Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,674,955 A→T 17.2% intergenic (+8/+17) tqsA → / ← pntB pheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,674,9550AT17.2% 379.2 / 70.4 157intergenic (+8/+17)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (66/64);  new base T (15/12);  total (81/76)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 163 total aligned reads displayed.

TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGT  >  NC_000913/1674856‑1675054
                                                                                                   |                                                                                                   
tGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTca                                                                                                     <  1:877517/100‑1 (MQ=255)
  ccGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagc                                                                                                   >  2:3685547/1‑100 (MQ=255)
   cGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTcagca                                                                                                  <  2:3999640/100‑1 (MQ=255)
    gTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTctgctg                                                                                                 <  2:1587382/100‑3 (MQ=255)
            ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTc                                                                                         <  2:3888569/100‑1 (MQ=255)
            ttattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTc                                                                                         <  1:3590551/100‑1 (MQ=255)
             tattGTCAAAATTGCGCTTGAACAAACAGCGGGAGGCCAAAGCATCGCCGTTCTGTTAAGCGATCTAAATCAAGAGTGACGGCCTCAGCAGAGGCCGTc                                                                                         >  1:176844/1‑99 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  2:1270946/100‑2 (MQ=255)
              attGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAAAAAAGAGTGACGGCCTCTGCTGAGGCCGTCac                                                                                       <  1:4362045/100‑2 (MQ=255)
               ttGTGAAAATGGCGCTTGAACAAACAGCCGGAGCTCAACGCATCGCAGCTGTGTTAAGCGATCTCAATGAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                      <  1:364203/100‑3 (MQ=25)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                      <  1:1535681/100‑3 (MQ=255)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                      <  2:3958042/100‑3 (MQ=255)
               ttGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCact                                                                                      <  1:1535623/100‑3 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCTGCTGAGGCCGTCactc                                                                                     <  1:295799/100‑4 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                     <  1:637505/100‑1 (MQ=255)
                tGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTGCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                     <  1:2108472/100‑1 (MQ=255)
                 gTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAggg                                                                                     >  1:1035109/1‑99 (MQ=255)
                   cAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGATAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGCCAGGGTTa                                                                                  >  1:792738/1‑100 (MQ=255)
                      aaTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACag                                                                               <  1:4253169/100‑1 (MQ=255)
                       aTTGCGCTTGAACATACACCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATGTCAATGAAGAGTGACGGCCTCAGCAGAGGCCGTGAGGGTTACaga                                                                              <  1:3267306/100‑1 (MQ=255)
                       aTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAACGGGTGACGCCCTCAGCAGAGGCCGTCAGCGTTACa                                                                                >  1:1725898/1‑98 (MQ=255)
                       aTTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACaga                                                                              <  2:1823714/100‑1 (MQ=255)
                        ttGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACagag                                                                             <  1:4185609/100‑1 (MQ=255)
                         tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGc                                                                            <  2:497681/100‑1 (MQ=255)
                         tGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGc                                                                            <  1:300117/100‑1 (MQ=255)
                           cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                          <  2:1357415/100‑1 (MQ=255)
                           cgcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGAGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCtt                                                                          >  1:2438777/1‑100 (MQ=255)
                            gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                         >  2:740712/1‑100 (MQ=255)
                            gcTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCttt                                                                         <  2:1703696/100‑1 (MQ=255)
                             cTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTc                                                                        >  1:1414224/1‑100 (MQ=255)
                              ttGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCa                                                                       <  2:4115055/100‑1 (MQ=255)
                               tGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAg                                                                      <  1:137450/100‑1 (MQ=255)
                               tGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAg                                                                      <  1:135599/100‑1 (MQ=255)
                                 aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                    <  1:2280208/100‑1 (MQ=255)
                                 aacaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGa                                                                    >  2:3449554/1‑100 (MQ=255)
                                  acaaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAt                                                                   >  2:576927/1‑100 (MQ=255)
                                   caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                  >  1:2960379/1‑100 (MQ=255)
                                   caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                  >  2:2776591/1‑100 (MQ=255)
                                   caaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGAtt                                                                  <  2:80144/100‑1 (MQ=255)
                                    aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                 <  2:3237799/100‑1 (MQ=255)
                                    aaacaGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTg                                                                 <  1:4055631/100‑1 (MQ=255)
                                     aacaGCGGGAGGTGAAAGCATCGCCGTTCTGATAAGCGATCTAAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGc                                                                >  2:2975095/1‑100 (MQ=255)
                                         gCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTGAATAAAGAGTGCCGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATc                                                             <  1:1842554/99‑1 (MQ=255)
                                          cGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATGGCATCCa                                                           <  1:1854392/100‑1 (MQ=255)
                                            ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                         <  2:700221/100‑1 (MQ=255)
                                            ggAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACg                                                         >  2:1214432/1‑100 (MQ=255)
                                               ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTg                                                      <  1:377963/100‑1 (MQ=255)
                                               ggTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCAAGCTg                                                      >  1:2909256/1‑100 (MQ=255)
                                                 tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                    <  1:3512578/100‑1 (MQ=255)
                                                 tCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                    >  2:1043571/1‑100 (MQ=255)
                                                  cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGc                                                    <  2:386510/99‑1 (MQ=255)
                                                  cAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCt                                                   >  2:1134752/1‑100 (MQ=255)
                                                     aGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTg                                                >  1:1337374/1‑100 (MQ=255)
                                                      gCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                               >  2:2584437/1‑100 (MQ=255)
                                                      gCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                               >  2:600974/1‑100 (MQ=255)
                                                      acATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTgg                                               <  1:707184/99‑1 (MQ=255)
                                                       cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                              >  1:908481/1‑100 (MQ=255)
                                                       cATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGc                                              <  2:1953171/100‑1 (MQ=255)
                                                        aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                             <  2:2907020/100‑1 (MQ=255)
                                                        aTCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                             >  1:4366619/1‑100 (MQ=255)
                                                          caccGTTCTGTTACGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTc                                           >  2:2981705/3‑100 (MQ=255)
                                                           gcggTTCTGTTAAGCGATCTCAAGAGAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCg                                             <  1:1589733/94‑1 (MQ=255)
                                                           gCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCa                                          <  2:2283620/100‑1 (MQ=255)
                                                            ccGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTTGCTTTGGCGTCAc                                         <  2:2983243/100‑1 (MQ=255)
                                                               ttCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAAGATTGCATCCACGCTGGCTTTGGCGTCACCaa                                      <  1:2028565/100‑1 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   >  2:1667008/1‑100 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   <  1:3236342/100‑1 (MQ=255)
                                                                  tGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACa                                   >  1:2366504/1‑100 (MQ=255)
                                                                   gTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAg                                  >  2:4038988/1‑100 (MQ=255)
                                                                   gTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTGGGCGTCACCAAACAg                                  >  2:3104098/1‑100 (MQ=255)
                                                                     tAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCa                                >  2:2292134/1‑100 (MQ=255)
                                                                      aaGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAt                               >  1:741881/1‑100 (MQ=255)
                                                                       aGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtg                              <  2:1979566/100‑1 (MQ=255)
                                                                         cGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                            <  2:4097728/100‑1 (MQ=255)
                                                                          gttctcAATAAAGAGTGACGGCCTCAGCAGAGTCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCAtgtg                            <  2:1348595/97‑1 (MQ=255)
                                                                          gATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTgg                           >  2:930694/1‑100 (MQ=255)
                                                                           aTCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTggg                          <  2:3548078/100‑1 (MQ=255)
                                                                             ctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                        <  2:2205440/100‑1 (MQ=255)
                                                                             ctcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtg                        <  1:2292124/100‑1 (MQ=255)
                                                                              tcAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGgtgt                       >  1:1176690/1‑100 (MQ=255)
                                                                                aaTAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGttt                     >  2:3167513/1‑100 (MQ=255)
                                                                                 aTAAAGAGTGACGGCCTCAGCATAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTTGCTTTGGCGTCACCAAACAGCATGTGGGTGtttt                    <  2:4366685/100‑1 (MQ=255)
                                                                                  tAAAGATTGACGGCGTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGTATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTc                   <  1:1035369/100‑1 (MQ=255)
                                                                                   aaaGATTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                  <  2:2935587/100‑1 (MQ=255)
                                                                                    aaGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCt                 >  2:704142/1‑100 (MQ=255)
                                                                                     agagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                <  2:2438789/100‑1 (MQ=255)
                                                                                     agagTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                <  1:1178219/100‑1 (MQ=255)
                                                                                     agagTGACGGCCTCAGCAGAGGCCGTAAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCtt                >  2:2565283/1‑100 (MQ=255)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTcc                  >  2:4248258/4‑97 (MQ=39)
                                                                                      ccctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               >  1:2589842/4‑100 (MQ=39)
                                                                                       cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACNGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga              >  2:1222466/3‑100 (MQ=39)
                                                                                       cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga              >  2:2113381/3‑100 (MQ=255)
                                                                                       cctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTg               >  2:1741846/3‑99 (MQ=255)
                                                                                       agTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTga              <  2:2739643/100‑1 (MQ=255)
                                                                                        gTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTgaa             >  1:3738252/1‑100 (MQ=255)
                                                                                        ctGACGGCCTCTGCTGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCCCGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGCTTTc                   >  1:2599809/2‑94 (MQ=38)
                                                                                             ggCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACa        >  2:3058340/1‑100 (MQ=255)
                                                                                               ccTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGc      >  2:4315657/1‑100 (MQ=255)
                                                                                                cTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCg     <  2:780795/100‑1 (MQ=255)
                                                                                                 tCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCgg    <  1:882211/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TGCCGTTGACAATTATTGTCAAAATTGCGCTTGAACAAACAGCGGGAGGTCAAAGCATCGCCGTTCTGTTAAGCGATCTCAATAAAGAGTGACGGCCTCAGCAGAGGCCGTCAGGGTTACAGAGCTTTCAGGATTGCATCCACGCTGGCTTTGGCGTCACCAAACAGCATGTGGGTGTTTTCCTTGAAGAACAGCGGGT  >  NC_000913/1674856‑1675054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: