa                                                                      <  2:1082079/100‑1 (MQ=255)
                               ttACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGaa                                                                      <  1:1986249/100‑1 (MQ=255)
                                tACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCACATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAg                                                                     <  1:1064763/100‑1 (MQ=255)
                                 aCGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                    <  2:2244048/100‑1 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  2:1609392/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  1:2493055/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  2:1609588/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  2:1839189/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  2:1949676/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGt                                                                   >  1:1869697/1‑100 (MQ=255)
                                  cGCCCAGTCTGGTGAACTTAGACGCTTCGTCATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAgg                                                                    <  2:768705/99‑1 (MQ=255)
                                    cccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTGGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTGTATCCTTAAAGGtt                                                                  >  1:233968/1‑99 (MQ=255)
                                    cccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAGGGTTg                                                                 >  2:689633/1‑100 (MQ=255)
                                    cccAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTg                                                                 <  2:3140428/100‑1 (MQ=255)
                                       aGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCt                                                              <  2:1235332/100‑1 (MQ=255)
                                        gTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTg                                                             <  2:2119753/100‑1 (MQ=255)
                                         tCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTGGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGa                                                            <  1:2348793/100‑1 (MQ=255)
                                              tGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAg                                                       >  2:2815563/1‑100 (MQ=255)
                                               gAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAgg                                                      >  2:2024203/1‑100 (MQ=255)
                                               gAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAgg                                                      <  1:1136932/100‑1 (MQ=255)
                                                    tAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAg                                                 >  2:51479/1‑100 (MQ=255)
                                                        cGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTcc                                             >  2:4218234/1‑100 (MQ=255)
                                                           ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                          <  1:1922035/100‑1 (MQ=255)
                                                           ttCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGa                                          <  1:3543482/100‑1 (MQ=255)
                                                            tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                         <  2:3821503/100‑1 (MQ=255)
                                                            tCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGaa                                         <  2:2108709/100‑1 (MQ=255)
                                                               tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatga                                      >  1:4040137/1‑100 (MQ=255)
                                                               tGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatga                                      <  2:3842733/100‑1 (MQ=255)
                                                                gATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgat                                     <  1:1553476/100‑1 (MQ=255)
                                                                 aTCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                    <  2:2693401/100‑1 (MQ=255)
                                                                  tCAAATGTCGGGTGTACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAatgatg                                    <  1:3697731/99‑1 (MQ=255)
                                                                  tCAAATGTCGGGTGGACAATCCGCAAAGCAACCGGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGc                                   <  1:1690051/100‑1 (MQ=255)
                                                                  tCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGc                                   <  2:3697478/100‑1 (MQ=255)
                                                                   cAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGcc                                  <  2:455742/100‑1 (MQ=255)
                                                                    aggtGTCTGGTGGACAATCCGCAAAGCACCCAGGTCGCTGTGCTCTATGGTTTTCTCCGTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGcc                                  <  1:2921248/96‑1 (MQ=255)
                                                                     aaTGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTa                                >  1:172691/1‑100 (MQ=255)
                                                                       tGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatg                              <  2:1869703/100‑1 (MQ=255)
                                                                        gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                             <  2:3338216/100‑1 (MQ=255)
                                                                        gTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatga                             >  1:3767869/1‑100 (MQ=255)
                                                                           gtgTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatga                          <  1:519859/98‑1 (MQ=255)
                                                                           gggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatga                          <  2:285009/100‑1 (MQ=255)
                                                                           gggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatga                          >  2:1995415/1‑100 (MQ=255)
                                                                            ggTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgat                         >  2:1544130/1‑100 (MQ=255)
                                                                             gTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTatgatgatg                        <  2:3486594/100‑1 (MQ=255)
                                                                               ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                      <  2:2565779/100‑1 (MQ=255)
                                                                               ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                      >  2:3029050/1‑100 (MQ=255)
                                                                               ggACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCCGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGAtgtg                      >  2:2927427/1‑100 (MQ=255)
                                                                                 ccattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGaa                    >  1:2851631/7‑100 (MQ=38)
                                                                                  cattgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                   >  1:1162796/6‑100 (MQ=38)
                                                                                  cAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAAc                   >  2:652753/1‑100 (MQ=255)
                                                                                   attgcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATACTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACt                  >  1:2400107/5‑100 (MQ=38)
                                                                                    aTCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACtt                 >  1:1630370/1‑100 (MQ=255)
                                                                                     tgcGCAAAGCGACCTGGTTGCTTTGCGCCATGCTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTTCTGAATGATGCCTATGATGATGTGAACTta                >  1:1000593/3‑100 (MQ=37)
                                                                                      gcGCAAAGCGACCTGGTTGCTTTGCGCACTGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  1:719678/2‑100 (MQ=39)
                                                                                      gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  1:216108/2‑100 (MQ=39)
                                                                                      gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  1:111398/2‑100 (MQ=39)
                                                                                      gcGCAAAGCGACCTGGTTGCTTTGCGCAATGGATTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGCACTtat               >  2:1517933/2‑100 (MQ=38)
                                                                                      gcGCAAAGCGACCAGTTTGCTTTGCGCAATGGTACAATCCTTGAACGTTGCCTAAAACCGAACGAGATTCTGCATGATGCCAATAATGATCAGAACAta                >  1:1753651/2‑99 (MQ=255)
                                                                                      ccGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  1:2660625/1‑100 (MQ=255)
                                                                                      ccGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               <  2:1767668/100‑1 (MQ=255)
                                                                                      ccGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               <  2:4252759/100‑1 (MQ=255)
                                                                                       cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              >  1:1000978/1‑100 (MQ=255)
                                                                                       cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              >  2:3444713/1‑100 (MQ=255)
                                                                                       cGCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtat               >  2:2459295/1‑99 (MQ=255)
                                                                                       cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              <  1:555326/100‑1 (MQ=255)
                                                                                       cGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTtata              <  1:892285/100‑1 (MQ=255)
                                                                                        ncAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc             >  1:3141421/2‑100 (MQ=255)
                                                                                        gCAAAGCGACCTGGTTGCTTTGCGGAATGGTTTTATACTTGAAGGTTGCCTGAACGAGGCTGAGATCCTGAATGATGCTCATGATTATGTGAAGtt                 >  1:3027613/1‑96 (MQ=19)
                                                                                        gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc             >  2:1207623/1‑100 (MQ=255)
                                                                                        gCAAAGCGACCTGGTTGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAc             >  2:2695658/1‑100 (MQ=255)
                                                                                         cAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcg            <  2:2789712/100‑1 (MQ=255)
                                                                                             gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg        <  1:381362/100‑1 (MQ=255)
                                                                                             gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg        <  2:1605233/100‑1 (MQ=255)
                                                                                             gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg        >  1:155281/1‑100 (MQ=255)
                                                                                             gCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATAcgcgcg        >  1:2978696/1‑100 (MQ=255)
                                                                                               aaCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGta      >  1:428221/1‑100 (MQ=255)
                                                                                                aCCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtat     <  2:3061646/100‑1 (MQ=255)
                                                                                                  cAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtatta   >  2:695717/1‑100 (MQ=255)
                                                                                                   aGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGtattat  <  1:499634/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TTTCTCAGGCGCTGCAGGCATTGATTCATCATTACGCCCAGTCTGGTGAACTTAGACGCTTCGTGATCAAATGTCGGGTGGACAATCCGCAAAGCAACCAGGTCGCTTTGCGCAATGGTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTAT  >  NC_000913/1499266‑1499464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑