BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,756,529	A→T	100%	T278S (ACC→TCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,529	0	A	T	100.0%	394.8 / ‑6.7	118	T278S (ACC→TCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (46/70); total (47/71)

Only 100 of 118 total aligned reads displayed.

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTG  >  NC_000913/1756433‑1756624

gACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAAAAAAAAAAAAAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTccc                                                                                              <  1:4331776/100‑1 (MQ=255)

  ccTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAg                                                                                            >  2:4031010/1‑100 (MQ=255)

   cTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGa                                                                                           <  1:2284032/100‑1 (MQ=255)

    tGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATTTATACGTGCACGTAAAGTCGTTATCACTACGTCCCAGAt                                                                                          >  2:517102/1‑100 (MQ=255)

      ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGc                                                                                        <  2:1001810/100‑1 (MQ=255)

      ggTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGc                                                                                        <  2:3180905/100‑1 (MQ=255)

       gtgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCt                                                                                       <  1:3523795/100‑1 (MQ=255)

        tgtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTg                                                                                      <  1:2682738/100‑1 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                     <  1:129487/100‑1 (MQ=255)

         gtAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                     >  2:3730628/1‑100 (MQ=255)

         gatctaaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                     <  2:3488807/95‑1 (MQ=255)

          tAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGa                                                                                    <  1:1735261/100‑1 (MQ=255)

            gAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGAtt                                                                                  >  1:3513797/1‑100 (MQ=255)

              aaTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGagatc                                                                                >  1:3488789/1‑96 (MQ=255)

              aaTCCAGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAGTCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTgg                                                                                     <  1:3526326/95‑1 (MQ=255)

               aTCCCGGTAGAAGAATTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCa                                                                               <  2:268198/100‑1 (MQ=255)

               aTCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCa                                                                               <  2:1202010/100‑1 (MQ=255)

                 cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGTTCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATg                                                                             >  1:2339082/1‑100 (MQ=255)

                 cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGACAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATg                                                                             >  2:2564266/1‑100 (MQ=255)

                 cccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATg                                                                             <  1:3233580/100‑1 (MQ=255)

                  cccgTAGAAGAAGGTATATTCGCCTAGAAGATGATGATCGAAAAATGTAGCCGTGTACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCAt                                                                              <  1:229667/95‑1 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                            >  1:3174305/1‑100 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                            <  2:2246165/100‑1 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGa                                                                            <  2:286502/100‑1 (MQ=255)

                  ccGGTAGAAGAAGTTATCTTCGCCCAGAAAATAAAAATAAAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATg                                                                             <  1:2184499/99‑1 (MQ=255)

                   cGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAt                                                                           <  1:2564253/100‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          <  1:3879131/100‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          >  2:1430376/1‑100 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          <  1:3748001/100‑1 (MQ=255)

                    ggTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATc                                                                          <  2:3866614/100‑1 (MQ=255)

                    ggTAGAAGAAG‑TATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                         <  1:1751900/100‑1 (MQ=255)

                     gTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                         <  1:759497/100‑1 (MQ=255)

                      tAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCa                                                                         <  1:692862/99‑1 (MQ=255)

                      tAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCG‑TCCAGATGCTGGATTCCATGATCaaa                                                                       <  1:3265442/100‑1 (MQ=255)

                        gaagaaGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCaaaa                                                                      >  1:2241704/1‑100 (MQ=255)

                          agaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAc                                                                    >  2:1430870/1‑100 (MQ=255)

                           gaagTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAAcc                                                                   <  2:3975462/100‑1 (MQ=255)

                               ttATCTTCGCCCAGAAGATGATGATCTAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTTCGTCCCAGATGCTGTATTCCATGATCAAAAACCCa                                                                 <  1:1140532/98‑1 (MQ=255)

                               ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACg                                                               <  2:1454096/100‑1 (MQ=255)

                               ttATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACg                                                               <  2:1153963/100‑1 (MQ=255)

                                 aTCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGcc                                                             <  2:4377840/100‑1 (MQ=255)

                                  tCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGccc                                                            >  2:2707709/1‑100 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAACAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTAACAACTGCTGGATACCCTGATAAAAAACCTAccacc                                                            >  1:4335774/1‑94 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          >  2:2020301/1‑100 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          <  1:455394/100‑1 (MQ=255)

                                    ttCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGa                                                          >  1:4401557/1‑100 (MQ=255)

                                     tCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc                                                         <  1:571127/100‑1 (MQ=255)

                                      cGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                                                        <  2:1030724/100‑1 (MQ=255)

                                       gCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGAc                                                         <  1:3340899/98‑1 (MQ=255)

                                          cAGATGATGATGATCGAAAAATGTATCCGTGCAGGTAAATTCGTGATCACTGCGTCCCAGATGCTGGATTCCATGATTTAAAACCCACGCCCGACTcgcg                                                    <  1:3490360/100‑1 (MQ=255)

                                          cAGAAGATAATGAACGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgc                                                     <  1:2872838/99‑1 (MQ=255)

                                           agaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc                                                   <  1:1500714/100‑1 (MQ=255)

                                            gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                  >  2:247528/1‑100 (MQ=255)

                                            gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                  <  1:1963916/100‑1 (MQ=255)

                                            gaagaTGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCa                                                  >  2:250844/1‑100 (MQ=255)

                                                atgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAg                                              <  2:2950943/100‑1 (MQ=255)

                                                atgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAg                                              <  1:2633168/100‑1 (MQ=255)

                                                 tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                             >  1:439937/1‑100 (MQ=255)

                                                 tgatgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGc                                             >  2:1334388/1‑100 (MQ=255)

                                                   atgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCg                                           >  1:486579/1‑100 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          >  2:2332124/1‑100 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          >  1:2951253/1‑100 (MQ=255)

                                                    tgatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCgg                                          <  1:718499/100‑1 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         >  2:2552232/1‑100 (MQ=255)

                                                     gatCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGt                                         <  2:718957/100‑1 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATTACTGCGTCCCTGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                        <  1:2349975/100‑1 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                        <  2:1051028/100‑1 (MQ=255)

                                                      atCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTg                                        >  1:2016896/1‑100 (MQ=255)

                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCTTGATCACAAACCCACGCCATACTTGCGCAGAAGCCGGCGAc                                      >  1:3885325/1‑100 (MQ=255)

                                                        cGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGAc                                      <  1:824688/100‑1 (MQ=255)

                                                             aaTGTATCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGc                                 <  1:3212607/100‑1 (MQ=255)

                                                                  aTCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg                            >  2:1587625/1‑100 (MQ=255)

                                                                   tCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                           <  2:1307497/100‑1 (MQ=255)

                                                                   tCCGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                           >  1:4309471/1‑100 (MQ=255)

                                                                    ccGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                          >  1:4110416/1‑100 (MQ=255)

                                                                    ccGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGcc                          <  1:727398/100‑1 (MQ=255)

                                                                     cGTGCAGGTAACGTCGTTATCGCTGCGTCCGGGAGGCTAGATTCCATGGTCAAAGCCCCAGGCCGGACTCGCGCAGAAGCCGGTGACGGTGCAAACGcc                          <  1:3647312/99‑1 (MQ=255)

                                                                     cGTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                         <  2:1973695/100‑1 (MQ=255)

                                                                     cGTGCACGTAAAGCCGTTATCACTGCGTCCCAGATGCTGGATTCCATGAGCAACAGCCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCa                         <  1:572943/100‑1 (MQ=255)

                                                                     cGTGCACGCAAAGTCGTTATCACTGCGTCCCAGATGCTGGAAAAAAAAAAAAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                           <  1:669360/98‑1 (MQ=255)

                                                                      gTGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCAt                        <  2:2392034/100‑1 (MQ=255)

                                                                      gTGCACGTAAAGTCGTTATCACAGCGTCCCAAATGCTGGATTCCATGATCAAACACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGc                           <  1:3903853/97‑1 (MQ=255)

                                                                       tGCACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATc                       >  1:3797728/1‑100 (MQ=255)

                                                                         cACGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCt                     <  2:2259587/100‑1 (MQ=255)

                                                                          aCGTAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTc                    <  1:2161041/100‑1 (MQ=255)

                                                                             tAAAGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGAc                 <  2:161099/100‑1 (MQ=255)

                                                                               atatcattAGCACTGCGTCCCAGATGCTGGATGGCATAATCAAACACCCGCGCCCGACTCGCGAAGAAGCCGGTGACGTTGCAAACGCCATCCTc                    <  1:2766716/89‑1 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt              >  1:475069/1‑100 (MQ=255)

                                                                                aGTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGt              >  1:2114442/1‑100 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGTTGCCGTTGCAAACGCCATCCTCGACGGTa             >  2:2855703/1‑100 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa             <  2:2767303/100‑1 (MQ=255)

                                                                                 gTCGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTa             >  2:1167844/1‑100 (MQ=255)

                                                                                   cGTTATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGCCGTTGCAAACGCCATCCTCGACGGTACt           >  2:1460376/1‑100 (MQ=255)

                                                                                      tATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc        <  1:929763/100‑1 (MQ=255)

                                                                                      tATCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGAc        <  2:4160420/100‑1 (MQ=255)

                                                                                       aTCACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACg       <  1:1912022/100‑1 (MQ=255)

                                                                                         cACTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCa     >  2:3637877/1‑100 (MQ=255)

                                                                                          aCTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAg    >  1:4050115/1‑100 (MQ=255)

                                                                                          aCTGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGAAg    >  1:4191232/1‑100 (MQ=255)

                                                                                            tGCGTCCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTg  <  1:2332113/100‑1 (MQ=255)

GACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCATCCTCGACGGTACTGACGCAGTG  >  NC_000913/1756433‑1756624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`